期刊
APPLIED PHYSICS LETTERS
卷 99, 期 16, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.3651291
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资金
- Research Fellowships for Young Scientists
- Japan Society for the Promotion of Science [20310075]
- Grants-in-Aid for Scientific Research [20310075, 10J00294, 22120010, 20310069] Funding Source: KAKEN
In-situ guidance of neuronal processes (neurites) is demonstrated by applying wet femtosecond-laser processing to an organosilane self-assembled monolayer (SAM) template. By scanning focused laser beam between cell adhesion sites, on which primary neurons adhered and extended their neurites, we succeeded in guiding the neurites along the laser-scanning line. This guidance was accomplished by multiphoton laser ablation of cytophobic SAM layer and subsequent adsorption of cell adhesion molecule, laminin, onto the ablated region. This technique allows us to arbitrarily design neuronal networks in vitro. (C) 2011 American Institute of Physics. [doi:10.1063/1.3651291]
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