Article
Biochemistry & Molecular Biology
Ksenia A. Zaripova, Ekaterina P. Kalashnikova, Svetlana P. Belova, Tatiana Y. Kostrominova, Boris S. Shenkman, Tatiana L. Nemirovskaya
Summary: The findings suggest that PANX1 ATP-permeable channels play a crucial role in regulating muscle atrophy processes by modulating the expression of E3 ligases, as well as protein translation and elongation processes during unloading.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Yejin Ahn, Kyungae Jo, Yeok Boo Chang, Hyung Joo Suh
Summary: This study found that yeast hydrolysate can promote muscle cell differentiation and inhibit muscle atrophy. In cell and mouse models, yeast hydrolysate increased the length and area of muscle fibers and significantly increased the expression of muscle differentiation-related genes. Furthermore, yeast hydrolysate also reduced the expression of muscle atrophy-related proteins and improved grip strength and lean mass.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Medicine, Research & Experimental
Hong Wang, Haizhou Wang, Xueyan Li, Wen Xu
Summary: This study reveals a novel molecular mechanism underlying MuRF-1-mediated internal laryngeal muscle denervated atrophy, in which MuRF-1 promotes the disequilibrium of the G/F-actin ratio by regulating G-actin ubiquitination.
Article
Biochemistry & Molecular Biology
Kristina Sharlo, Irina Lvova, Sergey Tyganov, Ksenia Zaripova, Svetlana Belova, Tatiana Kostrominova, Boris Shenkman, Tatiana Nemirovskaya
Summary: Skeletal muscle abnormalities and atrophy during unloading can be attributed to excess calcium accumulation, which may be caused by the inhibition of SERCA activity. Treating with SERCA activator CDN1163 can reduce calcium level, thus preventing negative consequences. In this study, unloading for 7 days led to increased fatigue, decreased mitochondrial markers and slow-type myosin, and disrupted calcium homeostasis in Soleus muscle. These changes were prevented by SERCA activation but had no effect on muscle atrophy.
Article
Plant Sciences
Takahito Takase, Satoshi Ikeuchi, Takashi Inoue, Rie Mukai
Summary: The study found that lemon peel extract containing eriocitrin can suppress muscle atrophy and reduce the expression of ubiquitin ligase genes through its antioxidative effect, preventing disuse muscle atrophy caused by oxidative stress.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Food Science & Technology
Young-Sool Hah, Won Keong Lee, Sangyeob Lee, Jin-Hee Seo, Eun Ji Kim, Yeong-in Choe, Sang Gon Kim, Jun-Il Yoo
Summary: Sarcopenia, the loss of muscular mass and strength with aging, leads to functional impairment, increased risk of falls and fractures, and loss of independence. Coumestrol has been found to inhibit skeletal muscle loss, but its protective mechanism is poorly understood. This study investigated the protective effect of coumestrol against dexamethasone-induced muscle atrophy using in vitro and in vivo analyses. The results suggest that coumestrol may attenuate dexamethasone-induced muscle atrophy by blocking the ubiquitin-proteasome pathway through suppressing AMPK-FoxO1/3 signaling. Therefore, coumestrol could be a potential treatment for aging sarcopenia.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Nutrition & Dietetics
Young-Sool Hah, Won Keong Lee, Sangyeob Lee, Eun Ji Kim, Jung Hyeon Lee, Seung-Jun Lee, Yeong Ho Ji, Sang Gon Kim, Hyeong-Hwan Lee, Seo Yeon Hong, Jun-Il Yoo
Summary: Sarcopenia is the decline in muscle mass and strength with age, resulting in impaired daily functions and increased risk of falls and fractures. β-sitosterol has shown potential to prevent muscle loss and has a protective effect on muscle mass in mice and cell models.
