Article
Plant Sciences
Nikolaos Georgakis, Nirmal Poudel, Dimitrios Vlachakis, Anastassios C. Papageorgiou, Nikolaos E. Labrou
Summary: The study identified the acetanilide herbicide butachlor as a potent and selective inhibitor towards MHR-GSTFs from grass weeds, and determined the crystal structure of LrGSTF in complex with the inhibitor. A specific 3D pharmacophore targeting the MHR-GSTFs was designed, and the role of conserved Tyr118 and adjacent residues in ligand binding and pharmacophore design was revealed. These findings offer new insights for combating MHR weeds.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Microbiology
Anna C. Lienkamp, Jan Burnik, Thomas Heine, Eckhard Hofmann, Dirk Tischler
Summary: This study investigated the styrene-specific degradation pathway in Actinobacterium Gordonia rubripertincta CWB2, characterizing two glutathione S-transferases. The enzymes were shown to be crucial for the ability of CWB2 to produce valuable chemical compounds, suggesting potential for biotechnological applications. The research provides insights into both the specific bacterial degradation pathway and the rare field of actinobacterial glutathione metabolism.
MICROBIOLOGY SPECTRUM
(2021)
Article
Entomology
Siti Nasuha Hamzah, Silas Wintuma Avicor, Zazali Alias, Sarah Abdul Razak, Siti Khadijah Mohd Bakhori, Ting Chuan Hsieh, Nurin Nazifa Syanizam, Salinah Abdul Farouk
Summary: This study aimed to identify the GSTs induced by acute insecticide challenges and analyze the detoxification mechanisms in Aedes albopictus larvae. The findings suggest that Delta and Epsilon GSTs are mainly involved in the detoxification mechanism via direct glutathione metabolism or the sequestration of insecticides, providing protection against oxidative stress induced by insecticide exposure.
Article
Physiology
An-Jin Yang, Ning-Na Yin, Dan-Lu Chen, Yu-Ruo Guo, Yu-Jie Zhao, Nai-Yong Liu
Summary: This study investigated the detoxification mechanisms of the wood-boring beetle Pharsalia antennata through transcriptomics and gene identification. The results revealed differential expression of detoxification genes in different tissues and identified orthologous genes to other species. These findings provide valuable resources for understanding xenobiotic metabolism and odorant detection in P. antennata.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yanjun Liu, Timothy Moural, Sonu B. K. Koirala, Jonathan Hernandez, Zhongjian Shen, Andrei Alyokhin, Fang Zhu
Summary: This study focused on the role of LdGSTu1 in xenobiotic adaptation in the Colorado potato beetle, showing its catalytic activity in conjugating GSH to CDNB and PNA, as well as its potential inhibition by various pesticides. The research provided insights into the molecular mechanisms underlying xenobiotic adaptation and pesticide resistance in this agricultural pest.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Sare Ilknur Yavasoglu, Abeer Mousa Alkhaibari, Fatih Mehmet Simsek
Summary: Ae. caspius populations in Turkey show resistance to multiple insecticides and have multiple resistance mechanisms.
INTERNATIONAL JOURNAL OF PEST MANAGEMENT
(2022)
Article
Engineering, Marine
Lin-Yan Liao, Hui-Ling Cheng, Shu-Yin Wang, Shih-Hsiung Liang, Da-Ji Huang
Summary: This study aimed to investigate the effects of Wuling Farm activities on the water ecosystem by analyzing the biochemical parameters of Onychostoma barbatulum. The results showed that farming activities did not significantly affect the aquatic organisms, but pollution sources were still releasing pollutants containing xenoestrogens into the rivers.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Physiology
Wenhong Li, Xinyi Wang, Po Jiang, Mingwei Yang, Zhimo Li, Chunyang Huang, Yueping He
Summary: This study used different sequencing technologies to reveal the transcriptome profiling and tissue-specific expression patterns of the predatory stink bug P. Lewisi. The results showed that this insect has the potential for controlling Lepidoptera pest insects in crops and forests. The study also identified candidate genes related to detoxification of insecticides and degradation of odors.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Dawei Zhang, Xiangju Li, Feng Bei, Tao Jin, Sisi Jia, Ruotong Bu, Jinxin Wang, Hengzhi Wang, Weitang Liu
Summary: This study identified a herbicide-resistant population of Aegilops tauschii, and explored the non-target site resistance mechanism through transcriptome sequencing and reference genome analysis. The results showed that cytochrome P450 monooxygenase (P450) and glutathione S-transferase (GST) play crucial roles in the enhanced metabolism of mesosulfuron-methyl.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Environmental Sciences
Ahmed A. A. Aioub, Ahmed S. S. Hashem, Ahmed H. H. El-Sappah, Amged El-Harairy, Amira A. A. Abdel-Hady, Laila A. A. Al-Shuraym, Samy Sayed, Qiulan Huang, Sarah I. Z. Abdel-Wahab
Summary: This study identified and characterized GST genes in the fall armyworm S. frugiperda and evaluated the toxicity of emamectin benzoate (EBZ) and chlorantraniliprole (CHP) on the larvae. A total of 31 GST genes were identified, and SfGSTe10 and SfGSTe13 showed the highest expression after EBZ and CHP treatments. Molecular docking analysis revealed high binding affinity between EBZ and CHP with SfGSTe10 and SfGSTe13. These findings provide important insights into the detoxification processes of S. frugiperda regarding EBZ and CHP.
