Review
Food Science & Technology
Ravichandra Hospet, Devarajan Thangadurai, Natalia Cruz-Martins, Jeyabalan Sangeetha, Konerira Aiyappa Anu Appaiah, Zaira Zaman Chowdhury, Namita Bedi, Kasem Soytong, Abdel Rahman Mohammad Al Tawahaj, Shoukat Jabeen, Misbah Mubeen Tallur
Summary: Strain improvement technology plays a crucial role in enhancing the quality of industrial strains. The genome shuffling technique, which involves inducing mutation and recursive protoplast fusion, ensures specific phenotype improvement and does not require gene sequence data.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biotechnology & Applied Microbiology
Qingchao Jin, Haipeng Liao, Yanping Dou, Na Shen, Zhige Wu, Yu Yang, Zhihua Jin
Summary: Study on the AfsKRS system in Streptomyces pristinaespiralis revealed that AfsR serves as a crucial regulator with differential effects on the biosynthesis of pristinamycin I and II components, while AfsK has a positive correlation with pristinamycin I biosynthesis and a negative correlation with pristinamycin II biosynthesis. This research provides valuable insights into the regulatory function of the AfsKRS system in pristinamycin production.
Article
Biotechnology & Applied Microbiology
Fatema M. Abd Allah, Asmaa M. Elhosiny, Hala F. Mohamed, Ayman A. Farrag, Magda A. Elmeleigy
Summary: In this study, lactic acid bacteria (LAB) isolated from milk were evaluated for their antibacterial properties. Genome shuffling was utilized to enhance the antimicrobial activity of these LAB isolates. Results showed that 31 strains out of the 61 isolates exhibited antibacterial activity against tested pathogens, with Lactobacillus plantarum CIP 103151 and Lactobacillus plantarum JCM 1149 demonstrating the highest antimicrobial activity. By applying the genome shuffling approach, the inhibitory zones against Staphylococcus aureus, Salmonella typhimurium, Pseudomonas aeruginosa, and Escherichia coli were significantly increased. Random Amplified Polymorphic DNA analysis revealed clear differences in DNA banding patterns between the wild strain of L. plantarum CIP 103151 and the selected shuffled strains.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Salah M. Aleid, Siddig H. Hamad, Stephane Delaunay, Michel Fick, Eric Olmos
Summary: By optimizing the substrates and culture conditions, the biosynthesis of Pristinamycin was successfully achieved, with date sirup as a substrate showing higher yield compared to glucose. The stirring rate plays a significant role in antibiotic formation.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yang Song, Zixuan Zhang, Xiangli Zhang, Jiayi Yao, Xiaoping Yu, Xuping Shentu
Summary: In this study, the production of toyocamycin in Streptomyces diastatochromogenes 1628 was enhanced through three rounds of genome shuffling screening. The optimization of nutrition condition in medium resulted in the highest production of toyocamycin. The mechanism for the improvement of shuffled strains was investigated, revealing the role of transcriptional level, product resistance, and ATP-binding pathway-specific regulator.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Nourah Hassan Alzahrani, Alaa Ahmed Mohamed El-Bondkly, Mervat Morsy Abbas Ahmed El-Gendy, Ahmed Mohamed El-Bondkly
Summary: Genetic manipulation and engineered culture medium were used to increase undecylprodigiosin production, while solid-state fermentation was applied to reduce production costs. The recombinant strain ALAA-R20 showed significantly higher yields than its parental strains ESRAA-10 and ESRAA-31, and exhibited strong antimicrobial and anti-cancer activities.
JOURNAL OF APPLIED GENETICS
(2021)
Article
Microbiology
Jiaqi Xie, Yinghao Yu, Junjiang You, Zhiwei Ye, Fenglong Zhou, Na Wang, Jingru Zhong, Liqiong Guo, Junfang Lin
Summary: This study successfully generated seven novel fusion strains of Ganoderma spp. with enhanced Ergothioneine (EGT) yields. The fusion strain RS7 showed a significant increase in mycelial EGT production, surpassing the parental strains FQ16 and FQ23 by 34.23% and 39.10%, respectively. Genomic analysis of the RS7 strain revealed mutations in the gene associated with EGT metabolism, leading to a redirection of substrate towards the EGT synthesis pathway and a boost in EGT production. These findings provide valuable guidance for commercial-scale production of EGT and selective breeding of Ganoderma spp. strains.
