Article
Biochemistry & Molecular Biology
Zhi-hao Su, Jun-li Lv, Qi Ou, Zi-qin Zhao, Kai-yi Zheng, Xiao-ying Zhang, Wen-qing Lai, Xue-yang Wang, Ming-jie Deng, Mu-wang Li
Summary: The white epidermis of silkworms is caused by uric acid crystals accumulation. Abnormal metabolism of uric acid reduces its production, resulting in a transparent or translucent phenotype. This study examined the metabolite changes following Bombyx mori nucleopolyhedrovirus (BmNPV) infection in two strains of silkworms (p50 and op50) and found that the uric acid pathway plays a critical role in resistance. Feeding with inosine, a metabolite in this pathway, significantly enhanced larval resistance and modulated other metabolic pathways. The results provide insights into the resistance mechanism of silkworms to BmNPV and offer new strategies for pest control.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Fisheries
Xiu Shi, Yaxin Zhang, Tianchen Zhu, Nan Li, Sufei Sun, Min Zhu, Jun Pan, Zeen Shen, Xiaolong Hu, Xing Zhang, Chengliang Gong
Summary: The study utilized 16S rDNA sequencing and metabolomics to show significant alterations in gut microbiota and metabolites of silkworm midgut after BmNPV infection. Analysis revealed enrichment of specific pathways related to viral infection and metabolism, highlighting the relationship between digestive tract gut microbiota, metabolites, and host-virus interaction. These findings enhance understanding of viral infection links to midgut microbiota and metabolic activity in the silkworm model.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Feifei Zhu, Dandan Song, Han Chen, Qi Tang, Shuhao Huo, Xiaoyong Liu, Keping Chen
Summary: Lipids have been suggested to play a crucial role in virus entry and infection. A comprehensive lipidomic analysis using UPLC-MS was conducted on susceptible and resistant silkworm strains, revealing key lipids associated with virus infection and immune response. The study highlights the relationship between lipid biosynthesis, viral resistance, and immune response in the host.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Qian Wang, Miao Cui, Cong Zhang, Anqi Xia, Qiang Wang, Xiaoyong Liu, Keping Chen, Hengchuan Xia
Summary: The cloning of the SOCS7 gene in the silkworm and its role in the immune response against infection were investigated in this study.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Entomology
Kakeru Yokoi, Takuya Tsubota, Akiya Jouraku, Hideki Sezutsu, Hidemasa Bono
Summary: In this study, reference transcriptome data for the silkworm was established and detailed examination of gene expression in silkworm tissues was performed. The results contribute to understanding silkworm biology and promoting industrial applications.
Article
Entomology
Xiong Wang, Guangyu Ma, Feifei Ren, Mian Muhammad Awais, Jingchen Sun
Summary: Research has shown that Bombyx mori fatty acid binding protein 1 (BmFABP1) is expressed in silkmoth cells and in the tissues of silkmoth larvae, where it inhibits the replication of Bombyx mori nucleopolyhedrovirus (BmNPV). This study provides new insights into the potential antiviral role of BmFABP1 and its importance in breeding BmNPV-resistant transgenic silkmoths.
Article
Microbiology
Yanting Liang, Weifan Xu, Yanyan Zhou, Yun Gao, Huan Tian, Xiaofeng Wu, Yusong Xu, Huabing Wang
Summary: The protein BmSUH plays a crucial role in the oral infection of BmNPV by mediating the entry of ODVs into the midgut. This study provides insights into preventing BmNPV infection through disrupting essential protein-protein interactions.
Article
Virology
Mudasir Gani, Sergei Senger, Satish Lokanath, Pawan Saini, Kamlesh Bali, Rakesh Gupta, Vankadara Sivaprasad, Johannes A. Jehle, Joerg T. Wennmann
Summary: The study revealed the distribution of Indian BmNPV and investigated their genetic composition through methods like gene sequencing and phylogenetic analysis. It found genetic variations among different isolates and identified some new closely related viral strains.
Article
Biotechnology & Applied Microbiology
Andrea L. Livieri, Facundo Colaccini, Martin A. Hernandez, Gabriela Gago, Hector M. Alvarez, Hugo Gramajo, Eduardo Rodriguez
Summary: In actinomycetes, acyl-CoA carboxylases (ACCs) are biotin-dependent enzymes with diverse structures and functions. Rhodococcus jostii RHA1 was found to have multiple putative ACC domains and subunits. Mutant strains with specific genes disrupted showed the importance of these genes in lipid biosynthesis and growth.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Entomology
Kohei Kakino, Hiroaki Mon, Takeru Ebihara, Masato Hino, Akitsu Masuda, Jae Man Lee, Takahiro Kusakabe
Summary: This study characterizes the gene expression patterns and function in the testis of Bombyx mori, revealing peculiar features in spermatogenesis. The transcriptome analysis identifies widespread gene expression and testis-specific genes in B. mori, and compares it with Drosophila melanogaster for a better understanding. The study fills a knowledge gap and provides a basis for future research in spermatogenesis.