Article
Infectious Diseases
Dong Wang, Liang-Xing Fang, Yu-Wei Jiang, De-Shu Wu, Qi Jiang, Ruan-Yang Sun, Min-Ge Wang, Jian Sun, Ya-Hong Liu, Xiao-Ping Liao
Summary: This study investigated the prevalence and molecular characteristics of fosA3 and fosA7 among Salmonella isolates. The results showed that fosA7 and fosA3 exhibited different levels of resistance and distribution patterns in different strains. Additionally, fosA7 was found to enhance Salmonella survival under stress conditions. This study is important for understanding the mechanisms and molecular characteristics of fosfomycin resistance in Salmonella.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Genetics & Heredity
Yu-Wen Zhao, Chu-Kun Wang, Xiao-Yu Huang, Da-Gang Hu
Summary: Anthocyanins play vital biological roles in plant growth and development, with GSTs being essential for their transportation. The study identified 38 GST genes in the apple genome and found 32 orthologous gene pairs between apple and Arabidopsis. MdGSTU12 was significantly correlated with anthocyanin variation in apple, indicating its importance in anthocyanin accumulation.
Article
Environmental Sciences
Ge-Zi Li, Yong-Xing Zheng, Shi-Juan Chen, Jin Liu, Peng-Fei Wang, Yong-Hua Wang, Tian-Cai Guo, Guo-Zhang Kang
Summary: The study identified a Cu-induced glutathione S-transferase 1 (TaGST1) gene and its transcription factor TaWRKY74 in wheat. TaWRKY74 plays a crucial role in regulating TaGST1 expression and affecting GSH accumulation under Cu stress, providing a potential strategy to alleviate Cu toxicity for crop food safety.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biology
Milos Petrovic, Tatjana Simic, Tatjana Djukic, Tanja Radic, Ana Savic-Radojevic, Milica Zekovic, Otas Durutovic, Aleksandar Janicic, Bogomir Milojevic, Boris Kajmakovic, Marko Zivkovic, Nebojsa Bojanic, Uros Bumbasirevic, Vesna Coric
Summary: This pilot study aimed to assess the relationship between polymorphisms of GSTO1, GSTO2, and GSTO2 and the risk for testicular GCT development. The results showed that carriers of GSTO1*C/A*C/C genotype had an increased risk for testicular GCT, as well as carriers of GSTO2rs2297235*A/G*G/G and GSTO2rs156697*A/G*G/G genotypes. In conclusion, the study suggests that GSTO polymorphisms may affect the antioxidant activity of GSTO isoenzymes, predisposing susceptible individuals to a higher risk for testicular GCT development.
Article
Genetics & Heredity
Qun Yang, Wanjun Yang, Feng Shang, Biyue Ding, Jinzhi Niu, Jinjun Wang
Summary: This study sequenced the transcriptome and small RNA of the invasive Asian tramp snail collected from citrus orchards in southern China. Genes associated with xenobiotic metabolism and core RNAi machinery were identified, providing a foundation for further molecular research on this species and other land snails with agricultural importance.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Anna Christina R. Ngo, Fabian Peter Josef Schultes, Artur Maier, Simon Niklas Hermann Hadewig, Dirk Tischler
Summary: This study investigated the fusion of azoreductase (AzoRo) and formate dehydrogenase (FDH) for efficient reduction of azo dyes. The position of AzoRo in the fusion protein affects enzyme activities, with a 20-fold decrease when it is in the N-terminus. The FDHC23S+AzoRo construct showed the highest activity and improved substrate scope for bulkier dyes. Additionally, pH influenced the stability and activity of the fusion protein, with a suboptimal pH leading to reduced dye reduction.
