Article
Biochemistry & Molecular Biology
Xin Zhang, Yibing Wang, Jiaqi Liu, Weiyang Wang, Xuecui Yan, Yifa Zhou, Jing Cui, Ye Yuan
Summary: A novel endo-beta-1,6-galactanase with high specificity towards arabinogalactans from plant cell walls was cloned and expressed in this study. The enzyme showed significant activity and could be used for analyzing the fine structure of polysaccharides and preparing bioactive oligosaccharides.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tong Zhou, Yanbo Hu, Xuecui Yan, Jing Cui, Yibing Wang, Feng Luo, Ye Yuan, Zhenxiang Yu, Yifa Zhou
Summary: In this study, a novel exo-beta-1,3-galactanase named PoGal3 was identified and characterized. PoGal3 showed optimal activity at pH 5.0 and 40°C with beta-1,3-galactan as the preferred substrate. It also exhibited enhanced activity in the presence of Zn(2+). This enzyme has potential applications in the construction of glycan blocks for studying biological functions.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Dianyi Li, Zheng Wang, Yong Yu, Huirong Li, Wei Luo, Bo Chen, Guoqing Niu, Haitao Ding
Summary: A novel GH2 beta-galactosidase from Marinomonas sp. BSi20584 was successfully expressed in E. coli and showed stable soluble form. The purified enzyme had a specific activity of 96.827 U mg(-1) at 30 degrees C using ONPG as a substrate. It had an optimum pH of 7.0 and an optimum temperature of 50 degrees C. The enzyme's activity was enhanced by some divalent cations and inhibited by EDTA. Despite being derived from a cold-adapted strain, the enzyme exhibited considerable stability against various physical and chemical elements. It also showed activity towards rare substrates. The enzyme is a promising candidate for the industrial application of beta-galactosidase.
Article
Agriculture, Multidisciplinary
Zhen Qin, Sainan Yu, Kemin Zhang, Xiasen Wei, Junjie Li, Zheyi Zhang, Sibao Wan, Haiyan Gao
Summary: In this study, a GH family 157 endo-beta-1,3-glucanase (BsGlc157A) was identified and characterized from Bacteroides sp. M27. It was found to have applications in fields such as food processing, plant protection, and breweries. The study also revealed its catalytic properties and antifungal activity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Zhen Qin, Sainan Yu, Kemin Zhang, Xiasen Wei, Junjie Li, Zheyi Zhang, Sibao Wan, Haiyan Gao
Summary: In this study, a β-1,3-glucanase named BsGlc157A was identified from Bacteroides sp. M27. It exhibited optimal catalytic activity at pH 6.0 and 40 degrees C. BsGlc157A adopted a classic (beta/alpha)(8) TIM-barrel structure and two catalytic residues were confirmed. Moreover, BsGlc157A showed inhibitory effects on the hyphal growth of fruit pathogenic fungi, highlighting its potential as a biocontrol agent.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Katarina Suchova, Nikolaj Spodsberg, Kristian B. R. Morkeberg Krogh, Peter Biely, Vladimir Puchart
Summary: This study explores the catalytic properties of a GH30_7 xylanase from the fungus Talaromyces leycettanus, demonstrating its specificity towards various types of xylans. The enzyme shows remarkable tolerance towards side-chain substituents, making it attractive for biotechnological applications. Its catalytic diversity reflects the wide range of enzymatic capabilities among eukaryotic GH30_7 xylanases.
Review
Biotechnology & Applied Microbiology
Mauro Mendonca, Mario Barroca, Tony Collins
Summary: This article summarizes 9 glycoside hydrolase families containing enzymes with endo-1,4-8-xylanase activity and discusses their properties, similarities, differences, and biotechnological perspectives. It provides detailed information on substrate specificities, hydrolysis patterns, and the structural determinants of these enzymes, as well as taxonomic aspects of the source organisms. The article highlights shortcomings in current knowledge and identifies research areas that require further clarification. It aims to motivate further research on these enzymes and particularly on the lesser-known endo-1,4-8-xylanase containing families, which will serve as a foundation for the knowledge-based development of process-fitted enzymes in the biobased industries of the future.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Microbiology
Do Young Kim, Jonghoon Kim, Yung Mi Lee, Soo Min Byeon, Jeong Hae Gwak, Jong Suk Lee, Dong-Ha Shin, Ho-Yong Park
Summary: The gene coding for an extracellular endo-beta-1,4-glucanase (GluL) from Antarctic lichen-associated bacterium was identified and expressed in Escherichia coli BL21. The recombinant enzyme showed high specificity and cold-adapted activity, making it a promising candidate for low-temperature processes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Physical
Mariska Thoresen, Samkelo Malgas, Mpho Stephen Mafa, Brett Ivan Pletschke
Summary: This study evaluated and compared the synergistic relationships between cellulases from different microbial sources, finding that not all endo-to-exo-cellulase interactions are synergistic. Maximizing compatibility between exo- and endo-cellulases is critical for designing improved synergistic cellulose-degrading cocktails for industrial-scale biomass degradation.
