Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Takeshi Kawahara, Issei Shimizu, Yuuki Tanaka, Keisuke Tobita, Mikado Tomokiyo, Itsuki Watanabe
Summary: This study found that SLPs from Lactobacillus crispatus KT-11 can inhibit human rotavirus infection of intestinal epithelial cells. KT-11 LE showed a dose-dependent suppressive effect on DS-1 infection with pre-infection treatment, while KT-11 SLP concentrate significantly inhibited the proliferative process of DS-1 strain. These results provide crucial evidence for the antiviral effect of SLPs.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Ziyi He, Justin Huffman, Kathrine Curtin, Krista L. Garner, Elizabeth C. Bowdridge, Xiaojun Li, Timothy R. Nurkiewicz, Peng Li
Summary: The cPlate system, enabled by a novel microscale fluid manipulation method, allows for highly scalable multiplexed ELISA analysis with high sensitivity and specificity, shorter assay time, and lower consumption, making it suitable for point-of-care testing in resource-limited regions.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
A-Yeung Jang, Min-Joo Choi, Yong Zhi, Hyun-Jung Ji, Ji-Yun Noh, Jin-Gu Yoon, Hee-Jin Cheong, Woo-Joo Kim, Ho-Seong Seo, Joon-Young Song
Summary: The standardized GBS-ELISA developed in this study shows high specificity and accuracy in evaluating the immunogenicity of GBS vaccines against the three main serotypes. The results demonstrate good performance of the ELISA in assessing the three serotypes, aiding in the development and evaluation of GBS vaccines.
Article
Medicine, General & Internal
Lotfi S. Bin Dahman, Khalid M. Sumaily, Essa M. Sabi, Mohammed A. Hassan, Abeer M. Bin Thalab, Asrar S. Sayad, Saleh M. Bin Kolaib, Fatima M. Alhadhrmi
Summary: This study compared the serum ferritin levels measured by ELISA, Cobas e411, and Cobas Integra 400 methods. The results showed no significant difference between ELISA and Cobas e411, while the values from Cobas Integra 400 were significantly higher. Cobas e411 and Cobas Integra 400 methods demonstrated higher sensitivity, specificity, and accuracy. Further investigations with larger samples are needed to determine interchangeability.
Article
Biochemical Research Methods
Tetsuya Saita, Hiroto Kataoka, Rintaro Sogawa, Tadashi Hayama, Ryoko Tomita, Akira Monji, Yoshito Mizoguchi, Chisato Shimanoe
Summary: The study successfully developed a simple and specific ELISA method for the quantification of PEA, showing comparable results to HPLC measurements and a strong correlation between the values determined by the two methods.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Article
Chemistry, Analytical
Jian Zhou, Jie Liu, Shanshan Song, Liqiang Liu, Chuanlai Xu, Hua Kuang, Wei Ma
Summary: A sensitive and specific monoclonal antibody was developed to detect Diuron in water and sugarcane samples. An immunochromatographic assay strip based on this antibody showed visual detection limits and critical values for Diuron in water and sugarcane samples, which were validated using HPLC-MS.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Applied
Yuechun Li, Han Zhang, Zhaowen Cui, Sijie Liu, Jingke Xu, Conghui Jia, Yaqian Chen, Lulu Wang, Jing Sun, Daohong Zhang, Mingqiang Zhu, Jianlong Wang
Summary: This research explores a novel strategy to enhance the catalytic activity of enzymes, leading to the development of a sensitive ELISA method. By utilizing a chemical staining strategy, the catalytic activity of horseradish peroxidase was directly improved, and a new CS-ELISA method for detecting clenbuterol was established. The CS-ELISA exhibited a 2-fold improvement in sensitivity and linear range compared to conventional ELISA, and demonstrated good applicability in detecting clenbuterol in pork tenderloin samples.
