Article
Plant Sciences
Olga A. Aleynova, Andrey R. Suprun, Nikolay N. Nityagovsky, Alexandra S. Dubrovina, Konstantin V. Kiselev
Summary: This study demonstrates that plant endophytes play a significant role in stimulating stilbene production in grape cell suspension, with endophytic fungi showing a more pronounced induction effect on stilbene accumulation compared to endophytic bacteria. The increase in stilbene content was due to the activation of phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) gene expression. The findings suggest that grape endophytes could potentially serve as stimulators for stilbene biosynthesis in grapevine or grape cell cultures.
Article
Plant Sciences
Alexey A. Ananev, Andrey R. Suprun, Olga A. Aleynova, Nikolay N. Nityagovsky, Zlata Ogneva, Alexandra S. Dubrovina, Konstantin Kiselev
Summary: Stilbenes, known as plant defense compounds, rapidly accumulate in response to microbial infection and abiotic stresses. VaMyb40 and VaMyb60 genes, when overexpressed in Arabidopsis cell cultures, significantly increase stilbene content.
Article
Horticulture
Shu-Zhen Jiao, Chao Guo, Wen-Kong Yao, Ning-Bo Zhang, Ji-Yuan Zhang, Wei-Rong Xu
Summary: VaHsfC1, a heat stress transcription factor from Vitis amurensis, responds to heat, cold, ABA, and salinity stress, and its overexpression in Arabidopsis enhances heat and cold tolerance. It may play a crucial role in mediating multiple abiotic stress responses in plants.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Olga A. Aleynova, Nikolay N. Nityagovsky, Alexandra S. Dubrovina, Konstantin Kiselev
Summary: This study analyzed the composition of bacterial endophytes in wild Amur grape in the south of the Russian Far East using two different methods. The results showed that the dominant endophytes in V. amurensis were Gammaproteobacteria, Alphaproteobacteria, Actinobacteria, and Bacilli. NGS revealed a greater diversity of classes and genera compared to the cultivation-dependent method. The study also found that the composition of endophytic bacteria varied depending on the plant organ, grape individuals, environmental conditions, and sampling time.
Article
Plant Sciences
Andras Csoto, Antal Nagy, Nora Laurinyecz, Zora Annamaria Nagy, Csaba Nemeth, Erzsebet Krisztina Nemeth, Anna Csikasz-Krizsics, Nandor Rakonczas, Florence Fontaine, Erzsebet Fekete, Michel Flipphi, Levente Karaffa, Erzsebet Sandor
Summary: Grapevine trunk diseases (GTDs) pose an increasing threat to grape production worldwide. Different grape cultivars show varying degrees of susceptibility to GTDs. A study on Hungarian grape germplasm collections found that Vitis vinifera L. cultivars were more sensitive to GTDs compared to interspecific hybrids containing other Vitis species in their pedigree. The ancestral diversity of grapes confers greater resilience against GTDs.
Article
Plant Sciences
Olga A. Aleynova, Andrey R. Suprun, Alexey A. Ananev, Nikolay N. Nityagovsky, Zlata V. Ogneva, Alexandra S. Dubrovina, Konstantin V. Kiselev
Summary: This study investigated the effect of overexpression of VaCML genes on stilbene and biomass accumulation in Vitis amurensis cell cultures. The results showed that overexpression of VaCML65 gene significantly increased stilbene content, while overexpression of other VaCML genes had inconsistent effects. The VaCML65 gene is suggested to be a strong positive regulator in the stilbene biosynthesis pathway, which could be useful for obtaining grape cultivars with high stilbene content and stress resistance in viticulture and winemaking.
Article
Agriculture, Multidisciplinary
Ru Wang, Sangram Keshari Lenka, Varun Kumar, Kelem Gashu, Noga Sikron-Persi, Irena Dynkin, David Weiss, Avichai Perl, Aaron Fait, Michal Oren-Shamir
Summary: The study focuses on enhancing the production of stilbenes, specifically the resveratrol dehydrodimer viniferin, by overexpressing stilbene synthase (STS) and providing ample phenylalanine (Phe). Results show that this strategy significantly increases the accumulation of resveratrol and viniferin in plant cell cultures.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Genetics & Heredity
Yan Men, Ji-Rui Li, Hai-Lin Shen, Yi-Ming Yang, Shu-Tian Fan, Kun Li, Yin-Shan Guo, Hong Lin, Zhen-Dong Liu, Xiu-Wu Guo
Summary: A candidate gene VaAPRT3 related to sex determination was identified in V. amurensis, which could distinguish female individuals based on a 22-bp InDel marker. The gene was found to be involved in sex determination in V. amurensis by regulating cytokinin metabolism, providing a useful tool for sexual identification and germplasm collection.