Article
Chemistry, Medicinal
Fazlin Pheiffer, Yannik K. -H. Schneider, Espen Holst Hansen, Jeanette Hammer Andersen, Johan Isaksson, Tobias Busche, Christian Rueckert, Joern Kalinowski, Leonardo van Zyl, Marla Trindade
Summary: This study investigates the bioactive potential of the marine invertebrate symbiont Thalassomonas actiniarum, identifying its antibacterial and cytotoxic activities, as well as their ability to produce cholic acid and 3-oxo cholic acid through hydrolysis of conjugated bile acids. The findings provide a foundation for further research on the bioactivities and ecological role of Thalassomonas sp. in the marine environment.
Article
Chemistry, Multidisciplinary
Julia Kratky, Daniel Eggerichs, Thomas Heine, Sarah Hofmann, Philipp Sowa, Renato H. Weisse, Dirk Tischler, Norbert Straeter
Summary: This study presents the crystal structure of VpIndA1, an indole monooxygenase from bacterium Variovorax paradoxus EPS, and reveals the structural basis of substrate binding and stereoselectivity through substrate complex structures and force-field calculations. By redesigning the substrate cavity based on the structural information, variants with new substrate selectivity and greatly enhanced stereoselectivity were successfully generated. This research opens up new possibilities for structure-based design of these powerful biocatalysts.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Melissa E. Reardon-Robinson, Minh Tan Nguyen, Belkys C. Sanchez, Jerzy Osipiuk, Christian Ruckert, Chungyu Chang, Bo Chen, Rahul Nagvekar, Andrzej Joachimiak, Andreas Tauch, Asis Das, Hung Ton-That
Summary: In many gram-positive Actinobacteria, MdbA plays a crucial role in bacterial viability by catalyzing oxidative folding of exported proteins. However, in Corynebacterium diphtheriae, the deletion of mdbA only affects cell growth at 37°C, suggesting the existence of alternative oxidoreductase enzymes. Through genetic suppressor mutations, we discovered that elevated expression of tsdA can compensate for the loss of mdbA by mediating oxidative folding of pilin and toxin substrates.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Zehao Zhang, Huijuan Qian, Zhongshi Wang, Ying Pang, Xiaowei Guan, Ansgar Poetsch, Dongmei Wang
Summary: Histone acetylation is an important epigenetic mechanism that plays a role in the regulation of various genes controlling growth, development, and response to environmental stresses in eukaryotes. However, the association of histone acetylation with dehydration stress and the genes involved in histone acetylation and deacetylation in red algae remain largely unknown. In this study, in silico analysis identified several histone acetyltransferase (HAT) and histone deacetylase (HDAC) genes in the red seaweed Pyropia yezoensis. The expression of these genes was found to be upregulated during dehydration, suggesting their potential roles in modulating gene expression under stress conditions. Experimental inhibition of HAT and HDAC activities confirmed their involvement in dehydration adaptation. This study provides insights into the regulation and function of HAT/HDAC during stress adaptation in red algae.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Physical
Fabian Peter Josef Schultes, Melody Haarmann, Dirk Tischler, Carolin Muegge
Summary: Whole-cell biotransformations are a preferred method for complex reactions involving multi-enzyme systems or costly cofactors. However, the toxicity of alcohols, which can serve as reagents, intermediates, or products, poses a challenge for microbial cells. This study investigates the tolerance of different expression strains to alcohols with varying chain lengths, providing important insights for whole-cell biotransformations.
MOLECULAR CATALYSIS
(2023)
Article
Microbiology
Simone Kann, Gustavo Concha, Felix Weinreich, Andreas Hahn, Christian Rueckert, Joern Kalinowski, Olfert Landt, Hagen Frickmann
Summary: This study compared the performance of two commercial real-time PCR assays for identifying Trypanosoma cruzi DNA in serum. The results showed that both assays had a similar diagnostic accuracy for detecting T. cruzi from human serum, with slightly higher specificity observed for the TibMolBiol assay. The RealStar assay exhibited cross-reactions with T. rangeli, a non-pathogenic parasite, which may be a disadvantage in areas where T. cruzi and T. rangeli co-circulate.
