Article
Biotechnology & Applied Microbiology
Lei Sun, Yingguo Bai, Xiu Zhang, Cheng Zhou, Jie Zhang, Xiaoyun Su, Huiying Luo, Bin Yao, Yuan Wang, Tao Tu
Summary: Three recombinant GAD genes from Bacillus strains were identified and successfully expressed heterologously in Escherichia coli. Using the engineered E. coli strain as a whole-cell biocatalyst, the biosynthesis of GABA from L-Glu was achieved, showing potential for industrial production of GABA.
MICROBIAL CELL FACTORIES
(2021)
Article
Microbiology
Luca Freddi, Jorge A. de la Garza-garcia, Sascha Al Dahouk, Alessandra Occhialini, Stephan Koehler
Summary: This study reveals that atypical Brucella species can grow under anoxic conditions strictly dependent on nitrate, while classical species exhibit different behavior. Atypical Brucella species have a more efficient denitrification pathway and rapid consumption of nitrite, which may be related to their ability to colonize different hosts and rapid growth.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Ying Wang, Jiazhuo Li, Zhiwei Zhou, Ronghui Zhou, Qun Sun, Peng Wu
Summary: Researchers have developed a photosensitizer that can work in extremely acidic environments to combat aciduric bacteria, demonstrating inhibition of bacterial growth in acidic beverages and successful photodynamic inactivation of Candidiasis.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Nize Otaru, Kun Ye, Denisa Mujezinovic, Laura Berchtold, Florentin Constancias, Fabian A. Cornejo, Adam Krzystek, Tomas de Wouters, Christian Braegger, Christophe Lacroix, Benoit Pugin
Summary: Research investigated GABA production of Bacteroides and its protective role in acid stress, revealing the widespread presence of the GAD system in Bacteroides and its potential as the only amino acid-dependent acid tolerance system in the genus.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Sultana Razia, Tony Hadibarata, Sie Yon Lau
Summary: Acidophiles are microorganisms that can survive in highly acidic environments, which are normally inhospitable to other organisms. This review article discusses the roles of acidophiles in removing heavy metals, radioactive pollutants, and acidic organic pollutants from acidic environments. It provides valuable insights for further research in the field of acidic pollutants remediation using acidophilic microorganisms.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Microbiology
Raul Sauceda-Becerra, Hugo Barrios-Garcia, Julio Martinez-Burnes, Beatriz Arellano-Reynoso, Alejandro Benitez-Guzman, Rigoberto Hernandez-Castro, Jorge Alva-Perez
Summary: The objective of this study was to evaluate the transcription of the invA gene in acidic conditions in Brucella melitensis. The results showed that invA gene transcription was upregulated at both pH 3 and pH 5, with lower levels at pH 3 compared to pH 5, similar to the transcription levels of the dnaK gene. The transcription of the invA gene under acidic conditions promotes the survival of Brucella melitensis.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Wenchao Deng, Zihao Zhao, Yufang Li, Rongguang Cao, Mingming Chen, Kai Tang, Deli Wang, Wei Fan, Anyi Hu, Guangcheng Chen, Chen-Tung Arthur Chen, Yao Zhang
Summary: This study provides in-depth insights into the impact of high temperature and low pH on the metabolic processes of energy and main elements in chemolithoautotrophs living in hydrothermal ecosystems, as well as the mechanisms they use to adapt to the extreme hydrothermal conditions.
Article
Biology
David N. Fisher, Rebecca J. LeGrice, Christina J. Painting
Summary: Social selection occurs when traits of interaction partners influence an individual's fitness, but its strength is limited and influenced by few factors. At high densities, social selection was negative for both sexes, but it will not impede the evolution of larger body sizes.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2021)
Article
Food Science & Technology
Juo-Yu Chen, Yuan-Tay Shyu, Sz-Jie Wu
Summary: Passion fruit, also known as Passiflora edulis, is a popular fruit consumed fresh or processed. The discarded shells, which make up more than half of the fruit, contain gamma-aminobutyric acid (GABA) with proven medical benefits. This study focused on increasing GABA content in passion fruit shells through an optimal anoxic vacuum treatment.
JOURNAL OF FOOD SCIENCE
(2023)
Article
Environmental Sciences
Tipei Jia, Liang Zhang, Shihao Sun, Qi Zhao, Yongzhen Peng
Summary: In this study, acidophilic mixotrophic sulfur-oxidizing bacteria (SOB) were enriched under extremely acidic conditions to enhance bio-desulfurization performance. The addition of organics improved the H2S removal efficiency. Maintaining an appropriate biofilm mass is crucial for optimal desulfurization performance. The findings of this study offer a novel strategy for enhancing H2S removal.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Rui Tang, Wei Sun, Ji-Chun Zhang, Liufeng Mao, Natalie Quanquin, Donghai Wu, Yirong Sun
Summary: This research shows that UCP1 can uncouple oxidative phosphorylation in E. coli, increasing membrane permeability and reducing ATP synthesis. UCP1 regulates intracellular pH by controlling proton pumping and affects the acid resistance of E. coli.
