Article
Chemistry, Multidisciplinary
Hao Dong, Wenxue Zhang, Shengmin Zhou, Hanjie Ying, Ping Wang
Summary: This work presents a rational design of biofilm catalysts that integrate extra- and intracellular catalysis systems with optimized substrate channeling to achieve efficient multistep biosynthesis. The results demonstrate that the engineered biofilm catalysts have great potential in integrating extra- and intracellular catalysis and can act as sustainable supports for whole-cell catalysis.
Article
Green & Sustainable Science & Technology
Mariana Valdez Castillo, Satinder Kaur Brar, Sonia Arriaga, Jean-Francois Blais, Antonio Avalos Ramirez
Summary: This study aimed to increase the production of 2-phenylethanol by fermenting whey with immobilized yeasts. The results showed that the immobilization of yeasts on inert support significantly increased the production of 2-phenylethanol, with the highest yield achieved using filter Kaldnes plastic rings.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Agricultural Engineering
Jorg Toepel, Rohan Karande, Bruno Buehler, Katja Buehler, Andreas Schmid
Summary: Hydrogen (H2) is a promising fuel for climate neutral energy carriers, and photosynthesis-driven H2 production, which relies on sunlight and water, has shown potential. Research has been conducted using cyanobacteria as biocatalysts in capillary biofilm photobioreactors (CBRs). The study found that biofilm formation and stability are influenced by light and CO2 availability, and H2 production rates vary between different cyanobacteria species. By adjusting light and O2 levels, biofilm formation and H2 production could be enhanced, with high stability lasting for weeks. Final biocatalyst titers reached up to 100 g l-1, and H2 production rates were up to 300 mu mol H2 l-1h-1 and 3 mu mol H2 gcdw-1h-1 in biofilms.
BIORESOURCE TECHNOLOGY
(2023)
Review
Environmental Sciences
Duowei Lu, Hao Bai, Fangong Kong, Steven N. Liss, Baoqiang Liao
Summary: Membrane aerated biofilm bioreactor (MABR) technology has shown significant progress in wastewater treatment, with advantages of high oxygen transfer, COD/N removal, and energy efficiency. Future research directions include biofilm thickness control, new application potentials, and design of new membrane materials and modules.
CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2021)
Review
Energy & Fuels
Vasundhara Jain, Aditi Awasthi, Sanjoy Ghosh
Summary: Xylitol is a value-added polyalcohol widely used in various industries. While chemical technology is currently the main method for commercial xylitol production, biotechnological synthesis from lignocellulosic biomass offers a promising and sustainable alternative. This review focuses on recent advancements in xylitol bioproduction using immobilization methods, which improve biocatalyst reusability and stability, decrease process costs, and are essential for large-scale production.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Microbiology
Maria Hijosa-Valsero, Ana I. Paniagua-Garcia, Rebeca Diez-Antolinez
Summary: This study demonstrates the feasibility of cell immobilization in erythritol production for the first time. The use of immobilized cells resulted in similar erythritol concentration and yield compared to free cells, indicating the efficiency of the immobilization system as well as cost reduction in reagents and energy.
