Article
Green & Sustainable Science & Technology
Lucas Rodrigues Ramos, Giovanna Lovato, Jose Alberto Domingues Rodrigues, Edson Luiz Silva
Summary: The study found that operating the second-stage methanogenic reactor at mesophilic temperature in two-stage anaerobic digestion system can achieve the best energetic yield, as well as better stability and flexibility.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Biotechnology & Applied Microbiology
Nathaniel W. Fortney, Nathaniel J. Hanson, Paula R. F. Rosa, Timothy J. Donohue, Daniel R. Noguera
Summary: The economy of biorefineries is influenced by the production of valuable compounds from underutilized industrial residues, in addition to biofuel production from carbohydrates. Microbial communities grown on thin stillage (TS) can produce different fermentation products, and adjusting bioreactor operating conditions allows for the selection of different microbial communities with unique product profiles.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
H. D. D. Ziero, L. C. Ampese, L. S. Buller, V Oliani Trevisan, M. T. Gouvea, M. T. M. G. Rosa, M. D. Berni, T. Forster-Carneiro
Summary: The potential applications of by-products from corn ethanol mills are still in early stages of development in Brazil. This study analyzed a corn ethanol mill in Mato Grosso, Brazil, and found that anaerobic digestion of thin stillage could generate thermal and electric energy. Moreover, the research suggests that thin stillage anaerobic digestion could be a win-win alternative for corn ethanol mills in Brazil towards the circular economy approach.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Henrique Di Domenico Ziero, Larissa Castro Ampese, Luz Selene Buller, Josiel Martins Costa, Mauro Berni, Tania Forster-Carneiro
Summary: The generation of bioenergy from thin stillage in a corn ethanol mill provides a new perspective for the use of byproducts in a circular economy concept. Alternative technological routes for the corn ethanol by-products energy and nutrients recovery must be investigated to assess present and future decision-making toward economic and environmental indicators.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Energy & Fuels
Kurt A. Rosentrater, Lu Yang
Summary: Bio-based ethanol production has been a rapidly growing industry in the United States over the past decade. Therefore, it is necessary to explore wider uses of ethanol coproducts in ethanol plants. The study found that thin stillage and whole stillage have high moisture content and water activity, making them susceptible to rapid spoilage, while syrup has relatively low moisture content and water activity, and does not experience mold growth or separation.
FRONTIERS IN ENERGY RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Y. Zheng, D. B. Johnston, N. J. Engeseth, V. Singh, M. E. Tumbleson, K. D. Rausch
Summary: This study aims to develop a better understanding of the components that accelerate fouling of thin stillage evaporators and conducted three experiments to investigate the effects of carbohydrate materials and wet cake addition on evaporator fouling. The results showed that insoluble starch addition had larger effects than soluble sucrose addition on fouling resistances.
FOOD AND BIOPRODUCTS PROCESSING
(2021)
Article
Biochemistry & Molecular Biology
Cristiano Rodrigues E. Reis, Bo Hu
Summary: The study assessed a simplified scale-up approach of an ion-exchange separation system to extract phytate from thin stillage. The results showed that a simple and straightforward scale-up can be achieved by maintaining specific parameters.
Article
Biotechnology & Applied Microbiology
Andreas Zimmermann, Marvin Scherzinger, Martin Kaltschmitt
Summary: This study developed a three-stage process for the recovery of pentosan-type carbohydrates from bioethanol thin stillage, including solid-liquid separation, pentosan solubilisation, and purification. The feasibility of this process was demonstrated on a laboratory scale.
BIORESOURCES AND BIOPROCESSING
(2023)
Article
Environmental Sciences
Junqi Zhang, Deguang Wu, Yakun Zhao, Dingyuan Liu, Xuewu Guo, Cuiying Zhang, Xi Sun, Ju Guog, Dezhi Yuan, Dongguang Xiao, Feng Li, Hao Song
Summary: This study aimed to enhance power generation in microbial fuel cells by enabling S. oneidensis to co-utilize glucose and lactate from thin stillage. The researchers successfully constructed an engineered S. oneidensis strain and a 3D self-assembled biohybrid, resulting in the highest power density reported for genetically engineered S. oneidensis with thin stillage as a carbon source. This study provides a novel strategy for practical applications of microbial fuel cells in wastewater remediation and power recovery.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Wu Sun, Jingjing Xu, Zhongwei Yin, Huan Li, Juan Li, Li Zhu, Zhitao Li, Xiaobei Zhan
Summary: In this study, a novel polysaccharide (CRWP1) was purified from thin stillage, which showed prebiotic potential by promoting the growth of beneficial bacteria and the production of short-chain fatty acids. It also increased the production of organic acids and polyols.
