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Biotechnology & Applied Microbiology
Carlos Eduardo de Oliveira Nascimento, Lorena Caixeta de Oliveira Simoes, Josiani de Cassia Pereira, Ronivaldo Rodrigues da Silva, Evandro Antonio de Lima, Gabriel Cimonetti de Almeida, Ana Lucia Barretto Penna, Mauricio Boscolo, Eleni Gomes, Roberto da Silva
Summary: This study produced Xylooligosaccharides (XOs) by extracting xylan from sugarcane bagasse and hydrolyzing it using an enzyme. The XOs stimulated the growth of beneficial intestinal bacteria and could be used as a supplement to promote the growth of probiotic strains.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wei Xia, Mengkai Hu, Yang Pan, Dan Wu, Jing Wu
Summary: A novel dual-plasmid system was successfully constructed to increase the production efficiency of recombinant proteins by combining genomic integration and episomal expression strategies. Results showed that the highest enzyme yield was achieved by simultaneously transforming the host strain with integrated P-GAP and episomal P-GCW14. This novel expression system could be suitable for the production of proteins related to the food and pharmaceutical industries, without the risk of methanol toxicity.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ting Miao, Abdul Basit, Junquan Liu, Fengzhen Zheng, Kashif Rahim, Huiqiang Lou, Wei Jiang
Summary: The study improved the production of Myceliophthora thermophila xylanase through signal peptide engineering, resulting in enhanced enzyme activity. Further analysis showed that Mtxyn11C was more effective than commercial xylanase in degrading xylan, and when combined with other xylanolytic enzymes, it improved the hydrolysis efficiency of xylan.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Zhenyang Liu, Sitao Wen, Guogan Wu, Huawei Wu
Summary: A new glycoside hydrolase family 10 (GH10) xylanase gene, ArXyn10c20, from anaerobic rumen microorganism Anaeromyces robustus, was successfully synthesized and expressed. The recombinant xylanase showed optimal pH and temperature of 5.5 and 40 degrees Celsius, respectively. It exhibited stability in a pH range of 5.0-9.0 and its function was enhanced by the addition of 1 mM Cu2+. The hydrolysis products of ArXyn10c20 on beechwood xylan were xylobiose, xylotriose, and xylotetraose. Additionally, when added to bread making, ArXyn10c20 significantly improved dough and bread quality.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Katesuda Aiewviriyasakul, Benjarat Bunterngsook, Hataikarn Lekakarn, Wipawee Sritusnee, Pattanop Kanokratana, Verawat Champreda
Summary: The study developed an endo-beta-1,4-xylanase with high specificity for producing prebiotic xylooligosaccharides, operating optimally at moderate temperatures to reduce energy costs in production processes. The enzyme showed potential for XOS production and lignocellulosic biomass saccharification, working best at 45 degrees Celsius and pH 6.0 with specific cleavage patterns releasing xylobiose as the major product.
BIOTECHNOLOGY LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Lin Cui, Huoqing Huang, Honglian Zhang, Xiaolu Wang, Xing Qin, Tao Tu, Jie Zhang, Xiaoyun Su, Huimin Yu, Yingguo Bai, Huiying Luo, Bin Yao, Yuan Wang
Summary: A novel fusion protein expression strategy was proposed to increase the expression level of hen egg white lysozyme (HEWL). The results showed that the fusion protein expressed using this strategy had higher expression level and activity compared to HEWL alone.
Article
Biotechnology & Applied Microbiology
Sitao Wen, Guogan Wu, Huawei Wu
Summary: The newly discovered xylanase ArXyn10 from Anaeromyces robustus was cloned and expressed in Pichia pastoris, showing optimal activity at pH 5.5 and 40°C. ArXyn10 exhibited good stability and was able to increase reducing sugar content in bread making, improving the quality of bread.
