Article
Chemistry, Applied
Jinyu Yang, Xiwen Li, Shuangzhi Zhao, Wei Yuan, Qingxin Zhou, Yanhao Zhang, Jiying Qiu, Junhua Wang, Qingjun Zhu, Xiaoyu Yang, Xiaoxiao Jiang, Chengsen Tian, Leilei Chen
Summary: The effects of light calcium carbonate (CaCO3) on pullulan biosynthesis by Aureobasidium pullulans NCPS2016 were studied. Light CaCO3 significantly enhanced pullulan production, with an increase of 12.4% when added to a low concentration fructose broth compared to K2HPO4. The addition of light CaCO3 improved the activities of key enzymes and gene transcriptional levels related to pullulan biosynthesis, resulting in higher pullulan yields and productivity.
Article
Biochemistry & Molecular Biology
Fei Liu, Jinhua Zhang, Linjun Zhang, Mengqi Diao, Peixue Ling, Fengshan Wang
Summary: The study found a strong positive correlation between pullulan and melanin content; lack of melanin resulted in decreased pullulan yield, but could be increased by adding melanin; lack of pullulan could restore melanin synthesis, and high concentrations of pullulan induced morphological transformation and melanin synthesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Nan Zeng, Ning Zhang, Dandan Wang, Jiajia Long, Yunjiao Wang, Yating Zhang, Fangxiong Pu, Zijing Li, Faryal Babar Baloch, Bingxue Li
Summary: This study found that citric acid can regulate the cellular differentiation of Aureobasidium pullulans NG, thereby increasing pullulan production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Congyu Lin, Kai Zhang, Shanshan Zhao, Wan Wang, Xin Ru, Jinzhu Song, Hua Cong, Qian Yang
Summary: In this study, the strain Aureobasidium pullulans HIT-LCY3(T) was used to ferment potato starch waste to produce pullulan and melanin. The optimization of culture conditions led to high yields, and the use of adaptive evolution and air-floating fermenters further increased production. Small-scale and pilot tests confirmed the industrial potential of the process.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Chaoyong He, Zhen Zhang, Youdan Zhang, Guoliang Wang, Chonglong Wang, Dahui Wang, Gongyuan Wei
Summary: This study used cost-effective substrates to produce high molecular weight pullulan, optimized the medium using response surface methodology and artificial neural network, and analyzed the physiological mechanism of changes in pullulan titer and molecular weight using different substrates. Economic analysis showed a significant decrease in cost for using soybean meal hydrolysate and corn steep liquor substrates, improving the competitiveness of pullulan in industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Katia van den Eynde, Vik Boon, Rita Caiado Gaspar, Pedro Fardim
Summary: This study investigated the effects of sugar and mineral salt stresses on the pullulan production of A. pullulans ATCC 42023. Different pullulans with varying molecular weights were obtained by adjusting the conditions, and it was found that higher yield and lower polydispersity index were achieved when CuSO4 was added to the fermentation. Biofabrication of pullulan under stress conditions is a promising strategy to enhance biopolymer yield and obtain pullulan with a targeted molecular weight.
Article
Agricultural Engineering
Chaoyong He, Xuehan Zhang, Zhen Zhang, Chonglong Wang, Dahui Wang, Gongyuan Wei
Summary: In this study, corn starch, cob, and straw were biorefined and used as feedstocks for pullulan production. An evolved strain A. pullulans EV6 with strong adaptability and high capability of pullulan biosynthesis was screened. EV6 produced an increased titer of pullulan with a higher Mw than the parental strain PS. Whole-crop biorefinery of corn biomass confirmed its potential for efficient pullulan production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Materials Science, Characterization & Testing
Fabian Hernandez-Tenorio, Catalina Giraldo-Estrada
Summary: In this paper, the characteristics and chemical modification of pullulan produced by A. pullulans ATCC 15233 were studied. The pullulan derivative with a degree of substitution of 2.90 was synthesized through acetylation reaction, and its structure was elucidated using infrared and nuclear magnetic resonance spectroscopy. The results showed that pullulan exhibited typical glycosidic bonds and had a molecular weight of 93.47 kDa. Acetylation led to increased thermal stability and glass transition temperature. The pullulan film had homogeneous morphology and showed certain mechanical properties. The results suggest that biosynthesized pullulan and its derivatives have important physicochemical properties in the development of biomaterials.
