Article
Chemistry, Physical
Davide Rigo, Nadia Alessandra Carmo Dos Santos, Alvise Perosa, Maurizio Selva
Summary: An unprecedented two-step sequence was designed using batch and continuous flow (CF) protocols to upgrade two aminodiol regioisomers derived from glycerol. The substrates were quantitatively converted into amides under batch conditions, followed by a selective CF-acetalisation reaction to produce double-functionalized products.
Article
Agricultural Engineering
Xun Wang, Xinyi Zhang, Jia Zhang, Longjie Xiao, Yujunjie Zhou, Fei Wang, Xun Li
Summary: A powerful green cell factory has been developed to produce linalool from biodiesel-derived glycerol. Engineered Escherichia coli achieved a linalool titer of 1.07 g/L in flask culture, the highest reported in E. coli to date.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Chemistry, Multidisciplinary
Aleksandar R. Todorov, Alistair W. T. King, Ilkka Kilpeläinen
Summary: A sustainable homogeneous transesterification protocol using [mTBNH][OAc] and unactivated methyl esters has been developed to prepare cellulose esters with controllable degree of substitution. The superbase ionic liquid [mTBNH][OAc] can be recycled with high purity for repeated use, allowing not only activated or cyclic esters but also unactivated methyl esters for cellulose transesterification reactions, thus expanding the substrate and application scope. Furthermore, the solubility properties of the prepared cellulose materials were tested, revealing some intrinsic trends at low degrees of substitution.
Article
Biochemistry & Molecular Biology
Yihang Jing, Jose L. Montano, Michaella Levy, Jeffrey E. Lopez, Pei-Pei Kung, Paul Richardson, Krzysztof Krajewski, Laurence Florens, Michael P. Washburn, Jordan L. Meier
Summary: Chemical proteomics offers a powerful strategy for high-throughput assignment of enzyme function or inhibitor selectivity, but optimizing probes for specific enzyme family members and distinguishing signals from background noise remain challenging. This study introduces a physiochemical discernment strategy based on the CoA cofactor for improving chemical proteomics, demonstrating its efficacy in identifying optimized pulldown probes and comparing its selectivity against traditional competitive methods. The use of CoA-based chemoproteomic probes is validated in evaluating the specificity of small molecule acetyltransferase inhibitors, showcasing their potential in drug discovery.
ACS CHEMICAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Jian Lu, Xue Qi Li, Pei Pei Chen, Jia Xiu Zhang, Liang Liu, Gui Hua Wang, Xiao Qi Liu, Ting Ting Jiang, Meng Ying Wang, Wen Tao Liu, Xiong Zhong Ruan, Kun Ling Ma
Summary: This study found that ACSS2 activation promoted podocyte injury in diabetic nephropathy by inhibiting autophagy through the raptor/mTORC1 pathway. Inhibition of ACSS2 improved autophagy and alleviated podocyte injury.
Article
Biochemical Research Methods
David M. Hollenstein, Margarita Maurer-Granofszky, Wolfgang Reiter, Dorothea Anrather, Thomas Gossenreiter, Riccardo Babic, Natascha Hartl, Claudine Kraft, Markus Hartl
Summary: We present an effective method to reduce codigestion of bead-bound ligands in affinity purification-mass spectrometry experiments. The method utilizes nontoxic chemicals and mild chemical reaction conditions, leading to a significant reduction in contaminating ligand peptides while improving sensitivity and quantitative accuracy in analysis.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Yikang Zhou, Hang Han, Lijuan Zhang, He Huang, Ruiqiang Sun, Hang Zhou, Weichang Zhou
Summary: This study observed the accumulation of acetate in CHO cell culture, which may contribute to acidification, and proposed control strategies by manipulating temperature, glucose, and pH. The positive correlation between acetate generation rate and glucose consumption rate provides evidence of endogenous acetate generation. These findings have implications for improving process control and development in industrial CHO cell culture.
