期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 97, 期 22, 页码 9665-9674出版社
SPRINGER
DOI: 10.1007/s00253-013-5199-9
关键词
Scale-up; Oxygen mass transfer; Azospirillum brasilense; Rhizosphere; Corn
资金
- Consejo Nacional de Ciencia y Tecnologia (CONACyT) [178528, CONACYT-INNOVAPYME 181895]
- Programa de Apoyo a Proyectos de Investigacion e Innovacion Tecnologica, Universidad Nacional Autonoma de Mexico [PAPIIT-UNAM IN-210013, IN209113]
Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K (L) a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 x 10(8) CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass.
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