Article
Multidisciplinary Sciences
Victoria L. Harrod, Russell L. Groves, Matthew A. Maurice, Jeri D. Barak
Summary: Experiments show that tomato leaves damaged by adult Western flower thrips support higher populations of Salmonella enterica, as well as more electrolyte leakage, while plant age and insect gender do not significantly influence the bacterial populations or electrolyte leakage.
Article
Plant Sciences
Xin Liu, Ge Meng, Mengrui Wang, Zilin Qian, Yaxian Zhang, Wencai Yang
Summary: The study characterized the role of the SlPub24 gene in QTL-11B in conferring resistance to bacterial spot in tomato plants. Differential expression between PI 114490 and OH 88119 was due to significant sequence variation in the promoter region.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Xiaofei Zhang, Ning Li, Xin Liu, Jiajing Wang, Yaxian Zhang, Dong Liu, Yuqing Wang, Haipeng Cao, Baimei Zhao, Wencai Yang
Summary: This study elucidates the mechanism of resistance gene Rx4 in tomato against bacterial spot race T3, as well as the potential roles of signaling molecules SGT1-1 and RAR1, providing important clues for addressing challenges in developing resistant varieties.
Review
Biotechnology & Applied Microbiology
Peter Abrahamian, Jeannie M. Klein-Gordon, Jeffrey B. Jones, Gary E. Vallad
Summary: Bacterial spot of tomato caused by Xanthomonas perforans results in significant economic losses globally, with symptoms mainly being necrotic lesions. The pathogen thrives under high humidity and temperature, primarily spreading through wind-driven rain and contaminated seeds. Management primarily relies on copper-based compounds, but resistance is widespread.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Ecology
Paul Runge, Freddy Ventura, Eric Kemen, Remco Stam
Summary: Plants are colonized by a variety of microbes that can influence their development, fitness, and reproduction. This study investigates the microbiota of different tomato species in natural environments. It identifies the core microbiome in wild tomato species and reveals the impact of sampling time, geographical location, host genotype, and plant health on the overall phyllosphere microbiome. Dysbiosis affects the microbial composition in a host species-dependent manner, with healthy leaves having an enrichment of plant-promoting microbial taxa and dysbiotic leaves containing plant pathogens.
Article
Biology
Chandrashekar Srinivasa, Sharanaiah Umesha, Sushma Pradeep, Ramith Ramu, S. M. Gopinath, Mohammad Azam Ansari, Mohammad N. Alomary, Abuzer Ali, Wasim Ahmad, Shiva Prasad Kollur, Chandan Shivamallu
Summary: This study investigated the effect of salicylic acid (SA) on the management of bacterial spot disease in tomatoes caused by Xanthomonas perforans. The results showed that SA treatment significantly improved seed germination and seedling vigor, increased antioxidant enzyme activities, and promoted mRNA accumulation in the resistant variety. These findings indicate that seed priming with SA can effectively elicit defense response against bacterial spot disease in tomato seedlings.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Kimberly N. Cowles, Anna K. Block, Jeri D. Barak
Summary: This study finds that the phytopathogenic bacterium Xanthomonas hortorum pv. gardneri (Xhg) enhances the survival of Salmonella enterica on tomato leaves through its transcription activator-like effector AvrHah1. The AvrHah1 protein is necessary and sufficient for this effect, while Xhg-induced immune response and cellular leakage during disease are independent of AvrHah1.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Mustafa Ojonuba Jibrin, Qingchun Liu, Timothy J. Garrett, Jeffrey B. Jones, Shouan Zhang
Summary: This study evaluated the efficacy of eugenol for managing bacterial spot of tomato caused by X. perforans and identified multiple pathways targeted by eugenol through metabolomic analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
D. Shantharaj, M. A. Williams, N. S. Potnis, M. R. Liles
Summary: Root-associated bacteria produce bioactive metabolites that can mitigate plant diseases. Burkholderia gladioli C101, isolated from soybean rhizosphere, shows potential in inhibiting a broad range of plant pathogens with its heat-stable active secondary metabolites. The application of cell-free supernatants from B. gladioli C101 can reduce disease severity caused by the foliar bacterial pathogen Xanthomonas perforans in a dose-dependent manner, demonstrating its potential in plant disease management.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2021)
Article
Plant Sciences
Elizabeth Teixeira de Almeida Ramos, Fabio Lopes Olivares, Leticia Oliveira da Rocha, Rogerio Freire da Silva, Margarida Gorete Ferreira do Carmo, Maria Teresa Gomes Lopes, Carlos Henrique Salvino Gadelha Meneses, Marcia Soares Vidal, Jose Ivo Baldani
Summary: This study investigated the biotechnological activity of the bacteriocin Gluconacin, which showed antagonistic effects against several important tomato phytopathogens and reduced disease severity. The findings highlight the biotechnological potential of Gluconacin in sustainable agriculture.
