Article
Biotechnology & Applied Microbiology
Ze-Hui Liu, Zhuo-Fan Deng, Ying Lu, Wei-Huan Fang, Fang He
Summary: Novel multiple antigen delivery system using SpyCatcher/Spytag strategy and self-assembled VLPs formed by porcine circovirus type 2 (PCV2) Cap can significantly elevate immune potency by surface-displaying antigen. This modularly assembled system retains the immune potential of Cap VLP backbone, while enhancing immune potency.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Youngmin Park, Kyungmin Min, Nam Hyung Kim, Ji-hwan Kim, Minhee Park, Hyangju Kang, Eun-Ju Sohn, Sangmin Lee
Summary: This study demonstrated that plant-based expression systems can produce large amounts of proteins, such as recombinant PCV2 capsid protein, for use as subunit vaccine candidates, showing potential for cost-effective vaccines in the pig industry.
Article
Chemistry, Physical
Elvira Tarasova, Noriaki Okimoto, Shanshan Feng, Dmitry Nerukh, Reza Khayat, Makoto Taiji
Summary: The study found that pure PCV2 capsid proteins require acidic conditions to assemble into empty capsids in vitro in the absence of nucleic acids. Molecular dynamics simulation revealed that an appropriate protonation configuration is necessary for driving capsid assembly, with assembly being prohibited at neutral pH due to electrostatic repulsion.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Veterinary Sciences
Wanting Yu, Yuao Sun, Qing He, Chaoying Sun, Tian Dong, Luhua Zhang, Yang Zhan, Naidong Wang, Yi Yang, Yujie Sun
Summary: The mitochondrial localization signal (MLS) in the PCV2 Cap is critical for Cap-induced apoptosis and essential for PCV2 replication, as deletion of the MLS is lethal for virus rescue.
VETERINARY SCIENCES
(2021)
Article
Microbiology
Qizhuang Lv, Tao Wang, Shanchuan Liu, Yulin Zhu
Summary: PCV2 infection inhibits PKR activation through interaction with DNAJC7, helping to understand the immune escape mechanism after PCV2 infection.
VETERINARY MICROBIOLOGY
(2021)
Article
Immunology
Huai-Ying Huang, Benji Brayan Silva, Shen-Pang Tsai, Ching-Yi Tsai, Yu-Chang Tyan, Tzu-Che Lin, Ronilo Jose D. Flores, Kuo-Pin Chuang
Summary: Pigeon circovirus (PiCV) is a common virus in pigeons and utilizing virus-like particles (VLPs) for vaccine development shows promise in combating PiCV. By expressing PiCV capsid protein in HEK-293 cells, self-assembled VLPs induced specific antibodies in pigeons, increased T-cell proliferation and IFN-γ expression, and prevented detectable viral titers in infected pigeons post-vaccination.
Article
Immunology
Wen Zhang, Zhendong Fu, Hongyan Yin, Qingbing Han, Wenhui Fan, Fangkun Wang, Yingli Shang
Summary: PCV2 infection skews macrophages towards M1 status by reprogramming the expression of M1 and M2 associated genes. This skewing is regulated by activation of NF-κB and JNK signaling pathways for M1-associated genes and suppression of M2-associated genes through inhibition of JMJD3 expression. This modulation by PCV2 contributes to bacterial coinfection and viral pathogenesis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Mingxia Sun, Shanghui Wang, Zheng Fang, Man Zhao, Yanfei Gao, Tongqing An, Yabin Tu, Haiwei Wang, Xuehui Cai
Summary: A Sandwich ELISA method based on a monoclonal antibody was developed for the quality control of PCV2 VLPs vaccines with high accuracy.
Article
Veterinary Sciences
Libin Wen, Jiaping Zhu, Fengxi Zhang, Qi Xiao, Jianping Xie, Kongwang He
Summary: This study identified interactions between the Cap protein of porcine circovirus-like virus P1 and five cellular proteins, including Ral GDS, which was confirmed by co-immunoprecipitation. These findings support future investigations into the mechanisms of P1 infection and pathogenesis.