PROCESS BIOCHEMISTRY
(2022)
Article
Green & Sustainable Science & Technology
Getachew D. Gebreeyessus, Andualem Mekonnen, Yonas Chebude, Esayas Alemayehu
Summary: The study characterized and quantified the products and byproducts of the sugar and ethanol industries, revealing gaps in research and policies while discussing sustainable alternatives. Despite the poor environmental performance, these industries discharge energy-rich waste streams directly into the environment, putting their sustainability at risk.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2021)
Article
Agricultural Engineering
Tana Tana, Tuyen H. T. Tran, Jerome Ramirez, Peter James Strong, Ian O'Hara, Jorge Beltramini, William O. S. Doherty, Lalehvash Moghaddam
Summary: The study focuses on converting second-generation cellulosic ethanol stillage into valuable products through hydrothermal liquefaction, achieving higher yields by recycling the aqueous phase, thus enhancing the efficiency and profitability of ethanol production. Oil stability tests showed significant drop in oil yield with aging, but no significant change in higher heating value.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Xiaohong Lu, Xiao Liu, Jinrong Hu, Ping Liu
Summary: The study found that using Aspergillus oryzae H18 treated thin stillage can increase alcohol production rate, and by adding proper concentration of nutrients, alcohol yield in yeast fermentation increased by 32.7%.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Article
Agronomy
Agata Bartkowiak, Joanna Lemanowicz, Magdalena Rydlewska, Olga DrabiNska, Karol Ewert
Summary: This study evaluated the fertilizing value of rye stillage in the cultivation of winter triticale and found that the use of stillage significantly increased the content of phosphorus, potassium, and magnesium in the soil, as well as the activity of alkaline phosphatase, acid phosphatase, and dehydrogenases. It also increased the organic carbon content in the soil and had no significant effect on pH. However, the response of enzymatic activity to stillage varied depending on the sampling season and soil depth, highlighting the importance of monitoring soil pH and rational application of mineral fertilization.
Article
Microbiology
Moussa Louati, Karima Hezbri, Maria del Carmen Montero-Calasanz, Manfred Rohde, Markus Goeker, Faten Ghodhbane-Gtari, Hans-Peter Klenk, Imen Nouioui, Maher Gtari
Summary: A new actinobacterium strain, Blastococcus tunisiensis sp. nov., was isolated from a limestone sample collected in Tunisia, which has distinct phenotypic and genotypic features.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Carl Simon Strittmatter, Anja Poehlein, Axel Himmelbach, Rolf Daniel, Alexander Steinbuchel
Summary: The number of genes encoding beta-oxidation enzymes in Cupriavidus necator H16 is high, but only two operons, A0459-A0464 and A1526-A1531, were expressed during growth with long-chain-length fatty acids (LCFAs). The deletion of these operons had a negative effect on growth with longer chain fatty acids. RNA-Seq analysis revealed the expression profiles of genes involved in the catabolism of medium-chain-length fatty acids (MCFAs) in C. necator H16.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Ines Friedrich, Alisa Kuritsyn, Robert Hertel, Rolf Daniel
Summary: The present study describes a novel species within the genus Luteibacter (EIF3(T)). The strain was analyzed genomically, morphologically and physiologically and identified as a new species of Luteibacter. In silico analysis indicated the presence of two putative prophages. The bacterial strain exhibits an elliptical morphotype with multiple flagella at one end and is resistant to certain antibiotics.
