Nanostructured bioglass thin films synthesized by pulsed laser deposition: CSLM, FTIR investigations and in vitro biotests

We report the synthesis by pulsed laser deposition of thin structures of two bioactive glasses belonging to the SiO2-Na2O-K2O-CaO-MgO-P2O5 system, on medical grade Ti substrates. We evaluated their biocompatibility after immersion in simulated body fluids and by performing cells adhesion tests. The Films were characterized by confocal scanning laser microscopy and Fourier transform infrared spectrometry, before and after 30 and 46 days immersion in fluids. Our studies demonstrated that deposited coatings were degraded in simulated fluids. A new apatite layer was synthesized by ions changing with the fluid during the decomposition of bioglasses. We investigated after immersion in fluids cells adhesion and the cytoskelet organization of synthesized structures, by fluorescence microscopy. A good adhesion to bioglass coatings was evidenced. (C) 2008 Elsevier B.V. All rights reserved.