Review
Biotechnology & Applied Microbiology
Vladimir Puchart, Katarina Suchova, Peter Biely
Summary: This article discusses the importance and specificity of xylanases in bioconversion, focusing mainly on the substrate and product specificities of GH30 xylanases and structure-activity relationship, as well as exploring their biotechnological potential.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemistry & Molecular Biology
Lorenzo Briganti, Caio Capetti, Vanessa O. A. Pellegrini, Silvina Ghio, Eleonora Campos, Alessandro S. Nascimento, Igor Polikarpov
Summary: The study demonstrates the structural characteristics of PxXyn11B in the GH11 family and the important role of SBS in its activity and conformational mobility, revealing that SBS regulates the conformational modulation of GH11 enzymes through a positive molecular feedback loop.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Patricia Gomez de Santos, Manh Dat Hoang, Jan Kiebist, Harald Kellner, Rene Ullrich, Katrin Scheibner, Martin Hofrichter, Christiane Liers, Miguel Alcalde
Summary: In this study, two unusual acidic peroxygenases were successfully produced at a large scale in yeasts, with expression levels boosted to 290mg/liter through signal peptide modification and mutagenesis. These highly secreted, active, and stable peroxygenases have a broad pH activity profile, making them promising tools for future engineering endeavors and direct industrial application.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yoshihito Arai, Yunako Shingu, Hisashi Yagi, Hirokazu Suzuki, Takashi Ohshiro
Summary: Fucoidans, a type of polysaccharides found in brown algae, have been extensively studied for their biological activities. In this study, a microbial strain capable of utilizing fucoidan as a sole carbon source was isolated and identified. Genomic analysis revealed the presence of two genes encoding fucoidanases in this strain. These enzymes exhibited different specificities for fucoidans from different sources. This finding highlights the potential of a single microbial strain to produce multiple types of fucoidanases.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Biochemical Research Methods
Wang Ying, Wang YaPing, Huang Can, Ma Lixin, Yan Hong, Min Yong, Liu Xiaoyan, Rao Ben
Summary: Methyl parathion hydrolase (MPH) was efficiently and specifically produced from Plesiomonas sp. M6 using a Pichia pastoris multi-copy expression system. Increasing the number of MPH gene copies enhanced the production of recombinant MPH. The addition of Ni2+, Co2+, and Mg2+ as metal ions significantly increased MPH-R activity. Immobilised MPH-R showed improved stability and activity compared to free MPH-R, demonstrating its potential for industrial applications.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Article
Microbiology
Melvin Chun Yun Tan, Muhammad Ramziuddin Zakaria, Kok Jun Liew, Chun Shiong Chong
Summary: Hahella genus is poorly studied with limited identified species. This study isolated Hahella sp. CR1 from mangrove soil in Tanjung Piai National Park, Malaysia and sequenced its whole genome. The CR1 strain showed high similarity with Hahella sp. HN01 and exhibited excellent cellulase activity. The findings provide new insights into the cellulases produced by strain CR1 and their potential in lignocellulosic biomass pre-treatment.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Minjie Gao, Guoshuai Yang, Feifei Li, Zichao Wang, Xiuyu Hu, Yun Jiang, Jiajun Yan, Zhitao Li, Xiaobei Zhan
Summary: This study involved the production of curdlan oligosaccharides by expressing endo-11-1,3-glucanase in Pichia pastoris and constructing a co-culture system. The optimized conditions in a 7-L bioreactor led to a maximum yield of 18.77 g/L of curdlan oligosaccharides with 3-10 degrees of polymerization, presenting a novel strategy for oligosaccharide production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