FRONTIERS IN GENETICS
(2021)
Article
Chemistry, Organic
Ying Chen, Yiyao Fan, Yanqiu Li, Chunsuo Yao
Summary: A facile and versatile protocol for the efficient synthesis of 3-aryl-2,3-dihydrobenzofuran (ADB) has been reported. This reaction involves the cyclization and aryl migration reaction of ortho-hydroxystilbene in ethanol mediated by an iodonium ion, under ambient conditions. A class of ADB was efficiently prepared in good to excellent yields. Mechanistic investigation revealed that acids and alcohols facilitated aryl migration, while alkaline and non-alcohol solvents promoted β elimination. The practicality of this strategy was further demonstrated through two scale-up reactions and efficient synthetic elaboration.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Galina N. Veremeichik, Dmitry V. Bulgakov, Yuliya A. Konnova, Evgenia V. Brodovskaya, Valeria P. Grigorchuk, Victor P. Bulgakov
Summary: The overexpression of AtCPK1-Ca form increases pathogenesis-related protein levels in Vitis amurensis cell cultures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hajer Ben Ghozlen, Sven Mangelinckx, Stefaan P. O. Werbrouck
Summary: Stilbenes, including resveratrol, are bioactive compounds in peanuts. This study investigates the production of these compounds from Arachis hypogaea using in vitro systems, specifically callus cultures and cell suspension cultures. Biotic elicitors from various fungi were found to enhance the production of stilbenes, indicating a potential method for production and a new opportunity for obtaining resveratrol and its derivatives.
PROCESS BIOCHEMISTRY
(2023)
Article
Plant Sciences
Yi Wang, Haiping Xin, Peige Fan, Jisen Zhang, Yongbo Liu, Yang Dong, Zemin Wang, Yingzhen Yang, Qing Zhang, Ray Ming, Gan-Yuan Zhong, Shaohua Li, Zhenchang Liang
Summary: Vitis amurensis (Shanputao), the most cold-tolerant species of grape, has a genome assembly revealing a common ancestor with V. vinifera and potential gene expansions contributing to cold tolerance. Transcriptome analysis identified 17 genes involved in cold signal transduction with different mechanisms for low temperature and freezing responses. A genome-wide association study highlighted a phosphoglycerate kinase gene implication in winter bud freezing resistance.
Article
Genetics & Heredity
Chao Lu, Yunxiao Wei, Zhigang Meng, Yongming Liu, Abid Muhammad Ali, Qinfei Liu, Mubashir Abbas, Yanan Wang, Chengzhen Liang, Yuan Wang, Rui Zhang
Summary: By codon-optimizing the LT gene (SV40 large T antigen), this study showed that it can increase the transformation efficiency and shorten the formation time of embryogenic callus in cotton, providing a new strategy for improving cotton transformation and breeding new germplasms.
Article
Biochemistry & Molecular Biology
Elias Hurtado-Gaitan, Susana Selles-Marchart, James Hartwell, Maria Jose Martinez-Esteso, Roque Bru-Martinez
Summary: The study found that under CD induction conditions, PPCK was down-regulated, stilbene production increased, and PPC activity was lost in both cell cultures and leaves. Additionally, STS transcripts were co-induced with MYB14 and WRKY24 in both tissues. These genes had not been previously reported to respond to CD in grape leaves.
Review
Plant Sciences
Luis Alberto Bravo-Vazquez, Paola Isabel Angulo-Bejarano, Anindya Bandyopadhyay, Ashutosh Sharma, Sujay Paul
Summary: Plant regulatory noncoding RNAs (ncRNAs) play a key role in modulating gene expression during callus induction, and further research may lead to innovative plant tissue culture protocols.
PLANT CELL REPORTS
(2023)
Article
Plant Sciences
Olga A. Aleynova, Andrey R. Suprun, Alexey A. Ananev, Nikolay N. Nityagovsky, Zlata V. Ogneva, Alexandra S. Dubrovina, Konstantin V. Kiselev
Summary: This study investigated the effect of overexpression of VaCML genes on stilbene and biomass accumulation in Vitis amurensis cell cultures. The results showed that overexpression of VaCML65 gene significantly increased stilbene content, while overexpression of other VaCML genes had inconsistent effects. The VaCML65 gene is suggested to be a strong positive regulator in the stilbene biosynthesis pathway, which could be useful for obtaining grape cultivars with high stilbene content and stress resistance in viticulture and winemaking.
Article
Biochemistry & Molecular Biology
Nikolay N. Nityagovsky, Konstantin V. Kiselev, Andrey R. Suprun, Alexandra S. Dubrovina
Summary: Recent investigations have shown the potential of using external dsRNA to induce RNA interference (RNAi) and downregulate specific genes in plant fungal pathogens and viruses. This study used exogenous gene-specific dsRNA to silence the chalcone synthase (CHS) gene in Arabidopsis, and the results demonstrated that the exogenous dsRNA was processed into siRNAs and induced gene silencing. The findings of this study highlight the promising application of exogenous dsRNAs for plant management and gene functional studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Olga A. Aleynova, Nikolay N. Nityagovsky, Alexandra S. Dubrovina, Konstantin Kiselev
Summary: This study analyzed the composition of bacterial endophytes in wild Amur grape in the south of the Russian Far East using two different methods. The results showed that the dominant endophytes in V. amurensis were Gammaproteobacteria, Alphaproteobacteria, Actinobacteria, and Bacilli. NGS revealed a greater diversity of classes and genera compared to the cultivation-dependent method. The study also found that the composition of endophytic bacteria varied depending on the plant organ, grape individuals, environmental conditions, and sampling time.
