Article
Chemistry, Analytical
Evrim Elcin, Ferhan Ayaydin, Huseyin Avni Oktem
Summary: Formaldehyde, a hazardous carcinogenic volatile organic compound, can be detected by whole-cell biosensors. A cell bioreporter system based on a DNA damage response gene was developed for sensitive detection of formaldehyde in air.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Hongrui Chen, Xinyue Mu, Jian Li, Yuqi Qin, Liqiang Yan
Summary: A new method for detecting SDS was developed by preparing a cationic fluorescent probe, which enables quantitative determination of SDS with high selectivity and sensitivity in the range of 5-50 μM through the formation of banded assemblies showing red fluorescence.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Weiying Lin, Zhiming Gou, Aijing Wang, Xiaomei Zhang, Yujing Zuo
Summary: In this study, two types of multi-head cationic siloxanes were designed for PFOS detection and further used for exploring the interaction between surfactant and dye. The study revealed the possible mechanism of high selectivity in PFOS detection through the fluorescence behavior comparison of different cationic molecules.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Mengying Deng, Jing Yuan, Haibin Yang, Xuli Wu, Xiaoyuan Wei, Yang Du, Garry Wong, Yuyong Tao, Gang Liu, Zongwen Jin, Jun Chu
Summary: This study introduced a bright green fluorescent protein, Clover4, obtained through structure-guided mutagenesis, which showed improved performance in LUMABS sensors for antibody detection in serum. The Clover4-LUMABS sensors exhibited a larger dynamic range and lower limit of detection compared to previous sensors, offering a promising approach for fast and highly sensitive antibody detection.
Article
Biochemistry & Molecular Biology
Edit Szabo, Anna Kulin, Balint Jezso, Nora Kucsma, Balazs Sarkadi, Gyorgy Varady
Summary: The ABCB1 multidrug transporter is important in the absorption, distribution, metabolism, and elimination of pharmaceutical compounds. This study demonstrates that TP dyes can be used as sensitive and selective markers to examine the function and properties of ABCB1, as well as efficiently select and separate Pgp-expressing intact cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Xinwei Zhang, Jiao Ma, Kaide Ou, Xin Cong, Binbin Zhang, Qi Zhou, Yonggui Liao, Yajiang Yang, Hong Wang
Summary: A novel type of vesicles (VT/G) composed of Gemini cationic surfactant (G12-8-12) and TPE-2OH, an aggregation-induced emission (AIE) fluorescence probe, were prepared. The vesicles showed good binding and bactericidal effects on bacteria due to the compression of the interlayer and the generation of active substances. This new strategy has potential applications in bacteria detection and killing.
Article
Chemistry, Physical
Gabriela Stinga, Adriana Baran, Alina Iovescu, Florina Branzoi, Dan-Florin Anghel
Summary: In this study, attractive smart and emissive nanomaterials were designed by embedding methylene blue in complexes using a supramolecular host-guest strategy. The photophysical properties of the assemblies were highlighted, and the formation of different complexes at varying surfactant concentrations was analyzed. The results suggest potential applications in chemical sensing and imaging.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Review
Chemistry, Analytical
Namdoo Kim, Seunghan Shin, Se Won Bae
Summary: cAMP serves a crucial role in signal transduction pathways, with research focusing on its dynamics leading to insights for drug development and disease treatment. To enable real-time non-invasive imaging, genetically-encoded sensors based on fluorescent proteins and luciferases have been developed as powerful tools.
Article
Chemistry, Analytical
Lina Wu, Liuqin Su, Minfang Deng, Xinyi Hong, Mingkai Wu, Miaomiao Zhang, Emmanuelle Bouveret, Xiaomei Yan
Summary: Quantitative measurement of protein-protein interactions (PPIs) remains challenging, but a new method using CRISPR/Cas9 technology to insert fluorescent protein tdTomato and 12-amino acid tetracysteine tag shows promise in simplifying this process. This method allows for rapid and high-throughput analysis of PPIs at the single-cell level and has been demonstrated to be effective in ranking binding affinities, mapping residues involved in PPI, and screening protein interaction inhibitors.
