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Chemistry, Multidisciplinary
Hasan DeMirci, Yashas Rao, Gabriele M. Stoffel, Bastian Vogeli, Kristina Schell, Aharon Gomez, Alexander Batyuk, Cornelius Gati, Raymond G. Sierra, Mark S. Hunter, E. Han Dao, Halil Ciftci, Brandon Hayes, Fredric Poitevin, Po-Nan Li, Manat Kaur, Kensuke Tono, David Adrian Saez, Samuel Deutsch, Yasuo Yoshikuni, Helmut Grubmueller, Tobias J. Erb, Esteban Vohringer-Martinez, Soichi Wakatsuki
Summary: This study used various experimental methods to investigate the structural organization of ECR in Kitasatospora setae, and found that its catalytic activity is carried out by a pair of dimers. The results reveal the dynamic organization and synchronized inter- and intrasubunit communications of this enzyme during catalysis.
ACS CENTRAL SCIENCE
(2022)
Article
Chemistry, Physical
Rodrigo Recabarren, Matthias Tinzl, David Adrian Saez, Aharon Gomez, Tobias J. Erb, Esteban Vohringer-Martinez
Summary: Researchers used computer simulations and high-level ab initio calculations to trace the free energy changes along two possible reaction paths, revealing the microscopic mechanism of nature's most efficient CO2-fixing enzyme in storing reactive enolate species as a strategy.
Article
Biotechnology & Applied Microbiology
Katharina Kremer, Doreen Meier, Lisa Theis, Stephanie Miller, Aerin Rost-Nasshan, Yadanar T. Naing, Jan Zarzycki, Nicole Paczia, Javier Serrania, Patrick Blumenkamp, Alexander Goesmann, Anke Becker, Martin Thanbichler, Georg K. A. Hochberg, Michael S. Carter, Tobias J. Erb
Summary: Carbon metabolism is crucial for providing energy and building blocks for organisms. Understanding the mechanisms of metabolic control in bacteria is important for applications in health and biotechnology. This study focuses on functional degeneracy, a well-known phenomenon in bacteria where the same carbon source can be used through different metabolic routes. We demonstrate that two seemingly degenerate central carbon metabolic pathways in P. denitrificans are linked with each other, allowing coordinated switch between them during growth. Our findings elucidate the molecular basis of metabolic plasticity in central carbon metabolism, improving our understanding of flux partitioning between anabolism and catabolism in bacterial metabolism.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Biology
Ekaterina Petushkova, Ekaterina Mayorova, Anatoly Tsygankov
Summary: Purple non-sulfur bacteria are promising producers of biofuels, capable of utilizing organic waste waters to produce valuable compounds. Understanding their anaplerotic pathways is crucial for utilizing them as potential biofuel producers.
Review
Biochemical Research Methods
Iria Bernhardsgruetter, Gabriele M. M. Stoffel, Tarryn E. Miller, Tobias J. Erb
Summary: Utilizing carboxylases to capture and convert CO2 is crucial for realizing a circular, carbon-neutral economy; interest in utilizing and engineering carboxylases has been steadily growing in recent years; pioneering efforts in the field have paved the way for a diverse CO2-fixation biochemistry that can find application in various industries.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xin Wu, Yongbo Yu, Tonghui Huang
Summary: A potential ACC inhibitor was identified in this study, and a series of related compounds were synthesized for investigation, among which 6g showed the strongest ACC inhibitory activity, while 6l exhibited potent cytotoxicity. Preliminary mechanistic studies suggested that these compounds may act by reducing malonyl-CoA levels, arresting the cell cycle, and inducing apoptosis.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Article
Agricultural Engineering
Juhi Gupta, Rashmi Rathour, Neha Maheshwari, Indu Shekhar Thakur
Summary: The study demonstrated the enhanced production capability of strain Cupriavidus sp. ISTL7 for polyhydroxyalkanoates using acetate and glucose, with a need for optimization of solvent extraction and energy consumption for sustainable production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Applied
Tao Jiang, Fumin Wang, Xubin Zhang, Yi Zhai, Guojun Lv, Yu Shen, Yuzhou Wu
Summary: In this study, controlled synthesis of ZSM-5 mesocrystals was achieved without special additives, utilizing selected area electron diffraction and fast Fourier transform to demonstrate the well oriented feature of synthesized zeolites. The crystallization process was further analyzed, with oriented particle attachment identified as an effective mechanism guiding the synthesis of mesocrystals. Superior catalytic performance was demonstrated in aldol condensation reactions, with 93% conversion obtained between benzaldehyde and 2-hydroxyacetophenone.
