Article
Microbiology
Cun-Cui Kong, Xin Wei, Guang-Lei Liu, Zhen-Ming Chi, Zhe Chi
Summary: In this study, the strain Aureobasidium melanogenum HN6.2 was genetically engineered to enhance siderophore production, ornithine synthesis, and putrescine production. The overexpression of specific genes successfully increased the yields of ornithine and putrescine, making this strain a promising candidate for industrial applications.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Reproductive Biology
Christopher L. J. Lavergne, Yong Tao, Yanping Ren, Nahantara Lafleur, X. Johne Liu
Summary: In mammalian species, the ovaries produce ODC and putrescine during ovulation. Aged mice have reduced levels of periovulatory ODC and putrescine. Supplementation of putrescine improves fertility in aged mice. However, increasing putrescine levels may lead to unwanted side effects. Attempts to increase ovarian putrescine levels through L-ornithine supplementation did not yield desired results.
BIOLOGY OF REPRODUCTION
(2022)
Article
Food Science & Technology
Zhe Xu, Jiale Chang, Jiamin Zhou, Yixin Shi, Hui Chen, Lingyu Han, Maolin Tu, Tingting Li
Summary: This study found that the ornithine decarboxylase gene (ODC) is involved in putrescine biosynthesis and successfully expressed it in E. coli. Functional analysis confirmed that the ODC protein can catalyze the decarboxylation of ornithine to putrescine. Tea polyphenols were identified as effective inhibitors for controlling putrescine content in pickled fish.
Editorial Material
Dermatology
Kira Allmeroth, Martin S. Denzel
Summary: Polyamines play a critical role in keratinocyte differentiation, with AMD1 mediating the shift in polyamine ratios. Targeting polyamine availability may be beneficial in treating hyperproliferative skin disorders.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Microbiology
Helene Berthoud, Daniel Wechsler, Stefan Irmler
Summary: Lactic acid bacteria (LAB) play a vital role in food fermentations, but some LAB strains can cause food spoilage. This study discovered that Paucilactobacillus wasatchensis is capable of producing cadaverine and putrescine in both broth and cheese. The researchers identified the genes responsible for this ability and verified the enzymatic activity of two decarboxylases. Acquiring new biochemical data enables more accurate prediction of the potential biogenic amine production in LAB strains and microbiomes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Tao Wu, Jiale Xia, Feng Ge, Hao Qiu, Li Tian, Xiaotian Liu, Rui Liu, Ailiang Jiang, Jing Zhu, Liang Shi, Hanshou Yu, Mingwen Zhao, Ang Ren
Summary: Putrescine (Put) plays a regulatory role in cell growth, and its regulation in fungi, particularly in the biosynthesis of ganoderic acid (GA), involves interactions with ornithine decarboxylase (ODC) proteins and the antizyme (AZ) protein. The target of rapamycin (TOR) can promote Put accumulation and affect GA biosynthesis by inhibiting the level of AZ protein. This study provides insights into the regulatory mechanisms of Put and secondary metabolism in fungi.
MICROBIOLOGY SPECTRUM
(2022)
Article
Oncology
Caleb C. Capellen, Jose Ortega-Rodas, M. Jane Morwitzer, Hadassha M. N. Tofilau, Matthew Dunworth, Robert A. Casero, Surabhi Chandra
Summary: Diabetes can accelerate the progression of certain cancer subtypes, including breast cancer, through the modulation of cellular polyamines. Polyamine inhibitors show promise in suppressing cancer cell proliferation under high glucose conditions, pointing towards a potential therapeutic target for diabetic breast cancer patients.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Article
Multidisciplinary Sciences
Heidi Kaprio, Arafat Siddiqui, Lotta Saustila, Vanina D. Heuser, Maria Gardberg
Summary: The EWSR1::CREM fusion gene, caused by a chromosomal translocation t(10;22)(p11;q12), has been found in various types of cancers with different malignancy levels. The fusion gene leads to production of a chimeric protein, EWSR1::CREM. However, the molecular mechanisms of EWSR1::CREM protein's oncogenic properties have not been systematically characterized. This study used siRNA mediated knockdown to investigate the effects of depletion of EWSR1::CREM protein on gene expression in a melanoma cell line, identified altered pathways and demonstrated the functional differences. The findings contribute to understanding the pathogenesis of tumors with EWSR1::CREM fusion gene and may aid in the development of novel therapeutic strategies.
SCIENTIFIC REPORTS
(2023)
Review
Plant Sciences
Leo Gerlin, Caroline Baroukh, Stephane Genin
Summary: Polyamines play a significant role in the interaction between plants and pathogens, with plant PAs involved in immunity against pathogens by amplifying PTI responses through the production of ROS. Pathogens manipulate plant PAs levels for their own benefit, while also producing PAs themselves during infection.
TRENDS IN PLANT SCIENCE
(2021)
Article
Chemistry, Analytical
Yuze Li, Lixia Jiang, Zhenpeng Wang, Yiran Wang, Xiaohua Cao, Lingwei Meng, Jinghan Fan, Caiqiao Xiong, Zongxiu Nie
Summary: A noninvasive profiling method for bladder cancer diagnosis based on carbonyl metabolic fingerprints using a DSI-MS platform with DMED as a labeling reagent has been developed. This method achieves a high accuracy in distinguishing bladder cancer patients from controls. It may pave the way for patient-friendly point-of-care diagnosis of bladder cancer.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yue Sun, Zhi Zhou, Bing Xie, Zhonghua Wang, Zeper Abliz
Summary: By establishing an AFADESI-MSI system, the molecular information of zebrafish was fully characterized, providing high-quality visual distribution information for over 1000 functional metabolites and mapping a global metabolic network. This comprehensive and in-depth molecular information facilitates the use of zebrafish models in understanding human diseases and developing zebrafish disease models.