Article
Chemistry, Physical
Wen Xuan Woo, Hau Sern Koh, Jian Ping Tan, Swee Keong Yeap, Peer Mohamed Abdul, Abdullah Amru Indera Luthfi, Shareena Fairuz Abdul Manaf
Summary: The article highlights the significance and challenges of biohydrogen production from lignocellulosic biomass. The use of immobilization techniques can overcome the current critical bottlenecks and improve commercial competitiveness.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Biotechnology & Applied Microbiology
Minami Ogawa, Pablo Carmona-Jimenez, Teresa Garcia-Martinez, Jesus Valentin Jorrin-Novo, Juan Moreno, Maria Dolores Rey, Jaime Moreno-Garcia
Summary: Immobilized cell technologies (ICT) are commonly used in brewing to improve cell stability, flexibility, and control. Yeast biocapsules, a new form of ICT, were found to be more suitable for cell reutilization than alginate beads but had a greater impact on beer clarity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Jorge Rojas Lopez-Menchero, Minami Ogawa, Juan C. Mauricio, Juan Moreno, Jaime Moreno-Garcia
Summary: The alginate coating effectively prevents cell leakage from yeast biocapsules but may hinder alcoholic fermentation. In grape must fermentations, it does not affect wine acidity but impacts concentrations of some volatile compounds.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Environmental Sciences
Jing Zhao, Gaofeng Ni, Maria Piculell, Jie Li, Zhetai Hu, Zhiyao Wang, Jianhua Guo, Zhiguo Yuan, Min Zheng, Shihu Hu
Summary: This study investigated the establishment, structure, and microbial community composition of biofilms in three laboratory-scale moving bed biofilm reactors, and found that the depth of living space significantly influenced the biofilm formation and community composition.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Review
Biotechnology & Applied Microbiology
Pascal S. Leonov, Xavier Flores-Alsina, Krist V. Gernaey, Claus Sternberg
Summary: Harnessing the potential of biofilm fermentations for converting renewable biomass into value-added products has shown promise in improving productivity and product yields, as well as enhancing resistance to hazardous environments. However, challenges such as inconsistent fermentation performance, slow reactor start-up, and insufficient process monitoring still hinder the commercialization of this technology. A multidisciplinary approach combining genetic engineering, specialized biofilm reactor development, model-based process control, and mixed microbial biofilm technology may be crucial for overcoming these challenges and realizing the full potential of biofilm fermentations.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Elza R. Mikheeva, Inna V. Katraeva, Andrey A. Kovalev, Dmitriy A. Kovalev, Alla N. Nozhevnikova, Vladimir Panchenko, Ugo Fiore, Yuri V. Litti
Summary: This study focused on continuous production of biohydrogen from cheese whey, utilizing heat and acid pretreatments to optimize the process. The highest hydrogen yields and production rates were obtained with specific conditions such as hydraulic retention time and organic load rate. Firmicutes were dominant at the phylum level, while Lactobacillus and Bifidobacterium were dominant at the genus level.
APPLIED SCIENCES-BASEL
(2021)
Article
Engineering, Environmental
Navid Ahmadi, Mona Abbasi, Ali Torabian, Mark C. M. van Loosdrecht, Joel Ducoste
Summary: The transformation of four pharmaceuticals in a moving bed biofilm reactor was investigated under different COD/N ratios. Changes in the COD/N ratio influenced the competition between heterotrophic and nitrifying communities, resulting in shifts in microbial populations and composition in the biofilms. Autotrophic conditions exhibited higher transformation rates, indicating the importance of nitrification.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Review
Environmental Sciences
Shabnam Murshid, AdithyaJoseph Antonysamy, GnanaPrakash Dhakshinamoorthy, Arun Jayaseelan, Arivalagan Pugazhendhi
Summary: Industrialization plays a significant role in a nation's growth, but it also contributes to environmental degradation. Pollution, including aquatic, terrestrial, and airborne pollution, has a profound impact on the environment, and the increase in industries and population exacerbates this issue. The biological technique, specifically biofilm technology, is an efficient and viable approach without major downsides.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Agricultural Engineering
Zhen-Yu Wu, Juan Xu, Lan Wu, Bing-Jie Ni
Summary: Three-dimensional biofilm electrode reactors (3D-BERs) are highly efficient in refractory wastewater treatment, utilizing filled particle electrodes as both electrodes and microbial carriers. The synergy between electricity and microorganisms is emphasized, along with a systematic summary of electrode materials, including critical particle electrodes. The configurations of 3D-BERs, integration with wastewater treatment reactors, and adaptation to various types of wastewater are discussed, highlighting the prospects and challenges for future research and application.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Attia Iram, Deniz Cekmecelioglu, Ali Demirci
