Article
Biochemistry & Molecular Biology
Yuming Sun, Ting Zhang, Xiaoyang Xu, Yongheng Yang, Haiying Tong, Luis Alejandro Jose Mur, Haiyan Yuan
Summary: Nitrate nutrition was found to enhance the synthesis of steviol glycosides (SGs) in stevia leaves compared to ammonium nutrition at the same nitrogen level. Transcriptomic analysis identified 397 differentially expressed genes between nitrate and ammonium treatments. Further research suggested that nitrate can promote leaf SG synthesis through a specific NO3--MYB/WRKY-GGPPS/CPS module.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Carla Benelli, Lara S. O. Carvalho, Soumaya El Merzougui, Raffaella Petruccelli
Summary: This study successfully achieved long-term preservation of Stevia tissues using DV and V cryo-plate methods, with significant impacts of immersion time and treatment conditions on regrowth, and apical shoot tips being more suitable material for cryopreservation.
Article
Plant Sciences
Shiv Shanker Ashrita, Shiv Shanker Pandey, Ashish R. Warghat
Summary: This study reveals that PEG-treated plants at 1.5 mg L-1 perform better in terms of growth and yield attributes, as well as metabolite accumulation parameters, after 1 month of ex-vitro acclimatization.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Agronomy
Luis Alfonso Rodriguez-Paez, Ana Melisa Jimenez-Ramirez, Marcelo F. Pompelli, Yirlis Yadeth Pineda-Rodriguez, Alfredo Jarma-Orozco, Juan de Dios Jaraba-Navas, Hermes Aramendiz-Tatis, Enrique Combatt-Caballero, Maria Ileana Oloriz-Ortega, Novisel Veitia Rodriguez
Summary: The Germplasm Bank of Stevia rebaudiana segregants of the University of Cordoba is a strategy for the use of genetic variability and efficient crop yield. There are genotypes with important characteristics such as high tolerance to salt stress and climatic change, late flowering, erect architecture, and high contents of steviol glycosides. However, there is a lack of in-depth studies on morphophysiological and biochemical indicators, which could be used to develop early selection tools for elite genotypes.
Article
Plant Sciences
Reema Devi, Ahmed Abdulhaq, Rachna Verma, Kiran Sharma, Dinesh Kumar, Ajay Kumar, Ashwani Tapwal, Rahul Yadav, Syam Mohan
Summary: This study focused on enhancing the therapeutic potential of medicinal plants through inoculation with endophytic fungi. A total of twenty fungal strains were isolated from the medicinal plant Ocimum tenuiflorum, and the R2 strain exhibited the strongest antagonistic activity against plant pathogenic fungi. The findings suggest that endophytic fungus inoculation can significantly influence the biological properties of medicinal plants.
Article
Agronomy
Clarissa Clemente, Luciana G. Angelini, Roberta Ascrizzi, Silvia Tavarini
Summary: The study found that in the Mediterranean climate, most stevia plants in the second year were in full vegetative growth, resulting in higher yields and antioxidant activities; while in the first year, plants were at the beginning of the reproductive phase, with the highest SVglys leaf content. Different genotypes exhibited significant variations in morpho-productive and phytochemical characteristics.
Article
Engineering, Environmental
Yingying Lu, Shuijiao Liao, Yiran Ding, Ying He, Ziyi Gao, Danna Song, Wei Tian, Xing Zhang
Summary: The study found that treatment with Stevia rebaudiana Bertoni residue significantly increased the soil arsenic concentration and enhanced the ability of Pteris vittata L. to extract arsenic, providing a new economic and environmentally friendly method for remediation of arsenic-contaminated soil.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Agricultural Engineering
Hasan Sardar, Ammar Nisar, Muhammad Akbar Anjum, Safina Naz, Shaghef Ejaz, Sajid Ali, Muhammad Sameem Javed, Riaz Ahmad
Summary: Moringa leaf extract shows potential as a plant growth enhancer, with foliar application leading to significant increases in growth attributes, mineral content, and nutraceutical-related characteristics in Stevia rebaudiana. The 20% concentration of MLE resulted in the highest production and beneficial effects on the plant.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Yuming Sun, Xiaoyang Xu, Ting Zhang, Yongheng Yang, Haiying Tong, Haiyan Yuan
Summary: Transcriptome analysis revealed that nitrogen deficiency inhibited plant growth and leaf photosynthesis, but increased steviol glycosides content in stevia. The study also found that nitrogen deficiency may promote steviol glycosides synthesis by changing carbon metabolism flux or inducing specific transcription factors. The results provide deeper insight into the relationship between nitrogen nutrition and steviol glycosides synthesis in stevia plants.
