Article
Biotechnology & Applied Microbiology
Abida Zahirovic, Ales Berlec
Summary: In this study, lactococcus lactis with potent and selective IL-6 binding activity was developed by displaying an IL-6-specific affibody on its surface. The engineered bacteria were able to efficiently remove IL-6 from solution and cell culture supernatant in a concentration-dependent manner. The results suggest that neutralizing IL-6 using this engineered strain may have important physiological significance for alleviating inflammatory bowel disease.
MICROBIAL CELL FACTORIES
(2022)
Article
Biology
Valerie Laroute, Catherine Beaufrand, Pedro Gomes, Sebastien Nouaille, Valerie Tondereau, Marie-Line Daveran-Mingot, Vassilia Theodorou, Helene Eutamene, Muriel Mercier-Bonin, Muriel Cocaign-Bousquet
Summary: This study found that the Lactococcus lactis NCDO2118 strain produces gamma-aminobutyric acid (GABA) in the gastrointestinal tract, which helps alleviate visceral hypersensitivity induced by stress. This discovery opens up new possibilities for using this GRAS bacterium to manage visceral pain and anxiety in patients with irritable bowel syndrome.
Article
Nanoscience & Nanotechnology
Fangfang Cao, Lulu Jin, Yong Gao, Yuan Ding, Hongyang Wen, Zhefeng Qian, Chenyin Zhang, Liangjie Hong, Huang Yang, Jiaojiao Zhang, Zongrui Tong, Weilin Wang, Xiaoyuan Chen, Zhengwei Mao
Summary: Combining artificial enzymes with probiotics, modified probiotics are used to treat inflammatory bowel disease, overcoming limitations of traditional treatments and showing promising outcomes.
NATURE NANOTECHNOLOGY
(2023)
Article
Microbiology
Camila Prosperi de Castro, Bianca Mendes Souza, Pamela Mancha-Agresti, Vanessa Bastos Pereira, Meritxell Zurita-Turk, Tatiane Melo Preisser, Vanessa Pecini da Cunha, Janete Soares Coelho dos Santos, Sophie Yvette Leclercq, Vasco Azevedo, Anderson Miyoshi
Summary: The development of a new tuberculosis vaccine strategy utilizing a lactococcal DNA vaccine fusion of ESAT-6 and Ag85A has shown promising results in mouse immunization studies, inducing significant cellular and humoral immune responses.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Chunyan Tan, Qiuyue Li, Xuejiao Yang, Jiayu Chen, Qilin Zhang, Xianyu Deng
Summary: Lactococcus lactis KUST48 (LLK48) with antibacterial effect against Streptococcus agalactiae was isolated and used as a potential probiotic to treat zebrafish infected with S. agalactiae. The study demonstrated the positive therapeutic effects of LLK48 on the intestinal microbiota and physiological functions of zebrafish infected with S. agalactiae, providing a new approach to treat S. agalactiae infections in fish aquaculture.
MICROBIOLOGY SPECTRUM
(2022)
Review
Biochemistry & Molecular Biology
Sofia O. D. Duarte, Gabriel A. Monteiro
Summary: The bacterium Lactococcus lactis, traditionally associated with the fermented food industry, is now being utilized in the pharmaceutical industry as a cell factory for the production of recombinant proteins and pDNA. Studies have shown its potential in live mucosal vaccination, with cell wall anchored antigens showing promising results. The critical review of alternative replicons for DNA vaccine carrier or antigen producer in live mucosal vaccination provides a starting platform for choosing the best vector for each application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Ghorban Ali Mahghani, Mohammad Kargar, Ezzat Allah Ghaemi, Farshid Kafilzadeh, Homa Davoodi
Summary: The esat-6 gene is conserved in both Beijing and non-Beijing isolates of M.tuberculosis. Higher expression of the esat-6 gene in Beijing isolates in the macrophage model indicates a potential role in increasing the pathogenicity of Beijing strains.
