Article
Biotechnology & Applied Microbiology
Gabriele Cerutti, Elena Gugole, Linda Celeste Montemiglio, Annick Turbe-Doan, Dehbia Chena, David Navarro, Anne Lomascolo, Francois Piumi, Cecile Exertier, Ida Freda, Beatrice Vallone, Eric Record, Carmelinda Savino, Giuliano Sciara
Summary: A novel enzymatic activity, oligosaccharide dehydrogenase (ODH), was identified with a preference for oligosaccharides containing beta(1 -> 3) glycosidic bond. The crystallographic structures revealed a unique saccharide recognition mechanism and distinct stabilizing interactions, providing new insights into substrate binding and enzymatic functions within the Carbohydrate-Active enZymes database.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Food Science & Technology
Minyu Cai, Liyan Huang, Sashuang Dong, Nanxin Diao, Weilian Ye, Zhiye Peng, Xiang Fang
Summary: This study aimed to enhance the flavor of Summer green tea (SGT) by using fermentation with A. niger RAF106 and examining the changes in its metabolites during this process. The results showed that the content of alcohol, alkanes, and nitroxides in tea leaves increased after fermentation. The volatile compounds identified in fermented tea leaves included linalool, (Z)-alpha, alpha, 5-trimethyl-5-vinyltetrahydrofuran-2-methanol, (E)-linalool oxide (furan type), linalool oxide (pyran type), and theapyrrole, and their content significantly increased compared to the non-fermented control group. The study also found that the content of non-ester catechins, soluble sugars, and total flavonoids reached their peak on the 4th day of fermentation, while gallic acid and free amino acids reached their peak on the 6th day. However, the content of ester catechin and caffeine decreased during fermentation. The content of lactic acid, acetic acid, and citric acid in tea after fermentation was significantly higher compared to before fermentation. This study offers a new approach for improving the quality of SGT.
Article
Microbiology
Junwei Zheng, Linlin Yao, Xu Zeng, Bin Wang, Li Pan
Summary: This study identified and characterized all putative ER cargo receptors in Aspergillus niger, and compared the colony morphology and protein secretion status of each receptor strain. The deletion of Erv14 significantly inhibited mycelial growth and secretion of extracellular proteins. Erv14 was found to specifically interact with transporters and associated with the transport of proteins involved in cell wall synthesis, lipid metabolism, and organic substrate metabolism.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Claude Lambre, Jose Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Riviere, Inger-Lise Steffensen, Christina Tlustos, Henk van Loveren, Laurence Vernis, Holger Zorn, Boet Glandorf, Lieve Herman, Ana Gomes, Yi Liu, Joaquim Maia, Sandra Rainieri, Andrew Chesson
Summary: The food enzyme produced with genetically modified Aspergillus niger strain is considered safe for use in egg processing. Although there is a potential risk of allergic reactions, it is deemed to be low.
Article
Microbiology
Michel Flipphi, Alexandra Marton, Vivien Biro, Norbert Ag, Erzsebet Sandor, Erzsebet Fekete, Levente Karaffa
Summary: AOX is a terminal oxidase in the mitochondrial electron transport chain and plays a role in fungal diseases caused by black aspergilli. Variation in the aoxB gene enables the subdivision of A. niger strains into six taxa, facilitating the identification of individual species.
Article
Microbiology
Yunqi Zhu, Tong Liu, Yingsi Wang, Guojun Chen, Xiang Fang, Gang Zhou, Jie Wang
Summary: This study demonstrated the important functions of ChsA, a class II chitin synthase, in Aspergillus niger. The deletion of chsA resulted in severe defects in conidiation and mycelial morphology, as well as reduced growth under cell wall-disturbing and oxidative stresses. ChsA also played a role in regulating the production of malic acid, enzymes, and citric acid in A. niger.