CURRENT MICROBIOLOGY
(2022)
Review
Immunology
Rupal Rai, Vinayak Singh, Bijina J. Mathew, Anirudh K. Singh, Shivendra K. Chaurasiya
Summary: Understanding the mechanisms of acid resistance/tolerance in Mycobacterium tuberculosis is crucial for combating tuberculosis. These mechanisms play an important role in the pathogenesis of the disease and may serve as promising targets for controlling mycobacterial infections.
PATHOGENS AND DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Michael Kovermann, Alessandra Stefan, Chiara Palazzetti, Fabian Immler, Fabrizio Dal Piaz, Luca Bernardi, Valentina Cimone, Maria Laura Bellone, Alejandro Hochkoeppler
Summary: The low-molecular weight protein tyrosine phosphatase (MptpA) of Mycobacterium tuberculosis plays a crucial role in inhibiting phagosome-lysosome fusion and contributing to bacterial pathogenicity. The enzyme undergoes conformational changes and decreased catalytic efficiency in acidic pH conditions. Competitive inhibitors of MptpA are more effective at acidic pH values.
Article
Chemistry, Multidisciplinary
Weirui Chen, Hengxi He, Ruini Zou, Yidan Chen, Xukai Li, Jing Wang, Yiming Tang, Laisheng Li
Summary: CeOx with different exposed facets were prepared to investigate their effect on activating the peroxone reaction. The study found that (111) CeOx exhibited better activity than (100) or (110) CeOx due to its ability to generate OVs-OOH. This was attributed to the electron-donating ability of the OVs rather than solution pH. The study also showed that the synergy effect of (111) CeOx/O3 and H2O2 varied with the structure of pharmaceuticals during removal processes.
ENVIRONMENTAL SCIENCE-NANO
(2021)
Article
Agronomy
Ramanjeet Kaur, Vikramjit Kaur Zhawar, Gurpreet Kaur, Bavita Asthir
Summary: The study showed that exogenous GABA treatment can enhance protein content, GAD and DAO activities, as well as proline content in rice cultivar CSR 43, improving stress tolerance in rice seedlings.
CEREAL RESEARCH COMMUNICATIONS
(2021)
Editorial Material
Microbiology
Daniela De Biase, John P. Morrissey, Conor P. O'Byrne
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Peter A. Lund, Daniela De Biase, Oded Liran, Ott Scheler, Nuno Pereira Mira, Zeynep Cetecioglu, Estefania Noriega Fernandez, Sara Bover-Cid, Rebecca Hall, Michael Sauer, Conor O'Byrne
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Daniela De Biase, Francesca Cappadocio, Eugenia Pennacchietti, Fabio Giovannercole, Antonio Coluccia, Jouko Vepsalainen, Alex Khomutov
COMMUNICATIONS CHEMISTRY
(2020)
Editorial Material
Cardiac & Cardiovascular Systems
Daniela De Biase, Giuseppe Biondi-Zoccai, Francesco Versaci, Giacomo Frati
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2021)
Review
Microbiology
Brankica Z. Lakicevic, Heidy M. W. den Besten, Daniela De Biase
Summary: This review provides an up-to-date overview of clonal and serotype-specific differences among Listeria monocytogenes strains, emphasizing the genes and genomic islands responsible for virulence and resistance to environmental stresses. It also highlights the use of advanced diagnostic technologies, such as whole-genome sequencing, for better control of listeriosis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Jorge A. de la Garza-Garcia, Safia Ouahrani-Bettache, Sebastien Lyonnais, Erika Ornelas-Eusebio, Luca Freddi, Sascha Al Dahouk, Alessandra Occhialini, Stephan Koehler
Summary: Our study focused on identifying and characterizing acid resistance determinants of B. suis and B. microti at different pH levels, revealing species-specific mechanisms in response to acid stress. The results showed that B. microti has rapid physiological adaptation to acid stress with specific differential gene expression, while B. suis response indicated increased protein misfolding under acid stress. The findings support the hypothesis that the enhanced acid stress resistance of B. microti is due to selective pressure for critical gene functionality and specific differential gene expression, leading to rapid adaptation.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Cardiac & Cardiovascular Systems
Aysenur Gunaydin Akyildiz, Giuseppe Biondi-Zoccai, Daniela De Biase
Summary: The microbiota of the gastrointestinal tract plays a crucial role in cardiovascular disease and its dysbiosis may contribute to its initiation and progression. Studying the functions and effects of gastrointestinal microbiota is of great significance for improving risk prediction, prevention, and treatment of CVD.