Article
Cell Biology
Lili Feng, Bowen Li, Yue Xi, Mengxin Cai, Zhenjun Tian
Summary: This study found that aerobic exercise and resistance exercise effectively alleviated skeletal muscle atrophy in MI mice by regulating the IGF-1 pathway. Exercise promoted myogenesis, protein synthesis, reduced protein degradation, and cell apoptosis.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Johan Lindqvist, Justin Kolb, Josine de Winter, Paola Tonino, Zaynab Hourani, Siegfried Labeit, Coen Ottenheijm, Henk Granzier
Summary: Nemaline myopathy is a disease characterized by skeletal muscle weakness and atrophy. This study found that levels of the protein MuRF1 are significantly increased in patients with nebulin-based Nemaline myopathy and mouse models of the disease. Knocking out the MuRF1 gene in mouse models led to increased muscle size but did not improve muscle function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Svetlana P. Belova, Ekaterina P. Kalashnikova, Sergey A. Tyganov, Tatiana Y. Kostrominova, Boris S. Shenkman, Tatiana L. Nemirovskaya
Summary: This study evaluated the effects of maintaining muscle tone during unloading through tetanus toxin (TeNT) treatment on the regulation of atrophic processes. The results showed that TeNT treatment can reduce the decline in muscle strength and affect the expression of MyHC Iβ. These effects are mediated by signaling pathways regulating protein synthesis and degradation.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
David P. McBey, Michelle Dotzert, C. W. J. Melling
Summary: This study investigated glycogen and fat content in skeletal muscle fibres of diabetic rats following exercise training. The results indicate that insulin treatment and exercise training have significant effects on muscle fibre types and lipid content.
LIPIDS IN HEALTH AND DISEASE
(2021)
Article
Endocrinology & Metabolism
Bruno Vecchiatto, Rafael C. da Silva, Talita S. Higa, Cynthia R. Muller, Anna Laura Americo, Vanessa C. Fortunato-Lima, Marilia M. Ferreira, Luiz Felipe Martucci, Miriam H. Fonseca-Alaniz, Fabiana S. Evangelista
Summary: Aerobic physical training can improve obesity-linked insulin resistance by modifying skeletal muscle phenotype independently of changes in the muscle ACE2/Ang 1-7/Mas axis.
DIABETOLOGY & METABOLIC SYNDROME
(2021)
Article
Geriatrics & Gerontology
Melissa L. Borg, Julie Massart, Thais De Castro Barbosa, Adria Archilla-Ortega, Jonathon A. B. Smith, Johanna T. Lanner, Jorge Alsina-Fernandez, Benjamin Yaden, Alexander E. Culver, Hakan K. R. Karlsson, Joseph T. Brozinick, Juleen R. Zierath
Summary: The study showed that modified UCN2 peptides were effective in combating skeletal muscle wasting associated with obesity and immobilization. The treatment increased muscle weight, regulated protein synthesis and degradation pathways, enhanced muscle strength, and altered the expression of relevant genes.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2021)
Article
Immunology
Vitor R. Munoz, Rafael C. Gaspar, Matheus B. Severino, Ana P. A. Macedo, Fernando M. Simabuco, Eduardo R. Ropelle, Dennys E. Cintra, Adelino S. R. da Silva, Young-Bum Kim, Jose Rodrigo Pauli
Summary: Physical exercise can restore the levels of RhoA and ROCK2 proteins in the skeletal muscle of obese animals, increase phosphorylation of IRS1 and Akt proteins, and decrease levels of inhibitory regulators such as PTEN and PTP-1B. These findings suggest that exercise-induced upregulation of the RhoA-ROCK2 signaling pathway in skeletal muscle is associated with improved systemic insulin sensitivity in obese mice.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jongsoo Mok, Tae Sub Park, Sunhong Kim, Daehoon Kim, Cheol Soo Choi, Joonghoon Park
Summary: The study found that Prokr1 plays an important role in skeletal muscle, improving insulin sensitivity and being a potential therapeutic target for ameliorating insulin resistance in skeletal muscle.
Article
Physiology
Marcus Moberg, William Apro, Oscar Horwath, Gerrit van Hall, Sarah Joan Blackwood, Abram Katz
Summary: Acute severe hypoxia inhibits signaling pathways involved in muscle growth regulation, including mTORC1 and JNK signaling. These effects do not appear to be mediated by changes in the degree of metabolic stress in the muscle.