MICROBIAL PATHOGENESIS
(2022)
Article
Biotechnology & Applied Microbiology
Yue Chen, Lei Qiao, Xiaofan Song, Li Ma, Xina Dou, Chunlan Xu
Summary: This study demonstrated that L. lactis NZ9000-SeNPs effectively alleviated ETEC K88-induced intestinal barrier dysfunction in C57BL/6 mice by increasing villus height, reducing inflammatory cytokines, enhancing antioxidant enzyme activities, and modulating tight junction protein expression levels. These results suggest that L. lactis NZ9000-SeNPs could serve as a promising and safe selenium supplement for food or feed additives.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Nutrition & Dietetics
Dongming Yu, Yaoyao Xia, Liangpeng Ge, Bie Tan, Shuai Chen
Summary: Studies have shown that Lactococcus lactis can improve growth performance and regulate amino acid profiles, intestinal immunity, and microbiota in weaning piglets.
FRONTIERS IN NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Pedro Gomes, Valerie Laroute, Catherine Beaufrand, Marie-Line Daveran-Mingot, Nathalie Aubry, Chloe Liebgott, Nathalie Ballet, Sophie Legrain-Raspaud, Vassilia Theodorou, Muriel Mercier-Bonin, Muriel Cocaign-Bousquet, Helene Eutamene
Summary: Irritable bowel syndrome is a gastrointestinal disorder characterized by visceral hypersensitivity, which is exacerbated by stress. Gut bacteria that produce gamma-amino butyric acid (GABA) show promise in alleviating IBS symptoms. This study identified a strain of lactococcus lactis, CNCM I-5388, with increased GABA production rate and demonstrated its potential as a therapeutic agent for managing visceral hypersensitivity in IBS patients.
Article
Biotechnology & Applied Microbiology
Rocco Mazzolini, Irene Rodriguez-Arce, Laia Fernandez-Barat, Carlos Pinero-Lambea, Victoria Garrido, Agustin Rebollada-Merino, Anna Motos, Antoni Torres, Maria Jesus Grillo, Luis Serrano, Maria Lluch-Senar
Summary: Engineered bacteria have been developed to effectively treat lung infections and dissolve biofilms associated with ventilator-associated pneumonia. These bacteria have been genetically modified to target and kill harmful bacteria in the lungs, providing a potential new treatment option for respiratory infections.
NATURE BIOTECHNOLOGY
(2023)
Article
Virology
Lu Dai, Bock-Gie Jung, Jungang Chen, Buka Samten, James Craig Forrest, Steven R. Post, Zhiqiang Qin
Summary: KSHV-associated cancers are common in immunocompromised patients, while tuberculosis remains one of the deadliest infectious diseases globally; people living with HIV are at significantly higher risk of developing tuberculosis compared to those without HIV infection; the pathogenic factor ESAT-6 from Mtb can influence the biology of KSHV-infected cells.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Keila Lopes Mendes, Deborah de Farias Lelis, Daniela Fernanda de Freitas, Luiz Henrique da Silveira, Alfredo Mauricio Batista de Paula, Andre Luiz Sena Guimaraes, Janaina Ribeiro Oliveira, Marileia Chaves Andrade, Sergio Avelino Mota Nobre, Sergio Henrique Sousa Santos
Summary: The study demonstrated that the combination of resveratrol and Lactococcus lactis can reduce body weight, improve lipid metabolism, and decrease the expression of inflammatory markers, showing potential in preventing the development of liver diseases.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Fisheries
Junli Wang, Junchang Feng, Shasha Liu, Zhongliang Cai, Dongying Song, Liping Yang, Guoxing Nie
Summary: The study found that different forms of Lactococcus lactis Z-2 have significant effects on immune regulation, antioxidant properties, and promotion of nutrient metabolism in common carp, enhancing disease resistance and providing a theoretical basis for the rational use of potential probiotics.
Article
Biotechnology & Applied Microbiology
Tina Vida Plavec, Tim Kljucevsek, Ales Berlec
Summary: Researchers have successfully developed a modified BglBrick system in Lactococcus lactis, enabling simple and modular construction of multigene plasmids and controlled simultaneous expression of three proteins.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Immunology
Carine Rodrigues Pereira, Thiago de Jesus Sousa, Alessandra Lima da Silva, Roselane Goncalves dos Santos, Silvia Minharro, Dirceia Aparecida Costa Custodio, Derek J. Pickard, David O'Callaghan, Jeffrey T. Foster, Siomar de Castro Soares, Rommel Thiago Juca Ramos, Aristoteles Goes-Neto, Mateus Matiuzzi da Costa, Andrey Pereira Lage, Vasco Azevedo, Elaine Maria Seles Dorneles
Summary: This study reports the first isolation, identification, and characterization of Pseudochrobactrum saccharolyticum (strain 115) from Latin America. The strain was classified as P. saccharolyticum based on the analysis of its whole-genome sequence and 16S rRNA and recA gene sequences. This provides important insights into the diagnosis of bovine brucellosis and highlights the value of including the sequencing of these genes in routine diagnostics.
