期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 98, 期 1, 页码 185-198出版社
SPRINGER
DOI: 10.1007/s00253-013-4829-6
关键词
Biogenic amines; Histamine; Laccase; Lactic acid bacteria; Multicopper oxidase; Putrescine; Tyramine; Wine; Lactobacillus plantarum; Pediococcus acidilactici; Trametes versicolor
资金
- Ministerio de Educacion y Ciencia, Spain [AGL2006-08495, AGL2009-12167]
- ERDF funds
- City Hall of Valencia
The main objectives of this study were the search for enzymatic activities responsible for biogenic amine (BA) degradation in lactic acid bacteria (LAB) strains isolated from wine, their identification, and the evaluation of their applicability for reducing BAs in wine. Fifty-three percent of the 76 LAB cell extracts showed activity against a mixture of histamine, tyramine, and putrescine when analyzed in-gel. The quantification of the degrading ability for each individual amine was tested in a synthetic medium and wine. Most of the bacteria analyzed were able to degrade the three amines in both conditions. The highest percentages of degradation in wine were those of putrescine: up to 41 % diminution in 1 week. Enzymes responsible for amine degradation were isolated and purified from Lactobacillus plantarum J16 and Pediococcus acidilactici CECT 5930 strains and were identified as multicopper oxidases. This is the first report of an efficient BA reduction in wine by LAB. Furthermore, the identity of the enzymes involved has been revealed.
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