期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 173, 期 4, 页码 989-1002出版社
HUMANA PRESS INC
DOI: 10.1007/s12010-014-0901-7
关键词
Hatching enzyme; Collagen gel contraction; MMP; Gene regulation
资金
- Korea Sea Grant Program(GangWon Sea Grant) - Ministry of Oceans and Fisheries in Korea
Various bioactivities of the starfish hatching enzyme (HE) including collagen gel contraction, MMPs activity, hydroxyproline release, and gene regulation based on the fibroblast-populated collagen lattice (FPCL) in three-dimensional medium were investigated for the improvement of scar and keloid. The starfish HE significantly inhibited the collagen gel contraction over 2 days of culture. MMP-2 and MMP-9 activities were also identified by gelatin zymography and RT-PCR products with both HE and collagenase treatments, which resulted in the high amount of hydroxyproline release. The HE treatment on the FPCL significantly inhibited the fibroblast proliferation at 3 days of culture. The LPS-induced NO level and iNOS mRNA expression at low concentrations of HE presented a certain ability to inflammatory response. The COX-2 mRNA from the FPCL indicated no significant inflammation-mediated activity at 5 mu g/mL of HE, whereas the cytokines of TNF-alpha and IL-1 beta were significantly higher than those of the control. Hence, the starfish hatching enzyme can regulate the fibroblast-populated collagen gel conditions by the contraction, MMP production, inflammatory gene expression, etc. Therefore, the starfish HE could be a potential cosmeceutical to heal the scar and keloid tissue.
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