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Growth of Methylosinus trichosporium OB3b on methane and poly-β-hydroxybutyrate biosynthesis

APPLIED BIOCHEMISTRY AND MICROBIOLOGY (2008)

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APPLIED BIOCHEMISTRY AND MICROBIOLOGY

卷 44, 期 2, 页码 182-185

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PLEIADES PUBLISHING INC

DOI: 10.1134/S0003683808020099

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Biotechnology & Applied Microbiology Microbiology

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Optimal conditions for batch cultivation of the obligate methanotroph Methylosinus trichosporium OB3b on methane without superatmospheric pressure were chosen. The yield of absolutely dry biomass after 120 h of growth reached 20 g/l. This biomass contained 30% poly-beta-hydroxybutyrate (PHB) with molecular weight 300 kDa. The growth process included the stages of biomass growth and PHB biosynthesis. The latter stage occurred under nitrogen-deficiency conditions. It was accompanied by an increase in the activity of PHB biosynthesis enzymes (beta-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase) and the main NAD(P)H producer, methylenetetrahydromethanopterin dehydrogenase. The activity of PHB depolymerase increased insignificantly.

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