Article
Environmental Sciences
Jay Kumar, Amanpreet Kaur Sodhi, Neeraj Bhanot
Summary: This study aims to utilize pre-treated induction furnace dust with Aspergillus niger fungus to replace cement in concrete construction. The best results were obtained with a dust replacement of 14.96%, a curing time of 28 days, and a cell count of 9.22 x 10(7) CFU/ml of distilled water for concrete production.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Food Science & Technology
Claude Lambre, Jose Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Riviere, Inger-Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Jaime Aguilera, Magdalena Andryszkiewicz, Yi Liu, Giulio di Piazza, Sandra Rainieri, Andrew Chesson
Summary: The food enzyme catalase is produced by a non-genetically modified strain of Aspergillus niger and is used in eight food manufacturing processes. Dietary exposure to the food enzyme was estimated to be up to 3.61 mg TOS/kg bw per day in European populations. Based on the data provided, the risk of exposure cannot be completely excluded.
Article
Chemistry, Medicinal
Dalia H. Abdelkader, Walaa A. Negm, Engy Elekhnawy, Duaa Eliwa, Basmah N. Aldosari, Alanood S. Almurshedi
Summary: In this study, zinc oxide nanoparticles (ZnO NPs) were synthesized using the endophytic fungal extract of Aspergillus niger, and their antibacterial activity was investigated in vitro and in vivo. The biosynthesized ZnO NPs showed promising antibacterial action against Staphylococcus aureus isolates, and exhibited protective effects on liver and spleen tissues in a systemic infection model in mice.
Article
Food Science & Technology
Claude Lambre, Jose Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Riviere, Inger-Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Jaime Aguilera, Magdalena Andryszkiewicz, Natalia Kovalkovicova, Yi Liu, Andrew Chesson
Summary: This article describes the safety assessment of a genetically modified Aspergillus niger strain producing the food enzyme peroxidase. The study found that the enzyme does not raise safety concerns under the intended conditions of use.
Article
Food Science & Technology
Claude Lambre, Jose Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Riviere, Inger-Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Boet Glandorf, Lieve Herman, Magdalena Andryszkiewicz, Ana Gomes, Yi Liu, Sandra Rainieri, Andrew Chesson
Summary: The genetically modified food enzyme glucose oxidase is deemed safe for use in baking, cereal-based processes, and egg processing. Toxicity tests and exposure assessments indicate that there are no safety concerns when used within the recommended limits. Although there is a potential risk of allergic reactions, the likelihood is considered to be low.
Article
Nanoscience & Nanotechnology
H. N. Hieu, D. T. H. Trang, V. T. T. Hien, N. Nghia, N. T. Lam, T. M. D. Nguyen
Summary: This study reports the biosynthesis of silver nanoparticles using Bacillus megaterium and Aspergillus niger. The results show that the characteristic UV-vis absorbance peak of AgNPs synthesized using Aspergillus niger was observed in the 433 - 448 nm range and around 475 nm with Bacillus megaterium. The AgNPs have potential antibacterial activity against Escherichia coli.
DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES
(2022)
Article
Food Science & Technology
Farahnaz Karami, Mohammad Ghorbani, Alireza Sadeghi Mahoonak, Dareuosh Shackebaei, Reza Khodarahmi
Summary: This study investigates the effect of A. niger beta-glucosidase on grapefruit peel extract. The results show that enzyme treatment significantly enhances the extract's antioxidant activity and promotes the production of alkyl glucosides. The produced extract can be used as a natural and low-cost antioxidant for food.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2022)
Article
Infectious Diseases
Pierre-Edouard Fournier, Philippe Colson, Anthony Levasseur, Christian A. Devaux, Philippe Gautret, Marielle Bedotto, Jeremy Delerce, Ludivine Brechard, Lucile Pinault, Jean-Christophe Lagier, Florence Fenollar, Didier Raoult
Summary: In Marseille, France, a second wave of SARS-CoV-2 epidemic occurred from June, with the Marseille-4 variant being the major cause, carrying 13 hallmark mutations including one affecting the receptor binding domain of the spike protein. This variant has caused 2106 infections since September 2020 and is associated with a higher proportion of hypoxemia.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Hacene Medkour, Sergei Castaneda, Inestin Amona, Florence Fenollar, Claudine Andre, Raphael Belais, Paulin Mungongo, Jean-Jacques Muyembe-Tamfum, Anthony Levasseur, Didier Raoult, Bernard Davoust, Oleg Mediannikov
