Article
Optics
Leonardo Andre Ambrosio, Jiajie Wang, Gerard Gouesbet
Summary: Based on the adjoint boundary value problem proposed by Zulehner and Rohatschek [1] decades ago, analytic and closed-form expressions for the photophoretic forces exerted by arbitrary-shaped beams on homogeneous and low-loss spherical particles are derived in both the free molecular and slip flow regimes. The asymmetry vector for arbitrary refractive index particles is explicitly calculated by expanding the internal electromagnetic fields using the generalized Lorenz-Mie theory (GLMT). The proposed approach is the first systematic attempt to incorporate GLMT into the field of photophoresis and may be extended to spheroids and find important applications in optical trapping and manipulation of microparticles, geoengineering, particle levitation, optical trap displays, and other areas.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2022)
Review
Optics
Luiz Felipe Votto, Abdelghani Chafiq, Gerard Gouesbet, Leonardo Andre Ambrosio, Abdelmajid Belafhal
Summary: The Ince-Gaussian beams are included in the framework of the generalized Lorenz-Mie theory through their expansions in terms of Laguerre-Gaussian beams. The beam shape coefficients are derived based on the expressions of Laguerre-Gaussian beam shape coefficients. This allows for a more straightforward computation of the coefficients and an analysis of the implications of the finite series method in the context of the GLMT framework.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2023)
Article
Optics
Leonardo A. Ambrosio, Jhonas O. de Sarro, Gerard Gouesbet
Summary: This study derives a polychromatic version of the generalized Lorenz-Mie theory stricto sensu (GLMT) by expanding arbitrary time-dependent fields into partial waves using Bromwich scalar potentials. The new formalism introduces field shape spectra (FSSs) which are intrinsically frequency-dependent, modifying and redefining the physical quantities expressed in the monochromatic GLMT.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2024)
Article
Optics
Luiz Felipe Machado Votto, Gerard Gouesbet, Leonardo Andre Ambrosio
Summary: Due to recent developments in generalized Lorenz-Mie theories (GLMTs), there is a renewed interest in the finite series (FS) method. However, the lack of flexibility in its earlier statements has led to its neglect since the 1990s. By dissecting the later works and exploring possibilities for generalization, simplification, and organization, a more accessible formulation of the FS method has been proposed.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2023)
Article
Optics
Leonardo A. Ambrosio, Gerard Gouesbet
Summary: The relationship between the Rayleigh limit of the generalized Lorenz-Mie theory (GLMT) and the dipole theory of forces is investigated, revealing extreme accuracy between the two approaches up to 3000 decimal places, reinforcing the conjecture that the Rayleigh limit of the GLMT might exactly identify with the traditional dipole theory of forces. However, mathematically uncovering such identification remains to be done.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2021)
Article
Optics
Gerard Gouesbet
Summary: The study examines the optical forces exerted by off-axis Bessel beams on Rayleigh particles, focusing on the existence of axicon forces in addition to the classical scattering and gradient forces. Results obtained in the more restricted case of on-axis beams confirm previous findings and help clarify their meaning, while also addressing the distinction between dark and non-dark beams. The question of whether the generalized Lorenz-Mie theory for Rayleigh particle aligns with the usual dipole theory is also raised.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2021)
Review
Optics
Leonardo Andre Ambrosio
Summary: This paper presents an analytical approach to incorporate zero-order continuous frozen waves (FWs), a specific class of non-diffracting beams, into the framework of the generalized Lorenz-Mie theory (GLMT). The approach resolves two main drawbacks observed in previous work, which are related to the computation of beam shape coefficients. Examples and field reconstructions are provided for FWs with different polarizations. The approach is significant for using continuous FWs as alternative laser beams and accurately calculating their optical properties in the field of light scattering. (c) 2022 Elsevier Ltd. All rights reserved.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2023)
