4.5 Article

Cloning of lipoprotein lipase (LPL) and the effects of dietary lipid levels on LPL expression in GIFT tilapia (Oreochromis niloticus)

期刊

AQUACULTURE INTERNATIONAL
卷 21, 期 6, 页码 1219-1232

出版社

SPRINGER
DOI: 10.1007/s10499-013-9625-x

关键词

Lipoprotein lipase (LPL); Gene cloning and characterization; Hepatic LPL expression; Feed oil; Dietary lipid levels; GIFT (Oreochromis niloticus)

资金

  1. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fisher, Ministry of Agriculture, the People's Republic of China [BZ2007-06]
  2. Natural Science Foundation of the Jiangsu Higher Education Institutions of China [08KJD240003]
  3. Three Projects on aquaculture of Jiangsu Province [PJ2010-59]

向作者/读者索取更多资源

Genetically improved farmed tilapia (GIFT) (Oreochromis niloticus) is an important aquaculture species. Lipoprotein lipase (LPL) is considered as a key enzyme in lipid metabolism and deposition. The present study was conducted to investigate the nutritional regulation of LPL in GIFT. We cloned and characterized the LPL gene from GIFT. Finally, we determined the effects of dietary lipid levels and refeeding on hepatic LPL gene expression in GIFT. The LPL gene of GIFT (Oreochromis niloticus) (O.nLPL) was 2,298 bp in length and encoded 515 amino acids. Sequence analysis showed that O.nLPL shared 57.3-87.9 % identity with LPLs from other piscine species. To study LPL expression patterns, juveniles GIFT were fed diets containing 3.7, 7.7 or 16.6 % crude lipid for 90 days and the expression of hepatic O.nLPL was examined using real-time PCR. The abundance of hepatic LPL mRNA increased with increasing dietary lipid. The expression of O.nLPL mRNA in the 16.6 % dietary lipid group was significantly higher than that of the 3.7 % lipid group (P < 0.05). The expression of O.nLPL was increased in GIFT following a 48-h fast and decreased 12 h after refeeding. Hepatic LPL mRNA returned to fasting levels 48 h after refeeding. In summary, high dietary lipid induced expression of liver O.nLPL, and expression of liver LPL is regulated by fasting and refeeding.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据