Article
Chemistry, Physical
Ramesh Kumar Banjare, Manoj Kumar Banjare, Kamalakanta Behera, Mamta Tandon, Siddharth Pandey, Kallol K. Ghosh
Summary: In this study, the micellization behavior of two conventional cationic surfactants in aqueous DESs solutions was investigated using various techniques, showing significant changes in micellar properties in the presence of DESs. Additionally, the interaction of human serum albumin with cationic surfactant micellar solutions in DESs was explored, revealing a stronger binding affinity between albumin protein and DES-surfactant systems. These findings may have implications for potential applications in colloids, protein biophysics, and nanoscience.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Biochemistry & Molecular Biology
Anna A. Deeva, Albert E. Lisitsa, Lev A. Sukovatyi, Tatiana N. Melnik, Valentina A. Kratasyuk, Elena Nemtseva
Summary: In order to understand the adaptation mechanisms of enzymes to a constantly changing environment, we investigated the effects of temperature variation on the structure and function of luciferases from Photobacterium leiognathi and Vibrio harveyi. The results showed that the activity and stability of the enzymes vary at different temperatures, and the presence of sucrose can increase the stability of the enzymes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
David Duran, Patricia Bernal, David Vazquez-Arias, Esther Blanco-Romero, Daniel Garrido-Sanz, Miguel Redondo-Nieto, Rafael Rivilla, Marta Martin
Summary: The genome of Pseudomonas fluorescens F113 encodes three putative type VI secretion systems (T6SS) - F1-, F2- and F3-T6SS. Bioinformatic analysis revealed that these systems are similar to those in Pseudomonas aeruginosa. RNA-Seq analysis showed that F1- and F3-T6SS are active under all conditions, while F2-T6SS did not show relevant expression in the tested conditions.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Danil S. Serdyukov, Tatiana N. Goryachkovskaya, Irina A. Mescheryakova, Sergei A. Kuznetsov, Vasiliy M. Popik, Sergey E. Peltek
Summary: A fluorescent biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz (THz) radiation was developed by transforming Escherichia coli cells with plasmid containing the tdcR gene promotor controlling the expression of yellow fluorescent protein TurboYFP. The biosensor's fluorescence threshold and dynamics were found to be dependent on irradiation conditions, with heat shock or chemical stress resulting in absence of fluorescence induction. It is applicable for studying the influence of THz radiation on the tdcR gene promotor activity involved in threonine and serine transport and metabolism in E. coli.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Chemistry, Analytical
Juanzu Liu, Leping Lin, Peiyu Yao, Wei Zhao, Jiao Hu, Xue-Hui Shi, Shiwu Zhang, Xiaobo Zhu, Dai-Wen Pang, An-An Liu
Summary: Bacterial infectious diseases are common and pose a serious threat to human health. Current methods for detection lack specificity. In this study, ultrabright fluorescent nanospheres were used as reporters to develop an immunochromatographic dyad test strip for early detection and distinction of different stages of bacterial infections. By monitoring the levels of C-reactive protein and heparin-binding protein in plasma samples, the strip can rapidly and accurately diagnose and assess bacterial infections.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Simon Kriegler, Michel W. Jaworek, Rosario Oliva, Roland Winter
Summary: The helical structure of cationic antimicrobial peptides (cAMPs) plays a crucial role in their activity when interacting with membranes. We discovered that high hydrostatic pressure not only increases the likelihood of cAMPs adopting a helical conformation in bacterial lipid bilayer membranes and bulk solution but also affects bacterial membranes even at pressures up to 10 kbar. This suggests that high-pressure treatment could enhance cAMP activity in high-pressure food processing to extend food shelf-life.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Xi Wu, Chong Zhang, Xin-Hui Xing, Zhenyu Yun, Lin Zhao, Qi Wu