BIOTECHNOLOGY PROGRESS
(2023)
Article
Biochemistry & Molecular Biology
Simona Sedlackova, Martin Hubalek, Vladimir Vrkoslav, Miroslava Blechova, Petr Kozlik, Josef Cvacka
Summary: A typical bottom-up proteomic workflow often fails to fully describe certain domains or parts of proteins. This study introduces an alternative approach using acetylation and atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI) mass spectrometry. By acetylating peptides obtained through chymotrypsin or Glu-C digestion, APCI and APPI ionizations significantly improved the detection and sequence coverage of peptides compared to electrospray ionization (ESI). This method provides complementary data to classical bottom-up proteomics analysis.
Article
Biochemistry & Molecular Biology
Jeremy Loehr, Pata-Eting Kougnassoukou Tchara, Kevin Gonthier, Chahinez Noufi, Naomie Linteau, Etienne Audet-Walsh, Jean-Philippe Lambert
Summary: To study BRD-dependent interaction networks on chromatin, researchers established a series of acetate supplementation-dependent histone acetylation cell lines.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemical Research Methods
Jakob Woessmann, David Kotol, Andreas Hober, Mathias Uhlen, Fredrik Edfors
Summary: Using multiple proteases and recombinant protein standards, this study investigates the effect of protease bias on accuracy and precision in label-free mass spectrometry experiments. The researchers successfully established quantification methods for multiple proteins.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Food Science & Technology
Liuke Liang, Aowen Xie, Haojie Yang, Na Li, Ping'an Ma, Shan Wei, Shuaibing Zhang, Yangyong Lv, Yuansen Hu
Summary: Lysine acetylation (Kac) undergoes changes during artificial ageing of wheat seeds, affecting protein metabolism, response to intracellular stress, and protein storage and processing. Additionally, acetylation affects the activity of ROS-scavenging enzymes, leading to a gradual decrease in the defense ability of wheat seeds.
Article
Multidisciplinary Sciences
Ashraf Morsy, Amira S. Mahmoud, Aya Soliman, Hesham Ibrahim, Eman Fadl
Summary: Cellulose extracted from rice straw waste was transformed into nanocellulose acetate (NCA) to prepare cellulose acetate reverse osmosis (RO) membranes. The nanocellulose had a small particle size and spherical shape, while the developed membranes had a lower contact angle and enhanced hydrophobicity. The NCA: CA ratio of 40:60% in the membranes resulted in high salt rejection and water flux, as well as resistance to protein adsorption and microbial growth.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Liang Yin, Gang Feng, Chun Huang, Weijuan Cai
Summary: This study investigated lysine acetylation in T2DM patients using quantitative proteomics, identifying differentially expressed acetylation sites and performing bioinformatics analysis. The results provide potential markers for prediabetes prediction and contribute to the understanding of T2DM pathogenesis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shenglong Tian, Huihui Xie, Hui Zhang, Shiyu Fu
Summary: The article presents a simplified and green strategy for the synthesis of cellulose acetate, with greatly improved conversion rate and yield of the acetylated product achieved through hydrothermal treatment, resulting in significant enhancements in mechanical properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Plant Sciences
Rebecca A. Dewhirst, Joseph Lei, Cassandra A. Afseth, Cristina Castanha, Christina M. Wistrom, Jenny C. Mortimer, Kolby J. Jardine
Summary: The upregulation of acetate fermentation in plants as a drought survival strategy has been found to produce methyl acetate, which serves as a novel non-invasive tracer of temperature and drought survival responses within plants. This discovery could have significant implications for understanding how plants respond to drought at the transcription, cellular metabolism, and hormone signaling levels, as well as how it affects carbon cycling and water use at different scales.
