Article
Engineering, Environmental
Shan-Wei Luo, Adili Alimujiang, Srinivasan Balamurugan, Jian-Wei Zheng, Xiang Wang, Wei-Dong Yang, Jianghu Cui, Hong-Ye Li
Summary: Molybdenum disulfide nanoparticles (MoS2 NPs) have shown potential effects on marine microalgae, stimulating growth and increasing chlorophyll content. They also enhanced protein and carbohydrate content and activated the antioxidant system in response to reactive oxygen species (ROS). Transcriptional analysis showed upregulation of genes related to cellular growth and photosynthesis upon exposure to MoS2 NPs.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Fabian Falkenberg, Leonie Voss, Michael Bott, Johannes Bongaerts, Petra Siegert
Summary: The aim of this study was to characterize four new subtilisins from halotolerant and halophilic microorganisms. The proteases showed activity over a wide pH range and exhibited high stability towards SDS and high concentrations of NaCl. These enzymes demonstrate potential for future biotechnological applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Gabriel Bombo, Nathana L. Cristofoli, Tamara F. Santos, Lisa Schuler, Ines B. Maia, Hugo Pereira, Luisa Barreira, Joao Varela
Summary: Dunaliella viridis TAV01 is a new strain with beneficial features including tolerance to harsh environmental conditions, high growth rates, and amino acid composition suitable for human consumption. Its identification was confirmed at the species level through evolutionary analysis of ITS1 and ITS2 regions, and supported by secondary structure analysis. TAV01 exhibits high protein and lipid contents, predominantly polyunsaturated fatty acids, and a balanced essential amino acid profile. The major carotenoid present is lutein. These qualities make D. viridis TAV01 a potential valuable resource for novel feed or food products.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Medicinal
Ivana Hyrslova, Gabriela Krausova, Iva Mrvikova, Barbora Stankova, Tomas Branyik, Hana Malinska, Martina Huttl, Antonin Kana, Ivo Doskocil
Summary: This study evaluated the effect of Dunaliella salina on the growth and adherence of probiotics, as well as its immunomodulatory and antioxidant properties. The results showed that D. salina promoted the growth and adherence of probiotics, and had immunomodulatory and antioxidant effects, which may help decrease the risk factors of atherosclerosis.
Article
Energy & Fuels
Shamshad Khan, M. Naushad, Jibran Iqbal, Chinna Bathula, Gaurav Sharma
Summary: Biofuel derived from microalgae has become an environmentally friendly alternative to conventional fossil fuels, but there are still technical and economic challenges in mass production and harvesting. This review discusses the difficulties in establishing an integrated system for large-scale microalgal production and harvesting, as well as the advancements in harvesting methods and opportunities for genetic engineering.
Article
Environmental Sciences
Elham Lashani, Hamid Moghimi, Raymond J. Turner, Mohammad Ali Amoozegar
Summary: Selenium oxyanions are released into environments from various activities and are present in wastewater in many locations worldwide. Excessive amounts of selenium have negative impacts on living organisms. Halophilic and halotolerant microorganisms were used for selenium oxyanion remediation in wastewater containing salt. Factors such as aeration, carbon sources, competitive electron acceptors, and reductase inhibitors were investigated for their effects on SeO32- removal. The study also explored the remediation of SeO32- in agricultural effluents containing NO3-. The results showed that aerobic conditions with succinate as a carbon source were most effective for SeO32- removal. Certain ions, such as WO42- and TeO32-, decreased the removal percentage, while SO42- and PO43- had minimal interference. NO3- had an adverse effect on SeO32- biotransformation. Consortia of halophilic/halotolerant bacteria and yeasts were able to reduce SeO32- in synthetic agricultural wastewater by 45-53% within 120 hours. This study suggests that these consortia could be used for the bioremediation of selenium-contaminated drainage water.
