期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 97, 期 12, 页码 5627-5634出版社
SPRINGER
DOI: 10.1007/s00253-013-4879-9
关键词
Enzymatic decarboxylation; Acetoacetate decarboxylase; Levulinic acid; 2-Butanone
资金
- Korean Ministry of Knowledge and Economy [2009301009001B]
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [NRF-2009-C1AAA001-0093286]
- Korea Evaluation Institute of Industrial Technology (KEIT) [2009301009001B] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In this study, a novel system for synthesis of 2-butanone from levulinic acid (gamma-keto-acid) via an enzymatic reaction was developed. Acetoacetate decarboxylase (AADC; E.C. 4.1.1.4) from Clostridium acetobutylicum was selected as a biocatalyst for decarboxylation of levulinic acid. The purified recombinant AADC from Escherichia coli successfully converted levulinic acid to 2-butanone with a conversion yield of 8.4-90.3 % depending on the amount of AADC under optimum conditions (30 A degrees C and pH 5.0) despite that acetoacetate, a beta-keto-acid, is a natural substrate of AADC. In order to improve the catalytic efficiency, an AADC-mediator system was tested using methyl viologen, methylene blue, azure B, zinc ion, and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as mediators. Among them, methyl viologen showed the best performance, increasing the conversion yield up to 6.7-fold in comparison to that without methyl viologen. The results in this study are significant in the development of a renewable method for the synthesis of 2-butanone from biomass-derived chemical, levulinic acid, through enzymatic decarboxylation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据