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2022)
Review
Microbiology
Alessandra Occhialini, Dirk Hofreuter, Christoph-Martin Ufermann, Sascha Al Dahouk, Stephan Kohler
Summary: The genus Brucella consists of twelve species of intracellular bacteria with varying zoonotic potential. While six of them are considered classical and known to cause brucellosis in terrestrial mammals, the remaining two species originate from marine mammals. Recent research has led to the discovery of four new species, as well as numerous strains isolated from a wide range of hosts, including cold-blooded animals. These newly identified species exhibit atypical phenotypes, such as higher growth rate, increased resistance to acid stress, motility, and lethality in murine infection models. This review provides an overview of the latest knowledge on these novel Brucella species, highlighting their phylogenetic positions, metabolic characteristics, acid stress resistance mechanisms, and behavior in well-established infection models. The comparison between classical and novel Brucella species and strains leads to a proposed more adapted terminology, distinguishing between core and non-core, and typical versus atypical brucellae.
Editorial Material
Microbiology
Aleksandra Djukic-Vukovic, Nuno P. Mira, Jana Sedlakova-Kadukova, Daniela De Biase, Peter A. Lund
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Tamir Azizi, Laurine Carvalho De Araujo, Zeynep Cetecioglu, Aisha J. Clancy, Marie L. Feger, Oded Liran, Conor O'Byrne, Immanuel Sanka, Ott Scheler, Jana Sedlakova-Kadukova, Carmit Ziv, Daniela De Biase, Peter A. Lund
Summary: This article presents the work of our COST Action on understanding and exploiting the impacts of low pH on microorganisms. It includes a summary of a workshop on the industrial applications of low pH stress on microbial bio-based production and a survey on developing links between academia and industry. The article also introduces two resources developed as part of the COST Action goals to facilitate research and collaboration in this field. Researchers are invited to take advantage of these resources to identify relevant literature and establish collaborations.
Article
Biochemistry & Molecular Biology
Maxim A. Khomutov, Fabio Giovannercole, Laura Onillon, Marija V. Demiankova, Byazilya F. Vasilieva, Arthur I. Salikhov, Sergey N. Kochetkov, Olga V. Efremenkova, Alex R. Khomutov, Daniela De Biase
Summary: The dipeptide derivative L-Leu-D,L-Glu-gamma-PH exhibits promising antibacterial activity against E. coli and Bacillus subtilis, surpassing other similar compounds. This study highlights the importance of the phosphinic pharmacophore in antibacterial activity and suggests new avenues for the development of novel antimicrobial drugs.
Article
Microbiology
Luca Freddi, Jorge A. de la Garza-garcia, Sascha Al Dahouk, Alessandra Occhialini, Stephan Koehler
Summary: This study reveals that atypical Brucella species can grow under anoxic conditions strictly dependent on nitrate, while classical species exhibit different behavior. Atypical Brucella species have a more efficient denitrification pathway and rapid consumption of nitrite, which may be related to their ability to colonize different hosts and rapid growth.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Multidisciplinary
Francesca Petronella, Daniela De Biase, Federica Zaccagnini, Vanessa Verrina, Seok-In Lim, Kwang-Un Jeong, Selenia Miglietta, Vincenzo Petrozza, Viviana Scognamiglio, Nicholas P. Godman, Dean R. Evans, Michael McConney, Luciano De Sio
Summary: The growing spread of pathogens due to human activities has led to increasing interest in the development of rapid, simple, and on-site pathogen detection biosensors with improved performance. In this study, a label-free gold nanorod array was presented and discussed for the rapid detection of Escherichia coli cells in water, based on localized surface plasmon resonance (LSPR). The results showed that the antibody-functionalized gold nanorod array could detect E. coli cells with high sensitivity and excellent optical and morphological properties.
ENVIRONMENTAL SCIENCE-NANO
(2022)
Proceedings Paper
Engineering, Aerospace
Francesca Petronella, Melissa De Angelis, Daniela DeBiase, Seok-Im Lim, Kwang-Un Jeong, Nicholas Godman, Dean Evans, Michael Mcconney, Luciano De Sio
Summary: The researchers developed an innovative and miniaturized biosensor based on nanotechnology to detect and quantify harmful pathogens in potable water. By utilizing gold nanorods functionalized with anti-E.coli antibody, the biosensor showed excellent capability to discriminate small changes in E.coli concentration.
2021 IEEE 8TH INTERNATIONAL WORKSHOP ON METROLOGY FOR AEROSPACE (IEEE METROAEROSPACE)
(2021)
Article
Immunology
Safia Ouahrani-Bettache, Maria P. Jimenez De Bagues, Jorge De La Garza, Luca Freddi, Juan P. Bueso, Sebastien Lyonnais, Sascha Al Dahouk, Daniela De Biase, Stephan Kohler, Alessandra Occhialini