ENVIRONMENTAL POLLUTION
(2023)
Article
Multidisciplinary Sciences
Shanling Gan, Shengjia Liang, Qiman Zou, Changhua Shang
Summary: This study optimized the extraction efficiency of carotenoids from Dunaliella parva by determining the most suitable solvent and optimizing the extraction conditions. The results showed a significant improvement in extraction efficiency and provide a basis for future extraction and utilization of carotenoids from Dunaliella parva.
Article
Environmental Sciences
Furkan Orhan, Abdullah Demirci, Sedat Bozari
Summary: This study evaluated the plant growth promoting activities of 15 halophilic/halotolerant bacteria and found that four bacterial strains could enhance wheat growth under salt stress. The ACC deaminase activity of Chromohalobacter sp. and the growth enhancement potential of Oceanobacillus sp. were reported for the first time.
ENVIRONMENTAL ENGINEERING AND MANAGEMENT JOURNAL
(2022)
Review
Biotechnology & Applied Microbiology
Monica Bettina Felix-Castro, Bertha Olivia Arredondo-Vega, Mario Rojas, Gracia Alicia Gomez-Anduro
Summary: This review systematically examines the gene expression of 0-carotene synthesis in Dunaliella and performs a bioinformatic analysis of their promoter region using the available Dunaliella genome sequence. The current research mainly focuses on the first and last steps of 0-carotene synthesis, while other steps like isomerization are limited in Dunaliella. The article also discusses gene expression under different stress conditions, the activation of transcription factor binding sites, and genetic and metabolic engineering approaches to increase 0-carotene synthesis.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Plant Sciences
Javier Perez-Inocencio, Gabriel Iturriaga, Cesar L. Aguirre-Mancilla, Juan Gabriel Ramirez-Pimentel, Maria Soledad Vasquez-Murrieta, Dioselina alvarez-Bernal
Summary: This study analyzed the halophilic and halotolerant bacteria in the root soil of Sesuvium verrucosum and found that these bacteria have the ability to promote plant growth in extreme salinity conditions. They can solubilize phosphorus and zinc, capture sodium, and tolerate high levels of salt stress.
Review
Chemistry, Multidisciplinary
Asma Sarwer, Seham M. Hamed, Ahmed Osman, Farrukh Jamil, Ala'a H. Al-Muhtaseb, Nawaf S. Alhajeri, David W. Rooney
Summary: The depletion of fossil fuels and the continuous increase in carbon dioxide concentrations have led to an energy crisis and environmental issues globally. Microalgal biofuels, produced using sunlight, water, and simple salt minerals, have the potential to be a key solution. Microalgae's high growth rate, photosynthesis, and ability to sequester carbon dioxide make them important biorefinery platforms. Additionally, their ability to produce high-value compounds under environmental stresses makes them a promising alternative to fossil fuels.
ENVIRONMENTAL CHEMISTRY LETTERS
(2022)
Review
Energy & Fuels
Xuan Wang, Yaoli Zhang, Changlei Xia, Ali Alqahtani, Ashutosh Sharma, Arivalagan Pugazhendhi
Summary: Algae biorefinery ideas have recently gained significant attention in the emerging algae mediated bioeconomy, focusing on improving biomass, biofuel, and bioproducts productivity. By understanding the metabolic processes of algae and their potential in generating biofuels and bioproducts, researchers can uncover key obstacles to carbon utilization and photosynthesis potential, leading to increased production efficiency and economic potential. This analysis presents possible mechanisms for a theoretical algae biorefinery structure, addressing specific challenges for each product and exploring market potential for commercialization.
Article
Green & Sustainable Science & Technology
Juan Shi, Yichao Chen, Xiaofeng Liu, Dong Li
Summary: This study isolated a novel halotolerant Pseudomonas aeruginosa M4, which can degrade oil and produce rhamnolipids. By optimizing conditions, the highest oil degradation rate and lipase activity were achieved, and the mechanism of oil degradation and bio-surfactant production process were explored.