MICROPOROUS AND MESOPOROUS MATERIALS
(2021)
Article
Microbiology
Karola Schuhle, Martin Saft, Bastian Voegeli, Tobias J. Erb, Johann Heider
Summary: The novel acyl-CoA dehydrogenase IaaF identified in Aromatoleum aromaticum is involved in the degradation of the auxin indoleacetate, showing high catalytic efficiency, enantiospecificity, and unique reactivity.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Masato Ikeda, Keisuke Takahashi, Tatsunori Ohtake, Ryosuke Imoto, Haruka Kawakami, Mikiro Hayashi, Seiki Takeno
Summary: Recent research found a unique mechanism of lipid homeostasis in Corynebacterium glutamicum, involving a futile cycle of acyl-CoA hydrolysis and resynthesis mediated by acyl-CoA thioesterase (Tes) and acyl-CoA synthetase (FadD). Engineering this cycle in a high-fatty-acid producer significantly increases production, offering a useful strategy for improving fatty acid production in this industrially important microorganism.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Claudia Durall, Kateryna Kukil, Jeffrey A. Hawkes, Alessia Albergati, Peter Lindblad, Pia Lindberg
Summary: This study successfully introduced the glyoxylate shunt into Synechocystis PCC 6803 and found that complete expression of the glyoxylate shunt can increase succinate accumulation. Supplementation with succinate dehydrogenase enzyme inhibitor and acetate can further enhance succinate accumulation in the presence of nitrate.
MICROBIAL CELL FACTORIES
(2021)
Article
Microbiology
Li Liu, Philip C. Brown, Martin Koenneke, Harald Huber, Simone Koenig, Ivan A. Berg
Summary: The autotrophic HP/HB cycle functions differently in thermoacidophilic and ammonia-oxidizing archaea, with the latter being more energy-efficient than the former. The enzymes responsible for the reactions in these cycles were shown to have evolved independently in these two archaeal lineages.
Article
Biochemistry & Molecular Biology
Megan D. Smith, Lambros J. Tassoulas, Troy A. Biernath, Jack E. Richman, Kelly G. Aukema, Lawrence P. Wackett
Summary: OleA enzyme catalyzes non-decarboxylative Claisen condensation reaction to initiate the biosynthesis of membrane hydrocarbons and beta-lactone natural products. The reaction mechanism of OleA was probed using p-nitrophenyl esters, incorporating them into Claisen condensation products for the first time.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Dragan Miscevic, Ju-Yi Mao, Teshager Kefale, Daryoush Abedi, Murray Moo-Young, C. Perry Chou
Summary: This study describes the development of a microbial bioprocess usingEscherichia colito produce high levels of 5-aminolevulinic acid (5-ALA). Through the implementation of the Shemin pathway and CRISPRi interference, metabolic engineering strategies were applied to direct carbon flux and enhance the biosynthesis of 5-ALA, achieving significant production yields.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Multidisciplinary Sciences
Franziska R. Traube, Dilara Ozdemir, Hanife Sahin, Constanze Scheel, Andrea F. Glueck, Anna S. Geserich, Sabine Oganesian, Sarantos Kostidis, Katharina Iwan, Rene Rahimoff, Grazia Giorgio, Markus Mueller, Fabio Spada, Martin Biel, Juergen Cox, Martin Giera, Stylianos Michalakis, Thomas Carell
Summary: Tet3 in neurons interacts with the mitochondrial enzyme glutamate dehydrogenase to produce alpha KG in the nucleus, impacting epigenetic changes during neural plasticity.
NATURE COMMUNICATIONS
(2021)