期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 97, 期 6, 页码 2483-2491出版社
SPRINGER
DOI: 10.1007/s00253-012-4232-8
关键词
Dibutyl phthalate (DBP); Transformation; Acinetobacter sp.; DBP hydrolase gene
资金
- National Natural Science Foundation [21107047, 31100087]
- Fundamental Research Funds for Central Universities of The People's Republic of China [1107021154, 1118021114]
- Zhejiang provincial government [Y3100018, 2011C23065]
A dibutyl phthalate (DBP) transforming bacterium, strain M673, was isolated and identified as Acinetobacter sp. This strain could not grow on dialkyl phthalates, including dimethyl, diethyl, dipropyl, dibutyl, dipentyl, dihexyl, di(2-ethylhexyl), di-n-octyl, and dinonyl phthalate, but suspensions of cells could transform these compounds to phthalate via corresponding monoalkyl phthalates. During growth in Luria-Bertani medium, M673 produced the high amounts of non-DBP-induced intracellular hydrolase in the stationary phase. One DBP hydrolase gene containing an open reading frame of 1,095 bp was screened from a genomic library, and its expression product hydrolyzed various dialkyl phthalates to the corresponding monoalkyl phthalates.
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