Article
Chemistry, Physical
Helena D. D. Tjornelund, Jesper Brask, John M. M. Woodley, Gunther H. J. Peters
Summary: In this study, an optimized protocol for controlling the initial water activity in organic reaction mixtures with soluble lipase was proposed. The protocol ensures reproducible and consistent enzyme activity measurements. The importance of consistent stirring and volume correction to compensate for solvent evaporation was highlighted. The study also tested the protocol on soluble and immobilized lipase and found similar water activity profiles and the highest enzyme activity in hexane. This optimized protocol expands the possibilities for studying enzyme kinetics.
Article
Biochemistry & Molecular Biology
Hae-Rin Jeong, Ju-Soon Yoo, Yong-Lark Choi, Yu-Sin Jang, Yong-Suk Lee
Summary: Alginate and its derivatives are natural polymers that are annually produced in large quantities and applied to various industries. MtAl138, a newly discovered alginate lyase, shows outstanding enzymatic activity at concentrated salt solutions and organic solvents, making it a reasonably attractive enzyme for industrial use.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Polymer Science
Hong Wang, Hongpeng Li, Chee Keong Lee, Noreen Suliani Mat Nanyan, Guan Seng Tay
Summary: This article presents the reaction modes of enzyme-catalyzed polyester synthesis and the mechanism of lipase-catalyzed polymerization. It also discusses the advantages and disadvantages of enzyme-catalyzed polyester synthesis under different solvent systems. Furthermore, it examines the challenges for further industrialization of this method.
Article
Biotechnology & Applied Microbiology
Alice Vetrano, Isabella Daidone, Nicoletta Spreti, Matteo Capone
Summary: The effect of adding water-soluble organic solvents (t-butanol and acetonitrile) on the catalytic activity of lipase B was studied. It was found that t-butanol can enhance the enzyme activity, while acetonitrile improves the enzyme-substrate affinity. Molecular dynamic simulations provided insights into the mechanisms underlying these effects.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Amira Hassan Alabdalall, Norah A. Al-Anazi, Lena A. Aldakheel, Fatma H. I. Amer, Fatimah A. Aldakheel, Ibtisam M. Ababutain, Azzah I. Alghamdi, Eida M. Al-Khaldi
Summary: This research conducted a biochemical characterization of lipase activity and stability for two strains of Aspergillus niger, identifying optimal conditions for enzyme activity under different temperatures and pH levels. Additionally, the study explored the effects of organic solvents, surfactants, and metal ions on lipase activity, providing insights for potential industrial applications. The results demonstrated that both strains exhibited robust lipase activity in detergent formulations, with promising potential for practical use in industrial settings.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Hoang Thi Hong Anh, Esmaeil Shahsavari, Nathan J. Bott, Andrew S. Ball
Summary: This study investigated the co-culture of two halophilic bacteria, Marinirhabdus sp. and Marinobacter hydrocarbonoclasticus, and found that the co-culture resulted in increased protease activity and biomass production. Importantly, the protease activity in the co-culture remained active over a broader range of environmental conditions, making it an economical and practical approach for enhanced biotechnological applications.
Article
Microbiology
Senaite Leykun, Eva Johansson, Ramesh Raju Vetukuri, Elaine Berger Ceresino, Amare Gessesse
Summary: In this study, a thermostable lipase that is tolerant to organic solvents was obtained from a hot spring in the East African Rift Valley. The enzyme showed high catalytic activity in non-aqueous media and was stable in the presence of polar organic solvents. This enzyme has potential for the synthesis of valuable compounds.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Abdallah R. Ismail, Hamdy Kashtoh, Kwang-Hyun Baek
Summary: The development of new biocatalytic systems, particularly temperature-resistant and solvent-tolerant lipases, is crucial for industrial applications in biodiesel, detergent, food, pharmaceutical, and other industries. The enhancement of lipase temperature-resistance and solvent-tolerance through biotechnological and biochemical techniques is essential for industrial use.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Physical
VinayKumar Dachuri, Sei-Heon Jang, ChangWoo Lee
Summary: Cold-adapted organic solvent-tolerant (OST) enzymes show increased flexibility in polar organic solvents. Mutations of specific ion pairs near the active site of Proteus mirabilis lipase (PML) affect its conformational flexibility and stability in thermal and organic solvent environments.