Article
Microbiology
Juan Guzman, Miyoung Won, Anja Poehlein, Atena Sadat Sombolestani, Daniela Mayorga-Ch, David Laureys, Jessika De Clippeleer, Peter Kaempfer, Rolf Daniel, Andreas Vilcinskas, Peter Vandamme, Soon- Wo Kwon
Summary: In this study, two strains of bacteria were isolated from wasps in Germany and Korea. Molecular and phenotypic analysis showed that these strains are closely related to known species but also have distinct characteristics. Therefore, it is proposed to name these strains Aristophania vespae.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Microbiology
Juan Guzman, Anja Poehlein, Rolf Daniel, Peter Kaempfer, Andreas Vilcinskas
Summary: Strain G314FT, isolated from Lucilia sericata in Germany, belongs to the genus Vagococcus. It shows high sequence identity with Vagococcus bubulae, Vagococcus martis, and Vagococcus teuberi, indicating that it represents a new species of the genus. The genome of G314FT is the smallest among the closely related species and has differences in amino acid biosynthesis and carbohydrate metabolism.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Delphine Clara Zemp, Nathaly Guerrero-Ramirez, Fabian Brambach, Kevin Darras, Ingo Grass, Anton Potapov, Alexander Roell, Isabelle Arimond, Johannes Ballauff, Hermann Behling, Dirk Berkelmann, Siria Biagioni, Damayanti Buchori, Dylan Craven, Rolf Daniel, Oliver Gailing, Florian Ellsaesser, Riko Fardiansah, Nina Hennings, Bambang Irawan, Watit Khokthong, Valentyna Krashevska, Alena Krause, Johanna Kueckes, Kevin Li, Hendrik Lorenz, Mark Maraun, Miryam Sarah Merk, Carina C. M. Moura, Yeni A. Mulyani, Gustavo B. Paterno, Herni Dwinta Pebrianti, Andrea Polle, Di Ajeng Prameswari, Lena Sachsenmaier, Stefan Scheu, Dominik Schneider, Fitta Setiajiati, Christina Ani Setyaningsih, Leti Sundawati, Teja Tscharntke, Meike Wollni, Dirk Hoelscher, Holger Kreft
Summary: There are still knowledge gaps in how to increase biodiversity and ecosystem functioning in cash crop-dominated tropical landscapes in the United Nations Decade on Ecosystem Restoration. This study presents findings from a 5-year ecosystem restoration experiment in an oil palm landscape enriched with 52 tree islands, showing higher indicators of biodiversity and ecosystem functioning in tree islands compared to conventionally managed oil palm. Enriching oil palm-dominated landscapes with tree islands is a promising ecological restoration strategy that does not decrease oil palm yield.
Article
Environmental Sciences
Jimyung Moon, Anja Schubert, Anja Poehlein, Rolf Daniel, Volker Mueller
Summary: To inactivate the Wood-Ljungdahl pathway in Acetobacterium woodii, two subunits of the methylene-tetrahydrofolate reductase were genetically deleted. The mutant was unable to grow on C1 compounds, lactate, ethanol, or butanediol, but could grow on fructose with lower rates and yields compared to the wild-type. Growth on fructose was restored by the addition of an external electron sink, such as glycine betaine + CO2 or caffeate. Transcriptome analyses revealed enzymes potentially involved in mixed acid fermentation (MAF) from fructose in A. woodii.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2023)
Article
Microbiology
Ines Friedrich, Hannes Neubauer, Alisa Kuritsyn, Bernhard Bodenberger, Faina Tskhay, Sara Hartmann, Anja Poehlein, Mechthild Boemeke, Michael Hoppert, Dominik Schneider, Robert Hertel, Rolf Daniel
Summary: By investigating host-associated metaviromes, we found that the classical isolation technique may miss the diversity of non-plaque forming phages. Through direct plaque-based isolation using new hosts, we discovered a higher phage diversity in Brevundimonas and Serratia. This study demonstrates the potential of combining metavirome analysis with traditional isolation techniques to reveal further phage diversity.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Environmental Sciences
Vannesa Montoya-Sanchez, Holger Kreft, Isabelle Arimond, Johannes Ballauff, Dirk Berkelmann, Fabian Brambach, Rolf Daniel, Ingo Grass, Jes Hines, Dirk Hoelscher, Bambang Irawan, Alena Krause, Andrea Polle, Anton Potapov, Lena Sachsenmaier, Stefan Scheu, Leti Sundawati, Teja Tscharntke, Delphine Clara Zemp, Nathaly Guerrero-Ramirez
Summary: Increasing landscape heterogeneity through establishing tree islands in oil-palm plantations can enhance multi-taxa diversity, particularly due to the increased presence of soil fauna and habitat diversity. Soil fauna, bacteria, and fungi have a stronger association with the overall community than aboveground taxa, emphasizing the importance of considering soil biota in strategies to enhance multi-taxa diversity.