Summary: This study evaluated the effects of different salts, peptone, and yeast extract on enzyme secretion in Aspergillus niger strains and optimized the nitrogen source for maximum enzyme production. The results showed that yeast extract had a positive effect on cellulase production, while ammonium sulfate positively affected both cellulase and xylanase production. Optimizing the nitrogen content significantly increased enzyme production.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Luis J. Bastarrachea, David W. Britt, Robert E. Ward, Ali Demirci
Summary: The modification of polypropylene with chitosan was found to enhance the generation of Lactobacillus casei biofilms and their lactic acid production. Biofilms grown in rich media produced significantly more lactic acid, while the chitosan-modified polypropylene harbored more cells compared to the control. The deposition of nutrients and biomass on the chitosan-modified polypropylene was confirmed to be higher, with thicker biofilms observed when grown in rich media.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Hetian Hu, Jeffrey M. Catchmark, Ali Demirci
Summary: The addition of pullulan significantly improved the mechanical properties and production of BC nanocomposites, while co-culture fermentation further enhanced the properties of BC.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Review
Biotechnology & Applied Microbiology
Shin-Ping Lin, Darin Khumsupan, Yu-Jou Chou, Kuan-Chen Hsieh, Hsien-Yi Hsu, Yuwen Ting, Kuan-Chen Cheng
Summary: Atmospheric cold plasma is a nonthermal technology that is widely used in engineering and biotechnology. Despite facing challenges, it has the potential to impact operation processes, enhance safety, and reduce costs, while also providing opportunities for further research.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Yen-Tso Lai, Chih-Yao Hou, Shin-Ping Lin, Yi-Chen Lo, Chien-Hao Chen, Chang-Wei Hsieh, Hui-Wen Lin, Kuan-Chen Cheng
Summary: This study investigated nontraditional inoculation strategies using aroma-producing yeast strains for Kyoho wine fermentation and conducted instrumental analysis and sensory evaluation. The results showed that mixed culture could promote the formation of esters and glycerol and reduce the content of ethanol in wine. Sensory analysis suggested that the sequential inoculation treatment of three yeast strains was associated with floral, red fruity, and tropical fruity aroma attributes.
JOURNAL OF FOOD SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Fu-Chiun Hsu, Wan-Ting Lin, Kuan-Chen Hsieh, Kuan-Chen Cheng, James Swi-Bea Wu, Yuwen Ting
Summary: Cold argon plasma jet treatment has been shown to reduce the immunoreactivity of peanut protein, providing a potential non-thermal technique to mitigate peanut allergenicity in the food industry.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Food Science & Technology
Chen-Che Hsieh, Shu-Han Yu, Kai-Wen Cheng, Yu-Wei Liou, Cheng-Chih Hsu, Chang-Wei Hsieh, Chia-Hung Kuo, Kuan-Chen Cheng
Summary: The study used fresh fourth-day Chenopodium formosanum sprouts as the substrate for Rhizopus oligosporus fermentation. The resultant products had higher antioxidant capacity compared to C. formosanum grains. Fermentation in a bioreactor (BF) led to higher free peptide content and enzyme activity than traditional plate fermentation (PF), as well as the discovery of over twenty new metabolites.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Energy & Fuels
Attia Iram, Deniz Cekmecelioglu, Ali Demirci
Summary: This study compares the treated DDGS samples to common carbon sources and shows that acid-treated DDGS is a significantly better carbon source for cellulase and xylanase production. Two strains of Aspergillus niger showed the highest enzyme activity levels. The results confirm the significant positive effect of dilute acid treatment in making DDGS a feasible feedstock for hydrolytic enzyme production.
Review
Biochemistry & Molecular Biology
Darin Khumsupan, Shin-Ping Lin, Chang-Wei Hsieh, Shella Permatasari Santoso, Yu-Jou Chou, Kuan-Chen Hsieh, Hui-Wen Lin, Yuwen Ting, Kuan-Chen Cheng
Summary: The cost-effectiveness and efficiency of atmospheric cold plasma (ACP) technology has driven extensive research in its applications within the food industry. It has been found to be effective in food disinfection, modification, drying pre-treatment, nutrient extraction, active packaging, and food waste processing. Reactive oxygen and nitrogen species (RONS) play crucial roles in achieving the desired outcomes. This review article discusses the operations of ACP and summarizes its recent progress in food processing and safety, while also proposing current challenges and future prospects.
