期刊
APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH
卷 16, 期 3, 页码 3489-3500出版社
CORVINUS UNIV BUDAPEST
DOI: 10.15666/aeer/1603_34893500
关键词
Lactobacillus salivarius; metabolomics; hyper-osmotic stress; quantitative H-1 NMR spectroscopy; principal component analysis
资金
- National Project for Agro-product Quality & Safety Risk Assessment [GJFP 2018004, GJFP 2018011]
- Hainan Provincial Natural Science Foundation of China [318QN260]
- Belt and Road Initiative Project for Joint Investigation and Exploitation Evaluation of Agricultural Resources in Tropical Countries [ZYLH2018010412]
In their natural habitats and application environments, lactic acid bacteria are often exposed to osmotic changes. At metabolic level, bacteria are able to de novo synthesize or uptake specific solutes from environment to maintain constant turgor pressure. In the work, the H-1 nuclear magnetic resonance (NMR) spectroscopy based on intracellular metabolites profiles of Lactobacillus salivarius under hyper-osmotic growth conditions was characterized. New characteristic peaks of compatible compounds, such as betaine, carnitine, choline and/or their derivatives were detected. Especially, the cytoplasmic concentrations of proline increased dramatically when the osmolarity was raised from about 0.40 to 1.84 osmol/kg by adding 0.8 mol/l NaCl to the medium. Comparative analysis of the metabolites of L. salivarius FDB89 grown under aerobic and anaerobic conditions with NaCl stress revealed an overlap of up-regulated compounds of more than 50%. 2-aminobutyrate, pyruvate, glycine, trimethylamine-N-oxide, and acetylcholine were detected only under anaerobic condition, and 2-oxoglutarate, tyrosine, glutamine and glutamate were detected only under aerobic condition, which suggested oxygen also has a nonnegligible effect on osmotic response in L. salivarius FDB89.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据