Article
Plant Sciences
Kang Qiao, Qingchun Liu, Ye Xia, Shouan Zhang
Summary: NAC can significantly reduce the severity of bacterial spot in tomatoes and enhance the efficacy of copper against copper-resistant X. perforans.
Article
Food Science & Technology
Zhongyue Ren, Lingling Peng, Shufang Chen, Yi Pu, Huihui Lv, Hua Wei, Cuixiang Wan
Summary: Salmonella Typhimurium is commonly found in food and can cause infection in the gastrointestinal tract. This study investigated the effect of a high-fat diet (HFD) on S. Typhimurium infection and the potential protective effects of Lactiplantibacillus plantarum 1201 intervention. The results showed that HFD exacerbated the infection, while intervention with L. plantarum 1201 effectively reduced colonization and inflammation. These findings suggest that L. plantarum 1201 may be a potential preventive strategy against S. Typhimurium infection.
Article
Biochemistry & Molecular Biology
Loic Deblais, Sochina Ranjit, Claudio Vrisman, Linto Antony, Joy Scaria, Sally A. Miller, Gireesh Rajashekara
Summary: Understanding the functional role of bacterial genes in the persistence of Salmonella in plant organs can aid in the development of agricultural practices to mitigate food safety risks associated with contaminated fresh produce. This study found that a specific strain of Salmonella Typhimurium (MDD14) persisted less in tomato plants compared to other tested strains. Whole-genome sequencing identified genetic differences in MDD14, including truncated yicC and mutated rpoS, both involved in stress response and cell envelope integrity. Deleting these genes in another strain confirmed their involvement in persistence. YicC and RpoS could be potential targets to reduce Salmonella persistence in plants.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Microbiology
Eduardo Bernal, Francesca Rotondo, Veronica Roman-Reyna, Taylor Klass, Sujan Timilsina, Gerald V. Minsavage, Fernanda Iruegas-Bocardo, Erica M. Goss, Jeffrey B. Jones, Jonathan M. Jacobs, Sally A. Miller, David M. Francis
Summary: Changes in the race and species composition of Xanthomonas causing bacterial spot of tomato have been observed worldwide. In this study, X. perforans was found to be the most prevalent species in processing tomato fields in the Midwest United States. Sequence variation analysis and genomic comparisons revealed that the majority of X. perforans strains collected between 2017 and 2020 in the Midwest were highly similar to strains collected in Florida in 2012. The persistence of these nearly identical strains suggests that migration has led to the establishment of an endemic population.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiao Pan, Weiyi Li, Jiahao Ni, Kun Wu, Iain Konigsberg, Caitlyn E. Rivera, Clayton Tincher, Colin Gregory, Xia Zhou, Thomas G. Doak, Heewook Lee, Yan Wang, Xiang Gao, Michael Lynch, Hongan Long
Summary: This study provides a systematic understanding of mutation rates and molecular spectra in a pathogen, showing low mutation rates and high genome stability. The study also suggests that elevated mutation rates may not be the major determinant for the diversification of toxin and secretion systems, and highlights the potential differences in accuracy between replication and transcription machinery.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Microbiology
S. Timisina, S. Kara, M. A. Jacques, N. Potnis, G. Minsavage, G. E. Vallad, J. B. Jones, M. Fischer-Le Saux
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2019)
Article
Microbiology
Sujan Timilsina, Juliana A. Pereira-Martin, Gerald Minsavage, Fernanda Iruegas-Bocardo, Peter Abrahamian, Neha Potnis, Bryan Kolaczkowski, Gary E. Vallad, Erica M. Goss, Jeffrey B. Jones
FRONTIERS IN MICROBIOLOGY
(2019)
Review
Microbiology
Shi-Qi An, Neha Potnis, Max Dow, Frank-Joerg Vorhoelter, Yong-Qiang He, Anke Becker, Doron Teper, Yi Li, Nian Wang, Leonidas Bleris, Ji-Liang Tang
