Article
Microbiology
Marco Antonio Lopez Marin, Michal Strejcek, Petra Junkova, Jachym Suman, Jiri Santrucek, Ondrej Uhlik
Summary: The study shows that using a Micrococcus luteus culture supernatant containing resuscitation-promoting factor (SRpf) can increase the number and diversity of cultured bacterial taxa from a nutrient-rich compost soil. After SRpf treatment, potentially novel bacterial species were isolated, demonstrating the effectiveness of SRpf in enhancing the growth of soil bacterial species.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Ashutosh Kabiraj, Urmi Halder, Annapurna Chitikineni, Rajeev K. Varshney, Rajib Bandopadhyay
Summary: Contamination of arsenic in drinking water and foods is a threat to human beings. Researchers have been studying the bioremediation of arsenic using potent bacteria to reduce its availability. In this study, a strain of Micrococcus luteus was isolated from arsenic-contaminated water in West Bengal, India. This strain showed tolerance to high concentrations of arsenic and other metals, as well as the ability to adsorb arsenic and promote plant growth. These findings suggest its potential application in arsenic bioremediation.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Engineering, Environmental
Xinru Zhou, Shusheng Zhang, Rui Wang, Zijing An, Faqian Sun, Chaofeng Shen, Hongjun Lin, Xiaomei Su
Summary: The study found that Rpf of Micrococcus luteus can resuscitate and stimulate the growth of PCB-degrading bacterial populations, thereby improving the bioremediation efficiency of PCBs. Soil microcosms amended with Rpf and LS1 degraded PCBs 1.1-3.2 times faster than control microcosms. The degradation process mainly occurred through the HOPDA-benzoate-catechol/protocatechuate pathways. These results suggest that Rpf and resuscitated strains serve as effective additives and bio-inoculants for enhanced bioremediation, providing new approaches for large-scale applications of in situ bioremediation.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Xiaomei Su, Si Li, Mengqi Xie, Linqin Tao, Yan Zhou, Yeyuan Xiao, Hongjun Lin, Jianrong Chen, Faqian Sun
Summary: Using Rpf from Micrococcus luteus can enhance the biodegradation of PCBs in contaminated environments by resuscitating and stimulating functional bacterial populations. It was found that Rpf significantly altered the composition and abundance of bacterial populations in the phylum Proteobacteria, with Sphingomonas and Pseudomonas identified as key PCB-degraders. These results suggest that Rpf has the potential to improve the efficiency of bioremediation processes in PCB-contaminated environments.
Article
Chemistry, Multidisciplinary
Mengqi Xie, Yilin Li, Luning Xu, Shusheng Zhang, Hongyu Ye, Faqian Sun, Rongwu Mei, Xiaomei Su
Summary: This study optimized Rpf production using the RSM method and found that the highest Rpf protein yield could be obtained under certain conditions. Additionally, the Phyre2 web portal characterized the structure of the Rpf domain to have shared homology with lysozymes, with the highest lysozyme activity achievable under specific conditions.
Article
Biotechnology & Applied Microbiology
Xiaomei Su, Mengqi Xie, Zhen Han, Yeyuan Xiao, Rui Wang, Chaofeng Shen, Muhammad Zaffar Hashmi, Faqian Sun
Summary: This study discovered the enhancement of Rpf on the anaerobic microbial dechlorination of PCE/PCBs and uncovered the underlying Rpf-responsive populations. It reveals the possibility of rapidly enriching active dechlorinating cultures through Rpf amendment, providing a new approach for massively enriching PCB dechlorinators in short periods of time.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Attila Bodor, Naila Bounedjoum, Gabor Feigl, Agnes Duzs, Krisztian Laczi, Arpad Szilagyi, Gabor Rakhely, Katalin Perei
Summary: The study showed that using extracellular organic matter (EOM) from Micrococcus luteus to enhance bioremediation of ULO pollution was effective, with a significant increase in microbial activity and degradation efficiency. High-throughput 16S rRNA analysis revealed that the enhancement was attributed to the reactivation of hydrocarbonoclastic bacterial genera and the long-term positive effect of EOM on the introduced rhodococci. Reducing ULO concentration did not necessarily correlate with decreased soil toxicity.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
J. Fu, J. Chen, Y. Wang, T. Meng, L. Yue, D. Luo, X. Wang
Summary: The study demonstrated that the recombinant protein Rpf2 has muramidase and protease activities, significantly enhancing the diversity and efficiency of petroleum-degrading bacteria in soil.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Qingmiao Yu, Xianda Hu, Fuzheng Zhao, Chenyu Zhu, Linchang Guan, Hongqiang Ren, Jinju Geng
Summary: This study analyzed the effect of dissolved organic matter (DOM) composition on the removal efficiency of NGT in wastewater treatment plants (WWTPs). The results showed that DOM composition was a significant factor in NGT removal, and its effect was mediated by regulating core microbial communities.