Summary: In order to enhance the efficiency of oxidized cofactor recycling for the production of aromatic chiral alcohols, a study was conducted to design and construct four novel bifunctional fusion proteins combining ADH and NOX enzymes. The fusion proteins showed improved specific activities for both ADH and NOX moieties, with decreased affinities towards cofactors. Furthermore, the fusion proteins displayed varying effects on the recycling efficiency of the oxidized cofactor during enantioselective oxidation, suggesting potential applications in the biosynthesis of chiral alcohols.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Yikang Zhou, Yaomeng Yuan, Yinan Wu, Lu Li, Aysha Jameel, Xin-Hui Xing, Chong Zhang
Summary: The article introduces a new workflow for constructing and characterizing a large number of biosensors using DNA trackable assembly method and FACS-seq technology. With this workflow, a malonyl-CoA biosensor with the largest dynamic response range was successfully obtained and the genotype-phenotype relationships of uncharacterized combinations were predicted using machine learning algorithms. The workflow is of great significance for the design, tuning, and profiling of biosensor response curves.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xinyu Yu, Xiuxia Liu, Xiong Gao, Xunxun Luo, Yankun Yang, Ye Li, Chunli Liu, Chong Zhang, Zhonghu Bai
Summary: This study presents a novel recombinant protein manufacturing platform that utilizes ethanol as both a delayed carbon source and an inducer. By improving the inducible promoter and optimizing transcriptional engineering, this platform achieves superior protein expression in Corynebacterium glutamicum. Ethanol metabolism is found to be required for the upregulation of the improved promoter. The platform successfully produces the first secretory overexpression of the de novo design protein NEO-2/15.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Dong He, Yifan Xing, Yi Wang, Wen Zeng, Wei Gao, Nan Su, Chong Zhang, Haihong Chen, Xin-Hui Xing
Summary: This study demonstrates that covalent modification of wheat gluten hydrolysate with chlorogenic acid improves its functional properties, including thermal stability, emulsifying and foaming capabilities, and antioxidant activity.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Shuang Li, Xi-hao Liao, Xin-yu Yu, Aysha Jameel, Xin-Hui Xing, Zheng-lian Xue, Chong Zhang
Summary: A novel high-throughput screening technology, fluorescence-activated droplet sorting (FADS), combined with a genetically encoded fluorescent biosensor, iGluSnFR, was successfully used to screen variants with high glutaminase production. The strategy significantly improved the screening efficiency and reduced reagent consumption, facilitating the directed evolution of industrial enzymes and cell factories.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Microbiology
Yujie Meng, Shuang Li, Chong Zhang, Hao Zheng
Summary: By using droplet-based microfluidic cultivation, researchers encapsulated and cultivated individual bacterial cells from the honeybee gut, leading to interesting findings. The cultivation technique revealed higher strain-level diversity compared to gut samples, and comparative genomic analysis provided insights into host adaptations.
Article
Food Science & Technology
Dong He, Wen Zeng, Yi Wang, Yifan Xing, Kang Xiong, Nan Su, Chong Zhang, Yuan Lu, Xinhui Xing
Summary: Ulcerative colitis (UC), an incurable and complex digestive disease, affects millions of people worldwide. This study investigated the potential of bioactive peptides as functional foods or drugs for UC prevention and treatment, with a focus on alcohol-soluble components from fermentation broth by fresh wheat germ and apple (AC-WGAF). Four novel peptides were identified from AC-WGAF, and peptide T24 exhibited the most significant anti-colitis activity. T24 prevented tight junction protein loss, maintained epithelial barrier integrity, and promoted cell proliferation by regulating mitogen-activated protein kinase signaling pathways. Overall, T24 shows promise as a functional food or novel drug for UC prevention and treatment.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Biotechnology & Applied Microbiology
Ruirui Li, Xiaojie Guo, Kang Xiong, Xinhui Xing, Chong Zhang
