Article
Plant Sciences
Rui-Fang Li, Pei-Dong Ren, Da-Pei Zhang, Ping Cui, Gui-Ning Zhu, Xiao-Yong Xian, Ji-Liang Tang, Guang-Tao Lu
Summary: This study investigates the role of HpaP homologues in the bacterial pathogens Xanthomonas oryzae pathovars oryzae (Xoo) and oryzicola (Xoc). The findings show that HpaP is required for the full virulence of Xoo and Xoc, and its deletion leads to a reduction in virulence and changes in the production of virulence factors. The study also reveals that HpaP plays a divergent role in Xoo and Xoc, indicating different infection strategies employed by these two pathogens.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biology
Anna Isabell Weaver, Laura Alvarez, Kelly M. Rosch, Asraa Ahmed, Garrett Sean Wang, Michael S. van Nieuwenhze, Felipe Cava, Tobias Dorr
Summary: The peptidoglycan cell wall is a crucial structure for bacteria, with the ability to determine cell shape and support survival. Peptidoglycan is a dynamic process that requires regulated synthesis and turnover. This study uncovers the physiological role of lytic transglycosylases (LTGs), a class of autolytic enzymes, and demonstrates their essentiality for bacterial survival. The study also suggests that LTGs prevent toxic accumulation of peptidoglycan polymers and their function is independent of peptidoglycan synthesis.
Article
Plant Sciences
Rajkanwar Nathawat, Roshan V. Maku, Hitendra K. Patel, Rajan Sankaranarayanan, Ramesh V. Sonti
Summary: This study uncovers a previously unknown function of the FnIII domain in promoting the virulence activity of the secreted virulence factor CbsA in Xanthomonas oryzae pv. oryzae.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Yanan Shao, Guiyu Tang, Yuanyuan Huang, Wenli Ke, Shasha Wang, Dehong Zheng, Lifang Ruan
Summary: The transcriptional regulator Sar plays a crucial role in the pathogenicity of Xoo against rice by regulating multiple secretion systems and related genes.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Virology
Muchen Zhang, Jiahui Qian, Xinyan Xu, Temoor Ahmed, Yong Yang, Chenqi Yan, Mohsen Mohamed Elsharkawy, Mohamed M. Hassan, Jamal A. Alorabi, Jianping Chen, Bin Li
Summary: This study reveals the significance of glycosyltransferase in interactions among pathogenic bacteria, phages, and plant hosts, shedding light on the balanced coevolution from an environmental perspective.
Article
Biochemistry & Molecular Biology
Aysha B. Mezoughi, Chiara M. Costanzo, Gregor M. Parker, Enas M. Behiry, Alan Scott, Andrew C. Wood, Sarah E. Adams, Richard B. Sessions, E. Joel Loveridge
Summary: Lytic transglycosylases like Slt35 are potential targets for novel antibacterial agents. Thionine acetate was found to be a weak inhibitor of both Slt35 and lysozyme, and also enhanced the efficacy of ampicillin against E. coli.
Article
Agriculture, Multidisciplinary
Dongni Gao, Hui Li, Jiang Shao, Lulu He, Chen Fu, Hongyu Lai, Dylan O'Neill Rothenberg, Xiaoli Xu, Gaopeng Song, Xin Deng, Zi-Ning Cui
Summary: Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a highly destructive bacterial disease. Traditional prevention methods using antibiotics have led to the emergence of resistant strains. New prevention techniques involving Type III secretion system (T3SS) inhibitors that target bacterial virulence factors without affecting bacterial growth have been developed. The study focused on the design and synthesis of ethyl-3-aryl-2-nitroacrylate derivatives for T3SS inhibition. Compounds B9 and B10 showed significant inhibition of hypersensitive response (HR) and T3SS gene expression in tobacco, and in vivo assays demonstrated their effectiveness in controlling BLB when combined with the quorum quenching bacteria F20.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Huihui Wang, Fan Chen, Guiyu Tang, Wenli Ke, Shasha Wang, Dehong Zheng, Lifang Ruan
Summary: In this study, we identified a transcriptional regulator called Gar (PXO_RS11965) that is involved in the regulation of bacterial growth and virulence in Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight in rice. Knockout of gar increased Xoo's virulence to rice, and RNA-sequencing and GUS assays showed that Gar positively regulates the expression of a σ54 factor called rpoN2. Further experiments confirmed that overexpression of rpoN2 restored the phenotypic changes caused by gar deletion. Our research revealed that Gar influences bacterial growth and virulence by positively regulating the expression of rpoN2.