Article
Environmental Studies
Ravichandran Viveka, Sunita Varjani, Nakkeeran Ekambaram
Summary: The study evaluated the use of cassava waste for pullulan production from Aureobasidium pullulans MTCC 1991 in solid-state fermentation, identifying optimal conditions for maximum yield. The analysis focused on the effects of yeast extract, K2HPO4, (NH4)(2)SO4, MgSO4 center dot 7H(2)O, and NaCl on pullulan production. Results confirmed that cassava waste can be an effective carbon source for pullulan production.
ENERGY & ENVIRONMENT
(2021)
Article
Food Science & Technology
Shoib Mohmad Wani, Sajad Ahmad Mir, F. A. Khanday, F. A. Masoodi
Summary: Pullulan is an important exopolysaccharide obtained from ubiquitous fungi Aureobasidium pullulans with wide applications in the food, pharmaceutical and biomedical industries. Agro-industrial waste has become a preferred substrate for pullulan production due to its economic and nutritional attributes. Various factors such as type of substrate, carbon source addition, nitrogen source, pH, aeration/agitation speed, and temperature influence pullulan production.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2021)
Article
Food Science & Technology
Yu-Ting Zhang, Yu-Ke Deng, Yong-Fang Zou, Bao-Lin Han, Ji-Zhou Pu, Jia-Quan Rao, Dan Huang, Hui-Bo Luo
Summary: This study investigated the changes in microbial functions and the relationship between carbohydrate metabolism-related functional genes and extracellular enzyme activity during Daqu fermentation. The results revealed that the pathways related to metabolites varied at different fermentation stages and that certain functional genes showed higher activity in the early and late stages. Amino sugar and nucleoside sugar metabolism dominated in the middle stage of fermentation. In addition, amylase activity positively correlated with carbon metabolism-related pathways, while liquefaction activity negatively correlated with these pathways.
Article
Biotechnology & Applied Microbiology
Shuyu Chen, Hongchen Zheng, Jiaqi Gao, Hui Song, Wenqin Bai
Summary: In this study, a novel yeast-like strain Aureobasidium pullulans BL06 was isolated, producing the highest molecular weight pullulan reported so far. Three gene knockout strains were constructed to further regulate the biosynthesis of pullulan, and one strain produced moderate molecular weight pullulan at the highest yield level to date, reducing production costs and expanding its application scope and potential.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Polymer Science
WesamEldin I. A. Saber, Abdulaziz A. Al-Askar, Khalid M. M. Ghoneem
Summary: Pullulan is a biodegradable, renewable, and environmentally friendly hydrogel biopolymer, which can be used in various fields. In this study, a new endophytic Aureobasidium pullulans strain was used for pullulan biosynthesis, and the fermentation process was optimized using Taguchi's approach and the decision tree learning algorithm. The results showed the accuracy and consistency of the important variables determined by both methods. The decision tree model further reduced the medium sucrose content without affecting pullulan biosynthesis. The optimized conditions yielded 7.23% pullulan.