Article
Chemistry, Analytical
Ying Wang, Tong Bu, Yuanyuan Cao, Haiyu Wu, Jia Xi, Qinlin Feng, Chenyu Xuan, Li Wang
Summary: In this study, a peroxidase-mimicking nanozyme-improved enzyme-linked immunosorbent assay (ELISA) was developed using the bimetallic PdRu nanozyme as a catalytic carrier for detecting Escherichia coli O157:H7. The PdRu nanozyme exhibited high catalytic activity and affinity with antibody, ensuring successful establishment of a novel colorimetric biosensor. The PdRu-based ELISA achieved ultrasensitive detection sensitivity and satisfactory specificity and reproducibility, making it suitable for bioassays and clinical diagnostics.
ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Yesenia Castillo, Melissa T. Wardle, Ricardo Gamboa, Mayra Elizalde, Percy Vilchez, Silvia Rodriguez, Robert H. Gilman, Armando E. Gonzalez, Seth E. O'Neal, Hector H. Garcia, Cysticercosis Working Grp Peru
Summary: Coproantigen detection by enzyme-linked immunosorbent assay (coAg ELISA) is a vital tool for detecting and treating cases of Taenia solium taeniasis. However, the assay's procedures require costly materials and sophisticated equipment, which are typically inaccessible in rural settings where the disease is endemic.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Food Science & Technology
Wenwei Lu, Wentao Kong, Pu Yang, Jian Kong
INTERNATIONAL DAIRY JOURNAL
(2015)
Article
Biochemistry & Molecular Biology
Wentao Kong, Venkata S. Kapuganti, Ting Lu
NUCLEIC ACIDS RESEARCH
(2016)
Article
Multidisciplinary Sciences
Chen Liao, Seung-Oh Seo, Venhar Celik, Huaiwei Liu, Wentao Kong, Yi Wang, Hans Blaschek, Yong-Su Jin, Ting Lu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Biotechnology & Applied Microbiology
Shumin Hu, Jian Kong, Wentao Kong, Tingting Guo, Mingjie Ji
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2010)
Article
Biotechnology & Applied Microbiology
Shumin Hu, Jian Kong, Zhilan Sun, Lanlan Han, Wentao Kong, Pu Yang
MICROBIAL CELL FACTORIES
(2011)
Article
Biotechnology & Applied Microbiology
Huaiwei Liu, Yang Wang, Qiang Tang, Wentao Kong, Wook-Jin Chung, Ting Lu
MICROBIAL CELL FACTORIES
(2014)
Article
Microbiology
Yi Xu, Jian Kong, Wentao Kong
Article
Biochemistry & Molecular Biology
Zhilan Sun, Jian Kong, Wentao Kong
MOLECULAR BIOTECHNOLOGY
(2010)
Article
Biochemistry & Molecular Biology
Wenwei Lu, Jian Kong, Wentao Kong
MOLECULAR BIOTECHNOLOGY
(2013)
Article
Microbiology
Zhilan Sun, Lihua Huang, Jian Kong, Shumin Hu, Xiaowei Zhang, Wentao Kong
VETERINARY MICROBIOLOGY
(2012)
Article
Biotechnology & Applied Microbiology
Wentao Kong, Jian Kong, Shumin Hu, Wenwei Lu, Ke Wang, Mingjie Ji
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2011)
Article
Biochemical Research Methods
Wentao Kong, Ting Lu
ACS SYNTHETIC BIOLOGY
(2014)
Article
Multidisciplinary Sciences
Venhar Celik Ozgen, Wentao Kong, Andrew E. Blanchard, Feng Liu, Ting Lu
Article
Biochemistry & Molecular Biology
Wentao Kong, Yuanchao Qian, Philip S. Stewart, Ting Lu
Summary: Synthetic biology has demonstrated the potential to program living microorganisms for various applications. However, current engineering practices mainly focus on planktonic cells, while microbes in nature constantly alternate their lifestyles. This study presents a synthetic genetic program that regulates bacterial life cycle and enables phase-specific gene expression. The program utilizes an engineered protein as the biofilm matrix building block and allows controlled biofilm assembly and decomposition as well as responsive planktonic-biofilm phase transition. By coupling with synthesis modules, it can be programmed for various functional realizations. This work establishes a versatile platform for microbial engineering and gene circuit applications in complex environments.
NATURE CHEMICAL BIOLOGY
(2023)