期刊
AQUACULTURE
卷 322, 期 -, 页码 99-108出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquaculture.2011.09.026
关键词
Fish oil replacement; Alpha-linolenic acid (18:3n-3); Linoleic acid (18:2n-6); Desaturase; Elongase
资金
- Australian Research Council [DP1093570]
- Thailand Government
- Australian Research Council [DP1093570] Funding Source: Australian Research Council
This study aimed to gain a better understanding of the metabolic fate of dietary fatty acids in rainbow trout, with a specific focus on the effect of varying total C-18 PUFA level. Fish were fed a control fish oil based diet or one of five experimental fish oil deprived diets formulated with a constant 1/1 ratio of 18:3n-3/18:2n-6 and varying total C-18 PUFA levels for a period of 7 weeks. The transcriptional changes of the Delta-6 desaturase and elongase enzymes in direct comparison to in vivo fatty acid bioconversion, estimated using the whole-body fatty acid balance method, were analysed. The main findings were that i) the efficiency of Delta-6 desaturase was negatively affected by C-18 PUFA availability, but the total apparent in vivo enzyme activity was directly proportional to C-18 PUFA substrate availability; ii) Delta-6 desaturase had a greater affinity towards n-3PUFA than n-6PUFA; iii) excessive C-18 PUFA substrate availability could limit the availability of Delta-6 desaturase to act on C-24 fatty acid; iv) the elimination of dietary n-3LC-PUFA (enzyme products) up-regulated the transcription rate of Delta-6 desaturase; but v) the total apparent in vivo enzyme activity was directly and positively affected by substrate availability, and not product presence/absence nor the extent of the enzyme transcription rate. (C) 2011 Elsevier B.V. All rights reserved.
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