PLANT CELL REPORTS
(2021)
Article
Agriculture, Multidisciplinary
Sun Yu-ming, Huang Xiao-lei, Zhang Ting, Yang Yong-heng, Cheng Xiao-fang, Xu Xiao-yang, Yuan Hai-yan
Summary: The study found that potassium deficiency significantly reduces steviol glycosides content in stevia leaves, inhibits the expression of genes related to steviol glycosides synthesis, and affects the activity of sugar metabolism-related enzymes. There is a significant positive correlation between sucrose, glucose, and steviol glycosides content.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Food Science & Technology
Dj'essica Tatiane Raspe, Camila da Silva, Silvio Claudio da Costa
Summary: This review analyzes and discusses emerging techniques for extracting natural sweeteners and active compounds from Stevia rebaudiana Bertoni, demonstrating their impacts on extraction and comparing them with conventional techniques. These new technologies offer the advantages of simplicity, reduced energy and solvent usage, making them potentially suitable for industrial applications, but they still face limitations.
Review
Agronomy
Shilpa Sharma, Swati Gupta, Deepa Kumari, Shanker Lal Kothari, Rohit Jain, Sumita Kachhwaha
Summary: Stevia rebaudiana, commonly known as sweet leaf, is a plant with medicinal and industrial importance due to its high content of zero-calorie natural sweeteners called steviol glycosides. However, the plant faces challenges in terms of poor seed germination and slow vegetative propagation, making it difficult to meet the global demand for Stevia-based products. Biotechnological approaches including in vitro organogenesis, callogenesis, and cell suspension cultures, as well as nutrient manipulation, elicitor use, hairy root cultures, genetic transformation, and metabolic pathway engineering have been developed to address these limitations and improve steviol glycoside production. This review provides an up-to-date report on the applications of various biotechnological tools for mass propagation and enhanced steviol glycoside production, along with a detailed bibliometric analysis, and highlights research gaps and future considerations for further exploration.
Article
Horticulture
Monika Tuleja, Michal Santocki, Michal Dziurka, Krystyna Musial, Ewa Capecka, Marta Libik-Konieczny
Summary: Seed germination in Stevia rebaudiana is poor, limiting generative reproduction. The study suggests that improper levels of gibberellins and redirection of metabolic pathways for secondary metabolites biosynthesis may cause difficulties in pollen tube germination, affecting seed development in the plant.
SCIENTIA HORTICULTURAE
(2021)
Article
Agricultural Engineering
Marcos Vinicius Silva de Andrade, Renato Delmondez de Castro, Diego da Silva Cunha, Valdir Gomes Neto, Maria Gabriela Aparecida Carosio, Antonio Gilberto Ferreira, Lourdes Cardoso de Souza-Neta, Luzimar Gonzaga Fernandez, Paulo Roberto Ribeiro
Summary: Research demonstrates that Stevia rebaudiana's response to light conditions affects the accumulation of antioxidant-like metabolites in its leaves, possibly linked to total phenolic content. Variations in SOD activity among plants under different photoperiods suggest a synergistic interaction between SOD isoforms and phenolic compounds to mitigate oxidative stress caused by light exposure.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Clara R. Azzam, Sudad K. Al-Taweel, Ranya M. Abdel-Aziz, Karim M. Rabea, Alaa I. B. Abou-Sreea, Mostafa M. Rady, Esmat F. Ali
Summary: The study aimed to propagate Stevia rebaudiana in vitro and investigated the salt stress tolerance of two varieties, revealing that Sugar High A3 is more tolerant than Spanti. Biochemical markers associated with salt tolerance, such as POD, PPO, and MDH, were identified, suggesting potential applications in stevia breeding programs.