期刊
AQUACULTURE
卷 319, 期 1-2, 页码 188-193出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2011.06.018
关键词
Aerobic denitrification; Bacillus coagulans; Nitrite; Nitrite nitrogen degradation rate; Aquaculture
资金
- Special Fund for Agro-scientific Research in the Public Interest [nyhyzx07-04]
- Earmarked Fund for China Agriculture Research System [NYCYTX-49-17]
In the present experiment, eighteen aerobic denitrifying Bacillus strains were isolated by BTB plate technique. The Bacillus sp. strain YX-6 was selected for the aerobic denitrification studies because it is highly effective in removing nitrite compared with other strains of Bacillus sp. The results indicated that the strain YX-6 could degrade the nitrite nitrogen (nitrite-N) from 10 mg/L to zero in 14 h and the nitrite-N degradation rate was approximately to 100% at the DO concentration of 5.2-5.8 mg/L Furthermore, the aerobic denitrification of the strain YX-6 was significantly higher than those of the positive controls under different pH, temperature, nitrite and salinity concentrations (p < 0.05). The amplification of nitrite reductase confirmed that the strain YX-6 contained the nirS gene. According to the morphological observation and 16SrRNA gene analysis, the strain YX-6 was identified as Bacillus coagulans. (C) 2011 Published by Elsevier B.V.
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