Article
Plant Sciences
Zehao Zhang, Junhao Wang, Xiaoqian Zhang, Xiaowei Guan, Tian Gao, Yunxiang Mao, Ansgar Poetsch, Dongmei Wang
Summary: This study successfully isolated nuclear DNA using the Chromatin Immunoprecipitation (ChIP) method and analyzed it through high-throughput sequencing. The results showed that this method can effectively reduce interference from cytosolic DNA and bacterial DNA, providing high-quality reference data for genome assembly.
Article
Microbiology
Daniel Siebert, Tobias Busche, Ezgi Saydam, Jorn Kalinowski, Christian Rueckert-Reed, Bastian Blombach
Summary: Pseudomonas carboxydohydrogena is a lithoautotrophic and obligate aerobic alphaproteobacterium that can utilize CO, CO2, H-2, and mixtures thereof as sole carbon and energy sources. The complete genome sequence of type strain DSM 1083 is reported, and its close relation to Afipia carboxidovorans strain OM5 is discussed.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Review
Biochemistry & Molecular Biology
Shipan Fan, Ansgar Poetsch
Summary: Extracellular vesicles (EVs) are lipid bilayer membranous structures produced and released from various cells, and their versatility has been investigated in different pathologies. Proteomics technologies have revolutionized EV studies by enabling high-throughput analysis of their biomolecules for comprehensive identification and quantification. Extensive research has highlighted variations in EV cargo depending on various factors, sparking activities to utilize EVs for diagnosis and treatment.
Meeting Abstract
Oncology
Cassandra Cieslak, Christian Rueckert, Tobias Busche, Carsten Hain, Joern Kalinowski, Katrin Schaper-Gerhardt, Ralf Gutzmer, Rudolf Stadler
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Biotechnology & Applied Microbiology
Selma Beganovic, Christian Rueckert-Reed, Hilda Sucipto, Wei Shu, Lars Glaeser, Thomas Patschkowski, Ben Struck, Joern Kalinowski, Andriy Luzhetskyy, Christoph Wittmann
Summary: In this study, a multiomics analysis of Streptomyces rimosus was performed to gain insights into the metabolic and regulatory networks driving oxytetracycline formation. The study compared wild-type and hyperproducing strains and revealed key traits influencing oxytetracycline production. Using these traits, the researchers successfully synthesized bhimamycin and created a novel microbial chassis strain with enhanced production. The mutagenized hyperproducer strain exhibited complex mutations, impacting gene expression and complicating rational engineering. This study highlights the importance of precursor supply and genetic stability in improving antibiotic production.
MICROBIAL CELL FACTORIES
(2023)
Article
Chemistry, Physical
Daniel Eggerichs, Kathrin Zilske, Dirk Tischler
Summary: Bacterial eugenol oxidases (EUGOs) have been identified as versatile biocatalysts that can transform lignin degradation products into valuable platform chemicals. In this study, we characterized a new member of the EUGO enzyme family, NspEUGO, and compared its biocatalytic potential with EUGO from Rhodococcus jostii RHA1. NspEUGO showed highest activity on vanillyl alcohol derivatives and exhibited the strongest affinity for eugenol. Immobilization of the enzyme on 14 carrier materials allowed for successful production of vanillin in a packed bed reactor.
MOLECULAR CATALYSIS
(2023)
Article
Infectious Diseases
Leonie Sagurna, Sascha Heinrich, Lara-Sophie Kaufmann, Christian Rueckert-Reed, Tobias Busche, Alexander Wolf, Jan Eickhoff, Bert Klebl, Joern Kalinowski, Julia E. Bandow
Summary: Bacteria of the genus Streptomyces produce various specialized metabolites. Single biosynthetic gene clusters (BGCs) can give rise to different products with varying biological activities. In this study, alnumycin and 1,6-dihydro 8-propylanthraquinone were isolated from a Streptomyces strain containing the alnumycin BGC. Both compounds exhibited antimicrobial activity, with 1,6-dihydro 8-propylanthraquinone showing higher activity against E. coli & UDelta;tolC compared to alnumycin. The modes of action of these compounds are worth further investigation.
Article
Biotechnology & Applied Microbiology
Felix Werner, Lynn S. Schwardmann, Daniel Siebert, Christian Ruckert-Reed, Joern Kalinowski, Marie-Theres Wirth, Katharina Hofer, Ralf Takors, Volker F. Wendisch, Bastian Blombach
Summary: This study achieved efficient fatty alcohol production in C. glutamicum from wheat straw hydrolysate for the first time through targeted metabolic engineering and adaptive laboratory evolution (ALE). The study provides useful metabolic engineering principles to tailor C. glutamicum for other products from second-generation feedstock.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2023)