Article
Environmental Sciences
Yangyang Jia, Xinyi Li, Fengjun Xu, Zefan Liu, Yulong Fu, Xin Xu, Jiawen Yang, Shuai Zhang, Chaofeng Shen
Summary: This study developed a new strategy to isolate biphenyl-degrading bacteria from soil by integrating single-cell-level isolation with micro-fluidics technology and culture with resuscitation-promoting factor (Rpf). The results provide important insights into enriching target functional microorganisms, enhancing the potential for bioremediation of contaminated soils, and expanding our understanding of the response mechanisms to exogenous Rpf.
ENVIRONMENTAL POLLUTION
(2022)
Article
Engineering, Environmental
Xiuli Chen, Yanchu Ke, Ying Zhu, Mingbang Xu, Chao Chen, Shuguang Xie
Summary: Two TC-degrading microbial consortia were enriched from activated sludge and soil, showing high degradation capabilities under a wide pH range and at moderate/high temperatures. Metagenomic sequencing revealed genes related to TC biodegradation.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Xinyu Song, Yue Zhao, Linyuan Zhang, Xinyu Xie, Junqiu Wu, Zimin Wei, Hongyu Yang, Shubo Zhang, Caihong Song, Liming Jia
Summary: This study found that bacteria play a key role in the degradation of DOM in the summer water column, while photochemical degradation is the primary pathway in the autumn and winter water columns. Additionally, photosynthetic bacteria produce organic matter through photosynthesis to replenish the water column during DOM degradation.
Article
Microbiology
Daniela Figueroa, Eric Capo, Markus V. Lindh, Owen F. Rowe, Joanna Paczkowska, Jarone Pinhassi, Agneta Andersson
Summary: Climate change is projected to increase the inflow of terrestrial organic matter to coastal areas in northerly regions, affecting estuarine bacterial community composition and ecological functions. Specialist bacteria were favored by freshly discharged organic matter in spring, while a mix of autochthonous and terrestrial organic matter promoted generalist development in summer.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yue Yang, Ruyan Xu, Mengmeng Yang, Qiang Xu, Chenlin Miao, Jianhua Guo, Wenjun Mou, Hang Du, Gang Wei, Liwei Hu, Zongyu Hu
Summary: This study analyzed the changes in bacterial community and structural composition of tobacco leaves from flue-curing to redrying, and found that flue-curing had a greater effect on the microbial community than redrying. The relative abundance of pathway related to metabolism increased after flue-curing, while the relative abundance of pathway related to environmental information processing increased after redrying.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Yixin Zhou, Zhichao Shi, Qiliang Pang, Xiufeng Liang, Hongtao Li, Xin Sui, Chongwei Li, Fuqiang Song
Summary: Mycorrhiza helper bacteria (MHB) play a crucial role in fruiting-body formation by enhancing bacterial diversity and chemical properties in the soil. The bacterial community structure and diversity were higher in mycorrhizal rhizosphere soil compared to non-mycorrhizal rhizosphere soil. Specific bacteria such as Burkholderia, Bradyrhizobium, Pseudomonas, and Rhizobium were more abundant in mycorrhizal rhizosphere soil. The soil organic matter, total nitrogen, total phosphorus, total potassium, ammonium nitrogen, available phosphorus, available potassium, and enzyme activity were also significantly higher in mycorrhizal rhizosphere soil. Redundancy analysis indicated that dominant bacteria were closely associated with soil enzyme activity and physicochemical properties.