Article
Biotechnology & Applied Microbiology
Christoph Keysberg, Helga Schneider, Kerstin Otte
Summary: This study presents a purification setup using tangential flow filtration (TFF) and fast-performance liquid chromatography (FPLC)-based size exclusion chromatography (SEC) suitable for industrial production of therapeutic extracellular vesicles (EVs). Potential host cell lines were evaluated, with telomerase-immortalized Wharton's jelly mesenchymal stromal cells (WJ-MSC/TERT273) found to secrete high amounts of regenerative EVs, and Cevec's amniocyte producer cells (R) (CAP (R)) and human embryonic kidney (HEK293) suspension cells suitable for designer EVs with high yields. Chemical adjuvants were used to enhance EV secretion in HEK293 cells, with the combination of fenoterol, iodoacetamide, and dinitrophenol increasing EV yield threefold and cellular secretion rate fivefold.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Kai Zhang, Congyu Lin, Shanshan Zhao, Wan Wang, Wei Zhou, Xin Ru, Hua Cong, Qian Yang
Summary: pH is an important factor affecting fungi. The pH transcription factor Appacc plays a vital role in the production of pullulan from potato waste.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Nanoscience & Nanotechnology
Yudong Cao, Bin Chen, Haibin Zhong, Liyuan Pei, Guanglei Liu, Zhenglong Xu, Jianfeng Shen, Mingxin Ye
Summary: This study reports the design of a flexible sensor using Ti2C3Tx/polyurethane composites prepared by gas-liquid interface self-assembly. The sensor has a wide detection range, low-stress detection limit, high sensitivity, and fast response time, making it suitable for various applications such as wearable motion monitoring, underwater human motion detection, and monitoring of fish motion in water.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Metallurgy & Metallurgical Engineering
Shun Guo, Hui-hui Zhang, Min-kyung Kwak, Wang Ding, Guang-lei Liu, Wen Ma, Hai-xia Liu, Qing-kun Meng, Xin-qing Zhao
Summary: The mechanisms responsible for deformation behavior in Nb/NiTi composite during pre-straining were systematically investigated. It was found that the composite experiences elastic elongation, slight plastic deformation, and stress-induced martensitic transformation during loading, while the deformation mechanisms during unloading mainly involve elastic recovery, compression deformation, and incomplete reverse SIM transformation.
TRANSACTIONS OF NONFERROUS METALS SOCIETY OF CHINA
(2022)
Article
Genetics & Heredity
Dengying Liu, Zhong Xu, Wei Zhao, Shiyi Wang, Tuowu Li, Kai Zhu, Guanglei Liu, Xiaoduo Zhao, Qishan Wang, Yuchun Pan, Peipei Ma
Summary: This study investigates the genetic parameters and genetic architectures of six milk production traits in the Shanghai Holstein population. The findings reveal different genetic mechanisms for milk production traits at different stages of lactation, which is significant for molecular breeding.
FRONTIERS IN GENETICS
(2022)
Article
Chemistry, Physical
Haibin Zhong, Guanglei Liu, Yudong Cao, Bin Chen, Mingxin Ye, Jianfeng Shen
Summary: Ni2P-Co2P nanowire arrays synthesized by hydrothermal and low-temperature phosphorization on nickel foam exhibit excellent catalytic performance with low overpotentials and high current densities in acidic, alkaline, and neutral solutions.
ACS APPLIED ENERGY MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Xin Wei, Miao Zhang, Guang-Yuan Wang, Guang-Lei Liu, Zhen-Ming Chi, Zhe Chi
Summary: In this study, engineered strains of A. pullulans var. aubasidani were found to be suitable for green fumaric acid production. The ornithine-urea cycle was identified as the key pathway for high level fumarate biosynthesis. Genetic modifications and overexpression led to the development of a strain that produced high yields of fumarate. This research is significant for global sustainable development and carbon neutrality.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xin Wei, Miao Zhang, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi
Summary: C-18:2 fatty acids play an important role in the cold and warm growth of psychrotrophic yeast, while C-18:3 fatty acids have no significant effect on cell growth. Additionally, the absence of C-18:2 fatty acids negatively affects cell wall development.
