Article
Physics, Applied
E. Alfinito, R. Cataldo, L. Reggiani
Summary: Biological matter is gaining attention for its innovative features and applications in technology. This study explores the use of a photosensitive protein to build an electrical pH sensor, demonstrating the feasibility of the bio-rheostat hypothesis and supporting the development of electron devices based on biomaterials.
APPLIED PHYSICS LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Xiao-Lin Liu, Ming-Qing Chen, Yang-Lin Jiang, Rong-Yao Gao, Ze-Jun Wang, Peng Wang
Summary: The purple non-sulfur bacterium Rhodobacter sphaeroides was used as a model to study its response to the toxicity of different alkyl chain lengths of 1-alkyl-3-methylimidazolium bromide ([C(n)mim]Br), an ionic liquid. The inhibition of bacterial growth was positively correlated with the length of the alkyl chain. The ILs caused cell membrane perforation and affected the absorption bands and enzyme activity of the bacterium.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Multidisciplinary Sciences
Richard McLean, Thomas Schwander, Christoph Diehl, Nina Socorro Cortina, Nicole Paczia, Jan Zarzycki, Tobias J. Erb
Summary: Nature has developed eight different pathways to capture and convert CO2, but these pathways have limitations and only represent a fraction of the potential solutions. To overcome these limitations, scientists have designed a new CO2-fixation pathway called the HydrOxyPropionyl-CoA/Acrylyl-CoA (HOPAC) cycle. Through step-wise implementation and optimization using engineering approaches and machine learning, the HOPAC cycle can convert approximately 3.0 mM of CO2 into glycolate within 2 hours. This research provides a foundation for potential applications of the HOPAC cycle.
Article
Biotechnology & Applied Microbiology
Tong Shi, Lu Zhang, Mindong Liang, Weishan Wang, Kefeng Wang, Yue Jiang, Jing Liu, Xinwei He, Zhiheng Yang, Haihong Chen, Chuan Li, Dongyuan Lv, Liming Zhou, Biqin Chen, Dan Li, Li-Xin Zhang, Gao-Yi Tan
Summary: In this study, several native promoters from Rhodobacter sphaeroides JDW-710 were characterized, with two showing significantly higher activity than the commonly used tac promoter. A synthetic promoter library was also created, leading to successful modulation of the Q10 synthetic pathway in JDW-710 and increased production yields.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lea Vidal, Esteban Lebrun, Young-Kyoung Park, Guillaume Mottet, Jean-Marc Nicaud
Summary: In this study, novel bidirectional and hybrid bidirectional promoters were developed, whose expression can be fine-tuned to meet the demand for versatile promoters in the yeast Y. lipolytica. This study provides effective tools for smoothly adjusting the erythritol-inducible co-expression of two target genes in biotechnology applications. BDPs developed in this study have potential applications in the fields of heterologous protein production, metabolic engineering, and synthetic biology.
MICROBIAL CELL FACTORIES
(2023)
Article
Plant Sciences
Ramona Persad-Russell, Mitra Mazarei, Tayler Marie Schimel, Lana Howe, Manuel J. Schmid, Tayebeh Kakeshpour, Caitlin N. Barnes, Holly Brabazon, Erin M. Seaberry, D. Nikki Reuter, Scott C. Lenaghan, C. Neal Stewart
Summary: The study demonstrated the amplification of plant sensor signals using a synthetic transcription factor gene to achieve rapid detection of pathogenic bacterial infection, leading to successful identification of specific pathogens within the plant family.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Chandra Kanth Bandi, Kyle S. Skalenko, Ayushi Agrawal, Neelan Sivaneri, Margaux Thiry, Shishir P. S. Chundawat
Summary: The study developed an engineered synthetic promoter system called cyn regulon to selectively detect azide ions and induce downstream expression of reporter genes. The engineered cyn azide operon showed highly tunable expression over a range of azide ion concentrations and rapidly induced reporter gene expression.
ACS SYNTHETIC BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Enrico Orsi, Jules Beekwilder, Gerrit Eggink, Serve W. M. Kengen, Ruud A. Weusthuis
Summary: Microbial cell factories play a crucial role in industrial biotechnology, and microbial strain engineering through the DBTL concept has been successful in improving competitiveness. New-to-industry microorganisms like Rhodobacter sphaeroides are being investigated as novel cell factories, showcasing potential for bio-based product synthesis.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Alexander Klotz, Andreas Kaczmarczyk, Urs Jenal
Summary: Reverse genetics is a powerful approach to study bacterial physiology and behavior using genetic tools. However, well-characterized inducible promoters are scarce for the human pathogen Pseudomonas aeruginosa. In this study, a synthetic cumate-inducible promoter, called P-QJ, was developed for P. aeruginosa, which showed excellent induction properties at the single-cell level.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Fangting Wu, Ying Zhou, Wenyu Pei, Yuhan Jiang, Xiaohui Yan, Hong Wu
Summary: This study constructed an optimized pathway for PHB production using sucrose as the sole carbon source and guided gene knockout to reduce byproduct generation. The use of sucrose as a carbon source under anaerobic conditions significantly improved PHB yield and production costs.
Article
Biochemical Research Methods
Elena Moreno-Gimenez, Sara Selma, Camilo Calvache, Diego Orzaez
Summary: Programmable transcriptional factors based on CRISPR architecture have been widely used in plants for endogenous gene regulation. The dCasEV2.1 activation system, a powerful CRISPR tool for gene induction in plants, exhibits remarkable genome-wide specificity and strong activation capacity. By utilizing the GB_SynP collection of DNA parts, a total of 35 synthetic promoters were successfully assembled, showing predictable activation levels and minimal background. Furthermore, GB_SynP was employed to analyze the combinatorial expression of an autoluminescence pathway in Nicotiana benthamiana, demonstrating the valuable role of this tool in extracting important biological information.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Biology
Xiaofan Feng, Mario Andrea Marchisio
Summary: Promoters are DNA sequences that play a crucial role in initiating transcription. They can function constitutively or be regulated by different environmental signals. The strength of promoters significantly impacts the expression of proteins and RNA in yeast genetic circuits. Advances in promoter engineering have been driven by Synthetic Biology, with a focus on constructing artificial promoters for genetic circuits in popular chassis like S. cerevisiae.
Article
Biochemical Research Methods
Laszlo Mozsik, Carsten Pohl, Vera Meyer, Roel A. L. Bovenberg, Yvonne Nygard, Arnold J. M. Driessen
Summary: This paper presents a collection of 96 genetic parts that can be used in synthetic biology research in fungi, allowing for the assembly of complex multipartite components. It provides a standardized and modular approach for the rapid assembly of synthetic transcription units.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chen Chen, Jun Chen, Guangxi Wu, Liling Li, Zhangli Hu, Xiaozheng Li
Summary: In this study, a synthetic promoter GA was constructed by hybridizing core fragments from natural promoters, which successfully enhanced the expression level of exogenous genes and showed strong activity under blue light stimulation. This provides useful synthetic biology tools for algal biotechnology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biodiversity Conservation
Michelle Rourke
Summary: This article discusses the issues in the regulatory regime of access and benefit-sharing (ABS) in synthetic biology, particularly the impact of regulating digital sequence information (DSI) on bioparts and the stability of values. The author argues that if DSI is included in the current regulatory regime, the openness and sharing in synthetic biology may be affected.
PLANTS PEOPLE PLANET
(2022)