Article
Biotechnology & Applied Microbiology
Yang-Yang Da, Zi-He Liu, Rui Zhu, Zheng-Jun Li
Summary: This study demonstrates the engineering of E. coli central metabolic pathways to produce pyruvate using a combination of glucose and acetate as carbon sources. By inactivating and deleting specific genes, a strain capable of assimilating both glucose and acetate simultaneously, and eliminating lactate formation, was generated. This engineered strain was able to produce 9.61 g/L pyruvate from 10.27 g/L glucose and 2.00 g/L acetate in shake flask cultivations.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Agricultural Engineering
Xue Sun, Zetao Peng, Chang Li, Yanling Zheng, Ya'nan Cheng, Jianfei Zong, Fuping Lu, Yu Li, Qinggang Li
Summary: In this study, E. coli MG1655 was engineered to achieve high production of 2'-FL. The expression of 2'-FL synthesis pathway genes was finely regulated and branch pathway genes were deleted. The resulting strain produced 46.06 g/L of 2'-FL in a 5-L fermenter. Through adaptive laboratory evolution, a mutation in the rpoC gene was obtained, leading to improved cell tolerance and 2'-FL production of up to 61.06 g/L. This work provides a strategy for improving 2'-FL accumulation and has important implications for constructing high-level 2'-FL producers in the future.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Pengfei Gu, Shuo Zhao, Hao Niu, Chengwei Li, Shuixing Jiang, Hao Zhou, Qiang Li
Summary: A synthetic pathway for producing isobutanol from acetate as the sole carbon source was constructed in E. coli. Overexpression of specific genes and optimization of acetate supplementation concentration resulted in increased isobutanol production.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Daoyi Guo, Xiao Fu, Yue Sun, Xun Li, Hong Pan
Summary: A new biosynthetic pathway was constructed in Escherichia coli for the production of tyrosol acetate and hydroxytyrosol acetate, starting with the production of tyrosol and extending the pathway with the overexpression of alcohol acetyltransferase and hydroxylase enzymes. This study provides a novel approach for biosynthesis of these bioactive compounds with potentially increased bioavailability for various applications in food and cosmetics industries.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Agricultural Engineering
Xun Wang, Xinyi Zhang, Jia Zhang, Longjie Xiao, Yujunjie Zhou, Fei Wang, Xun Li
Summary: A powerful green cell factory has been developed to produce linalool from biodiesel-derived glycerol. Engineered Escherichia coli achieved a linalool titer of 1.07 g/L in flask culture, the highest reported in E. coli to date.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biochemical Research Methods
Meng Liu, Keqin He, Haoran Bi, Meng Wang, Biqiang Chen, Tainwei Tan, Yang Zhang
Summary: The biosynthesis pathway of 2-hydroxyadipate was constructed in Escherichia coli, and 7.11 g/L of 2-hydroxyadipate was produced in the 5 L bioreactor. By using potential optimization strategies through transcriptome analysis, 11.1 g/L of 2-hydroxyadipate was further achieved. These results provide a solid foundation for the biosynthesis of adipic acid and the production of bionylon.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xinrui Dong, Bo Liu, Yihong Bao, Weifeng Liu, Yong Tao
Summary: By co-overexpressing and optimizing the expression of key enzymes involved in violaxanthin biosynthesis, we have developed an efficient Escherichia coli strain capable of producing a large amount of violaxanthin. This is the first report of a metabolically engineered microbial platform that could be used for the commercial production of violaxanthin.
MICROBIAL CELL FACTORIES
(2023)
Article
Agriculture, Multidisciplinary
Yi Zhan, Jun Qiao, Shanshan Chen, Xiaofei Dong, Yuanming Wu, Zhen Wang, Xiaoyuan Wang
Summary: This study aimed to improve the production of colanic acid in E. coli by modifying metabolic pathways. Strategies of deleting genes related to acetate production, O-antigen synthesis, and overexpressing genes involved in lipid carrier synthesis were implemented. The engineered strain achieved significantly higher colanic acid production compared to the control strain.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Hai-De Wang, Jian-Zhong Xu, Wei-Guo Zhang
Summary: In this study, a high L-arginine (L-Arg) producing E. coli strain was obtained through metabolic engineering. The genes speC and speF were found to play important roles in L-Arg accumulation. Feedback inhibition of the L-Arg pathway in E. coli was successfully overcome by combining gene mutations/deletions. Additionally, the insertion of a gene cluster from Corynebacterium glutamicum enhanced the metabolic flux of the L-Arg pathway. The final strain achieved the highest reported level of L-Arg production by E. coli.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Xinwei Yang, Xiaojing Huo, Yaqian Tang, Mingyue Zhao, Yong Tao, Jianzhong Huang, Chongrong Ke
Summary: Based on enzyme evolution and high-throughput screening methods, this study obtained mutants with enhanced GABA productivity. Introduction of the central regulator GadE and enzymes from a biosynthesis pathway resulted in further improvement. In a 5L bioreactor, using crude L-glutamic acid as the substrate, GABA reached a concentration of 307.5 +/- 5.94 g/L with a productivity of 61.49 g/L/h. Therefore, the constructed biocatalyst combined with the whole-cell bioconversion method is an effective approach for industrial GABA production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Sophia Tsouka, Meric Ataman, Tuure Hameri, Ljubisa Miskovic, Vassily Hatzimanikatis
Summary: The Network Response Analysis (NRA) is a novel framework for rational genetic strain design that integrates various analysis methods and constraints, providing a sophisticated alternative to traditional Metabolic Control Analysis (MCA) for metabolic engineering optimization. It allows for the incorporation of physiological data and the generation of multiple alternative optimal strategies given user-defined boundaries and objectives.