MICROBES AND INFECTION
(2023)
Article
Peripheral Vascular Disease
Beatriz G. S. Rocha, Caroline C. Picoli, Bryan O. P. Goncalves, Walison N. Silva, Alinne C. Costa, Michele M. Moraes, Pedro A. C. Costa, Gabryella S. P. Santos, Milla R. Almeida, Luciana M. Silva, Youvika Singh, Marcelo Falchetti, Gabriela D. A. Guardia, Pedro P. G. Guimaraes, Remo C. Russo, Rodrigo R. Resende, Mauro C. X. Pinto, Jaime H. Amorim, Vasco A. C. Azevedo, Alexandre Kanashiro, Helder I. Nakaya, Edroaldo L. Rocha, Pedro A. F. Galante, Akiva Mintz, Paul S. Frenette, Alexander Birbrair
Summary: This study reveals the presence of perivascular GFAP+/Plp1+ cells derived from tissue-resident Schwann cells in the tumor microenvironment. Genetic ablation of Schwann cells slows down tumor growth and angiogenesis, while boosting immune surveillance by increasing tumor-infiltrating anti-tumor lymphocytes and reducing immune-suppressor cells. In humans, increased expression of Schwann cell-related genes within melanoma is associated with improved survival.
Article
Cell Biology
Kenneth Lundstrom, Altijana Hromic-Jahjefendic, Esma Bilajac, Alaa A. A. Aljabali, Katarina Baralic, Nagwa A. Sabri, Eslam M. Shehata, Mohamed Raslan, Ana Claudia B. H. Ferreira, Lidiane Orlandi, Angel Serrano-Aroca, Murtaza M. Tambuwala, Vladimir N. Uversky, Vasco Azevedo, Khalid J. Alzahrani, Khalaf F. Alsharifm, Ibrahim F. Halawani, Fuad M. Alzahrani, Elrashdy M. Redwan, Debmalya Barhl
Summary: This review summarizes major events of SARS-CoV-2 infection route, immune response following host-cell infection as an important factor for disease outcome, as well as comorbidity issues of various tissues and organs arising due to COVID-19. Investigations on alterations of host-cell machinery and viral interactions with multiple intracellular signaling pathways could represent a major factor in more effective disease management.
CELLULAR SIGNALLING
(2023)
Article
Cell Biology
Debmalya Barh, Sandeep Tiwari, Lucas Gabriel Rodrigues Gomes, Cecilia Horta Ramalho Pinto, Bruno Silva Andrade, Shaban Ahmad, Alaa A. A. Aljabali, Khalid J. Alzahrani, Hamsa Jameel Banjer, Sk Sarif Hassan, Elrashdy M. Redwan, Khalid Raza, Aristoteles Goes-Neto, Robinson Sabino-Silva, Kenneth Lundstrom, Vladimir N. Uversky, Vasco Azevedo, Murtaza M. Tambuwala
Summary: The Omicron variant of SARS-CoV-2 is highly transmissible with decreased disease severity. It has lower pathogenicity but higher antigenicity compared to other variants. These findings contribute to our understanding of variant dynamics and can help in managing emerging variants.
