4.7 Article

Laser- and UV-assisted modification of polystyrene surfaces for control of protein adsorption and cell adhesion

期刊

APPLIED SURFACE SCIENCE
卷 255, 期 10, 页码 5453-5457

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.apsusc.2008.08.053

关键词

Laser; Ablation; Modification; Cell adhesion; Wetting; Polystyrene

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  1. NANOMIKRO

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An appropriate choice of laser and process parameters enables new approaches for the fabrication of polymeric lab-on-chip devices with integrated functionalities. We will present our current research results in laser-assisted modi. cation of polystyrene ( PS) with respect to the fabrication of polymer devices for cell culture applications. For this purpose laser micro-patterning of PS and subsequent surface functionalization was investigated as function of laser and process parameters. A high power ArF-excimer laser radiation source with a pulse length of 19 ns as well as a high repetition ArF-excimer laser source with a pulse length of 5 ns were used in order to study the influence of laser pulse length on laser-induced surface oxidation. The change in surface chemistry was characterized by X-ray photoelectron spectroscopy and contact angle measurements. The difference between laser-assisted modi. cation versus UV-lamp assisted modi. cation was investigated. A photolytic activation of specific areas of the polymer surface and subsequent oxidization in oxygen or ambient air leads to a chemically modified polymer surface bearing carboxylic acid groups well-suited for controlled competitive protein adsorption or protein immobilization. Finally, distinct areas for cell growth and adhesion are obtained. (C) 2008 Elsevier B. V. All rights reserved.

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