ANALYTICAL CHEMISTRY
(2023)
Article
Horticulture
Xiangli Sun, Zebin Yuan, Bo Wang, Liping Zheng, Jianzhong Tan
Summary: This study demonstrated that exogenous putrescine (Put) supplementation can enhance the synthesis of endogenous arginine (Arg) and polyamines (PAs), while reducing the decomposition rate of PAs in Anthurium andraeanum seedlings under chilling stress. On the other hand, supplementation of D-arginine (D-Arg) and/or difluoromethylornithine (DFMO) inhibited the synthesis of endogenous Arg and its conversion to Put, accelerating the decomposition of PAs. Transcriptional analysis confirmed the differential expression of genes involved in these pathways, supporting the observed physiological effects.
SCIENTIA HORTICULTURAE
(2021)
Article
Biochemistry & Molecular Biology
Sivashanmugam Muthukumaran, K. N. Sulochana, Vetrivel Umashankar
Summary: The study focused on designing inhibitory peptides targeting ODC by investigating the interacting interfaces of native ODC forms. Peptide3 and Peptide4 were identified as potentially targeting ODC, showing significant decreases in intracellular polyamine levels, cell adhesion, and cell cycle perturbation in Y79 cells, making them promising therapeutic agents for retinoblastoma.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Plant Sciences
Greta Noelke, Ivana Chudobova, Marcel Houdelet, Daniel Volke, Marcos Lusso, Jesse Frederick, Chengalrayan Kudithipudi, Yanxin Shen, Ujwala Warek, James A. Strickland, Dongmei Xu, Helga Schinkel, Stefan Schillberg
Summary: The unbalanced crosstalk between nicotine and polyamine pathways affects leaf ripening in low-alkaloid tobacco, with transgenic tobacco lines showing delayed leaf maturation and impaired leaf quality. Treatment with polyamine biosynthesis inhibitors decreased polyamine content and ameliorated the phenotype, highlighting the intricate interplay of polyamine and nicotine biosynthesis in tobacco.
Article
Biotechnology & Applied Microbiology
Hari Priya Sivakumar, Sathish Sundararajan, Venkatesh Rajendran, Sathishkumar Ramalingam
Summary: This study identified the distribution of plant ODC and revealed the highly conserved regulatory motifs among diverse plant species. ODC plays an important role in plant metabolic pathways and is crucial for the development of ultra-low nicotine content tobacco lines.
Review
Biotechnology & Applied Microbiology
Jung-Min Choi, Sang-Soo Han, Hak-Sung Kim
BIOTECHNOLOGY ADVANCES
(2015)
Article
Biochemistry & Molecular Biology
Sang Jin Park, Jung Min Choi, Hyun-Ho Kyeong, Song-Gun Kim, Hak-Sung Kim
Article
Biochemistry & Molecular Biology
Da-Eun Hwang, Jung-Min Choi, Chul-Su Yang, Joong-jae Lee, Woosung Heu, Eun-Kyeong Jo, Hak-Sung Kim
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2016)
Article
Chemistry, Physical
Sang-Soo Han, Hyun-Ho Kyeong, Jung Min Choi, Yoo-Kyoung Sohn, Jin-Ho Lee, Hak-Sung Kim
Article
Chemistry, Multidisciplinary
Woosung Heu, Jung Min Choi, Hyun-Ho Kyeong, Yoonjoo Choi, Hee Yeon Kim, Hak-Sung Kim
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2018)
Article
Chemistry, Analytical
Woosung Heu, Jung-Min Choi, Joong-Jae Lee, Sukyo Jeong, Hak-Sung Kim
ANALYTICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Young-Su Jeong, Jung Min Choi, Hyun-Ho Kyeong, Jae-Youl Choi, Eui-Joong Kim, Hak-Sung Kim
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2014)
Article
Cell Biology
Jeong Ho Chang, Jeong Joo Kim, Jung Min Choi, Jung Hoon Lee, Yunje Cho
GENES & DEVELOPMENT
(2008)
Article
Biotechnology & Applied Microbiology
Joong-jae Lee, Hyun Jung Kim, Chul-Su Yang, Hyun-Ho Kyeong, Jung-Min Choi, Da-Eun Hwang, Jae-Min Yuk, Keunwan Park, Yu Jung Kim, Seung-Goo Lee, Dongsup Kim, Eun-Kyeong Jo, Hae-Kap Cheong, Hak-Sung Kim
Article
Biochemistry & Molecular Biology
Eunkyung Kim, Sanghwa Lee, Aram Jeon, Jung Min Choi, Hee-Seung Lee, Sungchul Hohng, Hak-Sung Kim
NATURE CHEMICAL BIOLOGY
(2013)
Article
Multidisciplinary Sciences
Jeong Ho Chang, Yong Hyun Cho, Sun Young Sohn, Jung Min Choi, Ahreum Kim, Young Chang Kim, Sung Key Jang, Yunje Cho
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2009)
Article
Multidisciplinary Sciences
Sung Chun Cho, Moon Cheol Park, Bhumsuk Keam, Jung Min Choi, Yunje Cho, Soonsil Hyun, Sang Chul Park, Junho Lee
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2010)
Article
Multidisciplinary Sciences
Jung Min Choi, Moon-Hyeong Seo, Hyun-Ho Kyeong, Eunkyung Kim, Hak-Sung Kim
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Biochemical Research Methods
Yoonjoo Choi, Sukyo Jeong, Jung-Min Choi, Christian Ndong, Karl E. Griswold, Chris Bailey-Kellogg, Hak-Sung Kim
PLOS COMPUTATIONAL BIOLOGY
(2020)