C. Liu, X. Qin, B. Liu, X. Xu, A. Deng, Y. Zhang, Z. Zhang, W. Zhang
Summary: A pectin lyase gene pnlzj5b from Aspergillus niger ZJ5 was successfully identified and overexpressed in Pichia pastoris. The recombinant protein PNLZJ5B showed high activity towards citrus pectin and its expression efficiency was improved through codon optimization. PNLZJ5B exhibited optimal activity and stability at specific temperature and pH, and it also showed positive response to the degree of pectin methylation and had a higher juice yield compared to commercial pectin lyase. These favorable enzymatic properties make PNLZJ5B potentially useful in juice processing and other food-related applications.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jun Yang, Sunny Sharma, Peter Koetter, Karl-Dieter Entian
NUCLEIC ACIDS RESEARCH
(2015)
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Genetics & Heredity
Sunny Sharma, Jun Yang, Rob van Nues, Peter Watzinger, Peter Koetter, Denis L. J. Lafontaine, Sander Granneman, Karl-Dieter Entian
Article
Biotechnology & Applied Microbiology
Huiying Luo, Yaru Wang, Hui Wang, Jun Yang, Yuhui Yang, Huoqing Huang, Peilong Yang, Yingguo Bai, Pengjun Shi, Yunliu Fan, Bin Yao
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2009)
Article
Biotechnology & Applied Microbiology
Huiying Luo, Jun Yang, Peilong Yang, Jiang Li, Huoqing Huang, Pengjun Shi, Yingguo Bai, Yaru Wang, Yunliu Fan, Bin Yao
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2010)
Article
Biotechnology & Applied Microbiology
Huiying Luo, Yaru Wang, Jiang Li, Hui Wang, Jun Yang, Yuhui Yang, Huoqing Huang, Yunliu Fan, Bin Yao
ENZYME AND MICROBIAL TECHNOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Huiying Luo, Jiang Li, Jun Yang, Hui Wang, Yuhui Yang, Huoqing Huang, Pengjun Shi, Tiezheng Yuan, Yunliu Fan, Bin Yao
Article
Biochemistry & Molecular Biology
Sunny Sharma, Jun Yang, Peter Watzinger, Peter Koetter, Karl-Dieter Entian
NUCLEIC ACIDS RESEARCH
(2013)
Article
Biochemistry & Molecular Biology
Sunny Sharma, Jun Yang, Simon Duettmann, Peter Watzinger, Peter Koetter, Karl-Dieter Entian
NUCLEIC ACIDS RESEARCH
(2014)
Article
Multidisciplinary Sciences
Jun Yang, Sunny Sharma, Peter Watzinger, Johannes David Hartmann, Peter Koetter, Karl-Dieter Entian
Article
Biochemistry & Molecular Biology
Jun Yang, Huiying Luo, Jiang Li, Kun Wang, HaiPing Cheng, Yingguo Bai, Tiezheng Yuan, Yunliu Fan, Bin Yao
PROCESS BIOCHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Britta Meyer, Carina Immer, Steffen Kaiser, Sunny Sharma, Jun Yang, Peter Watzinger, Lena Weiss, Annika Kotter, Mark Helm, Hans-Michael Seitz, Peter Koetter, Stefanie Kellner, Karl-Dieter Entian, Jens Woehnert
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Sunny Sharma, Ewa Grudzien-Nogalska, Keith Hamilton, Xinfu Jiao, Jun Yang, Liang Tong, Megerditch Kiledjian
NUCLEIC ACIDS RESEARCH
(2020)
Article
Multidisciplinary Sciences
Xiaowen Wang, Hong Zhang, Russell Sapio, Jun Yang, Justin Wong, Xin Zhang, Jessie Y. Guo, Sharon Pine, Holly Van Remmen, Hong Li, Eileen White, Chen Liu, Megerditch Kiledjian, Dimitri G. Pestov, X. F. Steven Zheng
Summary: The study reveals a crucial role of nuclear SOD1 in ribosome biogenesis and proliferation in KRAS-driven lung cancer.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Sunny Sharma, Jun Yang, Ewa Grudzien-Nogalska, Jessica Shivas, Kelvin Y. Kwan, Megerditch Kiledjian
Summary: The cytoplasmic protein Xrn1 is found to be capable of degrading RNAs with an unprotected 5' monophosphate end as well as RNAs harboring noncanonical NAD caps. The deNADding activity of Xrn1 is crucial for normal growth and regulation of mitochondrial NAD-capped RNA levels and intramitochondrial NAD levels.
NATURE COMMUNICATIONS
(2022)