JOURNAL OF CLEANER PRODUCTION
(2021)
Review
Green & Sustainable Science & Technology
Jinxiu Wang, Yang Liu, Yecheng Ma, Xinyue Wang, Binglin Zhang, Gaosen Zhang, Ali Bahadur, Tuo Chen, Guangxiu Liu, Wei Zhang, Yidan Zhao
Summary: Halophilic and halotolerant microorganisms have unique structures, physiologies, and genomes that enable them to survive in high salt environments. They play crucial roles in plant stress tolerance, organic pollutant degradation, cultural heritage conservation, and resistance gene spread.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Biotechnology & Applied Microbiology
Furkan Orhan, Ertugrul Ceyran
Summary: This study quantitatively analyzed the presence of four compatible solutes in forty-five halophilic/halotolerant bacterial isolates, and identified several bacterial isolates producing ectoine and hydroxyectoine. The findings of this study provide a basis for the large-scale production of ectoine and hydroxyectoine in the future.
INTERNATIONAL MICROBIOLOGY
(2023)
Review
Physics, Applied
Pietro Ranieri, Nicholas Sponsel, Jon Kizer, Marcela Rojas-Pierce, Ricardo Hernandez, Luciano Gatiboni, Amy Grunden, Katharina Stapelmann
Summary: Plasma agriculture focuses on the role of nonthermal plasma in enhancing plant growth, stress tolerance, and antimicrobial effects. The review discusses key developmental stages of plants, their needs, different growth environments, and the plant microbiome. This provides a foundation for designing plasma-based fertilization strategies to meet the needs of plants and their ecosystem.
PLASMA PROCESSES AND POLYMERS
(2021)
Review
Biotechnology & Applied Microbiology
Dylan M. Brown, Joel Pawlak, Amy M. Grunden
Summary: The pulp and paper industry contains a significant amount of lignocellulosic material that can be converted into valuable chemicals through bacterial fermentation. Bacterial conversion has the potential to enhance the value of lignocellulosic biomass and provide a sustainable and economically feasible approach for the pulp and paper industry.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Greg Buhrman, Paul Enriquez, Lucas Dillard, Hayden Baer, Vivian Truong, Amy M. Grunden, Robert B. Rose
Summary: 2-Oxoglutarate carboxylase (OGC), a member of the biotin-dependent carboxylase family from Aquificales, plays a key role in capturing CO2 for carbon fixation in the reductive tricarboxylic acid cycle, potentially benefiting biofuel production. Structural and functional studies of OGC suggest that substrate binding at high temperatures is facilitated by stabilizing interactions, while biotin plays a rate-limiting role in organizing the active site. Phylogenetic analysis reveals OGC diverged prior to Aquificales evolution and indicates mis-annotations in the Aquificales BC sequences. Additionally, OGC BC dimerization contributes to thermal stability through hydrophilic interactions and structural water molecules.
Article
Biotechnology & Applied Microbiology
Hannah L. Wapshott-Stehli, Amy M. Grunden
Summary: Hydrogen peroxide is a versatile oxidant used in medical and biotechnology industries, with many enzymes requiring it as a reaction mediator. Generating hydrogen peroxide in situ for biocatalysis has several advantages, including avoiding damage to the catalyst and preventing changes in solution parameters. This synthesis cycle benefits the environment by reducing reliance on nonrenewable chemicals from fossil fuels.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Review
Microbiology
Kelly Craig, Brant R. Johnson, Amy Grunden
Summary: Members of the genus Pseudomonas are metabolically versatile and capable of adapting to different environments. Stress physiology of Pseudomonas strains has been extensively studied, revealing various adaptation strategies such as synthesis of protective substances, biofilm formation, and transcription and translation regulation. These survival mechanisms can be beneficial for developing strains with increased viability and product efficacy.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hannah L. Wapshott-Stehli, Amy M. Grunden
Summary: Research has shown that the fatty acid peroxygenase CYP152K6 from Bacillus methanolicus exhibits higher stability at high temperatures compared to other CYP152 enzymes, providing a significant advantage for industrial applications.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Physics, Applied
Hannah L. Wapshott-Stehli, Brayden G. Myers, Maria J. Herrera Quesada, Amy Grunden, Katharina Stapelmann
Summary: Enzymes like OleT(JE) are commonly used in the industry, but the presence of hydrogen peroxide can cause overoxidation. This study explores the use of an atmospheric pressure plasma jet to generate hydrogen peroxide slowly in situ, providing a reliable reaction mediator for OleT(JE) activity.