Meeting Abstract
Biochemical Research Methods
Karin Mardo, Triinu Visnapuu, Anneli Aasamets, Katrin Viigand, Olesja Bondarenko, Heiki Vija, Kaarel Adamberg, Signe Adamberg, Tiina Alamae
Article
Biotechnology & Applied Microbiology
Kaspar Valgepea, Kim Q. Loi, James B. Behrendorff, Renato de S. P. Lemgruber, Manuel Plan, Mark P. Hodson, Michael Koepke, Lars K. Nielsen, Esteban Marcellin
METABOLIC ENGINEERING
(2017)
Article
Nutrition & Dietetics
Concetta Panebianco, Kaarel Adamberg, Signe Adamberg, Chiara Saracino, Madis Jaagura, Kaia Kolk, Anna Grazia Di Chio, Paolo Graziano, Raivo Vilu, Valerio Pazienza
Article
Biochemistry & Molecular Biology
Kaspar Valgepea, Renato de Souza Pinto Lemgruber, Kieran Meaghan, Robin William Palfreyman, Tanus Abdalla, Bjom Daniel Heijstra, James Bruce Behrendorff, Ryan Tappel, Michael Kopke, Sean Dennis Simpson, Lars Keld Nielsen, Esteban Marcellin
Article
Microbiology
Kaarel Adamberg, Signe Adamberg, Karin Emits, Anneli Larionova, Tiia Voor, Madis Jaagura, Triinu Visnapuu, Tiina Alamae
Article
Biotechnology & Applied Microbiology
Kaspar Valgepea, Renato de Souza Pinto Lemgruber, Tanus Abdalla, Steve Binos, Nobuaki Takemori, Ayako Takemori, Yuki Tanaka, Ryan Tappel, Michael Kopke, Sean Dennis Simpson, Lars Keld Nielsen, Esteban Marcellin
BIOTECHNOLOGY FOR BIOFUELS
(2018)
Article
Oncology
Concetta Panebianco, Kaarel Adamberg, Madis Jaagura, Massimiliano Copetti, Andrea Fontana, Signe Adamberg, Kaia Kolk, Raivo Vilu, Angelo Andriulli, Valerio Pazienza
CANCER CHEMOTHERAPY AND PHARMACOLOGY
(2018)
Article
Endocrinology & Metabolism
Renato de Souza Pinto Lemgruber, Kaspar Valgepea, Mark P. Hodson, Ryan Tappel, Sean D. Simpson, Michael Kopke, Lars K. Nielsen, Esteban Marcellin
Article
Microbiology
K. Adamberg, K. Kolk, M. Jaagura, R. Vilu, S. Adamberg
BENEFICIAL MICROBES
(2018)
Article
Food Science & Technology
Kaarel Adamberg, Madis Jaagura, Anu Aaspollu, Eha Nurk, Signe Adamberg
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
(2020)
Article
Biotechnology & Applied Microbiology
James K. Heffernan, Kaspar Valgepea, Renato de Souza Pinto Lemgruber, Isabella Casini, Manuel Plan, Ryan Tappel, Sean D. Simpson, Michael Kopke, Lars K. Nielsen, Esteban Marcellin
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Viia Koiv, Kaarel Adamberg, Signe Adamberg, Ingrid Sumeri, Sergo Kasvandik, Veljo Kisand, Ulo Maivali, Tanel Tenson
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Microbiology
Kaspar Valgepea, Gert Talbo, Nobuaki Takemori, Ayako Takemori, Christina Ludwig, Vishnuvardhan Mahamkali, Alexander P. Mueller, Ryan Tappel, Michael Kopke, Sean Dennis Simpson, Lars Keld Nielsen, Esteban Marcellin
Summary: Microbes that can recycle C-1 greenhouse gases into fuels and chemicals are crucial for the sustainability of future industries. In this study, the absolute proteomics technique was used to quantify protein concentrations in the acetogen Clostridium autoethanogenum, revealing the importance of proteome allocation for C-1 fixation.
Article
Microbiology
Lorena Azevedo de Lima, Kristina Reinmets, Lars Keld Nielsen, Esteban Marcellin, Audrey Harris, Michael Kopke, Kaspar Valgepea
Summary: RNA sequencing (RNA-seq) is a widely used technique for transcriptome analysis, but the choice of library preparation kits can significantly impact the results. Different commercial products show significant differences in rRNA removal, strand specificity, and transcript abundance distribution. Selecting appropriate sample processing workflows and products is crucial for RNA-seq studies.
MICROBIOLOGY SPECTRUM
(2022)
Article
Medical Laboratory Technology
Concetta Panebianco, Edgar Kelman, Kristel Vene, Domenica Gioffreda, Francesca Tavano, Raivo Vilu, Fulvia Terracciano, Illar Pata, Kaarel Adamberg, Angelo Andriulli, Valerio Pazienza
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2018)