Article
Biochemistry & Molecular Biology
Ying-Xue Liu, Jia-Yue Yang, Jun-Long Sun, An-Cheng Wang, Xing-Ya Wang, Lin-Bao Zhu, Hui-Hua Cao, Zhi-Hao Huang, Shi-Huo Liu, Jia-Ping Xu
Summary: In this study, it is found that BmFerHCH regulates BmNPV infection through the JNK signaling pathway, which is activated by ROS. Moreover, the JNK pathway is involved in BmNPV-induced apoptosis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Virology
Guanping Chen, Yuedong Li, Xiangshuo Kong, Shudi Zhao, Jiale Li, Xiaofeng Wu
Summary: This study found that the expression of Bombyx mori HEXIM1 (BmHEXIM1) was significantly upregulated after BmNPV infection. By suppressing BmRelish-driven immune responses, BmHEXIM1 assisted in the immune escape of BmNPV. This research provides new insights into the mechanisms of immune escape of BmNPV and theoretical guidance for the breeding of BmNPV-resistant silkworm varieties.
Article
Agriculture, Multidisciplinary
Buli Su, Peixuan Lai, Fan Yang, Anzhang Li, Ming-Rong Deng, Honghui Zhu
Summary: This study developed a novel method to increase the supply of acetyl-CoA in Saccharomyces cerevisiae and improve the production of downstream products. By optimizing metabolic pathways and conducting genetic engineering, the production of mevalonic acid was successfully increased, and further improvement was achieved by dynamically downregulating gene expression. The final strain produced a high yield of lycopene.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Linrong Wan, Wenfu Xiao, Ziyan Huang, Anlian Zhou, Yaming Jiang, Bangxing Zou, Binbin Liu, Cao Deng, Youhong Zhang
Summary: This article systematically identified and analyzed small open reading frames (smORFs) in the silkworm, finding 738 highly reliable smORFs and 34,401 possible smORFs. It also identified differentially expressed and tissue-specific-expressed smORFs, which may be closely related to tissue characteristics and functions.
Article
Entomology
Jun-Qing Ge, Zhu-Hong Wang, Xi Chen, Hua Chen, Jian Huang
Summary: BmNPV p26 is conserved among all completely sequenced Lepidoptera baculoviruses and may play an important role in the virus infection cycle, with functions in both the cytoplasm and nucleus. RNAi-based knockdown of p26 decreased budded virus production in BmNPV-infected cells and may be associated with BmNPV replication during the late infection stage.
Article
Biotechnology & Applied Microbiology
Tatsuya Kato, Ami Yokomori, Riho Suzuki, Junya Azegami, Hesham A. El Enshasy, Enoch Y. Park
Summary: The proteasome inhibitor MG-132 was found to suppress riboflavin production and SDH activity in A. gossypii, indicating a negative influence on flavoprotein homeostasis. These results elucidate the connection between riboflavin production and flavoproteins in the organism.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Jirayu Boonyakida, Doddy Irawan Setyo Utomo, Fahmida Nasrin Soma, Enoch Y. Park
Summary: This study successfully expressed recombinant VP1 of norovirus in silkworms, resulting in the formation of highly purified NoV-LPs. The purified VLPs were shown to be structurally correct, biologically functional, and exhibited potential applications in a vaccine platform. Furthermore, the silkworm-based expression system is known for its robustness, scalability, and cost-effectiveness compared to insect cell systems.
PROTEIN EXPRESSION AND PURIFICATION
(2022)
Article
Chemistry, Analytical
Gahyeon Kim, Jinmyeong Kim, Soo Min Kim, Tatsuya Kato, Jinho Yoon, Seungwoo Noh, Enoch Y. Park, Chulhwan Park, Taek Lee, Jeong-Woo Choi
Summary: A MERS-nanovesicle biosensor with multifunctional DNA aptamer was developed for detecting MERS-NVs in different solutions, offering a new approach for MERS-CoV detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biotechnology & Applied Microbiology
Doddy Irawan Setyo Utomo, Sabar Pambudi, Enoch Y. Park
Summary: Dengue, a mosquito-borne disease, poses a significant global public health challenge. Researchers have successfully produced dengue virus-like particles using silkworms, which share similar epitopes with the natural dengue virus and elicit a stronger immune response.
Article
Chemistry, Multidisciplinary
Ojodomo J. Achadu, Njemuwa Nwaji, Dongkyu Lee, Jaebeom Lee, Eser M. Akinoglu, Michael Giersig, Enoch Y. Park
Summary: The novel hierarchically porous 3D magnetic molybdenum trioxide-polydopamine-gold functionalized nanosphere (3D mag-MoO3-PDA@Au NS) shows great potential for pathogen detection, overcoming challenges in Raman signal stability and reproducibility, and achieving ultrasensitive detection of SARS-CoV-2 spike protein.
NANOSCALE ADVANCES
(2022)
Article
Biochemistry & Molecular Biology
Jian Xu, Takafumi Nakanishi, Tatsuya Kato, Enoch Y. Park
Summary: The Baculovirus expression vector system (BEVS) is an effective protein expression system and has been used to engineer important enzymes and vaccines. The use of fusion tags for protein purification can negatively affect the biological activity of the target protein. This study validated the cleavage activity of the human rhinoviruses (HRV) 3C protease in both Escherichia coli and silkworm-BEVS systems. The findings demonstrate the successful establishment of a tag-free protein engineering platform using a coinfection-based recognition-and-reaction BEVS system.
