Article
Biotechnology & Applied Microbiology
Kateryna Kukil, Elias Englund, Nick Crang, Elton P. Hudson, Pia Lindberg
Summary: In this study, phenylalanine resistant mutant strains of the unicellular cyanobacterium Synechocystis sp. PCC 6803 were generated by laboratory evolution. The mutant strains were able to secrete phenylalanine and showed potential for production of trans-cinnamic acid and para-coumaric acid. The PRM8 strain demonstrated the highest specific production of these compounds.
METABOLIC ENGINEERING
(2023)
Article
Genetics & Heredity
Chen Xu, Bing Wang, Lin Yang, Lucas Zhongming Hu, Lanxing Yi, Yaxuan Wang, Shenglan Chen, Andrew Emili, Cuihong Wan
Summary: In this study, using co-fractionation coupled with LC-MS/MS, the researchers identified 291 multi-protein complexes and 24,092 protein-protein interactions in Synechocystis sp. PCC 6803. These interactions reveal the roles of photosynthesis in various physiological processes and highlight the differences in protein functions between bacteria and higher plants.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Biochemical Research Methods
Ian Sofian Yunus, Zhixuan Wang, Pachara Sattayawat, Jonathan Muller, Fessehaye W. Zemichael, Klaus Hellgardt, Patrik R. Jones
Summary: The study focused on improving the productivity of 1-octanol by enhancing enzyme efficiency and optimizing environmental conditions. By adjusting the inducer concentration and light intensity, the researchers were able to increase the 1-octanol productivity significantly, reaching a carbon partitioning rate of 35% and a titer of 526 mg/L within 12 days.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Microbiology
Jin Ding, Nils Schuergers, Heike Baehre, Annegret Wilde
Summary: This study discovered functional CARF-domain effector and ring nuclease proteins in the cyanobacterium Synechocystis sp. PCC 6803. The Csx1 homolog, SyCsx1, is a strict cytosine-specific RNase that depends on cyclic tetraadenylate (cA4). Another CARF-domain protein similar to Csm6 effectors, SyCsm6, does not have RNase activity but can degrade cOAs and attenuate SyCsx1 RNase activity. This research suggests that the CRISPR systems in Synechocystis have a multi-layered cA4-mediated defense mechanism.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Parveen Akhtar, Avratanu Biswas, Fanny Balog-Vig, Ildiko Domonkos, Laszlo Kovacs, Petar H. Lambrev
Summary: Cyanobacterial mutants with monomeric PSI show changes in the composition and abundance of phycobilisomes and in the excitation energy transfer to PSII and PSI. The oligomeric state of PSI impacts the excitation energy flow in Synechocystis.
Article
Biochemistry & Molecular Biology
Prashant R. R. Singh, Jainendra Pathak, Rajneesh, Haseen Ahmed, Donat -P. P. Haeder, Rajeshwar P. Sinha
Summary: Cyanobacteria use circadian rhythms to cope with daily light fluctuations and the dark phase plays a significant role in protecting them from the negative impact of continuous light exposure. This study provides insights into the physiological responses of cyanobacteria to changing light environments.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2023)
Article
Plant Sciences
Xiang Li, Chunling Xue, Hui Chen, Huafeng Zhang, Qiang Wang
Summary: This study discovered a small antisense RNA gene (ThfR) that interacts with the thf1 gene, regulating photosynthesis under nitrogen starvation or high-light stress, and enhancing the growth ability of cyanobacteria under high-light stress.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lucas Gewehr, Benedikt Junglas, Ruven Jilly, Johannes Franz, Wenyu Eva Zhu, Tobias Weidner, Mischa Bonn, Carsten Sachse, Dirk Schneider
Summary: The dynamin-like protein SynDLP in the cyanobacterium Synechocystis sp. PCC 6803 forms ordered oligomers and interacts with negatively charged thylakoid membrane lipids independently of nucleotides. The structural characteristics of SynDLP suggest it to be a bacterial ancestor of eukaryotic dynamin.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Eleftheria-Angeliki Valsami, Angeliki Pateraki, Anastasios Melis, Demetrios F. Ghanotakis
Summary: This study investigated various cultivation conditions for heterologous beta-phellandrene production by Synechocystis sp. PCC 6803, finding that immobilized cells in calcium alginate beads resulted in higher production compared to suspended cultures. Additionally, extreme conditions of pH 10.5 and 600 mM NaCl proved to be conducive for cell growth and beta-phellandrene production, offering new approaches for sustainable production of terpenoids.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vetaka Tharasirivat, Saowarath Jantaro
Summary: The overexpression of the RuBisCO gene can increase cyanobacterial biomass and improve biocompound production. We successfully constructed two strains of Synechocystis sp. PCC 6803 that overexpressed the rbc gene, resulting in faster growth compared to the wild type strain. The increased biomass of these strains led to higher production of polyhydroxybutyrate (PHB) under nutrient-deprived conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Gang Ruan, Wujuan Mi, Xuwang Yin, Gaofei Song, Yonghong Bi
Summary: This study investigated the physiological response of Synechocystis sp. PCC 6803 to cadmium stress and identified the toxicity targets and response mechanisms. Cadmium was found to damage the cell's photosynthesis and ultrastructure. The study also identified the target sites of cadmium and revealed that the upregulation of the ABC transporter pathway and its protein expression contributed to the response mechanism to cadmium toxicity.
Article
Plant Sciences
Shoki Ito, Atsuko Watanabe, Takashi Osanai
Summary: This study biochemically characterized the L-aspartate oxidase from Synechocystis sp. PCC 6803 and determined its role in NADP+ biosynthesis under photoautotrophic conditions. The results provide important insights into the regulatory mechanism of cyanobacterial NADP+ biosynthesis.
Article
Biotechnology & Applied Microbiology
Xinyu Hu, Tianyuan Zhang, Kai Ji, Ke Luo, Li Wang, Wenli Chen
Summary: This study investigated the response of Synechocystis sp. PCC 6803 to MV-induced oxidative stress using transcriptomic and metabolomic analyses. Results showed that oxidative stress was strongest at 6 hours, with the highest proportion of upregulated genes and metabolites involved in ten metabolic pathways. Photosynthesis positively regulated the response, while environmental information processing was inhibited by MV.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Sang-Hyeok Cho, Yujin Jeong, Seong-Joo Hong, Hookeun Lee, Hyung-Kyoon Choi, Dong-Myung Kim, Choul-Gyun Lee, Suhyung Cho, Byung-Kwan Cho
Summary: This study elucidated the inhibitory mechanisms of photosynthesis in cyanobacteria under high-light and low-temperature stress conditions, highlighting the differences in response mechanisms between the two stress conditions. Through multiomics data analysis and construction of genome-wide transcription unit architecture, factors affecting photosynthesis at transcription, posttranscription, and translation levels were identified, providing pivotal information for future cyanobacterial cell factories development.
Article
Multidisciplinary Sciences
Raquel Garcia-Canas, Joaquin Giner-Lamia, Francisco J. Florencio, Luis Lopez-Maury
Summary: The study reveals the involvement of proteins PetR and PetP in regulating the soluble electron carrier in cells. Transcriptomic analysis shows that the PetRP system specifically regulates four genes (petE, petJ, slr0601, and slr0602), with their presence potentially representing an early adaptation to decreased iron bioavailability following the GOE.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)