Article
Environmental Sciences
Jiafang Cai, Aodong Pan, Yilin Li, Yeyuan Xiao, Yan Zhou, Chongjun Chen, Faqian Sun, Xiaomei Su
Summary: The study explored a novel method of using resuscitation-promoting factors (Rpfs) to enhance the degradation efficiency of the dye RB19 in anaerobic processes. Results showed that Rpfs efficiently promoted RB19 decolorization, increasing decolorization efficiency by over 20%. Additionally, Rpfs appeared to stimulate and enrich dye-degrading species of the family Peptostreptococcaceae, leading to higher RB19 decolorization efficiency.
Article
Environmental Sciences
Xiaomei Su, Si Li, Mengqi Xie, Linqin Tao, Yan Zhou, Yeyuan Xiao, Hongjun Lin, Jianrong Chen, Faqian Sun
Summary: Using Rpf from Micrococcus luteus can enhance the biodegradation of PCBs in contaminated environments by resuscitating and stimulating functional bacterial populations. It was found that Rpf significantly altered the composition and abundance of bacterial populations in the phylum Proteobacteria, with Sphingomonas and Pseudomonas identified as key PCB-degraders. These results suggest that Rpf has the potential to improve the efficiency of bioremediation processes in PCB-contaminated environments.
Article
Biotechnology & Applied Microbiology
Mengqi Xie, Luning Xu, Rong Zhang, Yan Zhou, Yeyuan Xiao, Xiaomei Su, Chaofeng Shen, Faqian Sun, Muhammad Zaffar Hashmi, Hongjun Lin, Jianrong Chen
Summary: The study revealed that a yeast strain capable of degrading phenol entered the viable but nonculturable (VBNC) state under high concentrations of contaminants, and the resuscitation of VBNC cells restored their degradation performance. Compared to normal cells, significant morphological, surface, and physiological changes were observed in the VBNC cells.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Environmental Sciences
Muhammad Zaffar Hashmi, Muhammad Kalim, Umar Farooq, Xiaomei Su, Paromita Chakraborty, Shams Ur Rehman
Summary: This review paper discusses the recent development in photocatalytic and chemical techniques for the remediation of PCBs in contaminated soils. Advanced oxidation is found to be an efficient remediation technique with high removal efficiency of PCBs. Persulfate oxidation is identified as a suitable technique that can work under normal environmental conditions. Factors such as pH, temperature, and the use of surfactants and organic solvents affect the effectiveness of these techniques.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Siying Sun, Jiabao Ren, Jiale Liu, Liang Rong, Hangli Wang, Yeyuan Xiao, Faqian Sun, Rongwei Mei, Chongjun Chen, Xiaomei Su
Summary: The study found that pyrite-activated persulfate oxidation efficiently removed organic matter from the effluent of a biological landfill leachate treatment system, and subsequent denitrification was also successful. With the optimal parameters, a COD removal efficiency of about 45% and a nitrate removal rate of around 108 mg L-1 d(-1) were achieved.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Environmental Sciences
Yilin Li, Wenjia Zhang, Yun Dai, Xiaomei Su, Yeyuan Xiao, Dan Wu, Faqian Sun, Rongwu Mei, Jianrong Chen, Hongjun Lin
Summary: This study investigated the start-up, influencing factors, and stable operation of partial denitrification for treating biological effluent from landfill leachate. The research found a suitable ratio of nitrate/ammonium for downstream Anammox process, and highlighted the importance of carbon sources, COD/NO3--N ratio, and salinity in partial denitrification.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Qihua Lin, Xinru Zhou, Shusheng Zhang, Junliang Gao, Mengqi Xie, Linqin Tao, Faqian Sun, Chaofeng Shen, Muhammad Zaffar Hashmi, Xiaomei Su
Summary: Functional microorganisms in a viable but non-culturable state limit the study of PCB-degrading strains. This study found that the resuscitated strain SPC0 from genus Streptococcus has the potential to degrade PCBs through the biphenyl pathway, highlighting the powerful capability of resuscitated strains for bioremediation of PCB-contaminated sites.
ENVIRONMENTAL RESEARCH
(2022)
Review
Biotechnology & Applied Microbiology
Mayada Jamal, Annoor Awadasseid, Xiaomei Su
Summary: Anthraquinone dyes, widely used in textile manufacturing, pose a significant pollution threat due to their complex and stable structures. Microbiological treatment of wastewater has emerged as an economically viable solution, with promising advances in the biodegradation of anthraquinone dyes.