Article
Microbiology
Pedro Negri Bernardino, Carolina Lechinski de Paula, Ana Flavia Marques Pereira, Marcio Garcia Ribeiro, Vasco Ariston de Carvalho Azevedo, Alexandre Secorun Borges, Ary Fernandes Jr, Jose Paes Oliveira-Filho
Summary: Caseous lymphadenitis is a disease caused by Corynebacterium pseudotuberculosis with minimal zoonotic potential. This study investigated the antimicrobial action of 2',3'-dideoxiadenosine (ddATP) against C. pseudotuberculosis and its potential synergy with penicillin, suggesting the potential of nucleotide analogs and combination therapy as a novel treatment for C. pseudotuberculosis infections.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Cell Biology
Mauro A. F. Guimaraes, Natalia Pinheiro-Rosa, Rafael P. Oliveira, Sarah L. F. Aguiar, Mariana C. G. Miranda, Luisa Lemos, Adna L. Souza, Daniela S. dos Reis, Samara R. Medeiros, William A. Goncalves, Sylvia S. Amaral, Vanessa Pinho, Anderson Miyoshi, Vasco A. Z. Azevedo, Rafael M. Rezende, Ana M. C. Faria
Summary: Multiple sclerosis (MS) is an autoimmune disease that affects the central nervous system. In this study, a protocol of oral tolerance induction using HSP65-producing Lactococcus lactis was proposed and successfully prevented and controlled MOG-induced experimental autoimmune encephalomyelitis (EAE) in mice. The treatment resulted in a reduction in EAE clinical score, higher frequencies of regulatory T cells, and reduced leukocyte adherence in the spinal cord. These findings suggest a novel therapeutic strategy for MS and other autoimmune diseases.
CELLULAR IMMUNOLOGY
(2023)
Article
Ecology
Priscyla dos Santos Ribeiro, Natalia Barbosa Carvalho, Flavia Aburjaile, Thiago Sousa, Graciete Verissimo, Talita Gomes, Fabio Neves, Luiza Blanco, Joao Antonio Lima, Daiana de Oliveira, Arun Kumar Jaiswal, Bertram Brenig, Siomar Soares, Rommel Ramos, Mateus Matiuzzi, Aristoteles Goes-Neto, Claudio Pereira Figueira, Federico Costa, Paula Ristow, Vasco Azevedo
Summary: This study identified a new non-pathogenic species of spirochetes in biofilms in a polluted urban environment, suggesting that biofilms may serve as natural reservoirs for leptospires.
Review
Biochemistry & Molecular Biology
Emilio Mateev, Maya Georgieva, Alexandrina Mateeva, Alexander Zlatkov, Shaban Ahmad, Khalid Raza, Vasco Azevedo, Debmalya Barh
Summary: With the increase in neurodegenerative diseases, monoamine oxidase type B (MAO-B) inhibitors are emerging as promising structures for treatment. Structure-based virtual screening (SBVS), specifically using molecular docking, is a useful tool for drug discovery and development. This study provides insight into the role of MAOs in treating neurodegenerative diseases, discusses the advantages and disadvantages of docking simulations and software, and explores the active sites of MAO-A and MAO-B. New chemical classes of MAO-B inhibitors and their essential fragments are also discussed, providing potential directions for further research.
Article
Immunology
Muhammad Usman, Aaima Ayub, Sabahat Habib, Muhammad Suleman Rana, Zaira Rehman, Ali Zohaib, Syed Babar Jamal, Arun Kumar Jaiswal, Bruno Silva Andrade, Vasco de Carvalho Azevedo, Muhammad Faheem, Aneela Javed
Summary: In this study, a multivalent vaccine was designed using VP4 and VP7 proteins of Rotavirus A. Epitopes with antigenicity, non-allergenicity, homology with humans, and anti-inflammatory properties were screened to construct the vaccine. Immune simulation showed promising results in both Asia and worldwide. However, in-vitro/in-vivo screening is necessary for a definitive conclusion.
Article
Microbiology
Joyce Dutra, Glen Garcia, Rosimeire Gomes, Mariana Cardoso, Arley Cortes, Tales Silva, Luis de Jesus, Luciano Rodrigues, Andria Freitas, Vinicius Waldow, Juliana Laguna, Gabriela Campos, Monique Americo, Rubens Akamine, Maira de Sousa, Claudia Groposo, Henrique Figueiredo, Vasco Azevedo, Aristoteles Goes-Neto
Summary: The study aimed to analyze the physicochemical features of microbial communities occurring in produced water and enrichment cultures in oil pipelines. It was found that produced water samples have higher phylogenetic diversity of bacteria and archaea, while enrichment cultures have higher dominance of bacterial genera associated with microbial influenced corrosion (MIC). All samples had a core community composed of 19 distinct genera, with Desulfovibrio as the dominant genus. Metabarcoding of uncultured produced water samples, along with physicochemical characterization, was suggested as a more efficient method for monitoring MIC in oil industry facilities.