CURRENT MICROBIOLOGY
(2023)
Article
Microbiology
Amina Nazir, Zhaoxia Dong, Jin Liu, Rana Adnan Tahir, Neelma Ashraf, Hong Qing, Donghai Peng, Yigang Tong
Summary: A novel phage Xoo-sp15 that infects X. oryzae was isolated and characterized in this study, showing potential as a candidate for phage therapy against bacterial infections. High throughput sequencing revealed its dsDNA genome with unique features and in silico analysis confirmed its ability to infect multiple X. oryzae strains without containing resistant determinants.
CURRENT MICROBIOLOGY
(2021)
Article
Plant Sciences
Julien S. Luneau, Aude Cerutti, Brice Roux, Sebastien Carrere, Marie-Francoise Jardinaud, Antoine Gaillac, Carine Gris, Emmanuelle Lauber, Richard Berthome, Matthieu Arlat, Alice Boulanger, Laurent D. Noel
Summary: Analysis of Xanthomonas campestris pv. campestris transcriptome 3 days after entering cauliflower hydathodes revealed differential expression of 18% genes, with a striking repression of chemotaxis and motility functions. Although most virulence factors were not activated, the expression of 95 genes including those coding for type III secretion machinery was induced. Metabolic adaptations and limited stress responses were observed, with high-affinity phosphate transport confirmed to be important for bacterial fitness inside hydathodes.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Seiji Tsuge, Yumi Ikawa
Summary: Sucrose exudation induced by TAL effectors is essential for the virulence of X. oryzae pv. oryzae. Xylose is crucial for the expression of hrp genes, and regulators of xylan/xylose utilization and galactose metabolism are involved in hrp gene regulation. This review discusses the relationship between hrp gene expression and sugars/sugar metabolism in X. oryzae pv. oryzae.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Hui Wang, Yan Bi, Yizhou Gao, Yuqing Yan, Xi Yuan, Xiaohui Xiong, Jiajing Wang, Jiayu Liang, Dayong Li, Fengming Song
Summary: ONAC096, an inducible NAC transcription factor, contributes positively to rice immunity against Magnaprothe oryzae and Xanthomonas oryzae pv. oryzae by directly binding to the promoters of downstream target genes, enhancing the immune response and resistance to these pathogens.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Anna Joe, Valley Stewart, Pamela C. Ronald
Summary: When a bacterial pathogen infects a susceptible plant host, it expresses specific virulence factors to invade. In the case of X. oryzae pv. oryzae, the bacterium relies on the HrpX protein to transcribe the raxX and raxST genes, controlling the production of the RaxX sulfopeptide, which activates immune response in Xa21(+) rice lines. This coordinated gene expression with pathogenesis-related genes is crucial for successful infection.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Plant Sciences
Lifang Zou, Cuiping Zhang, Yilang Li, Xiaofei Yang, Yanyan Wang, Yichao Yan, Ruihuan Yang, Mengsang Huang, Fazal Haq, Ching-Hong Yang, Gongyou Chen
Summary: This research developed a modular plasmid assembly system for transferring functional DNA modules, accelerating gene expression analysis studies of Xoo and Xoc.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Virology
Pham D. T. My, Tu Q. Vinh, To H. Ngoc, Pham N. Q. Anh, Le T. M. Duyen, Nguyen M. Thien, Le T. T. Tien, Vo T. Phuc, Huynh N. Oanh, Le P. Nga, Hoang A. Hoang
Summary: Bacteriophage L522 was isolated from a paddy leaf sample in Long An province, Vietnam, and infects Xanthomonas oryzae pv. oryzae. Transmission electron microscopy revealed its myovirus morphology. It has a latency period of approximately 3 hours and a burst size of 63 new virions per infected cell (PFU/infected cell). The genome of L522 is 44,497 bp long with a GC content of 52%. Out of the 63 identified genes, 26 were predicted to have functions, and no virulence or antibiotic-resistance genes were detected. A BLASTn search revealed similarity to a previously reported Xanthomonas phage, with an average nucleotide sequence identity of 85% and a query coverage of 87.15%. Thus, L522 represents a new species in the genus Xipdecavirus.
ARCHIVES OF VIROLOGY
(2023)