BIOTECHNOLOGY LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Xiaomei Su, Mengqi Xie, Zhen Han, Yeyuan Xiao, Rui Wang, Chaofeng Shen, Muhammad Zaffar Hashmi, Faqian Sun
Summary: This study discovered the enhancement of Rpf on the anaerobic microbial dechlorination of PCE/PCBs and uncovered the underlying Rpf-responsive populations. It reveals the possibility of rapidly enriching active dechlorinating cultures through Rpf amendment, providing a new approach for massively enriching PCB dechlorinators in short periods of time.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Environmental Sciences
Zhen Han, Qihua Lin, Shusheng Zhang, Xinru Zhou, Si Li, Faqian Sun, Chaofeng Shen, Xiaomei Su
Summary: The study found that the resuscitated strain LS1 has the potential to degrade polychlorinated biphenyls (PCBs), with better performance in degrading PCB 18 and 52. LS1 exhibited lower efficiency in degrading PCB 77. Additionally, LS1 can survive and remain culturable in PCB-contaminated soil for up to 70 days.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Xinru Zhou, Shusheng Zhang, Rui Wang, Zijing An, Faqian Sun, Chaofeng Shen, Hongjun Lin, Xiaomei Su
Summary: The study found that Rpf of Micrococcus luteus can resuscitate and stimulate the growth of PCB-degrading bacterial populations, thereby improving the bioremediation efficiency of PCBs. Soil microcosms amended with Rpf and LS1 degraded PCBs 1.1-3.2 times faster than control microcosms. The degradation process mainly occurred through the HOPDA-benzoate-catechol/protocatechuate pathways. These results suggest that Rpf and resuscitated strains serve as effective additives and bio-inoculants for enhanced bioremediation, providing new approaches for large-scale applications of in situ bioremediation.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Qihua Lin, Jiayan Ding, Yingying Yang, Faqian Sun, Chaofeng Shen, Hongjun Lin, Xiaomei Su
Summary: The feasibility of utilizing an immobilization approach to enhance the PCBs degradation performance of a resuscitated strain Streptococcus sp. SPC0 was evaluated in this study. The results showed that the mixed matrix of polyvinyl alcohol (PVA) and sodium alginate (SA) provided a porous microstructure for SPC0 colonization and proliferation, leading to enhanced removal of PCBs through simultaneous adsorption and biodegradation. Immobilized SPC0 demonstrated 1.0-7.1 times higher PCB removal than free cells, with higher concentrations achieving more effective removal. Moreover, the immobilized SPC0 showed excellent stability and reusability after four cycles of reuse.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Green & Sustainable Science & Technology
Rong Zhang, Min Zhang, Huaqian Mou, Zijing An, Hailu Fu, Xiaomei Su, Chongjun Chen, Jianrong Chen, Hongjun Lin, Faqian Sun
Summary: Kitchen waste biochar showed better performance in thermophilic co-digestion, reducing the digestion time and increasing methane production. It also improved the microbial community structure and enhanced the acetoclastic methanogenesis in thermophilic co-digestion.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Environmental Sciences
Rui Wang, Zijing An, Lu Fan, Yan Zhou, Xiaomei Su, Junjie Zhu, Qian Zhang, Chongjun Chen, Hongjun Lin, Faqian Sun
Summary: Quorum quenching (QQ) is a novel technique for controlling biofouling in membrane bioreactors (MBRs) by disrupting quorum sensing (QS) and inhibiting biofilm formation. This study evaluated the effectiveness of the QQ strain Brucella sp. ZJ1, encapsulated in alginate beads, in mitigating biofouling. The results showed that MBR with QQ beads extended operation time by 2-3 times without affecting pollutant degradation, and these beads maintained approximately 50% QQ activity even after 50 days of operation.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Environmental Sciences
Junliang Gao, Rui Wang, Yilin Li, Huimin Huang, Xiaomei Su, Zijing An, Wenjun Yin, Lizhen Yang, Liang Rong, Faqian Sun
Summary: The effect of two aeration modes in the aerobic phase on nitrogen removal and N2O emission in treating landfill leachate was compared. Increasing dissolved oxygen showed positive effects on nitrogen removal and minimized N2O emission. However, high dissolved oxygen concentration reduced nitrogen removal and led to high N2O emission.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)