Article
Microbiology
Juan Carlos Ariute, Andrei Giachetto Felice, Siomar Soares, Marco Aurelio Siqueira da Gama, Elineide Barbosa de Souza, Vasco Azevedo, Bertram Brenig, Flavia Aburjaile, Ana Maria Benko-Iseppon
Summary: In this study, 14 Ralstonia solanacearum isolates from Brazil were sequenced and analyzed, revealing their similarity to R. solanacearum genomes in NCBI. The new isolates shared more Rips with Moko IIA and Moko IIB than with other public BW genome isolates from Brazil. These findings provide important insights into the association between Rips repertoire and host specificities.
Article
Infectious Diseases
Jordana Almeida Santana, Amanda Oliveira Paraguassu, Ranielle Stephanie Toledo Santana, Rafael Gariglio Clark Xavier, Patricia Maria Colleto Freitas, Flavia Figueira Aburjaile, Vasco Ariston de Carvalho Azevedo, Bertram Brenig, Anders Miki Bojesen, Rodrigo Otavio Silveira Silva
Summary: This study evaluated the risk factors associated with MRSP colonization in dogs admitted to an ICU, and reported antimicrobial resistance profiles and genetic relatedness of MRSP isolates. The results found that female sex, age (>7 years), and previous antimicrobial treatment were associated with MRSP acquisition. Whole-genome sequencing revealed the presence of the same MRSP clone causing infections in dogs outside the ICU. Therefore, improved infection prevention and control practices are necessary in veterinary hospitals, especially in ICUs.
Article
Infectious Diseases
Jose Cleves da Silva Maia, Gabriel Amorim de Albuquerque Silva, Leticia Stheffany de Barros Cunha, Gisele Veneroni Gouveia, Aristoteles Goes-Neto, Bertram Brenig, Fabricio Almeida Araujo, Flavia Aburjaile, Rommel Thiago Juca Ramos, Siomar Castro Soares, Vasco Ariston de Carvalho Azevedo, Mateus Matiuzzi da Costa, Joao Jose de Simoni Gouveia
Summary: This study sequenced and compared Aeromonas veronii isolates to reveal misidentification of genomes and identify a significant number of virulence and antibiotic resistance genes. The results highlight the importance of using methods like ANI to correctly classify genomes, and indicate that A. veronii from local isolates possess similar virulence characteristics as those in public databases, with widespread colistin resistance genes requiring greater attention for surveillance systems.
Article
Genetics & Heredity
Carine Rodrigues Pereira, Rodrigo Bentes Kato, Fabricio Almeida Araujo, Alessandra Lima da Silva, Roselane Goncalves dos Santos, Thiago de Jesus Sousa, Raquel Costa Neia, Saulo Britto da Silva, Charles H. D. Williamson, John Gillece, Andrey Pereira Lage, David O'Callaghan, Derek Pickard, Rommel Thiago Juca Ramos, Vasco Ariston de Carvalho Azevedo, Jeffrey T. Foster, Elaine Maria Seles Dorneles
Summary: This study investigated the genetic determinants responsible for antimicrobial resistance in 53 genomes of B. abortus strains isolated from cattle in Brazil. Eight genes associated with antimicrobial resistance were identified, but no association was found between the genetic polymorphisms in these genes and the observed resistance phenotypes in the Brazilian strains.
Article
Immunology
Maisa Santos Fonseca, Kathleen Ramos Deegan, Luiz Marcelo Tome, Marcos Antonio Mendonca, Ana Rita Sokolonski, Leane Queiroz Gondim, Vasco Azevedo, Roberto Meyer, Ljubica Tasic, Aristoteles Goes-Neto, Ricardo Wagner Portela
Summary: This study examines a novel pathogen, Candida haemulonii, causing cutaneous infection in a Boa constrictor snake. The fungus was identified and its drug sensitivity was tested, revealing strong inhibition for all drugs except fluconazole and itraconazole. A biogenic silver nanoparticle-based ointment successfully treated the clinical symptoms in the snake. The presence of these infections in snakes near human habitats highlights the importance of wildlife health monitoring for emerging and opportunistic diseases in peri-urban environments.
MICROBIAL PATHOGENESIS
(2023)