FEMS MICROBIOLOGY REVIEWS
(2020)
Article
Microbiology
Eric Newberry, Rishi Bhandari, Joseph Kemble, Edward Sikora, Neha Potnis
ENVIRONMENTAL MICROBIOLOGY
(2020)
Review
Plant Sciences
Neha Potnis
Summary: Bacterial spot is an endemic seedborne disease causing recurring outbreaks on tomatoes and peppers worldwide. The disease is currently managed through chemical/biological controls and disease-resistant pepper cultivars, but has not reduced the incidence of outbreaks. There has been a significant shift in the composition and prevalence of different species and races of the pathogen over the past century.
ANNUAL REVIEW OF PHYTOPATHOLOGY, VOL 59, 2021
(2021)
Article
Plant Sciences
Prabha Liyanapathiranage, Jeffrey B. Jones, Neha Potnis
Summary: This study reveals the significant role of the tssM gene of the T6SS-i3* in the initial asymptomatic colonization of Xanthomonas perforans. The presence of functional TssM contributes to higher aggressiveness and in planta growth of the pathogen, as well as increased epiphytic fitness and dissemination potential. Higher osmotolerance is one mechanism by which TssM enhances epiphytic fitness.
Article
Plant Sciences
Nay C. Dia, Lucas Moriniere, Bart Cottyn, Eduardo Bernal, Jonathan M. Jacobs, Ralf Koebnik, Ebrahim Osdaghi, Neha Potnis, Joel F. Pothier
Summary: The passage provides information about the taxonomy, host range, epidemiology, and control measures of Xanthomonas hortorum.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Sadegh Zarei, S. Mohsen Taghavi, Touraj Rahimi, Hamzeh Mafakheri, Neha Potnis, Ralf Koebnik, Marion Fischer-Le Saux, Joel F. Pothier, Ana Palacio Bielsa, Jaime Cubero, Perrine Portier, Marie-Agnes Jacques, Ebrahim Osdaghi
Summary: A recent study evaluated the taxonomic status of Xanthomonas arboricola, a group of fruit tree pathogens. The study used DNA similarity indexes and core genome-based phylogeny to determine the members of the species. The results revealed that two pathovars should be elevated to the rank of a new species. Additionally, other mislabeled strains belong to previously described species or represent novel species awaiting formal description.
Article
Plant Sciences
Eric A. Newberry, Gerald V. Minsavage, Auston Holland, Jeffrey B. Jones, Neha Potnis
Summary: Xanthomonas perforans and X. euvesicatoria are the causal agents of bacterial spot disease of tomato and pepper, respectively. Recent studies have revealed variability within these bacterial species in causing disease in pepper, even without avirulence gene activity. Through genome-wide association analyses, two novel gene candidates associated with pepper-pathogenic X. perforans and X. euvesicatoria were identified. Functional analysis showed that one candidate had a minor effect on symptom development and growth, suggesting that pathogenicity to pepper may have evolved independently within the bacterial species.
Editorial Material
Microbiology
Kathryn C. Milligan-McClellan, Jose Pablo Dundore-Arias, Jonathan L. Klassen, Ashley Shade, Linda L. Kinkel, Benjamin E. Wolfe
Summary: The diversity and functional significance of microbiomes have been increasingly understood through extensive sampling and new sequencing technologies. This collection of mSystems Perspective pieces reflects the main challenges and opportunities in the field identified by workshop participants. Borrowing approaches from other fields, such as economics and philosophy, these pieces suggest new ways to understand microbiome patterns and processes. Conceptual advances, technological advancements, and integration across scales are improving our understanding of microbiome diversity and function. This integrative microbiome research is happening at a crucial moment where collaboration is needed to support and train a diverse and inclusive community of microbiome scientists.