Article
Plant Sciences
Konstantin Kiselev, Andrey R. Suprun, Olga A. Aleynova, Zlata Ogneva, Eduard Y. Kostetsky, Alexandra S. Dubrovina
Summary: Recent investigations have shown that exogenous double-stranded RNAs (dsRNAs) can specifically suppress transgene expression in plants, indicating the potential of using dsRNAs as a promising strategy for plant gene regulation.
Article
Plant Sciences
Olga A. Aleynova, Nikolay N. Nityagovsky, Andrey R. Suprun, Alexey A. Ananev, Alexandra S. Dubrovina, Konstantin Kiselev
Summary: This study investigated the fungal endophytic community of wild grape Vitis amurensis using two different approaches. The results showed a diverse range of fungal taxa, with Dothideomycetes and Tremellomycetes being the most represented classes.
Editorial Material
Plant Sciences
Olga A. A. Aleynova, Konstantin V. V. Kiselev
Article
Biochemistry & Molecular Biology
Andrey R. R. Suprun, Konstantin V. V. Kiselev, Alexandra S. S. Dubrovina
Summary: RNA interference (RNAi) is a natural regulatory mechanism that can be induced artificially by applying dsRNA to plant surfaces. This study investigated the effect of exogenous gene-specific dsRNAs on the silencing of tomato genes related to anthocyanin biosynthesis. The results showed that the application of dsRNAs downregulated the mRNA levels of the target genes, upregulated the expression of anthocyanin biosynthesis-related genes, and increased anthocyanin content in tomato leaves. This demonstrates the potential of using exogenous gene-specific dsRNAs to induce gene silencing and enhance plant secondary metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Forestry
Andrey R. Suprun, Alexandra S. Dubrovina, Valeria P. Grigorchuk, Konstantin V. Kiselev
Summary: This study described the seasonal variations in stilbene compositions and contents in different parts of Pinus koraiensis. HPLC-ESI-MS analysis showed the presence of seven stilbenes in P. koraiensis. The complete protein-coding sequences of three stilbene synthase genes were obtained from the P. koraiensis needles.
Article
Plant Sciences
Konstantin V. Kiselev, Olga A. Aleynova, Zlata V. Ogneva, Andrey R. Suprun, Alexey A. Ananev, Nikolay N. Nityagovsky, Alina A. Dneprovskaya, Alina A. Beresh, Alexandra S. Dubrovina
Summary: Calcium serves as a crucial messenger in plant stress adaptation and developmental processes. The present study aimed to investigate the expression of calcium sensor proteins in grapevine in response to conditions inducing stilbene biosynthesis. The study found that some calcium sensors may be involved in the regulation of secondary metabolite production in grapevine cells.
Article
Horticulture
Konstantin V. Kiselev, Zlata V. Ogneva, Olga A. Aleynova, Andrey R. Suprun, Alexey A. Ananev, Nikolay N. Nityagovsky, Alexandra S. Dubrovina
Summary: Modern plant biotechnology often struggles with obtaining a stable and powerful vector for gene overexpression. It has been found that genes with introns can greatly enhance transgene expression and biological functions in plants and plant cell cultures.
Article
Agricultural Engineering
Yahui Bo, Shanshan Wang, Feifei Ma, Artem Yurevich Manyakhin, Guilin Zhang, Xiaohui Li, Chengxu Zhou, Baosheng Ge, Xiaojun Yan, Roger Ruan, Pengfei Cheng
Summary: Spermidine supplementation promotes cell proliferation and fucoxanthin synthesis in Isochrysis sp., especially under low light intensity.
BIORESOURCE TECHNOLOGY
(2023)
Review
Agricultural Engineering
Shengzhou Shan, Artem Yurevich Manyakhin, Chun Wang, Baosheng Ge, Jichang Han, Xuezhi Zhang, Chengxu Zhou, Xiaojun Yan, Roger Ruan, Pengfei Cheng
Summary: This paper reviews the carbon and energy metabolism of microalgae and reveals the synergistic mechanisms of mixotrophic mode. It explains the shortcomings of photoautotrophic and heterotrophic growth and proposes strategies to improve mixotrophic culture. The findings have important implications for increasing biomass production and improving microalgal culture.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Olga A. Aleynova, Konstantin V. Kiselev, Andrey R. Suprun, Alexey A. Ananev, Alexandra S. Dubrovina
Summary: CMLs are important plant calcium sensor proteins that sense and decode changes in intracellular calcium concentration. This study found that VaCML92 plays a positive regulatory role in the rapid response to cold stress and stress resistance in Vitis amurensis, as well as increased stilbene production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)