Article
Biochemistry & Molecular Biology
Anders Lonstrup Hansen, Johanna M. Koivisto, Signe Simonsen, Zehui Dong, Ramon Crehuet, Dennis K. Hansen, Martin Willemoes
Summary: The study identified the importance of Asp-682 and a conserved water molecule in the activation of the catalytic nucleophile in endo-alpha-N-acetylgalactosaminidase EngBF. Mutagenesis experiments showed that His-685, His-718, and the catalytic water molecule are also crucial for catalytic activity.
Article
Chemistry, Applied
Guanchen Liu, Jingjing Shen, Yaoguang Chang, Xuanwei Mei, Guangning Chen, Yuying Zhang, Changhu Xue
Summary: A new bacterial enzyme, Fun174A, was discovered to degrade sulfated fucans in sea cucumbers, exhibiting significant biological and biomedical activities.
CARBOHYDRATE POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Shumao Chai, Xueliang Zhang, Yuejiao Gao, Lishuang Nie, Chenchen Xiang, Mingming Cao, Shaochen Wang, Zhiyang Feng
Summary: A novel endo-/β-1,4-glucanase ZFYN1413-87 with strong enzyme activity was identified and studied in this research, showing 52% retained activity even after treatment in pH 3.0 buffer for 2 hours, representing a unique example in the GH6 family.
CARBOHYDRATE RESEARCH
(2021)
Article
Chemistry, Physical
Jorick Franceus, Jolien Lormans, Lore Cools, Matthias D'hooghe, Tom Desmet
Summary: The study demonstrates significant phosphorylase activity can be established in GH3 glycoside hydrolases through single-site substitutions and further optimized through mutational paths. These findings suggest new insights into developing phosphorylases and provide a novel approach for future research in this field.
Article
Biotechnology & Applied Microbiology
Sebastian J. Muderspach, Folmer Fredslund, Verena Volf, Jens-Christian Navarro Poulsen, Thomas H. Blicher, Mads Hartvig Clausen, Kim Krighaar Rasmussen, Kristian B. R. M. Krogh, Kenneth Jensen, Leila Lo Leggio
Summary: This study characterized the most thermophilic endo-beta-1,4-galactanase known to date and successfully engineered it to modify the degradation profile while maintaining its desirable thermostability. The length of oligosaccharide products is an important property for both industrial and natural GHs.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biochemistry & Molecular Biology
Xiao-Bao Sun, De-Ying Gao, Jia-Wen Cao, Yu Liu, Zhou-Ting Rong, Jia-Kun Wang, Qian Wang
Summary: We report a novel AA10 LPMO enzyme derived from Bacillus subtilis (BsLPMO10A) with an extensive substrate specificity, especially for polysaccharides linked via 8-1,4 glycosidic bonds. Through synergistic action with various glycoside hydrolases, BsLPMO10A dramatically accelerates the saccharification of glucan, xylan, cellulose, and chitin. This study provides a promising candidate for lignocellulosic biomass biorefinery.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Tomoya Kitakaze, Miki Yoshikawa, Yasuyuki Kobayashi, Naohiro Kimura, Naoki Goshima, Takahiro Ishikawa, Yoshiyuki Ogata, Yoko Yamashita, Hitoshi Ashida, Naoki Harada, Ryoichi Yamaji
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2020)
Article
Multidisciplinary Sciences
Naoki Harada, Masayuki Nomura, Yasuhiro Yoda, Shigenobu Matsumura, Hiroshi Inui, Ryoichi Yamaji
Article
Biochemistry & Molecular Biology
Tomohiro Bito, Mariko Bito, Tomomi Hirooka, Naho Okamoto, Naoki Harada, Ryoichi Yamaji, Yoshihisa Nakano, Hiroshi Inui, Fumio Watanabe
Article
Nutrition & Dietetics
Mari Noguchi, Tomoya Kitakaze, Yasuyuki Kobayashi, Katsuyuki Mukai, Naoki Harada, Ryoichi Yamaji
Article
