期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 87, 期 2, 页码 781-786出版社
SPRINGER
DOI: 10.1007/s00253-010-2572-9
关键词
Phytoremediation; Methylmercury pollution; Transgenic tobacco; Polyphosphate; merB gene; Mercury accumulation
资金
- Ministry of the Environment, Japan
To develop the potential of plant for phytoremediation of methylmercury pollution, a genetically engineered tobacco plant that coexpresses organomercurial lyase (MerB) with the ppk-specified polyphosphate (polyP) and merT-encoding mercury transporter was constructed by integrating a bacterial merB gene into ppk/merT-transgenic tobacco. A large number of independent transgenic tobaccos was obtained, in some of which the merB gene was stably integrated in the plant genome and substantially translated to the expected MerB enzyme in the transgenic tobacco. The ppk/merT/merB-transgenic tobacco callus showed more resistance to methylmercury (CH(3)Hg(+)) and accumulated more mercury from CH(3)Hg(+)-containing medium than the ppk/merT-transgenic and wild-type progenitors. These results suggest that the MerB enzyme encoded by merB degraded the incorporated CH(3)Hg(+) to Hg(2+), which then accumulated as a less toxic Hg-polyP complex in the tobacco cells. Phytoremediation of CH(3)Hg(+) and Hg(2+) in the environment with this engineered ppk/merT/merB-transgenic plant, which prevents the release mercury vapor (Hg(0)) into the atmosphere in addition to generating potentially recyclable mercury-rich plant residues, is believed to be more acceptable to the public than other competing technologies, including phytovolatilization.
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