METABOLIC ENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Tong Wu, Yumei Liu, Jinsheng Liu, Zhenya Chen, Yi-Xin Huo
Summary: This article comprehensively summarizes the biosynthetic pathways of diols from renewable biomass in Escherichia coli and discusses the metabolic-engineering strategies and dynamic regulation methods to enhance diol production. The challenges in diol biosynthesis and potential methods to improve the diol-producing ability of the host are also proposed.
Article
Biochemical Research Methods
Yu Liu, Xuecong Cen, Dehua Liu, Zhen Chen
Summary: Efforts to improve the production efficiency of (R)-1,3-BDO by Escherichia coli through different metabolic engineering strategies have successfully optimized key pathway enzymes, increased NADPH supply, optimized fermentation conditions, and reduced byproducts formation. The best engineered strain can efficiently produce (R)-1,3-BDO with a yield of 0.6 mol/mol glucose, corresponding to 60% of the theoretical yield. Additionally, the feasibility of aerobically producing 1,3-BDO via a new pathway using 3-hydroxybutyrate as an intermediate has been demonstrated.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Agricultural Engineering
Shunqing Xu, Bin Zhang, Wanhe Chen, Kai Ye, Jian Shen, Pengfu Liu, Jiequn Wu, Hong Wang, Xiaohe Chu
Summary: This article describes the development of a microbial cell factory in Escherichia coli that produces ectoine at high titers, aiming to develop efficient and environmentally friendly bio-based ectoine production technology. The research provides important achievements and potential for industrial applications.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Stefano Camborda, Jan-Niklas Weder, Nadine Toepfer
Summary: CobraMod is a Python package for pathway-centric modification and extension of genome-scale metabolic networks. It integrates data from different metabolic pathway databases and user-curated information, and provides high quality metabolic reconstructions.
Article
Biotechnology & Applied Microbiology
Estefania Sierra-Ibarra, Jorge Alcaraz-Cienfuegos, Alejandra Vargas-Tah, Alberto Rosas-Aburto, Angeles Valdivia-Lopez, Martin G. Hernandez-Luna, Eduardo Vivaldo-Lima, Alfredo Martinez
Summary: In this study, teak wood residues were pretreated, enzymatically saccharified, and detoxified to obtain syrups with high concentrations of fermentable sugars for ethanol production. Two detoxification strategies were tested, which successfully reduced the toxicity of phenolic compounds and increased the ethanol productivity. Over 90% ethanol/sugar yield was achieved in both strategies.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biology
Rosa Santamaria, Patricia Bustos, Jannick Van Cauwenberghe, Victor Gonzalez
Summary: This study investigates the diversity of phages associated with nitrogen-fixing symbiotic Rhizobium species and identifies viral clusters that may represent distinct taxonomic units.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Energy & Fuels
Maria C. Fernandes, Junia Alves-Ferreira, Luis C. Duarte, Helena Pereira, Florbela Carvalheiro, Alfredo Martinez
Summary: The study focused on sequential autohydrolysis, delignification, and fermentation of Rockrose lignocellulosic residues for D-lactic acid production. The results showed increased glucan content and enzymatic digestibility, with a significant positive effect of solid loading and enzyme dosage on glucose production.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Agricultural Engineering
Junia Alves-Ferreira, Florbela Cavalheiro, Luis C. Duarte, Ana R. P. Ferreira, Alfredo Martinez, Helena Pereira, Marcia. C. Fernandez
Summary: In this study, glucan-rich solids and xylose-rich hydrolysates from Cistus ladanifer distillery residues were used for D-lactic acid production. The hydrolysates were subjected to post-hydrolysis under optimized conditions, and subsequent detoxification procedures were performed. The results showed that both hemicellulose- and cellulose-derived sugars can efficiently be used as carbon sources for D-lactic acid production. The study provides insights into optimizing the production of D-lactic acid from distillery residues.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Carlos A. Montenegro-Herrera, Francisco Vera-Lopez Portillo, Georgina T. Hernandez-Chavez, Alfredo Martinez
Summary: New food sources are urgently needed due to population growth. This study investigated the photoautotrophic growth and biochemical composition of Galdieria sulphuraria microalgae strains for potential production of single-cell protein. One strain showed high cell weight and protein content under controlled conditions. The composition of this biomass is suitable for food purposes.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Evelyn Wagner, Estefania Sierra-Ibarra, Natalia L. Rojas, Alfredo Martinez
Summary: In this study, brewery spent grain (BSG) was used as feedstock to produce ethanol using a one-pot bioreactor process. The proposed strategy successfully converted the polysaccharides in BSG into fermentable sugars and achieved high ethanol yields. The results demonstrate the efficient utilization of BSG for bioethanol production.
