期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 90, 期 6, 页码 1933-1942出版社
SPRINGER
DOI: 10.1007/s00253-011-3182-x
关键词
Bifunctional; Metagenomic; Xylanase; Endoglucanase
资金
- Chinese High-tech Research and Development Program [2007AA021302]
A new gene, RuCelA, encoding a bifunctional xylanase/endoglucanase, was cloned from a metagenomic library of yak rumen microorganisms. RuCelA showed activity against xylan and carboxymethylcellulose (CMC), suggesting bifunctional xylanase/endoglucanase activity. The optimal conditions for xylanase and endoglucanase activities were 65A degrees C, pH 7.0 and 50A degrees C, pH 5.0, respectively. In addition, the presence of Co+ and Co2+ can greatly improve RuCelA's endoglucanase activity, while inhibits its xylanase activity. Further examination of substrate preference showed a higher activity against barley glucan and lichenin than against xylan and CMC. Using xylan and barley glucan as substrates, RuCelA displayed obvious synergistic effects with beta-1,4-xylosidase and beta-1,4-glucosidase. Generation of soluble oligosaccharides from lignocellulose is the key step in bioethanol production, and it is greatly notable that RuCelA can produce xylo-oligosaccharides and cello-oligosaccharides in the continuous saccharification of pretreated rice straw, which can be further degraded into fermentable sugars. Therefore, the bifunctional RuCelA distinguishes itself as an ideal candidate for industrial applications.
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