Article
Plant Sciences
Natalie A. Henkhaus, Wolfgang Busch, Angela Chen, Adan Colon-Carnnona, Maya Cothran, Nicolas Diaz, Jose Pablo Dundore-Arias, Michael Gonzales, Denita Hadziabdic, Rebecca A. Hayes, Gustavo C. MacIntosh, Ali Na, Blessing Nyannasoka-Magonziwa, Dianne Pater, F. Christopher Peritore-Galve, Tara Phelps-Durr, Kerry Rouhier, Delanie B. Sickler, John H. Starnes, Quentin R. Tyler, Evelyn Valdez-Ward, Miguel E. Vega-Sanchez, Ron R. Walcott, Joy K. Ware, Sarah E. Wyatt, Felipe Zapata, Ash T. Zemenick, David B. Stern
Summary: A future where scientific discoveries are valued and trusted by the public requires greater inclusion and participation. Strategies and recommendations were developed by a diverse group to promote equity, diversity, and inclusion in the plant science community. These recommendations, applicable to other disciplines, aim to support systemic change and give voice to unheard perspectives. The vision is for a barrier-free access to science and a diverse plant science community that invests in the training and well-being of all members.
Article
Plant Sciences
David W. Armitage, Morgan E. Carter, Robin A. Choudhury, Mitja N. P. Remus-Emsermann, Cindy E. Morris, Johan H. Leveau, Linda L. Kinkel, Jose Pablo Dundore-Arias
Summary: In this article, we summarize the main takeaways from a symposium and hybrid virtual and in-person participatory discussion focused on the challenges of scale in understanding the ecology and management of phyllosphere microbial communities. We provide an overview of the confounding effects of spatial scale on inference in microbial ecology, the spatial organization of microbial interactions in the phyllosphere, advances and remaining gaps in measuring phyllosphere colonization across scales, and the epidemiology in the phyllosphere. We hope to motivate further discussion and the development and adoption of creative approaches to solving the challenges of scale to enhance fundamental understanding and practical management of the phyllosphere microbiomes.
PHYTOBIOMES JOURNAL
(2023)
Article
Ecology
Rishi Bhandari, Alvaro Sanz-Saez, Courtney P. Leisner, Neha Potnis
Summary: Though much research has been carried out on the response of plants to biotic and abiotic stresses, little is known about the resilience of associated microbiomes and their contribution to stress tolerance or response. This study evaluated the impact of elevated tropospheric ozone (O-3) and Xanthomonas perforans infection on resistant and susceptible pepper cultivars, and found that while the infection resulted in distinct microbial community structure and functions on the susceptible cultivar, concurrent O-3 stress did not further alter the community structure and function. However, O-3 stress did exacerbate the disease severity on the resistant cultivar. The altered microbial co-occurrence network under simultaneous O-3 stress and pathogen challenge may explain the increased disease severity.
ISME COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Jose Pablo Dundore-Arias, Sarah C. Castle, Laura Felice, Ruth Dill-Macky, Linda L. Kinkel
FRONTIERS IN MOLECULAR BIOSCIENCES
(2020)
Article
Plant Sciences
J. P. Dundore-Arias, E. A. Eloe-Fadrosh, L. M. Schriml, G. A. Beattie, F. P. Brennan, P. E. Busby, R. B. Calderon, S. C. Castle, J. B. Emerson, S. E. Everhart, K. Eversole, K. E. Frost, J. R. Herr, A. I. Huerta, A. S. Iyer-Pascuzzi, A. K. Kalil, J. E. Leach, J. Leonard, J. E. Maul, B. Prithiviraj, M. Potrykus, N. R. Redekar, J. A. Rojas, K. A. T. Silverstein, D. J. Tomso, S. G. Tringe, B. A. Vinatzer, L. L. Kinkel
PHYTOBIOMES JOURNAL
(2020)