Article
Endocrinology & Metabolism
Sarah J. Blackwood, Oscar Horwath, Marcus Moberg, Marjan Ponten, William Apro, Maria M. Ekblom, Filip J. Larsen, Abram Katz
Summary: Muscle fiber composition differences are associated with alterations in peripheral insulin action and secretion, which can be used to predict insulin resistance and glucose-stimulated insulin secretion.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Endocrinology & Metabolism
Oscar Horwath, Marcus Moberg, Angelica Linden Hirschberg, Bjorn Ekblom, William Apro
Summary: Moderate-dose testosterone administration has no effect on molecular regulators of muscle protein turnover and mitochondrial remodeling in young women.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Neurosciences
Oscar Horwath, Sebastian Edman, Alva Andersson, Filip J. Larsen, William Apro
Summary: Fibre type-specific analyses are important for understanding muscle physiology, but are difficult and time-consuming. A rapid and valid method called THRIFTY was developed for high-throughput fibre typing. THRIFTY showed excellent agreement with a previously validated protocol and produced pure fibre pools. It was also significantly faster than the traditional dot blot method. THRIFTY is a novel and versatile method for rapid fibre typing, which can facilitate extensive studies on skeletal muscle physiology.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Cell Biology
Fabian Nordstrom, Rasmus Liegnell, William Apro, Sarah J. Blackwood, Abram Katz, Marcus Moberg
Summary: GPR81 is a protein receptor that binds to L-lactate and inhibits cAMP-PKA-CREB signaling pathway. This study investigates the expression of GPR81 and related signaling in human skeletal muscle under conditions of elevated lactate levels.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Physiology
Mikael Flockhart, Dominik Tischer, Lina C. Nilsson, Sarah J. Blackwood, Bjoern Ekblom, Abram Katz, William Apro, Filip J. Larsen
Summary: The study aimed to investigate the effects of different types of exercise on glucose tolerance, assess the potential differences between two exercise paradigms in relation to changes in mitochondrial function, and determine if endurance athletes differ from nonendurance-trained controls in their metabolic responses. The results showed that prolonged exercise reduces glucose tolerance and increases insulin resistance in endurance athletes the following day. These findings are associated with an increased lipid load, high lipid oxidation capacity, and increased fat oxidation.
Article
Endocrinology & Metabolism
Sarah J. Blackwood, Oscar Horwath, Marcus Moberg, Marjan Ponten, William Apro, Maria M. Ekblom, Filip J. Larsen, Abram Katz
Summary: This study examined the effect of muscle fiber composition on metabolic responses to a 3-day fast. The results showed that the group with a higher proportion of type I muscle fibers had a greater loss of insulin sensitivity and a higher capacity to oxidize lipids after starvation.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Oscar Horwath, Fabian Nordstrom, Ferdinand von Walden, William Apro, Marcus Moberg
Summary: Cumulative evidence supports the hypothesis that hypoxia acts as a regulator of muscle mass. This study examined the effect of hypoxia on signaling pathways and gene expression in human skeletal muscle after resistance exercise. The results showed that hypoxia attenuated ribosome signaling but did not affect satellite cell pool expansion and myogenic gene expression.
Article
Physiology
Mats Lilja, Marcus Moberg, William Apro, Luis Manuel Martinez-Aranda, Hakan Rundqvist, Billy Langlet, Thomas Gustafsson, Tommy R. Lundberg
Summary: We investigated the effects of ibuprofen and low-dose aspirin on muscle hypertrophy in young adults undergoing resistance training. The study found that there were no significant differences in molecular markers, muscle fiber size, ribosome biogenesis, satellite cell content, myonuclear accretion, and angiogenesis between the two groups. However, the mRNA levels of atrogin-1 and MuRF-1 were more downregulated in the low-dose aspirin group after acute exercise. These findings suggest that the previously reported deleterious effects of high doses of ibuprofen on muscle hypertrophy may not be explained by these established hypertrophy regulators.
JOURNAL OF APPLIED PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
M. Flockhart, L. C. Nilsson, E. N. Tillqvist, F. Vinge, F. Millbert, J. Lannerstrom, P. H. Nilsson, D. Samyn, W. Apro, M. L. Sundqvist, F. J. Larsen
Summary: Oxidative stress plays a vital role in the adaptive responses to physical training. Glucosinolates from cruciferous vegetables can be converted into antioxidative compounds that activate crucial antioxidant defence pathways and improve mitochondrial function and protein integrity. In this study, combining glucosinolate-rich broccoli sprouts with exercise significantly reduced protein oxidation levels, improved physical performance, and mitigated nocturnal hypoglycaemia.