BMC VETERINARY RESEARCH
(2021)
Article
Spectroscopy
Izabel C. V. P. Gogone, Glaucia H. Ferreira, Danielle Gava, Rejane Schaefer, Fabiola F. de Paula-Lopes, Raquel de A. Rocha, Flavia R. O. de Barros
Summary: The study evaluated Raman spectroscopy as a diagnostic tool for porcine parvovirus and porcine circovirus type 2, demonstrating its ability to effectively discriminate virus-infected cells and providing promising bases for future diagnostic applications.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Immunology
Qingping Luo, Waqas Ahmed, Yichen Dai, Ali Mohsin, Haifeng Hang, Yingping Zhuang, Meijin Guo
Summary: The engineered PCV2 truncated capsid subunit Fc-fusion protein induced both cellular and humoral immune responses in mice, showing great potential for applications.
Article
Virology
Agm Rakibuzzaman, Pablo Pineyro, Angela Pillatzki, Sheela Ramamoorthy
Summary: The study showed that vaccination with the suicidal PCV2 vaccine constructs induced strong virus-neutralizing antibody responses, preventing lesions, body-weight loss, and viral replication. The construct was not detectable in vaccinated pigs' sera 14 days post-vaccination, indicating high safety. Exposure to immune selection pressure resulted in mutations to a stop signal, demonstrating both effectiveness and safety in rapidly attenuating PCV2.
Article
Chemistry, Multidisciplinary
Supaporn Klangprapan, Chang-Ching Weng, Wan-Ting Huang, Yaw-Kuen Li, Kiattawee Choowongkomon
Summary: Porcine circovirus type 2 (PCV2) is the main cause of porcine circovirus-associated disease (PCVAD), resulting in significant economic losses for the swine industry globally. In this study, single-chain variable fragments (scFvs) were selected and characterized against the PCV2 capsid using phage display technology. This research demonstrates the potential of scFvs as receptors for biosensor devices in the detection of PCV2 capsid.
Article
Agriculture, Dairy & Animal Science
Xifeng Hu, Zheng Chen, Yu Li, Zhen Ding, Qinghua Zeng, Tong Wan, Huansheng Wu
Summary: An epidemiological investigation in Jiangxi province, China, found that wild boar were exposed to porcine circoviruses (PCVs) including PCV2. The prevalence of PCVs, especially PCV2, highlights the need for preventing contact between livestock and wild boar to reduce the risk of PCV infection. PCV infections are associated with several disorders that have a significant negative economic impact on pig production.
Article
Microbiology
Zhenyu Wang, Jing Chen, Qiong-ge Zhang, Kai Huang, Dan Ma, Qian Du, Dewen Tong, Yong Huang
Summary: PCV2 infection interferes with the activation of type I interferon signaling pathway through its Cap protein and host gC1qR protein, resulting in reduced transcription of IFN-stimulated genes.