BIOSCIENCE REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Tatsuya Kato, Tatsuki Kakuta, Ami Yonezuka, Tomofumi Sekiguchi, Yuki Machida, Jian Xu, Tohru Suzuki, Enoch Y. Park
Summary: In this study, silkworm larvae were used to express VP6 and VP7 proteins of porcine rotavirus A. The results showed that VP6 had a higher expression level and formed larger nanoparticles compared to VP7. This indicates the potential use of VP6 in vaccine development against porcine rotavirus, with silkworm larvae as a promising production host.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jirayu Boonyakida, Indra Memdi Khoris, Fahmida Nasrin, Enoch Y. Park
Summary: This study aimed to develop a modular display platform for protein decoration on norovirus-like particles (NoV-LPs) by combining the NoVLP scaffold with the SpyTag/SpyCatcher bioconjugation system. Results showed that adding an extension linker increased the conjugation efficiency and level of protein decoration on the NoV-LPs. This study provides a strategy for enhancing the conjugation efficiency of NoV-LP and demonstrates the platform's utility for developing vaccines or functional nanoparticles.
Article
Chemistry, Analytical
Akhilesh Babu Ganganboina, Indra Memdi Khoris, Akinori Konno, Tian-Cheng Li, Akihiro Okamoto, Enoch Y. Park
Summary: This article describes the design and construction of a visible light-driven photoelectrochemical (PEC) device based on a CdSe-Co3O4@TiO2 nanoflower. The device demonstrated ultrasensitive detection of viruses including hepatitis E virus (HEV), HEV-like particles (HEV-LPs), and SARS-CoV-2 spike protein in complex lysate solution. The enhanced photocurrent response of the PEC device can be attributed to the CdSe quantum dot sensitization effect and strong visible light absorption, improving system stability and energy conversion efficiency. The device achieved considerable sensitivity, good specificity, and acceptable stability, indicating its potential for affordable and portable biosensing capabilities.
Article
Biochemical Research Methods
Fahmida Nasrin, Indra Memdi Khoris, Ankan Dutta Chowdhury, Sjakurrizal El Muttaqein, Enoch Y. Park
Summary: To prevent viral diseases, the development of a disposable, rapid, and convenient biosensor with high sensitivity and reliability is highly desired. In this study, a practical impedimetric biosensor has been created on a disposable electrode for the detection of two mosquito-borne viruses. The biosensor fabrication involves the formation of a conductive film, physical binding of gold nanoparticles-polyaniline, and biofunctionalization. The disposable electrode has been designed to detect Chikungunya virus and Zika virus separately with a wide linear range and low detection limits.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Tatsuya Kato, Mai Kano, Ami Yokomori, Junya Azegami, Hesham A. El Enshasy, Enoch Y. Park
Summary: The mitochondrial membrane potential of the riboflavin-overproducing Ashbya gossypii mutant was decreased compared to the wild type strain, resulting in increased reactive oxygen species. It was discovered that flavoproteins and branched-chain amino acids play crucial roles in riboflavin production.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Jian Xu, Tomofumi Sekiguchi, Jirayu Boonyakida, Tatsuya Kato, Enoch Y. Park
Summary: Significant progress has been made in decorating virus-like particles (VLPs) with functional molecules, such as antigens or nucleic acids. This study focuses on the expression and engineering of the capsid protein VP2 of canine parvovirus for VLP display in the silkworm-expression system. The results show that the VP2 variant with a specific insertion of SpyTag at the L2 region significantly enhances VLP display, while the variant with SpyTag at the Lx region fails to form VLPs. Further verification is needed to assess its capacity for displaying desired antigens and inducing immune responses.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Marina Ikegaya, Enoch Y. Park, Takatsugu Miyazaki
Summary: Glycoside hydrolase family 31 (GH31) contains a-glycoside hydrolases with different substrate specificities involved in various physiological functions. This study characterized two a-galactosidases, BsGH31_19 and FpGH31_19, and found that they showed high substrate specificity against a-(1?4)-linkages in a-(1?4)-galactobiose and globotriose, unlike other reported GH31 a-galactosidases PsGal31A and MYORG. Structural analyses revealed differences in amino acid residues and dimer interfaces between BsGH31_19 and FpGH31_19 compared to PsGal31A and MYORG, providing insights into the molecular diversity and evolutionary relationships of GH31 a-galactosidases and other a-galactosidase families.
Review
Chemistry, Analytical
Sjaikhurrizal El Muttaqien, Indra Memdi Khoris, Sabar Pambudi, Enoch Y. Y. Park
Summary: In the past few decades, nanomaterials have been explored in the sensing research field. However, less attention has been given to their application in biosensors for pathogenic virus detection. This review focuses on 3D nanospheres, a type of nanostructured material, and their extensive use in various sensing techniques. Signal amplification strategies using different types of nanospheres are discussed, along with challenges related to toxicity and commercialization in the development of nanosphere-based biosensors.