Article
Microbiology
Robert Radisch, Miroslav Patek, Barbora Kristkova, Margit Winkler, Vladimir Kren, Ludmila Martinkova
Summary: This review focuses on the contribution of enzymes to plant-bacteria interactions, specifically discussing substrate specificities, taxonomic and ecological distribution, and importance in selected plant symbionts. The data shows that these enzymes are abundant in Actinobacteria and Proteobacteria and play important roles in breaking through plant defenses, utilizing aldoximes or nitriles as nutrients, and potentially contributing to the synthesis of the phytohormone indole-3-acetic acid.
Article
Plant Sciences
Haimiao Zhang, Zixuan Rong, Yang Li, Ziyi Yin, Chongchong Lu, Haipeng Zhao, Lingguang Kong, Lun Meng, Xinhua Ding
Summary: This study reveals the existence of a nitrilase-dependent IAA biosynthesis pathway in Xanthomonas oryzae pv. oryzicola (Xoc). Genes related to IAA synthesis promote Xoc colonization by inhibiting rice immune defense response and increasing IAA content in Xoc, which affects rice growth.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Huo-Yong Jiang, Hong-Kai Wu, Pan-Pan Yuan, Jing-Jing Guo, Li Wang, Yi-Jun Dai
Summary: A novel isolated bacterium, Pseudomonas stutzeri CGMCC 22915, was found to rapidly degrade Sulfoxaflor (SUL), a sulfoximine insecticide, into SUL-amide. This bacterium effectively facilitated SUL degradation in soil and surface water. Additionally, a nitrile hydratase with a different gene orientation, PsNHase, was discovered to transform SUL and other nitrile-containing insecticides into their respective amides. This research provides potential solutions for the bioremediation of SUL in contaminated soil and surface water.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2022)
Article
Agricultural Engineering
Zhongshi Huang, Xinglong Wang, Ning Li, Fuqiang Song, Jingwen Zhou
Summary: In this study, a whole-cell catalysis method for efficient synthesis of NR was established using E. coli. The researchers confirmed the high catalytic activity of UshA in synthesizing NR and identified the genes involved in NR degradation. They also demonstrated the important role of UshA's signal peptide in NR transport. By introducing nitrile hydratase, they achieved the conversion of 3-cyanopyridine to NR. The efficient biosynthesis of NR in E. coli was achieved with high titers using nicotinamide and 3-cyanopyridine as substrates in a 5-L bioreactor.
BIORESOURCE TECHNOLOGY
(2023)
Article
Microbiology
Shuxue Zhao, Xi Chen, Qianshu Sun, Fei Wang, Chunhui Hu, Lizhong Guo, Jie Bai, Hao Yu
Summary: This study elucidated the molecular mechanisms of IAA degradation by the Pseudomonas sp. LY1 strain at the protein level using label-free quantitative proteomic analysis. The results identified an iad gene cluster in strain LY1 and related degradation pathway genes, confirming their role in IAA degradation and suggesting the existence of multiple degradation pathways. Additionally, the study indicated oxidative stress in strain LY1 during IAA degradation, leading to the upregulation of various proteins to respond to the stress.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jishma Panichikkal, Deepa P. Mohanan, Sreekanth Koramkulam, Radhakrishnan E. Krishnankutty
Summary: This study utilized chitosan nanoparticles (CNPs) to enhance the plant beneficial properties of rhizospheric Pseudomonas monteilii and demonstrated the positive impact of CNPs on IAA production and plant growth.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Article
Plant Sciences
Adrian Pintado, Hilario Dominguez-Cervan, Victoria Pastor, Marissa Vincent, Soon Goo Lee, Victor Flors, Cayo Ramos
Summary: The iaaL alleles in P. savastanoi determine the production of IAA-Lys. iaaL(Psn) and iaaL(Psf) genes control the production of IAA-Lys in pv. savastanoi and pv. fraxini, respectively. Overexpression of these genes reduces the free IAA content and alleviates the inhibitory effect of IAA on plant root growth.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Microbiology
Jintian Tang, Yukang Li, Leilei Zhang, Jintao Mu, Yangyang Jiang, Huilan Fu, Yafen Zhang, Haifeng Cui, Xiaoping Yu, Zihong Ye
Summary: Indole-3-acetic acid (IAA) is an important plant hormone that regulates plant growth and development. It also plays a significant role in microorganisms, affecting their growth, development, and interaction with plants. Understanding the biosynthesis and functions of IAA in microorganisms can help improve its production and utilization in agriculture.