Summary: The study screened bonobos in the Democratic Republic of the Congo for potential human infectious agents, finding a high prevalence of zoonotic viruses, bacteria, and parasites in their stool samples. The results suggest that bonobos may serve as reservoirs for certain human pathogens, highlighting the importance of studying disease transmission between humans and our closest living relatives.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Praneeth M. Mendis, Zachary J. Sasiene, David Ropartz, Helene Rogniaux, Glen P. Jackson
Summary: Ultra-high-performance liquid chromatography with charge transfer dissociation mass spectrometry was used to analyze a mixture of complex sulfated oligosaccharides, showing more abundant ions and less neutral losses compared to collision-induced dissociation. Optimization of conditions led to more confident structural detail, highlighting the compatibility of He-CTD with UHPLC and the enhanced structural information provided.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
David Ribeaucourt, Safwan Saker, David Navarro, Bastien Bissaro, Elodie Drula, Lydie Oliveira Correia, Mireille Haon, Sacha Grisel, Nicolas Lapalu, Bernard Henrissat, Richard J. O'Connell, Fanny Lambert, Mickael Lafond, Jean-Guy Berrin
Summary: The discovery of Copper radical alcohol oxidases (CRO-AlcOx) among fungal phytopathogens has opened up new possibilities for the production of fragrant fatty aldehydes. Through proteomics analysis, it was found that under specific conditions, Colletotrichum species could secrete a wide variety of copper-containing enzymes, but only small amounts of CRO-AlcOx were secreted, indicating that recombinant expression may be a more promising approach for their biocatalytic application.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Alvin Ho-Ting Li, Amit X. Garg, Jeremy M. Grimshaw, Versha Prakash, Alexie J. Dunnett, Stephanie N. Dixon, Monica Taljaard, Joanna Mitchell, Kyla L. Naylor, Cathy Faulds, Rachel Bevan, Leah Getchell, Greg Knoll, S. Joseph Kim, Jessica Sontrop, Allison Tong, Lise M. Bjerre, Karyn Hyjek, Donna Currie, Susan Edwards, Mike Sullivan, Linda Harvey-Rioux, Justin Presseau
Summary: This study aimed to evaluate the effectiveness of promoting organ donation registration in family physician offices. However, the intervention did not increase donor registration. Nonetheless, family physician offices may still have the potential to develop and evaluate better interventions to increase organ donation registration.
Article
Medicine, General & Internal
Anna J. Hussey, Robert S. McKelvie, Madonna Ferrone, Teresa To, Melissa Fisk, Dhssraj Singh, Cathy Faulds, Christopher Licskai
Summary: This study aims to evaluate the efficacy of integrated disease management (IDM) focused on optimizing medication, self-management, and structured follow-up in a high-risk primary care HF population. 100 family physician clusters will be recruited in this Canadian primary care multicentre cluster randomised controlled trial. The study has the power to detect a 35% reduction in the primary outcome, with findings to be disseminated through local reports, presentations, and peer-reviewed publications.
Article
Microbiology
Amal Ben Ayed, Bilel Hadrich, Giuliano Sciara, Anne Lomascolo, Emmanuel Bertrand, Craig B. Faulds, Hela Zouari-Mechichi, Eric Record, Tahar Mechichi
Summary: The textile industry produces a large amount of colored wastewater containing toxic and carcinogenic dyes, which require multiple treatments for removal. In this study, laccase-like active cell-free supernatant from Coriolopsis gallica was used for the decolorization of the recalcitrant azo dye Reactive Black 5. By optimizing the reaction conditions, a maximum decolorization rate of 82 +/- 0.6% was achieved in a 1 mL reaction mixture.
Article
Microbiology
Amal Ben Ayed, Imen Akrout, Quentin Albert, Stephane Greff, Charlotte Simmler, Jean Armengaud, Melodie Kielbasa, Annick Turbe-Doan, Delphine Chaduli, David Navarro, Emmanuel Bertrand, Craig B. Faulds, Mohamed Chamkha, Amina Maalej, Hela Zouari-Mechichi, Giuliano Sciara, Tahar Mechichi, Eric Record
Summary: The wastewater containing antibiotics from hospitals, pharmaceutical industries, and human and animal excretion poses a significant threat to the environment. This study investigated the ability of three fungal strains to degrade the fluoroquinolone antibiotic levofloxacin. The results showed that Coriolopsis gallica strain CLBE55 exhibited the highest removal efficiency, with a 15% decrease in antibiogram zone of inhibition for Escherichia coli in solid medium and a 25% degradation of the antibiotic in liquid medium. Proteomic analysis suggested the involvement of laccases and dye-decolorizing peroxidases in the degradation process, with laccases playing a major role. Mass spectrometry analysis confirmed the formation of an N-oxidized derivative as the main product of levofloxacin biotransformation by Coriolopsis gallica.
