期刊
APPLIED PHYSICS B-LASERS AND OPTICS
卷 107, 期 2, 页码 375-378出版社
SPRINGER
DOI: 10.1007/s00340-012-4938-4
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资金
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao Arthur Bernardes-Universidade Federal de Vicosa (FUNARBE-UFV)
- Nucleo de Excelencia em Fisica Biologica-Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (PRONEX-FACEPE)
We present a simple method to calibrate optical tweezers by using only videomicroscopy to measure the position autocorrelation function of the trapped bead in the potential well of the tweezers. To accomplish this task, we use a high-speed camera with similar to 500 fps (frames per second), which provides a precise measurement of the relaxation time of the bead Brownian fluctuations. We also study the variation of the trap stiffness as a function of some parameters of interest such as the laser power and the distance from the bead center to the microscope coverslip, showing that the presented method returns precise results.
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