Article
Engineering, Environmental
Ning Xu, Yue Song, Chenfan Zheng, Shan Li, Zhen Yang, Meng Jiang
Summary: Recent research has found that polystyrene nanoplastics (PS-NPs) can hinder the growth of plants and crops, including rice. This study aimed to examine the impact of PS-NPs of different sizes and charges on rice growth, as well as investigate the underlying mechanisms and potential strategies to mitigate their effects. The results showed that positively charged PS-NPs (80 nm PS-NH2) had the most significant negative impact on rice growth, reducing dry biomass, root length, and plant height.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Microbiology
Maria D. Perez, Alfonso Olaya-Abril, Purificacion Cabello, Lara P. Saez, M. Dolores Roldan, Conrado Moreno-Vivian, Victor M. Luque-Almagro
Summary: This study investigates the response of the bacterium P. pseudoalcaligenes CECT5344 to 3-cyanoalanine and the role of the novel nitrilase NitC in the assimilation pathway. The results suggest that Nit4 is not involved in the assimilation of 3-cyanoalanine in the absence of cyanide, while NitC participates in an alternative pathway.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Beatriz Sanchez-Parra, Marta-Marina Perez-Alonso, Paloma Ortiz-Garcia, Jose Moya-Cuevas, Mathias Hentrich, Stephan Pollmann
Summary: The combination of the two mutations is not sufficient to fully prevent the conversion of IAM to IAA, and the detected strong accumulation of IAM significantly impairs seed development. Genome-wide expression studies reveal that the double mutant is broadly affected in its translational capacity, with a small number of plant growth regulating transcriptional circuits being repressed by the high IAM content in the seed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Hongxuan Wu, Jinhua Yang, Peijie Shen, Qingchen Li, Weibin Wu, Xianzhang Jiang, Lina Qin, Jianzhong Huang, Xiao Cao, Feng Qi
Summary: Two novel IAA biosynthesis pathways were developed in engineered Escherichia coli for IAA production, with the IAM pathway exhibiting the highest yield. Optimization of de novo IAA biosynthesis by improving NAD(P)H availability resulted in increased IAA production, showcasing the potential for industrial applications.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Umi Miyata, Kenta Arakawa, Mami Takei, Tadao Asami, Kazuya Asanbou, Hiroaki Toshima, Yoshihito Suzuki
Summary: PonAAS2, an aromatic aldehyde synthase, plays a key role in the biosynthesis of IAA in galling sawfly, with high expression levels in early and mid-stage larvae. Inhibiting PonAAS2 can significantly reduce IAA production in sawfly enzymes, highlighting the enzyme's important role in IAA biosynthesis in sawfly.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Beibei Qi, Chao Wu, Huiling Liang, Kehui Cui, Shah Fahad, Manlian Wang, Baoyu Liu, Lixiao Nie, Jianliang Huang, Hui Tang
Summary: The study developed a method for determining phytohormones using liquid-liquid extraction and HPLC-UV analysis system, with optimized parameters. The method can simultaneously determine 11 phytohormones, showing low detection limits and good recovery rates, suitable for phytohormone determination in rice plants.
Article
Environmental Sciences
Anukool Vaishnav, Roshan Kumar, Harikesh Bahadur Singh, Birinchi Kumar Sarma
Summary: The continuous release of nitrile compounds into agricultural land has led to increased nitrile pollution, which can have negative effects on soil health and crop productivity. The utilization of plant growth-promoting rhizobacteria (PGPR) with nitrilase activity has emerged as a potential solution. This paper reviews the issue of nitrile pollution in soil and discusses the prospects of using nitrile-degrading bacteria for soil remediation and promoting plant growth in nitrile-polluted soils.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)