Article
Biotechnology & Applied Microbiology
Francisco Vera-Lopez Portillo, Estefania Sierra-Ibarra, Rosario Vera-Estrella, Sergio Revah, Octavio T. Ramirez, Luis Caspeta, Alfredo Martinez
Summary: Research found that using glucose under heterotrophic conditions can improve the growth rate, biomass volumetric productivity, and biomass yield of microalgae Galdieria sulphuraria, but inhibits the production of phycocyanin. Using hemicellulosic corn stover hydrolysates may hinder microalgae growth.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Review
Biotechnology & Applied Microbiology
Andres Garcia, Marco T. Fernandez-Sandoval, Daniel Morales-Guzman, Fernando Martinez-Morales, Maria R. Trejo-Hernandez
Summary: In recent years, novel marine bacterial exopolysaccharides (EPS) with various potential applications have been discovered. Marine bacteria rely on EPS to adapt to the extreme environment of the ocean ecosystem. However, high production cost is the major challenge for commercial application.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jose Ignacio Rodriguez-Ochoa, Juan Carlos Fragoso-Jimenez, Noemi Flores, Luz Maria Martinez, Francisco Bolivar, Alfredo Martinez, Guillermo Gosset
Summary: This study investigates the physiological response of Escherichia coli to p-coumaric acid (p-CA), and reveals that p-CA causes cell envelope damage and affects various cellular functions, including leakage of cellular components and synthesis of cell wall.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Alfayuset Ochoa-Chacon, Ana C. Ramos-Valdivia, Hector Mario Poggi-Varaldo, Lourdes Villa-Tanaca, Alfredo Martinez, Teresa Ponce-Noyola
Summary: This study investigated a yeast strain Clavispora lusitaniae CDBB-L-2031 isolated from mezcal must, and found its ability to produce ethanol and xylitol from xylose, cellobiose, and glucose under different culture conditions. The yields of ethanol and xylitol varied depending on the sugar substrates and concentrations.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Daniela Velazquez, Juan-Carlos Sigala, Luz Maria Martinez, Paul Gaytan, Guillermo Gosset, Alvaro R. Lara
Summary: This study selected a mutant strain that mimics Fed-batch behavior in batch cultures through cell engineering and high-throughput screening, and the amount of GFP produced by this strain is significantly higher than that of the W3110 strain in both batch and Fed-batch modes.
MICROBIAL CELL FACTORIES
(2022)
Article
Energy & Fuels
G. R. Pesce, J. Alves-Ferreira, A. Hsiao, I Torrado, A. Martinez, G. Mauromicale, M. C. Fernandes
Summary: This paper assessed the suitability of globe artichoke crop residues for bioethanol production and found that the residual biomass is rich in soluble sugars. The applied strategy of water extraction and dilute acid hydrolysis allowed for the production of bioethanol with a yield of 2399 kg/ha. However, the presence of simple sugars and inhibitors hindered the saccharification process and fermentation efficiency.
BIOENERGY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Estefania Sierra-Ibarra, Alejandra Vargas-Tah, Cessna L. Moss-Acosta, Berenice Trujillo-Martinez, Eliseo R. Molina-Vazquez, Alberto Rosas-Aburto, Angeles Valdivia-Lopez, Martin G. Hernandez-Luna, Eduardo Vivaldo-Lima, Alfredo Martinez
Summary: This study investigated the metabolism of ethanologenic microorganisms for the consumption of sugar mixtures in lignocellulosic hydrolysates, with the aim of improving the fermentation process for the production of bioethanol. The results showed that the rates of glucose and xylose consumption, as well as ethanol production, were influenced by the initial concentrations of glucose and xylose, concentrations of inhibitors, and the positive effect of acetate. The study also highlighted the importance of the xyIR regulator in monosaccharide metabolism and xylose consumption, and confirmed the positive effect of acetate on the co-consumption rates of glucose and xylose.
Article
Biotechnology & Applied Microbiology
Andres Garcia, Liliana Mercado-Munoz, Marco T. Fernandez-Sandoval, Daniel Morales-Guzman, Fernando Martinez-Morales, Maria R. Trejo-Hernandez
Summary: By controlling the oxygen transfer rate (OTRmax) and incubation temperatures, the production and emulsifying activity of bacterial exopolysaccharide produced by the B7H2 strain can be manipulated. Higher OTRmax and incubation temperatures resulted in increased emulsifying activity, while lower temperatures and higher OTRmax promoted cellular growth and exopolysaccharide production.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)