期刊
APPLIED SOIL ECOLOGY
卷 63, 期 -, 页码 105-111出版社
ELSEVIER
DOI: 10.1016/j.apsoil.2012.09.010
关键词
Rhizobia; Bacterial biomass; Growth inhibition; Polycyclic aromatic hydrocarbons; PAH degradation
类别
资金
- National Council of Science and Technology (CONACYT, Mexico) through the SEP-CONACYT grant [79456]
- CONACyT
Polycyclic aromatic hydrocarbons (PAH) are ubiquitous pollutants that are toxic and recalcitrant to degradation by bacteria. This research evaluated the toxicity of different concentrations [10, 20, 40, 60, 80 and 100 mu g mL(-1)] of phenanthrene (PHE) or benzo[a]pyrene (BaP) on the growth of Rhizobium tropici CIAT899 under in vitro conditions as well as the potential degradation of PHE and BaP by this bacterium. At 24 h, a 40% decrease in Rhizobium growth was observed when exposed to 40 mu g mL(-1) of either PHE or BaP. Furthermore, bacterial growth was completely inhibited by PHE or BaP applied in 80 and 100 mu g mL(-1). After 96 h, the growth of R. tropici at 40 mu g PHE mL(-1) or 60 mu g BaP mL(-1) was similar to those treatments without PAH. To evaluate R. tropici degrading capabilities, supernatants of cultures with 40 mu g PHE mL(-1) or 60 mu g BaP mL(-1) were analyzed by gas chromatography coupled to mass spectrophotometer (GC-MS). R. tropici was able to degrade either PHE or BaP diminishing its concentration in 20% and 25% during the first 24 h, degradation obtained at 120 h was 50% and 45% for PHE or BaP, respectively. This research shows for the first time that R. tropici CIAT 899 grows in liquid culture medium contaminated with PAH, and moreover is able to growth and to degrade either PHE or BaP. (c) 2012 Elsevier B.V. All rights reserved.
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