Article
Biotechnology & Applied Microbiology
Jean-Lou Reyre, Sacha Grisel, Mireille Haon, David Navarro, David Ropartz, Sophie Le Gall, Eric Record, Giuliano Sciara, Olivier Tranquet, Jean-Guy Berrin, Bastien Bissaro
Summary: Filamentous fungi, such as Ustilago maydis, play a key role in regulating various processes on Earth. They secrete carbohydrate-active enzymes (CAZymes) to modify and degrade carbohydrates. In this study, researchers analyzed the secretome of U. maydis and identified several CAZymes involved in the modification of the fungal cell wall components. They characterized two new CAZymes and found a potential biocatalytic cascade. These findings provide insights into the role of CAZymes in fungi and highlight the need for further research in this area.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sona Malric-Garajova, Florian Fortuna, Florian Pion, Elise Martin, Adithya Raveendran Thottathil, Audrey Guillemain, Annick Doan, Anne Lomascolo, Craig B. Faulds, Stephanie Baumberger, Laurence Foulon, Brigitte Chabbert, Helene de Baynast, Pascal Dubessay, Fabrice Audonnet, Emmanuel Bertrand, Giuliano Sciara, Sandra Tapin-Lingua, Paul-Henri Ducrot, Philippe Michaud, Veronique Aguie-Beghin, Eric Record
Summary: This study aims to improve the functionality of industrial lignin through enzymatic modification. Kraft lignin from marine pine was treated with high-redox-potential laccase from Pycnoporus cinnabarinus fungus at different concentrations and pH conditions, with or without the chemical mediator 1-hydroxybenzotriazole (HBT). The structural changes in lignin and the solvent-extractable fractions were analyzed using FTIR, DSC, HPSEC, and GC-MS. The results demonstrate that P. cinnabarinus laccase can effectively modify marine pine kraft lignin, and the analytical methods used in this study are valuable for screening enzymatic treatment conditions.
Article
Biotechnology & Applied Microbiology
Nian Liu, Severine Gagnot, Yann Denis, Deborah Byrne, Craig Faulds, Henri-Pierre Fierobe, Stephanie Perret
Summary: This study discovered a second system of arabinoxylan degradation in Ruminiclostridium cellulolyticum, challenging the previous assumption that complete extracellular degradation of arabinoxylan is required. The findings shed new light on the strategies used by anaerobic primary degrader bacteria to metabolize highly decorated arabinoxylan in competitive environments.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2022)
Article
Chemistry, Multidisciplinary
Eden Kaddouch, Maria E. Cleveland, David Navarro, Sacha Grisel, Mireille Haon, Harry Brumer, Mickael Lafond, Jean-Guy Berrin, Bastien Bissaro
Summary: This study presents a simple method for direct measurement of galactose oxidase (GalOx) activity by identifying galactosylated oxidized products using high-performance anion-exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD). The results showed that this method is more sensitive than other methods.
Article
Biotechnology & Applied Microbiology
Nicholas G. S. McGregor, Casper de Boer, Mikhaaeel Santos, Mireille Haon, David Navarro, Sybrin Schroder, Jean-Guy Berrin, Herman S. Overkleeft, Gideon J. Davies
Summary: This study demonstrates that ABP-Cel can be used to analyze endo-beta-glucanase secreted by different fungi, revealing significant dynamics and unique enzyme fingerprints associated with each species substrate combination. Chemical proteomic analysis identifies significant arsenals of cellulases secreted by each fungal species during growth on lignocellulosic biomass. Recombinant production and characterization of a collection of probe-reactive enzymes from GH5, GH10, and GH12 confirm that ABP-Cel shows broad selectivity towards enzymes with endo-beta-glucanase activity.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2022)