Substance Abuse
Yoshihisa Takahashi, Shiori Watabe, Arisa Togashi-Kumagai, Masato Watanabe, Erdenetsogt Dungubat, Hiroyuki Kusano, Yasuyuki Kobayashi, Naoki Harada, Ryoichi Yamaji, Keiichiro Sugimoto, Toshio Fukusato
Article
Gastroenterology & Hepatology
Naoki Harada, Yukari Minami, Kazuki Hanada, Ryo Hanaoka, Yasuyuki Kobayashi, Takeshi Izawa, Takashi Sato, Shigeaki Kato, Hiroshi Inui, Ryoichi Yamaji
Article
Nutrition & Dietetics
Erdenetsogt Dungubat, Shiori Watabe, Arisa Togashi-Kumagai, Masato Watanabe, Yasuyuki Kobayashi, Naoki Harada, Ryoichi Yamaji, Toshio Fukusato, Galtsog Lodon, Badamjav Sevjid, Yoshihisa Takahashi
Article
Biochemistry & Molecular Biology
Naoki Harada, Yumi Arahori, Mai Okuyama, Paula B. Luis, Akil Joseph, Tomoya Kitakaze, Naoki Goshima, Claus Schneider, Hiroshi Inui, Ryoichi Yamaji
Summary: This study identifies the activation of G protein-coupled receptor 97 (GPR97) by curcumin and investigates its molecular mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Naoki Harada, Keitaro Kubo, Teruaki Onishi, Tomoya Kitakaze, Tsuyoshi Goto, Hiroshi Inui, Ryoichi Yamaji
Summary: This study found that males have lower body temperature compared to females, and the androgen-androgen receptor (AR) signaling suppresses adrenergic receptor-mediated thermogenesis, providing an explanation for sexual differences in body temperature.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Erdenetsogt Dungubat, Hiroyuki Kusano, Ichiro Mori, Hirosuke Tawara, Mitsuko Sutoh, Naoki Ohkura, Masakatsu Takanashi, Masahiko Kuroda, Naoki Harada, Emiko Udo, Masakazu Souda, Bungo Furusato, Toshio Fukusato, Yoshihisa Takahashi
Summary: According to this study, sex differences in non-alcoholic fatty liver disease (NAFLD) were observed in Tsumura Suzuki obese diabetes (TSOD) and db/db mice, which may serve as suitable models for studying the sex differences in this disease.
Article
Biochemistry & Molecular Biology
Yasuyuki Kobayashi, Mai Kubota, Keiichiro Sugimoto, Tomoya Kitakaze, Naoki Harada, Ryoichi Yamaji
Summary: The study found that housing mice in small cages leads to obesity and abdominal fat accumulation, but dietary oleamide can suppress this obesity.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Naoki Harada, Mai Okuyama, Yoshiaki Teraoka, Yumi Arahori, Yoh Shinmori, Hiroko Horiuchi, Paula B. Luis, Akil Joseph, Tomoya Kitakaze, Shigenobu Matsumura, Tohru Hira, Norio Yamamoto, Takashi Iuni, Naoki Goshima, Claus Schneider, Hiroshi Inui, Ryoichi Yamaji
Summary: The identification of molecular targets of bioactive food components is important for understanding their physiological functions. It has been discovered that curcumin activates GPR55, indicating its involvement in the physiological function of curcumin. The F190 residue of GPR55 is crucial for the interaction with curcumin.
NPJ SCIENCE OF FOOD
(2022)
Article
Nutrition & Dietetics
Yasuyuki Kobayashi, Natsumi Watanabe, Tomoya Kitakaze, Keiichiro Sugimoto, Takeshi Izawa, Kenji Kai, Naoki Harada, Ryoichi Yamaji
Summary: The study showed that dietary oleamide can rescue skeletal muscle atrophy in mice, possibly by activating the PI3K/Akt/mTOR signaling pathway and restoring autophagy flux.
BRITISH JOURNAL OF NUTRITION
(2021)
Article
Chemistry, Medicinal
Takuya Yamane, Momoko Imai, Satoshi Handa, Naoki Harada, Ryoichi Yamaji, Tatsuji Sakamoto, Tetsuo Ishida, Hiroshi Inui, Takenori Nakagaki, Yoshihisa Nakano
Article
Food Science & Technology
Misa Ogushi, Momoko Imai, Minako Yoshizawa, Kengo Suzuki, Naoki Harada
FOOD SCIENCE AND TECHNOLOGY RESEARCH
(2020)