期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 166, 期 2, 页码 259-267出版社
SPRINGER
DOI: 10.1007/s12010-011-9421-x
关键词
Ergothioneine; Histidine; Tyrosinase; DPPH
资金
- National Science Council, Taiwan, ROC [NSC99-2622-B-241-003-CC3]
The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC50 value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompetitive tyrosinase inhibitor using a Lineweaver-Burk plot of experimental kinetic data. The IC50 value for ergothioneine scavenging of 2,2-diphenyl-1-picrylhydrazyl was 6.110+/-0.305 mg/ml, much higher than the IC50 for inhibition of tyrosinase activity which indicating ergothioneine on tyrosinase shows a weak correlation to its antioxidative activity. The results demonstrated that ergothioneine has a potent inhibition effect on tyrosinase enzyme activity, resulting from the presence of the sulfur substituted imidazole ring in ergothioneine.
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