PLASMA PROCESSES AND POLYMERS
(2022)
Article
Microbiology
Athanasios Zervas, Lea Ellegaard-Jensen, Rosanna C. Hennessy, Frederik Bak, Ying Guan, Courtney Horn Herms, Kitzia Yashvelt Molina Zamudio, Dorthe Thybo Ganzhorn, Dorette Sophie Muller-Stover, Jabeen Ahmad, Amy Grunden, Carsten S. Jacobsen, Mette Haubjerg Nicolaisen
Summary: Understanding the interactions between plants and soil is crucial for improving crop resilience. This study investigates the impact of different rhizocompartments and cultivars on microbial communities.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Microbiology
Jabeen Ahmad, Athanasios Zervas, Lea Ellegaard-Jensen, Rosanna C. Hennessy, Ignazio Carbone, Vicki Cornish, Dorette Sophie Muller-Stover, Amy Grunden, Carsten S. Jacobsen, Mette Haubjerg Nicolaisen
Summary: The study demonstrates differences in microbial communities according to rhizocompartment.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Biotechnology & Applied Microbiology
Juan F. Ortiz-Medina, Mark R. Poole, Amy M. Grunden, Douglas F. Call
Summary: The anode potential has a significant impact on nitrogen gas fixation rates and ammonium production in Geobacter sulfurreducens. Low anode potential results in increased nitrogen fixation activity and higher intracellular concentrations of organic compounds, contributing to improved energy generation and use in microbial electrochemical technologies.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Jason M. Whitham, Amy M. Grunden
Summary: This study evaluated the impacts of JGI trimming and decontamination procedures on assembly and binning metrics, placement of MAGs into species trees, and functional profiles of MAGs extracted from complex rhizosphere metagenomes. Aggressive trimming was found to significantly reduce MAG counts.
CURRENT BIOINFORMATICS
(2023)
Review
Biotechnology & Applied Microbiology
Mara F. Cuebas-Irizarry, Amy M. Grunden
Summary: This review discusses the use of Streptomyces-based technologies in the textile and pulp-milling industry and describes the challenges and recent advances in achieving better biodegradation methods using these microbial catalysts. The main points to be discussed are (1) the application of Streptomyces enzymes in dye decolorization and lignocellulosic degradation, (2) biotechnological processes for textile and pulp and paper waste treatment, and (3) challenges and advances in textile and pulp and paper effluent treatment.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jacob D. Zwilling, Jason Whitham, Franklin Zambrano, Alonzo Pifano, Amy Grunden, Hasan Jameel, Richard Venditti, Ronalds Gonzalez
Summary: Lignocellulosic materials, such as paper, are commonly used for food packaging due to their renewable and biodegradable properties. However, these materials can harbor bacteria during food processing, transportation, and storage, posing a potential risk for foodborne disease outbreaks. This study compared the growth and survivability of Salmonella Typhimurium and Listeria innocua on bleached and unbleached paper packaging materials. The unbleached paper allowed for greater bacterial growth, possibly due to its more hydrophobic nature.
Article
Microbiology
Vijayakumar Somalinga, Emily Foss, Amy M. Grunden
Summary: This manuscript reports the crystal structure and biochemical characterization of a-CA from Photobacterium profundum SS9. The enzyme showed unusual catalytic properties, including maximal activity at low temperatures, retention of activity at acidic pH, and salt-dependent activity under extreme halophilic conditions.
Review
Biology
Deaja Sanders, Amy Grunden, Robert R. Dunn
Summary: Clothing has evolved into an integral part of society over thousands of years, but little is known about the impact wearing clothing has on the human microbiome. This review discusses the history of clothing, microbial persistence and degradation on different fibers, and future opportunities for the industrial and environmental application of clothing microbiology.