Article
Chemistry, Multidisciplinary
Ye Zhou, Jannik Nedergaard Pedersen, Jacob Nedergaard Pedersen, Nykola C. Jones, Soren Vronning Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Perriman, Renjun Gao, Zheng Guo
Summary: This study focuses on the anionization of a carboxylate-enriched mutant of Bacillus subtilis lipase A (8M) to produce biofluids with enhanced hydrolytic activity. The resulting biofluids show a 2.5-fold increase in activity compared to those based on anionic polymer surfactants. Additionally, the use of anion-type biofluids with Myoglobin (Mb) is evaluated, revealing partial recovery of the active alpha-helix level in the biofluids. These highly active solvent-free liquid enzymes exhibit increased thermal stability and open up new possibilities in solvent-free liquid protein research.
Article
Biotechnology & Applied Microbiology
Priyanka V. Jawale, Bhalchandra M. Bhanage
Summary: The study focused on analyzing reaction kinetics and mechanism for synthesizing propyl benzoate in solvent-free conditions, immobilizing lipase on HPMC and PVA polymer blend, with CCL showing excellent activity. Optimizing reaction conditions and calculating kinetic parameters revealed that propyl benzoate synthesis using immobilized CCL followed the ternary complex model.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
F. Rafiee, M. Rezaee
Summary: Immobilized enzymes, particularly lipase on chitosan support, have attracted significant attention due to their advantages in industry and biochemistry. This review highlights the recent studies on lipase immobilization and discusses the potential solutions for current limitations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Georgiana Necula-Petrareanu, Paris Lavin, Victoria Ioana Paun, Giulia Roxana Gheorghita, Alina Vasilescu, Cristina Purcarea
Summary: This study cloned and characterized a novel recombinant aldehyde dehydrogenase (F-ALDH) from the psychrotrophic Flavobacterium PL002 isolated from Antarctic seawater. The F-ALDH showed high catalytic efficiency, thermal stability, and broad substrate specificity, making it a promising catalyst for biotechnological applications.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Priyanka Priyanka, Gemma K. Kinsella, Gary T. Henehan, Barry J. Ryan
Summary: This study identified a strain of Listeria monocytogenes that secretes solvent tolerant lipase activity. The organism showed resistance to solvents commonly used in biotechnological applications. However, practical applications would require the use of non-pathogenic or attenuated strains of Listeria.
BIOTECHNOLOGY LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Yuan Ji, Zhengzhang Wu, Peng Zhang, Meng Qiao, Yi Hu, Baoxing Shen, Bingzhi Li, Xing Zhang
Summary: The study successfully encapsulated lipase and magnetic nanoparticles into ZIF-8 composite material using a mild and quick one-pot strategy, achieving high immobilization yield. The resulting magnetic MOF composite demonstrated excellent resistance to harsh environments, simplifying complex downstream operations, and maintaining high activity even after multiple reuse cycles.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Food Science & Technology
Mustika Sari, Yustine Chung, Felicia Agatha, Hyung Kwoun Kim
APPLIED BIOLOGICAL CHEMISTRY
(2019)
Article
Biotechnology & Applied Microbiology
Chae Gyeong Park, Jin Ju Kim, Hyung Kwoun Kim
ENZYME AND MICROBIAL TECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Se Hyeon Park, Hyung Kwoun Kim
ENZYME AND MICROBIAL TECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Sehyeon Park, Hyung Kwoun Kim
Summary: Ergo and cholesterol can enhance the performance of liposomes by increasing membrane packing density and physical stability but can reduce skin permeability. In this study, we synthesized Ergo-Est, which showed increased membrane stability without significantly reducing fluidity. Additionally, liposomes containing EL18:2 and ELn18:3 maintained membrane flexibility and skin permeability, making them potential sterol compounds for flexible liposome formulations.
CHEMISTRY AND PHYSICS OF LIPIDS
(2023)