Review
Engineering, Chemical
Attia Iram, Ali Ozcan, Irfan Turhan, Ali Demirci
Summary: Humankind has been utilizing food fermentations unknowingly since the creation of bread and cheese. Microbial fermentation has been extensively used for production of commodity chemicals and natural food ingredients. This review summarizes the prominent food ingredients and novel fermentation food products currently being produced via microbial fermentation, along with strategies to enhance such processes and the potential of economical feedstocks for value-added products.
Article
Biotechnology & Applied Microbiology
Yen-Wenn Liu, Chi-Mei Liu, Hung-Yueh Chen, Darin Khumsupan, Hsien-Yi Hsu, Hui-Wen Lin, Chang-Wei Hsieh, Kuan-Chen Cheng
Summary: Ganoderma formosanum, an endemic medicinal mushroom in Taiwan, exhibited significant reduction of melanin activity through ethyl acetate extraction of its mycelium, which inhibits tyrosinase. Optimization of G. formosanum mycelium production with anti-melanogenic activity was conducted by investigating different variables (carbon sources and concentrations, initial pH value, and temperature). The optimal conditions involved the use of 50 g/L lactose as the carbon source at pH 7 and 25 degrees C for 9 days. Subsequently, the G. formosanum ethanolic extracts-optimized (GFE-O) were found to effectively reduce intracellular melanin content in B16-F10 melanoma cells and induce hypopigmentation in zebrafish embryo in a non-toxic manner.
FERMENTATION-BASEL
(2023)
Article
Food Science & Technology
Kuan-Chen Hsieh, Kuan-Chen Cheng, Ting-Jang Lu, Yu-Jou Chou, Yuwen Ting
Summary: In this study, the effectiveness of two plasma treatment gases, air and nitrogen, on reducing peanut allergen Ara h 1 was investigated. The results showed that after 13 minutes of cold plasma treatment, the antigenicity of Ara h 1 decreased by approximately 55%. Nitrogen plasma treatment caused less oxidative damage to the samples compared to air plasma treatment. Overall, both air and nitrogen plasmas were effective in reducing Ara h 1, but nitrogen cold plasma preserved the original quality of the peanut samples better. This study demonstrated the potential of nitrogen cold plasma for effective food processing in reducing Ara h 1.
Article
Biochemistry & Molecular Biology
Ting-Yun Lin, Yun-Ting Wu, Hui-Ju Chang, Chun-Chen Huang, Kuan-Chen Cheng, Hsien-Yi Hsu, Chang-Wei Hsieh
Summary: This study evaluated the antioxidative and anti-inflammatory activities of polysaccharides extracted from unripe Carica papaya L. (papaya) fruit. The PP-3 fraction showed the highest antioxidant activity and significantly reduced oxidative stress and inflammation in human epidermal keratinocytes. PP-3 has the potential to be used as a biofunctional ingredient in the cosmetic industry.
Review
Biotechnology & Applied Microbiology
Attia Iram, Deniz Cekmecelioglu, Ali Demirci
Summary: First-generation bioethanol byproduct DDGS is rich in fiber and nutrients, used for enzyme production in second-generation bioethanol. Pretreatment methods release fermentable sugars for microbial growth and enzyme production, with optimization leading to enhanced enzyme production for efficient 2G bioethanol production.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Chih-Yao Hou, Chen-Che Hsieh, Ying-Chi Huang, Chia-Hung Kuo, Min-Hung Chen, Chang-Wei Hsieh, Kuan-Chen Cheng
Summary: This study aimed to develop fermented dairy products using appropriate processing technology and probiotics, combined with Taiwan djulis and high-quality milk sources. The fermentation process was optimized to enhance the nutrients and active ingredients in the fermented milk. This functional fermented dairy product can be developed into high-value agricultural products and applied in medical and therapeutic fields.
FERMENTATION-BASEL
(2022)