COMMUNICATIONS EARTH & ENVIRONMENT
(2023)
Article
Microbiology
Raphael Trischler, A. Poehlein, R. Daniel, Volker Mueller
Summary: The human gut harbours a variety of microorganisms which play important roles in digestion, health and well-being. The genus Blautia, commonly found in the gut, has been shown to grow on glycerol and produce acetate and ethanol. This finding not only contributes to our understanding of microbial activity in the gut, but also has potential implications for bioindustrial production of bioethanol from waste glycerol.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Lydia Alberti, Patricia Koenig, Sabine Zeidler, Anja Poehlein, Rolf Daniel, Beate Averhoff, Volker Mueller
Summary: Acinetobacter baumannii, a nosocomial pathogen, exhibits significant metabolic diversity. Growth on L-arabinose was found, and transcriptome analyses identified two gene clusters responsible for the oxidation of pentonates to a-ketoglutarate. These clusters also encode an uptake system and a regulator for specific pentonate oxidation pathways. Despite a cross-talk between these pathways, growth on pentoses was observed in pathogenic Acinetobacter species, while growth on L-arabinose provided benefits for long-term survival and desiccation resistance in A. baumannii ATCC 19606.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Tim Boeer, Frank R. Bengelsdorf, Mechthild Boemeke, Rolf Daniel, Anja Poehlein
Summary: This study reports the complete genome sequences of acetogenic strains from the genus Terrisporobacter, along with functional annotation and pathway reconstruction analysis. The study also identifies species-specific nutrient requirements and constructs a model for acetogenesis in Terrisporobacter.
Article
Microbiology
Simone Anza, Dominik Schneider, Rolf Daniel, Michael Heistermann, Somboon Sangmaneedet, Julia Ostner, Oliver Schuelke
Summary: Maternal glucocorticoids (GCs) can have detrimental effects on offspring development, particularly during early gestation. This study found that high maternal GC levels during early gestation were associated with reduced bacterial richness in the offspring's gut microbiota. The effect of maternal GCs on the gut microbiota worsened with offspring age and was 10 times stronger than the effect of exposure during late prenatal or postnatal periods.
Article
Microbiology
Miriam Antonia Schueler, Rolf Daniel, Anja Poehlein
Summary: This study presents the complete genome of a non-toxigenic Clostridioides difficile strain isolated from horse feces. The genome consists of one chromosome and one plasmid, and encodes 3,798 putative genes. The strain represents a sub-cluster in the cryptic clade C-III.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Ecology
Benjamin Zeldes, Anja Poehlein, Surbhi Jain, Christoph Baum, Rolf Daniel, Volker Mueller, Mirko Basen
Summary: DNA uptake is a common mechanism in microorganisms for rapid adaptation to new environments. This study found that a thermophilic bacterium, Thermoanaerobacter kivui, adapted to high concentrations of carbon monoxide through a horizontal gene transfer event with another thermophile, Thermoanaerobacter sp. strain X514, within the laboratory. This transfer also led to the deletion of a native operon for thiamine biosynthesis, explaining why the adapted strain required added vitamins for growth.
ISME COMMUNICATIONS
(2023)
Article
Microbiology
Jacqueline Hollensteiner, Dominik Schneider, Anja Poehlein, Axel Himmelbach, Rolf Daniel
Summary: This study reports 10 particle-associated metagenome-assembled genomes (MAGs) from the mesopelagic zone of Pacific Ocean seawaters. Functional annotation suggests that these genomes are involved in central particulate organic carbon conversion, nitrogen cycling, and phosphorus cycling.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)