Article
Optics
Luiz Felipe Votto, Leonardo Ambrosio, Gerard Gouesbet, Jiajie Wang
Summary: This paper presents a thorough study of the mathematical and computational nuances of the Finite Series (FS) method for Lens-Focused Laguerre-Gaussian beams, comparing it with other known methods and concluding that a properly implemented FS algorithm is generally the most preferable option.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2021)
Article
Optics
Leonardo A. Ambrosio, Gerard Gouesbet
Summary: This study establishes a connection between the Rayleigh limit of the generalized Lorenz-Mie theory and the transverse forces in the Rayleigh limit of the dipole theory, revealing the origin of spin-curl forces in terms of couplings between beam shape coefficients of distinct order and their relationship with what has recently been called nonstandard scattering forces.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2021)
Article
Optics
Leonardo A. Ambrosio, Vinicius S. de Angelis, Gerard Gouesbet
Summary: This study complements previous work on optical forces and force categorizations on dipole particles with electric and magnetic properties in the generalized Lorenz-Mie theory. It establishes a rigorous connection between the dipole limit of the GLMT and the dipole theory of forces (DTF) for such particles.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2022)
Article
Optics
S. Orlov, J. Berskys
Summary: Research shows that photonic wheels can propagate laterally, unlike the usual longitudinal light waves. By utilizing a non-aplanatic system, we created photonic wheels and further applied them to the extension of vector spherical harmonics. We also found the appearance of localized longitudinal angular momentum in the scattered field when photonic wheels interact with gold and silicon spheres.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2021)
Article
Optics
Leonardo A. Ambrosio
Summary: The study examines a more general class of on-axis axisymmetric beams of the first kind within the theory of photophoresis, utilizing the generalized Lorenz-Mie theory (GLMT) for the calculation of asymmetry factors. The research demonstrates that the theory presented is valid for arbitrary size parameters and serves as a first attempt towards incorporating real optical wave fields into photophoresis of absorbing particles using the GLMT for arbitrary-shaped beams.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2021)
Article
Optics
Leonardo A. Ambrosio, Gerard Gouesbet
Summary: This work analytically demonstrates that the dipole theory for longitudinal forces is in complete agreement with the Rayleigh limit of the generalized Lorenz-Mie theory. It is observed that only a few poles actually contribute to the force exerted on the scatterer.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2021)
Article
Optics
Gerard Gouesbet, V. S. De Angelis, Leonardo Andre Ambrosio
Summary: This study extends the previous research on optical forces exerted on Rayleigh particles to the case of small magnetodielectric particles. It introduces a partition between gradient, scattering, and non-standard forces and emphasizes the relationship between these non-standard forces and curl forces in the framework of a dipole theory.
JOURNAL OF QUANTITATIVE SPECTROSCOPY & RADIATIVE TRANSFER
(2022)
Article
Mechanics
S. M. Abo-Dahab, Nahed S. Hussein, A. M. Abd-Alla, H. A. Alshehri
Summary: This article investigates the generalized magneto-thermoelasticity in an orthotropic non-homogeneous continuum solid with a spherical cavity. The solution for displacement, stress components, and temperature has been obtained using an integral transform technique. Comparisons have been made to verify the accuracy of the model, and the effects of inhomogeneity, relaxation time, and magnetic field on temperature, stresses, and displacement are analyzed and presented through graphs.
MECHANICS BASED DESIGN OF STRUCTURES AND MACHINES
(2022)
Article
Biophysics
Marketa Kubankova, Bettina Hohberger, Jakob Hoffmanns, Julia Fuerst, Martin Herrmann, Jochen Guck, Martin Kraeter
Summary: Various physical parameters of blood cells, including lymphocyte stiffness, monocyte size, neutrophil size and deformability, and heterogeneity of erythrocyte deformation and size, were found to change significantly in COVID-19 patients. While some of these changes recovered after hospitalization, others persisted for months after discharge, demonstrating the long-term impact of COVID-19 on the body.
BIOPHYSICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Yan Ge, Philipp Rosendahl, Claudio Duran, Nicole Toepfner, Sara Ciucci, Jochen Guck, Carlo Vittorio Cannistraci
Summary: Despite the widespread use of fluorescent cell labelling in biomedical studies, its drawbacks are inevitable, leading to a strong demand for label-free cell classification methods. Our research has shown promising results in label-free classification of different cells, contributing to the development of high-throughput morpho-rheological-based methodologies.
IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS
(2021)
Article
Biotechnology & Applied Microbiology
Gonzalo Rosso, Daniel Wehner, Christine Schweitzer, Stephanie Moellmert, Elisabeth Sock, Jochen Guck, Victor Shahin
Summary: Peripheral nerve injuries trigger a reprogramming of mature Schwann cells (SCs) into a repair phenotype that promotes nerve regeneration. SCs exhibit mechanosensitivity to the stiffness of the extracellular matrix, which affects gene expression and neurite outgrowth. The manipulation of SC substrate mechanosensing and neural plasticity may offer potential for designing bioengineered scaffolds that enhance nerve regeneration after injury.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Biophysics
Kyoohyun Kim, Vamshidhar R. Gade, Teymuras Kurzchalia, Jochen Guck
Summary: Optical diffraction tomography was used to assess the internal mass density distribution of Caenorhabditis elegans larvae, revealing a dramatic transition in the properties of dauer larvae upon harsh desiccation.
BIOPHYSICAL JOURNAL
(2022)
Letter
Biochemical Research Methods
Maik Herbig, Akihiro Isozaki, Dino Di Carlo, Jochen Guck, Nao Nitta, Robert Damoiseaux, Shogo Kamikawaji, Eigo Suyama, Hirofumi Shintaku, Angela Ruohao Wu, Itoshi Nikaido, Keisuke Goda
Article
Psychiatry
Andreas Walther, Anne Mackens-Kiani, Julian Eder, Maik Herbig, Christoph Herold, Clemens Kirschbaum, Jochen Guck, Lucas Daniel Wittwer, Katja Beesdo-Baum, Martin Kraeter
Summary: Depressive disorders, particularly persistent depressive disorders, are associated with increased deformability of blood cells, indicating mechanical changes in immune cells that may be predictive of persistent immune response.
TRANSLATIONAL PSYCHIATRY
(2022)
Article
Chemistry, Multidisciplinary
Marcus Inyama Asuquo, Emmanuel Effa, Oluwabukola Gbotosho, Akaninyene Otu, Nicole Toepfner, Soter Ameh, Sruti-Prathivadhi Bhayankaram, Noah Zetocha, Chisom Nwakama, William Egbe, Jochen Guck, Andrew Ekpenyong
Summary: This study utilized microfluidic technology to visualize and quantify the morphological changes in the blood of patients with sickle cell anemia. The results demonstrated the feasibility of personalized monitoring of patients' blood morphology for individualized treatment of sickle cell anemia complications.
APPLIED SCIENCES-BASEL
(2022)
Article
Multidisciplinary Sciences
Shada Abuhattum, Dominic Mokbel, Paul Mueller, Despina Soteriou, Jochen Guck, Sebastian Aland
Summary: Atomic force microscopy (AFM) is widely used for quantifying the mechanical properties of soft materials such as cells. Traditional methods using elastic parameters are insufficient to fully describe the mechanical properties of cells, whereas our research extends the understanding of cellular viscoelastic behavior by analyzing force-indentation curves.
Article
Biochemistry & Molecular Biology
Camilo Riquelme-Guzman, Timon Beck, Sandra Edwards-Jorquera, Raimund Schluessler, Paul Mueller, Jochen Guck, Stephanie Moellmert, Tatiana Sandoval-Guzman
Summary: In this work, the non-invasive and label-free confocal Brillouin microscopy technique was used to probe the mechanical properties in axolotls during development and regeneration. The results showed a gradual increase in cartilage Brillouin frequency shift, which was correlated with the regeneration stage. This study demonstrates the potential of Brillouin microscopy to investigate mechanical changes in vivo, laying the foundation for its application in the field of epimorphic regeneration.
