Article
Microbiology
Jun Song, Hongri Ruan, Li Chen, Yuqi Jin, Jiasan Zheng, Rui Wu, Dongbo Sun
Summary: SDQ, a specific lytic S. aureus bacteriophage, can effectively remove S. aureus biofilms. SDQ maintains its full activity under various environmental conditions and can be used in combination with detergents.
Article
Biochemistry & Molecular Biology
Sha Liu, Karen Hon, George Spyro Bouras, Alkis James Psaltis, Keith Shearwin, Peter-John Wormald, Sarah Vreugde
Summary: The isolated Staphylococcus aureus phages showed significant bactericidal activity against the biofilm form and a broad host range, with low mutation rate, indicating potential for further development as a treatment alternative to antibiotics for Methicillin-Resistant Staphylococcus Aureus (MRSA) infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
L. Zhang, K. Shahin, A. Soleimani-Delfan, H. Ding, H. Wang, L. Sun, R. Wang
Summary: Staphylococcus aureus biofilms are resistant to antibiotics, but the bacteriophage vB_SauS_JS02, isolated from livestock wastewater, showed activity against multidrug-resistant S. aureus. It has a broad host range, moderate pH stability, and appropriate thermal tolerance.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Fatemeh Mohammadian, Hamideh Kalateh Rahmani, Behnam Bidarian, Babak Khoramian
Summary: This study isolated two bacteriophages with specific lytic activity against Staphylococcus aureus strains from dairy farm sewage. The phages showed sensitivity to multidrug-resistant, methicillin-resistant, and biofilm-producing strains. Additionally, the phages maintained their lytic activity in milk.
BMC VETERINARY RESEARCH
(2022)
Article
Immunology
Elliot Whittard, James Redfern, Guoqing Xia, Andrew Millard, Roobinidevi Ragupathy, Sladjana Malic, Mark C. Enright
Summary: This study validates the potential of phage therapy for treating Staphylococcus aureus infections by examining the characteristics and host range of lytic S. aureus phages. Phages with the widest host range and effective killing abilities against various isolates were identified, showing promise as therapeutic candidates for future studies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Virology
Hyonoo Joo, Sijia M. Wu, Isha Soni, Caroline Wang-Crocker, Tyson Matern, James Peter Beck, Catherine Loc-Carrillo
Summary: Staphylococcus aureus is the main cause of implant-related infections in the form of biofilms. The heavy use of broad-spectrum antibiotics against these pathogens contributes to the problem of antibiotic-resistant infections. Bacteriophages have the potential to be an alternative therapy.
Article
Biotechnology & Applied Microbiology
Houqi Ning, Hong Lin, Jingxue Wang, Xuebing He, Xiaoqian Lv, Lei Ju
Summary: A phage qdsa002 targeting MRSA was isolated and its endolysin Lys84 and domains were purified. Lys84 showed strong lytic activity and effectively removed MRSA biofilms, with the combination of CHAP and Amidase_2 domains exhibiting close to 90% of Lys84's activities.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Georgios Katsipis, Anastasia A. Pantazaki
Summary: In this study, the antimicrobial effects of SPT on Staphylococcus aureus biofilms were demonstrated. SPT reduced the content of cell wall components, altered the activity of alkaline phosphatases and proteins involved in phosphate metabolism, and weakened the resistance of bacteria to biofilm formation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jumpei Fujiki, Tomohiro Nakamura, Keisuke Nakamura, Keita Nishida, Yurika Amano, Yusaku Watanabe, Satoshi Gondaira, Masaru Usui, Masaru Shimizu, Kazuhiko Miyanaga, Shinya Watanabe, Tomohito Iwasaki, Kotaro Kiga, Tomoko Hanawa, Hidetoshi Higuchi, Teiji Sawa, Yasunori Tanji, Yutaka Tamura, Longzhu Cui, Hidetomo Iwano
Summary: The study assessed the biological stability and clinical potential of Staphylococcus virus Phi SA012. The virus showed a wide host range and efficient lytic activity. Experiments on mice demonstrated the ability of Phi SA012 to spread and accumulate in specific organs without causing inflammation. Additionally, the study highlighted the importance of appropriate dosing and administration periods for effective phage therapy.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Farhana Parvin, Md. Arifur Rahman, Anand K. Deva, Karen Vickery, Honghua Hu
Summary: Staphylococcus aureus biofilms exhibit resistance to antibiotics and disinfectants, which is attributed to changes in the bacterial cell wall. Proteins involved in cell wall synthesis are upregulated in biofilms compared to planktonic organisms. Furthermore, the width of the cell wall and peptidoglycan production increase with biofilm culture duration and dehydration. Disinfectant tolerance is highest in dry surface biofilms, followed by hydrated biofilms, and lowest in planktonic bacteria, indicating that changes in the cell wall may contribute to the biocide resistance of S. aureus biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Dan Yang, Shuang Wang, Erchao Sun, Yibao Chen, Lin Hua, Xiangru Wang, Rui Zhou, Huanchun Chen, Zhong Peng, Bin Wu
Summary: This study isolated a temperate bacteriophage vB_Saus_PHB21 and found that its integration into the host MRSA increased the host's capacities of cell adhesion, anti-phagocytosis, and biofilm formation, leading to severe mortalities in infection.
Article
Food Science & Technology
Wanwan Hou, Shimo Kang, Jiang Chang, Xiaorong Tian, Chunlei Shi
Summary: The study demonstrated that by inducing GlpQ to degrade WTA, LBA effectively inhibits the biofilm formation of Staphylococcus aureus, reducing its adhesive ability.
Article
Biochemistry & Molecular Biology
Maria Kornienko, Dmitry Bespiatykh, Maja Malakhova, Roman Gorodnichev, Nikita Kuptsov, Egor Shitikov
Summary: Phage therapy is a hopeful approach to combat multidrug-resistant bacteria. However, it requires the isolation of new phages or the search for suitable phages in existing libraries. This study proposes a simple PCR method to differentiate between two families of Staphylococcus phages and eleven genera of Klebsiella phages, allowing for rapid screening and identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Vincent Jean-Pierre, Agathe Boudet, Pauline Sorlin, Quentin Menetrey, Raphael Chiron, Jean-Philippe Lavigne, Helene Marchandin
Summary: Staphylococcus aureus is a major human pathogen that can survive and cause diseases in various clinical settings, including Cystic Fibrosis. In Cystic Fibrosis, S. aureus is the most commonly identified opportunistic pathogen, and its colonization/infection can become chronic in about one third of patients. S. aureus's ability to form biofilm is a crucial factor contributing to its persistence and protection in the lungs of CF patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Elizabeth V. K. Ledger, Andrew M. Edwards
Summary: Most bactericidal drugs require bacterial replication and/or metabolic activity for their killing activity, but the lipopeptide antibiotic daptomycin has been reported to efficiently kill growth-arrested bacteria. However, a prolonged growth arrest can lead to daptomycin tolerance in Staphylococcus aureus, requiring glucose and increased production of certain cell wall polymers. This active cell wall remodelling sets daptomycin tolerance apart from other forms of antibiotic tolerance in nonreplicating bacteria.
