期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 164, 期 1, 页码 23-33出版社
SPRINGER
DOI: 10.1007/s12010-010-9111-0
关键词
Experimental mixture design; Cellulose; Complex formulation; Cellulase; Xylanase
资金
- China Petroleum & Chemical Corporation [207035]
- National Natural Science Foundation of China [20876078, 20936002]
- United Foundation of NSFC and Guangdong Province [U0733001]
- National Hi-tech Research and Development Program of China [2009AA02Z08]
- National Basic Research Program of China [2007CB707805]
To improve the enzymatic hydrolytic efficiency and reduce the supplementation of enzymes, the mixture designed experimental approach was used to optimize the composition of enzyme mixture and promote the hydrolysis of ball-milled corn stover. From the experimental results, a synergistic effect was found when combinations of the three enzymes, two kinds of cellulases and a kind of xylanase, were used. The optimal hydrolysis of pretreated corn stover accorded with enzymes activity ration of FPU/CMCase/beta-glucosidase/xylanase = 4.4:1:75:829, and the hydrolysis efficiency of corn stover increased significantly compared with using individual enzyme. The results indicated that the mixture design experiment could be an effective tool for optimized enzyme mixture for lignocellulose hydrolysis.
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