Article
Biochemistry & Molecular Biology
Yohan Walter, Anne Hubbard, Allie Benoit, Erika Jank, Olivia Salas, Destiny Jordan, Andrew Ekpenyong
Summary: This study develops in vitro assays for the rapid evaluation of combined immunotherapy and radiotherapy strategies. The research finds that irradiated glioblastoma cells exhibit significantly increased migration in the first 20-40 hours post-treatment. Addition of the chemotherapy drug temozolomide increases cell migration rates, while the immune checkpoint inhibitor durvalumab abolishes the enhanced migration effect.
Article
Biochemical Research Methods
Ahmad Ahsan Nawaz, Despina Soteriou, Catherine K. Xu, Ruchi Goswami, Maik Herbig, Jochen Guck, Salvatore Girardo
Summary: Cell sorting based on mechanical properties has the potential to revolutionize the isolation of cells from heterogeneous populations. However, current methods sacrifice sorting rate for purity. We present a label-free cell sorting method using focused travelling surface acoustic wave in combination with real-time deformability cytometry, which enables high-throughput and high purity sorting of cells with different physical parameters. Our system also allows sorting of small cell fractions from concentrated populations, demonstrating the flexibility and applicability of the method.
Article
Biology
Spencer Mckinley, Adam Taylor, Conner Peeples, Megha Jacob, Gargee Khaparde, Yohan Walter, Andrew Ekpenyong
Summary: Unlike plants, animals do not have special gravity-sensing cells. However, microgravity can cause dysfunctions in bones, muscles, and immune systems in astronauts. Studies have shown changes at the cellular level induced by microgravity. It is important to understand the impact of microgravity on cellular drug response, considering the need for space medicine.
Article
Chemistry, Physical
Lucas Daniel Wittwer, Felix Reichel, Paul Mueller, Jochen Guck, Sebastian Aland
Summary: Real-time deformability cytometry (RT-DC) is a method that quantifies cell features on a single-cell level in real-time, and here we present two new lookup tables for RT-DC based on a neo-Hookean hyperelastic material obtained through simulations. The influence of the shear-thinning behavior of the surrounding medium on cell deformation in RT-DC is also analyzed, and insights about cell strain and stresses are provided. The new lookup tables and numerical cell shapes are freely available for use.
Article
Chemistry, Physical
Beyza Bueyuekurganci, Santanu Kumar Basu, Markus Neuner, Jochen Guck, Andreas Wierschem, Felix Reichel
Summary: In this study, a full shear-rheological description of methyl cellulose (MC) solutions was provided at different shear rates. The results showed that MC solutions exhibited power-law viscosity behavior and showed first normal stress difference at high shear rates. Furthermore, the impact of MC concentration on the rheology of the solutions was investigated, and the entanglement concentration was found to be approximately 0.64%. These findings contribute to a better understanding of the viscoelastic behavior of MC solutions and can be considered when modeling stress in microfluidic channels.
Article
Materials Science, Multidisciplinary
Nicolas Hauck, Timon Beck, Gheorghe Cojoc, Raimund Schlussler, Saeed Ahmed, Ivan Raguzin, Martin Mayer, Jonas Schubert, Paul Muller, Jochen Guck, Julian Thiele
Summary: Stretching individual living cells with light is a common method for evaluating their mechanical properties. However, the heat generated by the laser light used in optical stretchers can unintentionally alter the cells' mechanical properties. To address this issue, researchers have developed cell-sized elastic poly(N-isopropylacrylamide) (PNIPAAm) microgels that can indicate temperature changes through hydrogel swelling. By analyzing the effects of different gelation methods on the microgel network heterogeneity, micromechanics, and temperature response, the researchers have enhanced the use of standardized microgels for calibrating and validating cell mechanics analysis.
MATERIALS ADVANCES
(2022)