Summary: A novel micro-tube bioreactor (MTB) based on a high gas-permeability Teflon tube was reported, providing controllable and bubble-free oxygen supply with low shear stress during microbial cultivation. The MTB demonstrated improved growth performance compared to conventional shake flasks, including higher incipient growth rate and terminal biomass accumulation. The MTB also enabled high-density microbial cultivation, as evidenced by a tripled biomass accumulation in a fed-batch cultivation of Bacillus subtilis compared to batch mode. Moreover, adaptive evolution against oxygen for Lactobacillus plantarum showed remarkable improvement in growth performance, increasing biomass accumulation by 130-150%, indicating the MTB's potential as a platform for microbial cultivation and adaptive evolution.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Xingjin Jian, Xiaojie Guo, Zhengshuo Cai, Longfeng Wei, Liyan Wang, Xin-hui Xing, Chong Zhang
Summary: A novel integrated platform, the single-cell microliter-droplet screening system (MISS Cell), was developed for automated, high-throughput microbial monoclonal colony cultivation and picking. Compared with solid plates, the MISS Cell generated a larger number of monoclonal colonies with higher initial growth rates using fewer resources. The MISS Cell can serve as a universal platform for efficient monoclonal colony production in high-throughput applications.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemical Research Methods
Lan-Yu Cui, Jing Yang, Wei-Fan Liang, Song Yang, Chong Zhang, Xin-Hui Xing
Summary: By adding 10 mmol L-1 sodium formate in the mevalonic acid (MEV) accumulating stage (at 72 h), Methylobacterium extorquens AM1 (AM1) achieved a MEV yield of 0.067 gMEV/g methanol, which is the highest reported yield to date. Sodium formate had global effects on metabolic pathways, significantly increasing the ratios of reducing equivalents, enhancing the metabolic rate of pathways demanding reducing cofactors, and redirecting carbon flux to MEV synthesis. Coupling formate to methanol-based production provides a promising way for converting C1 substances to useful chemical products.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Engineering, Environmental
Chenqi Niu, Chong Zhang, Juewen Liu
Summary: This article focuses on the development of new aptamers for estrogenic compounds and their application in the detection of E2 in wastewater. The newly selected aptamers have the advantages of small size and high affinities, and two of them were successfully converted into fluorescent biosensors with low limit of detection.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xinyu Yu, Shuang Li, Huibao Feng, Xihao Liao, Xin-Hui Xing, Zhonghu Bai, Xiuxia Liu, Chong Zhang
Summary: This study developed a CRISPRi-microfluidics screening platform to identify crucial gene targets for enhancing r-protein secretion in Corynebacterium glutamicum. By screening 46,549 single-guide RNAs, unknown targets related to transmembrane transport, amino-acid metabolism, and redox regulation were discovered. These findings were used to construct a hyperproducer for multiple proteins through combinatorial engineering of redox-response transcription factors. The CRISPRi technology and FlAsH-based screening platform used in this study have broad applications for other microbial species and recombinant proteins.
METABOLIC ENGINEERING
(2023)
Letter
Biology
Xihao Liao, Changge Guan, Chenghua Wang, Xinjie Chen, Xiaomei Lin, Lianghua Lu, Lu Li, Ting Wang, Nan Su, Bian Wu, Xinquan Wang, Chong Zhang, Yi Wang, Yuan Lu, Xin-Hui Xing
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
Chenqi Niu, Juewen Liu, Xinhui Xing, Chong Zhang
Summary: In this study, a novel strategy was proposed to eliminate the non-specific signal from non-specific amplification by integrating the CRISPR-Cas12a system into two-templates exponential amplification reactions (EXPAR). The developed EXPAR-Cas12a strategy named EXPCas acted as a filter to filter out non-specific amplificons and improved the signal-to-background ratio significantly. With miRNA-21 as the target, the detection could be accomplished in 40 min with a LOD of 103 fM and no non-specific amplification was observed.
ANALYTICA CHIMICA ACTA
(2023)
Article
Agriculture, Multidisciplinary
Hai-Hong Chen, Wei Li, Yi Wang, Bing Xu, Xi Hu, Xiao-Bing Li, Jun-Yu Liu, Chong Zhang, Can-Yang Zhang, Xin-Hui Xing
Summary: Through multi-omics technology, 16 potential DPP-IV-inhibiting peptides were identified in hemp seeds. These peptides exhibited strong DPP-IV binding affinity and efficiently inhibited DPP-IV activity, leading to increased insulin and glucagon-like peptide-1 concentrations. This study provides an effective method for the development of food-derived therapeutic DPP-IV-inhibiting peptides.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)