Article
Biotechnology & Applied Microbiology
Liting Ye, Ruifang Ye, Fengxian Hu, Guozhu Wang
Summary: FK506, an immunosuppressive polyketide produced by Streptomyces tsukubaensis, showed significantly increased production levels through atmospheric and room temperature plasma mutagenesis and genome shuffling techniques. The addition of exogenous DMSO further enhanced FK506 production. The newly isolated strain R3-C4 exhibited altered morphology and gene expression profiles compared to the wild-type strain, potentially leading to improved FK506 biosynthesis.
BIOTECHNOLOGY LETTERS
(2021)
Article
Microbiology
Yongjuan Liu, Kaifang Wang, Long Pan, Xusheng Chen
Summary: In this study, a better ε-PL producing strain was generated through genome shuffling on strains obtained by ribosome engineering. The mutant strain SG-86 produced 144.7% more ε-PL than the parent strain M-Z18. Illumina sequencing was used to map the genomic changes leading to the improved phenotype, revealing a smaller genome size, metabolic pathway changes, and gene deletions in the mutant strain. This study is of great significance for improving fermentation performance and providing theoretical guidance for metabolic engineering of ε-PL producing strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Manee Chanama, Chanwit Suriyachadkun, Suchart Chanama
Summary: In this study, a novel actinobacterium called strain SMC 277(T) was isolated from clay soil in a paddy field in Thailand. The strain was characterized using various taxonomic methods and was identified as a member of the genus Streptomyces. Genome sequencing revealed the presence of 35 biosynthetic gene clusters encoding diverse bioactive secondary metabolites, and the strain showed antimicrobial activity against drug-resistant bacteria associated with nosocomial infections. Based on these findings, the strain was proposed as a new species called Streptomyces antimicrobicus.
Article
Microbiology
Chen Xue, Jiaxin Zheng, Guangli Wang, Liang Feng, Feng Li
Summary: A functional strain BD2 with improved degradation efficiency of chlorothalonil (CTN) and carbendazim (MBC) was obtained through protoplast fusion, and the genetic relationship between BD2 and its parental strains was confirmed by SEM, RAPD, and 16S rRNA gene sequences analysis. BD2 showed better degradation rates of CTN and MBC compared to its parental strains, indicating its potential for bioremediation of CTN and MBC contaminated sites.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Alaa Ahmed Mohamed El-Bondkly, Mervat Morsy Abbas Ahmed El-Gendy, Ahmed M. A. El-Bondkly
Summary: The study isolated a marine endophytic fungus,Fusarium sp. ALAA-20, from a marine spongeAplysina fistularis, with potential for high lovastatin production. Through optimization methods, a hyperactive recombinant strain FR3/1 was obtained, showing significantly increased lovastatin production and promising antitumor and antibacterial properties.
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING
(2021)
Article
Plant Sciences
Simone Scintilla, Umberto Salvagnin, Lisa Giacomelli, Tieme Zeilmaker, Mickael A. Malnoy, Jeroen Rouppe van der Voort, Claudio Moser
Summary: This study has developed a successful single-cell based, DNA-free method to edit grapevine varieties while maintaining the original genetic makeup, providing a powerful tool for the application of NBTs in grape breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Rui Wang, Xinzhu Li, Shuifang Zhu, Demei Zhang, Shijian Han, Zhigang Li, Jiahui Lu, Haiwei Chu, Jiming Xiao, Suli Li
Summary: This study investigated the fusion of sugarcane protoplasts using various methods and found that fusion led to decreased viability, damaged cell membranes, and altered expression of key regeneration genes. These findings provide a theoretical basis for understanding the mechanisms underlying protoplast fusion.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Keke Xia, Dengwei Zhang, Xiaojing Xu, Guangyu Liu, Yong Yang, Zhiyong Chen, Xiaojuan Wang, Guo-Qiang Zhang, Hai-Xi Sun, Ying Gu
Summary: This study established a fast and convenient Phalaenopsis protoplast technology for the identification of functional genome editing tools. Two multiplex genome editing systems, PTG-Cas9-HPG and RMC-Cpf1-HPG, were developed and evaluated in Phalaenopsis. The results showed that both systems are functional in Phalaenopsis, and the Super promoter can significantly improve the editing efficiency of the RMC-Cpf1-HPG system. This research provides a valuable foundation for developing diverse and efficient genome editing toolkits and facilitating precise breeding of orchids.