MARINE BIOTECHNOLOGY
(2023)
Article
Microbiology
Mei Zhang, Zhu Wang, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi
Summary: Reducing consumption of acetyl-CoA did not significantly enhance liamocin biosynthesis by engineered strains of A. melanogenm, but instead decreased cell growth. Careful selection of genes for expression is crucial to avoid negative impacts on liamocin production.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Materials Science, Multidisciplinary
Bin Chen, Guanglei Liu, Minying Wu, Yudong Cao, Haibin Zhong, Jianfeng Shen, Mingxin Ye
Summary: Currently, there is a growing interest in the development of organohydrogel-based wearable flexible electronics (WFEs) due to their superior environmental stability compared to hydrogel-based WFEs. In this study, an efficient strategy is proposed to achieve the quick preparation of organohydrogels by utilizing the high reactivity of eutectic gallium-indium alloy and constructing a glycerol/water binary solvent system. The resulting organohydrogels, named liquid metal-based ion-conducting organohydrogels (LMIOs), demonstrate excellent environmental adaptability, high transparency, and remarkable mechanical properties and self-healing abilities, making them suitable for sensing human motions and applications in skin-attachable devices, robotics, and prosthetics.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Physical
Guang-lei Liu, Wen-chao Xue, Yu-hao Cao, Xiao-xuan Sun, Zhi-qiang Li, Fu-hai Xu, Hai-xia Liu, Jian-zhong Zhou
Summary: This study investigates the effects of laser shock processing (LSP) on the thermal fatigue life and crack growth behavior of the coating and bonding area of the brake disc. The results show that LSP can enhance the thermal fatigue performance of the brake disc repaired by laser cladding.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Article
Materials Science, Multidisciplinary
Guanglei Liu, Wenchao Xue, Yuhao Cao, Zhiqiang Li, Xiaoxuan Sun, Fuhai Xu, Haixia Liu, Jianzhong Zhou
Summary: The effects of Laser Shock Peening (LSP) on the microhardness, residual stress, and microstructure of the Ni60 cladding layer and the combined area of the 20CrNiMo alloy were investigated. The results show that LSP can improve the microhardness, introduce residual compressive stress, and refine the microstructure. The residual compressive stress value is -538 MPa on the surface and -30 MPa at a depth of 2 mm.
JOURNAL OF LASER APPLICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Hao Chen, Cun-Cui Kong, Xin Wei, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi
Summary: In this study, an engineered strain of Aureobasidium melanogenum DFAK-ArgB6 overexpressing the ArgB gene was able to produce 3.0 g/L of L-piperazic acid from glucose during fed-batch fermentation. The yield was 55.6 mg L-piperazic acid/g glucose and the productivity was 24.8 mg/L/h, which is the highest reported so far. The partially purified L-piperazic acid had a purity of 86.4%.
PROCESS BIOCHEMISTRY
(2023)
Proceedings Paper
Computer Science, Artificial Intelligence
Liang Wang, Hui-nan Gu, Lei Zhao, Guang-lei Liu
Summary: This paper studies the motion characteristics and optimal U-turn position of left turn vehicles at signal-controlled intersections. It comprehensively analyzes the impact of left turn with U-turn on traffic flow from the aspects of driving efficiency and queue length.
2022 IEEE 25TH INTERNATIONAL CONFERENCE ON INTELLIGENT TRANSPORTATION SYSTEMS (ITSC)
(2022)
Article
Chemistry, Multidisciplinary
Wei Yao, Jian Tan, Xiaodong Zhu, Guanglei Liu, Zhenglong Xu, Mingxin Ye, Jianfeng Shen
Summary: Inspired by leaf structure, a carbon felt/PVA solar-driven device was developed for the separation of versatile oily wastewater. This device exhibits superior solar energy absorption and vapor release capacity, enabling efficient purification of different types of wastewater, including high concentration oil-water emulsions and acidic/alkaline wastewater.
CELL REPORTS PHYSICAL SCIENCE
(2022)
Article
Green & Sustainable Science & Technology
Yutong Feng, Guanglei Liu, Lei Li, Jie Ma, Chi Zhang, Jinhu Yang
Summary: Ni-doped FeS2 nanowire arrays (Ni-FeS2 NWs), fabricated through a simple doping strategy, exhibit enhanced antiacid stability and catalytic activity for the hydrogen evolution reaction (HER) compared to FeS2. This is attributed to the different HER active centers at S and Fe sites in Ni-FeS2 and FeS2, respectively.
ADVANCED ENERGY AND SUSTAINABILITY RESEARCH
(2022)
Article
Marine & Freshwater Biology
Guanshuo Yu, Xiaoxiang Wang, Chao Zhang, Zhe Chi, Zhenming Chi, Guanglei Liu
Summary: In this study, a mangrove yeast strain Moesziomyces aphidis XM01 was used for efficient extracellular production of mannosylerythritol lipids (MELs). The produced MELs showed excellent physicochemical properties and various biological functions. The MELs were further self-assembled into nanomicelles with good stability and antibacterial activity. This research provides insights into efficient MEL production and broad application prospects of MEL nanomicelles.
MARINE LIFE SCIENCE & TECHNOLOGY
(2022)