VETERINARY MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Pei-Ching Wu, Tzu-Yu Chen, Jiun-Ni Chi, Maw-Sheng Chien, Chienjin Huang
JOURNAL OF BIOTECHNOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Pei-Ching Wu, Shu-Ling Tzeng, Chung-ke Chang, Ya-Fen Kao, Michael J. Waring, Ming-Hon Hou
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Yu-Wen Chen, Roshan Satange, Pei-Ching Wu, Cyong-Ru Jhan, Chung-ke Chang, Kuang-Ren Chung, Michael J. Waring, Sheng-Wei Lin, Li-Ching Hsieh, Ming-Hon Hou
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Multidisciplinary Sciences
Yi-Hwa Lin, Show-Mei Chuang, Pei-Ching Wu, Chun-Liang Chen, Sivakamavalli Jeyachandran, Shou-Chen Lo, Hsu-Shan Huang, Ming-Hon Hou
SCIENTIFIC REPORTS
(2016)
Article
Biochemistry & Molecular Biology
Pin-Hua Wang, Pei-Ching Wu, Richie Huang, Kuang-Ren Chung
MOLECULAR PLANT-MICROBE INTERACTIONS
(2020)
Article
Chemistry, Multidisciplinary
Ching-Ming Chien, Pei-Ching Wu, Roshan Satange, Cheng-Chun Chang, Zi-Lun Lai, Lauren Hagler, Steven C. Zimmerman, Ming-Hon Hou
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Genetics & Heredity
Kuang-Ren Chung, Pei-Ching Wu, Yuh-Kun Chen, Jonar Yago
FUNGAL GENETICS AND BIOLOGY
(2020)
Article
Microbiology
Pei-Ching Wu, Chia-Wen Chen, Celine Yen Ling Choo, Yu-Kun Chen, Jonar I. Yago, Kuang-Ren Chung
MICROBIOLOGICAL RESEARCH
(2020)
Article
Microbiology
Pei-Ching Wu, Chia-Wen Chen, Celine Yen Ling Choo, Yu-Kun Chen, Jonar Yago, Kuang-Ren Chung
Article
Microbiology
Pei-Ching Wu, Yu-Kun Chen, Jonar Yago, Kuang-Ren Chung
Summary: The study reveals that Pex6 affects protein import into peroxisomes in the necrotrophic fungal plant pathogens. It plays crucial roles in the biosynthesis of Woronin bodies, biotin, siderophores, and toxin, as well as the uptake and accumulation of H2O2, growth, and virulence. Furthermore, Pex6 also impacts resistance to specific compounds, underscoring the importance of peroxisomes in physiology, pathogenesis, and development in Alternaria alternata.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Pei-Ching Wu, Celine Yen Ling Choo, Hsin-Yu Lu, Xian-Yong Wei, Yu-Kun Chen, Jonar Yago, Kuang-Ren Chung
Summary: The study demonstrates that Alternaria alternata can resist high levels of reactive oxygen species (ROS) and autophagy plays a protective role in oxidative stress. Mutation of the AaAtg8 gene impairs autophagy, leading to the accumulation of peroxisomes and decreased virulence.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Microbiology
Hsin-Yu Lu, Yu-Ling Huang, Pei-Ching Wu, Xian-Yong Wei, Jonar I. Yago, Kuang- Ren Chung
Summary: The tangerine pathotype of Alternaria alternata can resist high levels of reactive oxygen species (ROS), and a transcription regulator AaSRR1 plays a negative role in ROS resistance. AaSRR1 also regulates conidiogenesis, maintenance of cell wall and membrane integrities, and chitin biosynthesis. Additionally, this study reveals tradeoffs between stress responses and hyphal growth in A. alternata.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Celine Yen Ling Choo, Pei-Ching Wu, Jonar I. Yago, Kuang-Ren Chung
Summary: In this study, the researchers discovered a microbody called peroxisomes that are involved in fatty acid and hydrogen peroxide metabolism in eukaryotes. They found that the AaPex3 gene is responsible for peroxisome biogenesis and resistance to peroxides and superoxide-generating compounds. AaPex3 also affects gene expression related to ROS resistance and is involved in maintaining cell wall integrity and various important functions in the phytopathogenic fungus.
MICROBIOLOGICAL RESEARCH
(2023)
Meeting Abstract
Plant Sciences
H. C. Lin, H. C. Chen, P. C. Wu, K. R. Chung
Article
Chemistry, Multidisciplinary
Wen-Hsuan Tseng, Chung-ke Chang, Pei-Ching Wu, Nien-Jen Hu, Gene-Hsiang Lee, Ching-Cherng Tzeng, Stephen Neidle, Ming-Hon Hou
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)